##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146503_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3723909
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34672812896341	31.0	31.0	34.0	30.0	34.0
2	31.568124785004144	31.0	31.0	34.0	30.0	34.0
3	31.75729482111405	33.0	31.0	34.0	30.0	34.0
4	35.35617384850167	37.0	35.0	37.0	33.0	37.0
5	35.46768462924309	37.0	35.0	37.0	33.0	37.0
6	35.46903267507342	37.0	35.0	37.0	33.0	37.0
7	35.390311363677256	37.0	35.0	37.0	33.0	37.0
8	35.375592421834156	37.0	35.0	37.0	33.0	37.0
9	37.05098835658981	39.0	38.0	39.0	34.0	39.0
10-11	37.03478683286836	39.0	38.0	39.0	34.0	39.0
12-13	37.00016998267144	39.0	38.0	39.0	34.0	39.0
14-15	38.43951933304493	41.0	39.0	41.0	34.0	41.0
16-17	38.45967825744399	41.0	39.0	41.0	34.0	41.0
18-19	38.482465736944704	41.0	39.0	41.0	34.0	41.0
20-21	38.56888191950985	41.0	39.0	41.0	34.0	41.0
22-23	38.59689280806808	41.0	39.0	41.0	34.0	41.0
24-25	38.65178405272524	41.0	39.0	41.0	34.0	41.0
26-27	38.67585096735715	41.0	39.0	41.0	34.0	41.0
28-29	38.63666244261071	41.0	39.0	41.0	34.0	41.0
30-31	38.61742405628064	41.0	39.0	41.0	34.0	41.0
32-33	38.624534326698104	41.0	39.0	41.0	34.0	41.0
34-35	38.57527788675824	40.5	39.0	41.0	34.0	41.0
36-37	38.49719206350102	40.0	38.0	41.0	33.0	41.0
38-39	38.45853080727805	40.0	38.0	41.0	33.0	41.0
40-41	38.401762771324435	40.0	38.0	41.0	33.0	41.0
42-43	38.30159826676753	40.0	38.0	41.0	33.0	41.0
44-45	38.191614510451245	40.0	38.0	41.0	33.0	41.0
46-47	38.09892011324659	40.0	38.0	41.0	33.0	41.0
48-49	38.00492345543353	40.0	38.0	41.0	33.0	41.0
50-51	37.9009235993683	40.0	37.5	41.0	33.0	41.0
52-53	37.79346877165903	40.0	37.0	41.0	32.5	41.0
54-55	37.631704883228885	40.0	37.0	41.0	32.0	41.0
56-57	37.520408124903156	40.0	36.0	41.0	32.0	41.0
58-59	37.32028870737712	39.5	36.0	41.0	31.5	41.0
60-61	37.157729552467586	39.0	35.0	41.0	31.0	41.0
62-63	36.94605708678703	39.0	35.0	41.0	31.0	41.0
64-65	36.714385340780346	39.0	35.0	41.0	31.0	41.0
66-67	36.46228492694102	38.0	35.0	40.0	31.0	41.0
68-69	36.18275137765182	37.0	35.0	40.0	31.0	41.0
70-71	35.914112831436	37.0	35.0	40.0	31.0	41.0
72-73	35.59266982624978	36.0	35.0	39.0	30.0	41.0
74-75	35.264629586813214	36.0	35.0	39.0	30.0	41.0
76-77	34.93388372272255	35.5	34.5	38.5	30.0	40.0
78-79	34.62934365474559	35.0	34.0	37.0	30.0	39.0
80-81	34.316435229754546	35.0	34.0	37.0	30.0	39.0
82-83	34.030918585819364	35.0	34.0	36.5	29.0	38.5
84-85	33.779802755652724	35.0	34.0	36.0	29.0	37.0
86-87	33.55674749302413	35.0	34.0	36.0	29.0	37.0
88-89	33.38547571919722	35.0	34.0	35.0	29.0	36.5
90-91	33.20336278356963	35.0	34.0	35.0	29.0	36.0
92-93	33.094319436914276	35.0	34.0	35.0	29.0	36.0
94-95	32.98517780643942	35.0	34.0	35.0	28.5	36.0
96-97	32.90376751956076	35.0	34.0	35.0	28.0	36.0
98-99	32.82929886310326	35.0	34.0	35.0	28.0	35.0
100	31.962434903753017	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.13393864331088423
1101	2	0.15366399341293402
1101	3	0.19947915874407585
1101	4	0.23397120292693074
1101	5	0.28784269178400024
1101	6	0.2549250916795742
1101	7	0.26376195148363735
1101	8	0.37230780627628945
1101	9	0.45478948841393674
1101	10-11	0.43978112707250006
1101	12-13	0.46722569041030226
1101	14-15	0.5409038917944642
1101	16-17	0.4922813738306786
1101	18-19	0.4573854074153516
1101	20-21	0.4324873344804274
