FastQCFastQC Report
Mon 16 Jan 2017
ERR1146502_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146502_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4615624
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62700.033.8179781
GTATCAA92900.031.3266241
CAACGCA101200.027.350575
ATCAACG103850.026.78843
GTACATG139050.025.6293091
AACGCAG110250.025.574056
TCAACGC112000.024.8810254
TATCAAC116900.024.601892
TACATGG143150.024.2924862
ACATGGG138600.024.106713
GTACTAG19200.023.2627491
ACGCAGA122350.022.6209077
CGCAGAG124200.022.3238
CTAACGC6900.021.7937763
CATGGGG107200.021.2607464
TGATCGC12450.020.381999
GTGATCG13150.020.0117238
ATGGGGG70500.019.1970715
GCAGAGT143200.018.7376589
TAACGCC8300.018.6838974
Produced by FastQC (version 0.11.5)