FastQCFastQC Report
Mon 16 Jan 2017
ERR1146503_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146503_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3723909
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109820.2949051655129059No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98340.26407734453231807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56210.15094353809397598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72150.058.8401341
GTATCAA134050.042.1676751
ATCAACG163950.033.8149643
CAACGCA167200.033.2139245
TATCAAC171300.032.749512
TCAACGC174900.031.832334
AACGCAG176900.031.6162136
ACGCAGA197950.027.9914687
CGCAGAG198800.027.8008678
GTACATG113100.024.9268651
GCAGAGT220000.024.6946329
TACATGG118350.023.1043532
GAGTACT152400.022.4498712-13
ACATGGG117300.022.3688033
AGAGTAC202650.022.0431110-11
CAGAGTA214050.021.92301410-11
CATGGGG83500.021.2868734
GTACTTT166250.020.33932514-15
AGTACTT160750.020.18738712-13
TACGGGC9650.019.0039484
Produced by FastQC (version 0.11.5)