1101	22-23	0.4398589780632278
1101	24-25	0.46667058826965757
1101	26-27	0.45734582465914997
1101	28-29	0.5025984682248179
1101	30-31	0.488898471922937
1101	32-33	0.49016648564858656
1101	34-35	0.48793623954939136
1101	36-37	0.4659831043778908
1101	38-39	0.4555070132514132
1101	40-41	0.4282629606284658
1101	42-43	0.4028002042951542
1101	44-45	0.3347508087623723
1101	46-47	0.32820017856548134
1101	48-49	0.32773896995396257
1101	50-51	0.34359309975257446
1101	52-53	0.2779083284079107
1101	54-55	0.36261803938899106
1101	56-57	0.29793642204422355
1101	58-59	0.30680786322972864
1101	60-61	0.3579599217315561
1101	62-63	0.4122956290803472
1101	64-65	0.3592907339121538
1101	66-67	0.28265493450190604
1101	68-69	0.3024829121754209
1101	70-71	0.25437788750270585
1101	72-73	0.20914306472718636
1101	74-75	0.27382519064743605
1101	76-77	0.32339169381424426
1101	78-79	0.2728810647944968
1101	80-81	0.2893498333614559
1101	82-83	0.34657158060103654
1101	84-85	0.33569766593470973
1101	86-87	0.2924634699614117
1101	88-89	0.2780707604381334
1101	90-91	0.3557374946952052
1101	92-93	0.3236596970849277
1101	94-95	0.2673652296968214
1101	96-97	0.3001234894853937
1101	98-99	0.26575795448972883
1101	100	0.16499418656917797
1102	1	0.05028470263020424
1102	2	0.0997227409628465
1102	3	0.1594915422201595
1102	4	0.0927388939693472
1102	5	0.19777319196835208
1102	6	0.16928083091536905
1102	7	0.15714795055663444
1102	8	0.2892885325780412
1102	9	0.35269479226374756
1102	10-11	0.32755177122536594
1102	12-13	0.32785376172540737
1102	14-15	0.4250229874871465
1102	16-17	0.36912789785312583
1102	18-19	0.2976667856663582
1102	20-21	0.29458495220138303
1102	22-23	0.3083285342445379
1102	24-25	0.33131683382067223
1102	26-27	0.29882192984381817
1102	28-29	0.3322090582548469
1102	30-31	0.335587109391966
1102	32-33	0.3133534471799493
1102	34-35	0.320349619310619
1102	36-37	0.33800852942124493
1102	38-39	0.30956168867762557
1102	40-41	0.277607848183969
1102	42-43	0.32924836919274014
1102	44-45	0.2265810345145809
1102	46-47	0.27011320982241216
1102	48-49	0.3117667020053432
1102	50-51	0.2846441385240652
1102	52-53	0.26667973965754754
1102	54-55	0.30977403595909436
1102	56-57	0.27751290863773903
1102	58-59	0.2870683090901949
1102	60-61	0.17894044256425445
1102	62-63	0.15984138975021978
1102	64-65	0.14936190293332885
1102	66-67	0.17266443514017027
1102	68-69	0.20340316085568588
1102	70-71	0.16540139452748548
1102	72-73	0.19901287776325205
1102	74-75	0.22319652727159678
1102	76-77	0.19248881593792788
1102	78-79	0.19469469440166876
1102	80-81	0.156122287080656
1102	82-83	0.1393915437826081
1102	84-85	0.12471267052456625
1102	86-87	0.1373743574420203
1102	88-89	0.07286919326626418
1102	90-91	0.10919085958298069
1102	92-93	0.1071030135399127
1102	94-95	0.1560831249264183
1102	96-97	0.08346393308091393
1102	98-99	0.09717534421749718
1102	100	0.09541515282337443
1103	1	0.09548503468491631
1103	2	0.09092461017760911
1103	3	0.18781811310451957
1103	4	0.1403725644578131
1103	5	0.22075874723664413
1103	6	0.20765733311997536
1103	7	0.16919311210793353
1103	8	0.24368095863518846
1103	9	0.2536135707667242
1103	10-11	0.3265811531428042
1103	12-13	0.2866511890048997
1103	14-15	0.368145295478449
1103	16-17	0.31575515940425447
1103	18-19	0.15173797341270046
1103	20-21	0.2381285721957127
1103	22-23	0.2580928158508442
1103	24-25	0.25562150632995184
1103	26-27	0.2914180155378503
1103	28-29	0.3002791749136833
1103	30-31	0.2862655087756991
1103	32-33	0.3314621247474463
1103	34-35	0.3271488704287506
1103	36-37	0.35661440967401603
1103	38-39	0.29854096339838065
1103	40-41	0.2730690489627463
1103	42-43	0.2586087417693861
1103	44-45	0.2189651011647129
1103	46-47	0.17444926748174794
1103	48-49	0.1788256610630654
1103	50-51	0.2334985673565484
1103	52-53	0.24251660371033523
1103	54-55	0.17874371662025368
1103	56-57	0.22298154019595273
1103	58-59	0.2638879706687547
1103	60-61	0.20074969225778716
1103	62-63	0.12373145114867867
1103	64-65	0.1480231754696959
1103	66-67	0.2259181931799219
1103	68-69	0.17496767848817285
1103	70-71	0.1443216802915117
1103	72-73	0.1688049126712059
1103	74-75	0.12849062784830068
1103	76-77	0.1311369153292219
1103	78-79	0.11894656001096848
1103	80-81	0.1848351265881334
1103	82-83	0.1539091323056141
1103	84-85	0.1364901481398917
1103	86-87	0.1561570814514397
1103	88-89	0.1051016280468815
1103	90-91	0.18869848470586703
1103	92-93	0.11776991939854042
1103	94-95	0.12813341327584027
1103	96-97	0.12171314471478922
1103	98-99	0.07405840925132168
1103	100	0.07851650513729069
1104	1	0.03948620428927896
1104	2	0.030740003712612207
1104	3	0.03434716972979146
1104	4	-0.0046999325804790715
1104	5	0.08377771728103056
1104	6	0.05911012249232783
1104	7	-0.021121374062083476
1104	8	0.03539302147880363
1104	9	0.02608882559134429
1104	10-11	0.11466053040388857
1104	12-13	0.0767997857310121
1104	14-15	0.14424435493557297
1104	16-17	0.07214680427349407
1104	18-19	0.08931335675973884
1104	20-21	0.07561024476346745
1104	22-23	0.09832280732178589
1104	24-25	0.11145859451779216
1104	26-27	0.09874358446324294
1104	28-29	0.12329051236800126
1104	30-31	0.18532760504854906
1104	32-33	0.1315356020415379
1104	34-35	0.17659619885466782
1104	36-37	0.10773148237883134
1104	38-39	0.14453402117649716
1104	40-41	0.14066962572180586
1104	42-43	0.08060316855432603
1104	44-45	0.06684041765328885
1104	46-47	0.04942492865171744
1104	48-49	0.07968702943313843
1104	50-51	0.08430103659578236
1104	52-53	0.0491282196578382
1104	54-55	0.04680681493236705
1104	56-57	-0.0015833351439979992
1104	58-59	0.026143597923557138
1104	60-61	-0.03925249446670165
1104	62-63	-0.01425752474373354
1104	64-65	-0.028070940817130463
1104	66-67	0.002064254646150232
1104	68-69	-0.007891292025391294
1104	70-71	-0.0990669132173494
1104	72-73	-0.06980061898769918
1104	74-75	-0.06847011835258598
1104	76-77	-0.05630839561294465
1104	78-79	-0.007301841836941492
1104	80-81	4.6622700486409485E-4
1104	82-83	-0.008194639081978039
1104	84-85	-0.04844114179296355
1104	86-87	-0.08051060177680824
1104	88-89	-0.0339338337541335
1104	90-91	0.0561359368450951
1104	92-93	0.10604832210103154
1104	94-95	-0.01464457302738964
1104	96-97	-0.009209359780150805
1104	98-99	-0.07528389775139033
1104	100	-0.047765996207420613
1105	1	0.010719239148169635
1105	2	0.059537165028203276
1105	3	0.0826558636964414
1105	4	0.09225716231573244
1105	5	0.07463613046901685
1105	6	0.121309514237673
1105	7	0.050335661509315344
1105	8	0.12136915552054006
1105	9	0.17376321688679042
1105	10-11	0.20445632801143176
1105	12-13	0.21428266312751987
1105	14-15	0.3035849924896681
1105	16-17	0.2559578954808899
1105	18-19	0.1588484744531442
1105	20-21	0.21947435160790718
1105	22-23	0.1837733296698545
1105	24-25	0.1813229725177692
1105	26-27	0.21489709662909462
1105	28-29	0.23190378813136192
1105	30-31	0.19544065464560845
1105	32-33	0.20216967747889925
1105	34-35	0.1964332554169772
1105	36-37	0.21746948938861976
1105	38-39	0.18104390310335816
1105	40-41	0.19717478911513098
1105	42-43	0.19422824675174155
1105	44-45	0.1986119860334341
1105	46-47	0.08465848843601975
1105	48-49	0.10261400867800319
1105	50-51	0.1212772816618326
1105	52-53	0.09495385017074653
1105	54-55	0.1966092358725291
1105	56-57	0.11856903505744043
1105	58-59	0.18312351990761755
1105	60-61	0.13504043465677995
1105	62-63	0.1607923675718297
1105	64-65	0.16800273296841084
1105	66-67	0.14107615651407457
1105	68-69	0.17535087580372988
1105	70-71	0.1250795187081195
1105	72-73	0.1088942032224054
1105	74-75	0.09232054560029468
1105	76-77	0.08257856577041878
1105	78-79	0.03454285008735525
1105	80-81	0.08149445859793047
1105	82-83	0.13116673012847713
1105	84-85	0.13191679572901904
1105	86-87	0.08978228739012195
1105	88-89	0.0746442131389955
1105	90-91	0.16452861277821995
1105	92-93	0.16002638595282548
1105	94-95	0.10527812324087904
1105	96-97	0.09178654970575195
1105	98-99	0.03338595771263897
1105	100	0.026514000413047967
1106	1	0.07222281882220116
1106	2	-0.00236954293843894
1106	3	0.04639571422548627
1106	4	0.0711331615883779
1106	5	0.10781189710585437
1106	6	0.08725025615580506
1106	7	0.07981453342114975
1106	8	0.1152128298895505
1106	9	0.18159041723437497
1106	10-11	0.16641092656716694
1106	12-13	0.19636030147104577
1106	14-15	0.1482538891692613
1106	16-17	0.15881774985994213
1106	18-19	0.08212218114777414
1106	20-21	0.15014321615665693
1106	22-23	0.11799631285902734
1106	24-25	0.10734585635724869
1106	26-27	0.09905751353952752
1106	28-29	0.15236976064869623
1106	30-31	0.1752565875894021
1106	32-33	0.16051287990844543
1106	34-35	0.14233064487160618
1106	36-37	0.11707184892957656
1106	38-39	0.12039745976687755
1106	40-41	0.14556667260927725
1106	42-43	0.13714802443477225
1106	44-45	0.13200136232693893
1106	46-47	0.08990368796782633
1106	48-49	0.14552332251255962
1106	50-51	0.14643905190614248
1106	52-53	0.1555263286000752
1106	54-55	0.16106774735312257
1106	56-57	0.15413309335870196
1106	58-59	0.14159672072636198
1106	60-61	0.1681956219177252
1106	62-63	0.14613206802426504
1106	64-65	0.11339306650500447
1106	66-67	0.12863729218852882
1106	68-69	0.13463127509622552
1106	70-71	0.1666965614738416
1106	72-73	0.11711875613102052
1106	74-75	0.14875420586728438
1106	76-77	0.12457437212723477
1106	78-79	0.14629520562617415
1106	80-81	0.16848653720873585
1106	82-83	0.07082831988562077
1106	84-85	0.14485631823915668
1106	86-87	0.13215239669611378
1106	88-89	0.07446482780194685
1106	90-91	0.1418696180839305
1106	92-93	0.11672252745135125
1106	94-95	0.09279996616913166
1106	96-97	0.11704707607429299
1106	98-99	0.12018372294169666
1106	100	0.05745678036471347
1107	1	0.05194812124985759
1107	2	0.14547776333216333
1107	3	0.12880259314667697
1107	4	0.15442797055961677
1107	5	0.23589274649201286
1107	6	0.20612916098035328
1107	7	0.15455494002271308
1107	8	0.2047156587643002
1107	9	0.3209353034491116
1107	10-11	0.3747481510604658
1107	12-13	0.2916650362553028
1107	14-15	0.3468804775991359
1107	16-17	0.27090574970985415
1107	18-19	0.2853145436940068
1107	20-21	0.2339780308587791
1107	22-23	0.24832727859852355
1107	24-25	0.3105672443849059
1107	26-27	0.3137578899448812
1107	28-29	0.3259770949890566
1107	30-31	0.36176141367388936
1107	32-33	0.33683371530410966
1107	34-35	0.2926932154417585
1107	36-37	0.28625525949022546
1107	38-39	0.3046859285256929
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2216	34-35	-0.4904763239968446
2216	36-37	-0.483150400369162
2216	38-39	-0.4916261629171572
2216	40-41	-0.51388659431543
2216	42-43	-0.44426970790424036
2216	44-45	-0.42725736534598724
2216	46-47	-0.41361060973020614
2216	48-49	-0.4384162611510334
2216	50-51	-0.44640594946962153
2216	52-53	-0.3383890266760474
2216	54-55	-0.3851122498756183
2216	56-57	-0.44741764121705074
2216	58-59	-0.41555344828995544
2216	60-61	-0.35246709011808974
2216	62-63	-0.3696988652064519
2216	64-65	-0.4131406571461582
2216	66-67	-0.44405112658635915
2216	68-69	-0.3972854464436608
2216	70-71	-0.39346163856534844
2216	72-73	-0.3492825686412857
2216	74-75	-0.3914320745179225
2216	76-77	-0.3561353179826128
2216	78-79	-0.3889455113396636
2216	80-81	-0.3750075068279557
2216	82-83	-0.3329653290215191
2216	84-85	-0.29223181919828534
2216	86-87	-0.3476585821068454
2216	88-89	-0.3541888254814154
2216	90-91	-0.30940912011057264
2216	92-93	-0.27417922484917767
2216	94-95	-0.25502969337258463
2216	96-97	-0.26674177790928155
2216	98-99	-0.31942634067862485
2216	100	-0.3992731141996195
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	114.0
1	66.0
2	47.0
3	48.0
4	43.0
5	50.0
6	106.0
7	586.0
8	1642.0
9	2035.0
10	2129.0
11	2635.0
12	3450.0
13	4460.0
14	5520.0
15	6490.0
16	7051.0
17	7718.0
18	8494.0
19	10029.0
20	11112.0
21	12404.0
22	14104.0
23	16417.0
24	19738.0
25	25359.0
26	30037.0
27	28710.0
28	29458.0
29	38298.0
30	45165.0
31	48838.0
32	61628.0
33	84807.0
34	125704.0
35	194275.0
36	359408.0
37	825910.0
38	1216536.0
39	472064.0
40	1224.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.411621630626726	20.428513235662397	13.493212081216418	23.666653052494457
2	14.832033585226108	21.103401009039242	40.78004222860921	23.284523177125436
3	17.634346109708147	26.95177954059409	30.73256306431033	24.681311285387437
4	11.988618357799298	17.911047014400275	40.447557969029475	29.652776658770954
5	12.687910809406993	38.87274194146169	35.91252394961436	12.52682329951695
6	29.093303274921933	39.09903188388515	19.018256617510207	12.789408223682708
7	25.756603708222215	33.440088684102896	23.81371182339231	16.989595784282578
8	24.95908606294328	35.65133322770278	21.514661610722754	17.87491909863119
9	25.97950802502092	15.810032430945933	21.204349334676834	37.006110209356315
10-11	23.753859151964114	26.886123595988416	29.77594897003343	19.58406828201404
12-13	24.52164504315259	24.80788323920071	28.96759547830393	21.70287623934277
14-15	22.102199551256685	26.096656930779954	26.47123634302531	25.329907174938054
16-17	21.842033564104398	28.886528823510695	27.155846539770106	22.1155910726148
18-19	21.681816210363117	28.66222589190916	28.438383343858543	21.217574553869177
20-21	23.070053222316098	27.786719181786783	27.70397725228044	21.439250343616678
22-23	22.803261015366118	27.683089051682153	27.440608871103638	22.07304106184809
24-25	22.348995442367492	27.84165224685254	27.77302525529684	22.036327055483127
26-27	22.24949495831098	28.075618285832448	27.414824358283184	22.260062397573382
28-29	22.194595554128046	28.18037861440764	27.470417854063605	22.15460797740071
30-31	22.491754831988715	27.86691551557931	27.444146296626776	22.1971833558052
32-33	22.420442635509538	28.13057901956295	27.12661567063718	22.322362674290332
34-35	22.59075851528097	28.105164084219464	27.410389617016634	21.893687783482928
36-37	22.51960027669557	27.87104890638507	27.378129527744154	22.231221289175206
38-39	22.54226638015697	27.893306338644308	27.46861806969083	22.09580921150789
40-41	22.57327815719023	27.604155265827835	27.523620299860806	22.298946277121125
42-43	22.35541940526617	27.63387688993583	27.486536431543296	22.524167273254704
44-45	22.34838081303639	28.01411491103037	27.46933381933811	22.16817045659513
46-47	22.327459957429756	27.927980320125666	27.236280980813305	22.508278741631273
48-49	22.492454817211897	27.67404219392735	27.41329335560546	22.420209633255293
50-51	22.501866924544014	27.7981706825691	27.233943105810297	22.466019287076584
52-53	22.417164468468282	27.888352895085088	27.259911837473222	22.434570798973404
54-55	22.34111783425674	27.852694385416005	27.334863379141133	22.471324401186124
56-57	22.563274459668055	27.75358810928548	27.19582133625472	22.48731609479174
58-59	22.466787811499213	27.67523209613647	27.426920559515587	22.431059532848728
60-61	22.390156661452902	27.81030579439855	27.333652561740813	22.46588498240774
62-63	22.42145997730672	27.65919410920281	27.323495818075543	22.595850095414928
64-65	22.234197158430018	27.65308562049541	27.316693484912385	22.796023736162187
66-67	22.515121493097677	27.666073161270493	27.179708562703293	22.639096782928537
68-69	22.287085974761567	27.564435267613685	27.413995419106612	22.734483338518135
70-71	22.350701652066434	27.369400464051836	27.36467236849917	22.915225515382566
72-73	22.43424242627751	27.493227134443227	27.471615749850574	22.600914689428688
74-75	22.409586121024645	27.74998556064328	27.308947162699987	22.53148115563209
76-77	22.5171847431073	27.57736223729935	27.25358684836593	22.651866171227418
78-79	22.366030545527224	27.58994001089051	27.29356732246987	22.7504621211124
80-81	22.309263289504898	27.70721838037975	27.310100133868232	22.673418196247123
82-83	22.529708136946887	27.408974966856643	27.693772809146626	22.367544087049847
84-85	22.727846005790298	27.633694930233716	27.140223620137228	22.49823544383876
86-87	22.291001961628375	27.75569164357058	27.398125943122853	22.555180451678197
88-89	22.64362087122691	27.408699160555948	27.28312033956513	22.664559628652007
90-91	22.610329942526366	27.37413682434633	27.32018351913892	22.695349713988385
92-93	22.77891967420157	27.404278478384175	27.244323436106697	22.572478411307557
94-95	22.394140351744376	27.431486462236425	27.489082535387737	22.68529065063146
96-97	22.432713798984945	27.39776366989048	27.448654678917222	22.720867852207352
98-99	22.39433676261105	27.586483851843813	27.21484967955115	22.804329705993986
100	22.60771089567201	27.281137283511818	27.148200995155307	22.962950825660865
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1191.0
1	831.5
2	540.0
3	609.5
4	566.5
5	486.0
6	453.0
7	452.0
8	478.0
9	501.5
10	432.0
11	372.0
12	380.0
13	375.0
14	412.5
15	523.0
16	621.0
17	732.0
18	978.5
19	1234.0
20	1766.5
21	2802.0
22	4410.0
23	6817.5
24	10136.0
25	14385.0
26	19511.0
27	25265.0
28	31999.0
29	40478.5
30	48868.0
31	58223.5
32	69562.0
33	78027.0
34	85414.5
35	92719.5
36	99907.5
37	110723.5
38	117729.5
39	119169.0
40	120582.0
41	122126.5
42	124486.5
43	128676.0
44	131947.5
45	137977.0
46	144635.0
47	148762.5
48	150748.5
49	147634.5
50	141620.0
51	137287.5
52	131658.5
53	125359.0
54	119759.0
55	108897.0
56	99071.0
57	87565.0
58	75285.5
59	66393.0
60	55192.5
61	43579.5
62	33934.5
63	25530.5
64	18160.0
65	12561.0
66	9124.0
67	6489.5
68	4363.0
69	3089.5
70	2479.0
71	1944.0
72	1466.0
73	1221.5
74	1017.5
75	833.5
76	650.0
77	487.0
78	381.0
79	312.0
80	251.5
81	215.5
82	166.5
83	117.5
84	84.0
85	58.5
86	39.0
87	36.0
88	29.5
89	22.0
90	20.0
91	17.0
92	14.0
93	10.0
94	10.0
95	9.0
96	5.5
97	5.5
98	3.0
99	4.0
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06168249546377207
2	0.00985523545285344
3	0.012030369163156243
4	0.009908942458046101
5	0.012540585712486528
6	0.013211923277394802
7	0.003490955337523017
8	0.006417987120523085
9	0.008190318291880924
10-11	0.007868076260724954
12-13	0.007035617680238695
14-15	0.010727974287234194
16-17	0.010137197230114914
18-19	0.008311159053564414
20-21	0.007599541234761644
22-23	0.002671923508334924
24-25	0.004175719653729455
26-27	0.00554524828614233
28-29	0.006780509405573553
30-31	0.009788101696362612
32-33	0.011184483831371819
34-35	0.012634572971573688
36-37	0.018354369024592167
38-39	0.018998853086904112
40-41	0.01830066201939951
42-43	0.024141298834101477
44-45	0.02786050894369331
46-47	0.030102776410486937
48-49	0.03140517128640898
50-51	0.032465884638964056
52-53	0.030223617172170425
54-55	0.03658789728750085
56-57	0.03960891632958807
58-59	0.0367221648004825
60-61	0.03736664886279445
62-63	0.03278812667012003
64-65	0.03541976992456045
66-67	0.03740692911668894
68-69	0.04003857237112937
70-71	0.03974318384256973
72-73	0.03582257246350542
74-75	0.03877645774910182
76-77	0.03815882718938621
78-79	0.03759490363486326
80-81	0.04275077613335879
82-83	0.03877645774910182
84-85	0.03494983362912466
86-87	0.033204355960363155
88-89	0.030814394229289707
90-91	0.03344603748373014
92-93	0.034922980126528336
94-95	0.038481069220542174
96-97	0.033499744488922796
98-99	0.031203770016936506
100	0.028813808285863055
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3723909.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.65838311245459
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.70358670239818	36.86523376477023
2	15.293600047133395	16.412597009954748
3	6.48934753137901	10.446236879657892
4	3.2331511839282947	6.939426595508423
5	1.9321945752636471	5.183921838365162
6	1.1969241139028137	3.8535007596199446
7	0.7855803334607296	2.950707934891196
8	0.5222657522812256	2.2419148657936248
9	0.3736550885308998	1.8044755103078243
>10	1.4403751018930822	11.860706014270313
>50	0.02282530311634478	0.8115653404095946
>100	0.006402904715375486	0.5526977715697904
>500	0.0	0.0
>1k	9.136199703665317E-5	0.07701571488130245
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.37070051926618E-5
2	0.0	0.0	0.0	1.342675129816545E-4	5.37070051926618E-5
3	0.0	0.0	0.0	1.342675129816545E-4	5.37070051926618E-5
4	0.0	0.0	0.0	2.148280207706472E-4	5.37070051926618E-5
5	0.0	0.0	0.0	2.953885285596399E-4	5.37070051926618E-5
6	0.0	0.0	0.0	2.953885285596399E-4	8.05605077889927E-5
7	0.0	0.0	0.0	2.953885285596399E-4	8.05605077889927E-5
8	0.0	0.0	0.0	2.953885285596399E-4	8.05605077889927E-5
9	0.0	0.0	0.0	3.759490363486326E-4	8.05605077889927E-5
10-11	0.0	0.0	0.0	6.310573110137761E-4	1.074140103853236E-4
12-13	0.0	0.0	0.0	6.444840623119416E-4	3.222420311559708E-4
14-15	0.0	0.0	0.0	6.847643162064379E-4	3.490955337523017E-4
16-17	0.0	0.0	0.0	6.981910675046034E-4	3.6252228505046717E-4
18-19	0.0	0.0	0.0	7.653248239954307E-4	3.759490363486326E-4
20-21	0.0	0.0	0.0	7.78751575293596E-4	3.759490363486326E-4
22-23	0.0	0.0	0.0	0.0010875668551514014	4.296560415412944E-4
24-25	0.0	0.0	0.0	0.0014500891402018687	4.565095441376253E-4
26-27	0.0	0.0	0.0	0.002215413964197299	5.102165493302871E-4
28-29	0.0	0.0	0.0	0.006297146358839595	5.236433006284525E-4
30-31	0.0	0.0	0.0	0.01569587226755541	5.37070051926618E-4
32-33	0.0	0.0	0.0	0.03485584637003751	5.639235545229489E-4
34-35	0.0	0.0	0.0	0.06673095395188228	5.907770571192798E-4
36-37	0.0	0.0	0.0	0.1150269783713834	5.907770571192798E-4
38-39	0.0	0.0	0.0	0.18025413617787117	5.907770571192798E-4
40-41	0.0	0.0	0.0	0.2518590008509875	5.907770571192798E-4
42-43	0.0	0.0	0.0	0.321933215876113	5.907770571192798E-4
44-45	0.0	0.0	0.0	0.40727364712725256	6.176305597156108E-4
46-47	0.0	0.0	0.0	0.5078802946044063	6.444840623119416E-4
48-49	0.0	0.0	0.0	0.6142067381345785	6.981910675046034E-4
50-51	0.0	0.0	0.0	0.7233393726860673	7.518980726972652E-4
52-53	0.0	0.0	0.0	0.8259868863605422	8.593120830825888E-4
54-55	0.0	0.0	0.0	0.9318702470978748	8.727388343807542E-4
56-57	0.0	0.0	0.0	1.0577997475233687	8.861655856789196E-4
58-59	0.0	0.0	0.0	1.1990625979313672	9.130190882752505E-4
60-61	0.0	0.0	0.0	1.343494161645733	9.130190882752505E-4
62-63	0.0	0.0	0.0	1.4856431776394106	9.398725908715815E-4
64-65	0.0	0.0	0.0	1.6317530852660471	9.398725908715815E-4
66-67	0.0	0.0	0.0	1.7929412346005233	9.667260934679123E-4
68-69	0.0	0.0	0.0	1.9734236255504634	9.935795960642432E-4
70-71	0.0	0.0	0.0	2.1669568187622197	0.0010070063473624088
72-73	0.0	0.0	0.0	2.3558309292735133	0.0010875668551514014
74-75	0.0	0.0	0.0	2.546598211717848	0.0011547006116422287
76-77	0.0	0.0	0.0	2.7502551754084217	0.001194980865536725
78-79	0.0	0.0	0.0	2.973045259698881	0.001221834368133056
80-81	0.0	0.0	0.0	3.213021585650992	0.0012621146220275522
82-83	0.0	0.0	0.0	3.458167210852897	0.0014232356376055377
84-85	0.0	0.0	0.0	3.7028966067645586	0.001436662388903703
86-87	0.0	0.0	0.0	3.9559371617297847	0.0015440763992890267
88	0.0	0.0	0.0	4.154800775206913	0.001611210155779854
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4175	0.0	39.865154	1
GTATCAA	6930	0.0	35.482574	1
CAACGCA	7120	0.0	32.60452	5
ATCAACG	7160	0.0	32.55364	3
AACGCAG	7835	0.0	30.168926	6
TATCAAC	8105	0.0	29.975632	2
TCAACGC	8095	0.0	29.316044	4
ACGCAGA	8910	0.0	25.947466	7
GTACATG	12250	0.0	25.945215	1
GTACTAG	1740	0.0	25.399506	1
CGCAGAG	9050	0.0	25.339396	8
TACATGG	12375	0.0	24.759007	2
ACATGGG	12410	0.0	24.121178	3
CTAACGC	615	0.0	23.687403	3
CATGGGG	8590	0.0	22.210773	4
TCTAACG	690	0.0	21.112684	2
GCATCGT	1365	0.0	21.000141	8
GCAGAGT	10680	0.0	20.812052	9
TGATCGC	1035	0.0	19.977325	9
TAACGCC	670	0.0	19.638762	4
>>END_MODULE