##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146502_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4615624
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40848258003685	31.0	31.0	34.0	28.0	34.0
2	31.464723296351696	31.0	31.0	34.0	28.0	34.0
3	31.65273991122327	33.0	31.0	34.0	29.0	34.0
4	35.15137931512619	37.0	35.0	37.0	32.0	37.0
5	35.20809494014244	37.0	35.0	37.0	32.0	37.0
6	35.16585536430177	37.0	35.0	37.0	32.0	37.0
7	35.15283458964595	37.0	35.0	37.0	32.0	37.0
8	35.15402034481145	37.0	35.0	37.0	32.0	37.0
9	36.76128319811146	39.0	37.0	39.0	33.0	39.0
10-11	36.735473578437066	39.0	37.0	39.0	32.5	39.0
12-13	36.690542058885214	39.0	37.0	39.0	32.0	39.0
14-15	38.08501732376814	40.0	38.0	41.0	33.0	41.0
16-17	38.09296543652603	40.0	38.0	41.0	33.0	41.0
18-19	38.114779388442386	40.0	38.0	41.0	33.0	41.0
20-21	38.213182009626436	40.0	38.5	41.0	33.0	41.0
22-23	38.23797605697518	40.0	38.0	41.0	33.0	41.0
24-25	38.29490887905948	40.0	38.0	41.0	33.0	41.0
26-27	38.32384429494257	40.0	38.0	41.0	33.0	41.0
28-29	38.28463865340851	40.0	38.0	41.0	33.0	41.0
30-31	38.26161305600283	40.0	38.0	41.0	33.0	41.0
32-33	38.26685449248032	40.0	38.0	41.0	33.0	41.0
34-35	38.20521840600534	40.0	38.0	41.0	33.0	41.0
36-37	38.11152706546287	40.0	38.0	41.0	33.0	41.0
38-39	38.07000245253946	40.0	38.0	41.0	33.0	41.0
40-41	38.00087962104365	40.0	38.0	41.0	32.0	41.0
42-43	37.86975845519479	40.0	38.0	41.0	32.0	41.0
44-45	37.717925788582434	40.0	37.5	41.0	32.0	41.0
46-47	37.61035409296771	40.0	37.0	41.0	31.5	41.0
48-49	37.49489798129137	40.0	37.0	41.0	31.0	41.0
50-51	37.37346250474475	40.0	36.5	41.0	31.0	41.0
52-53	37.25405828117715	40.0	36.0	41.0	31.0	41.0
54-55	37.077486922678276	40.0	36.0	41.0	30.5	41.0
56-57	36.950069264740804	39.0	35.0	41.0	30.0	41.0
58-59	36.73377846635688	39.0	35.0	41.0	30.0	41.0
60-61	36.55413439222952	39.0	35.0	41.0	30.0	41.0
62-63	36.32567828315305	38.0	35.0	40.0	30.0	41.0
64-65	36.07498747731618	37.5	35.0	40.0	30.0	41.0
66-67	35.81426465847305	37.0	35.0	40.0	30.0	41.0
68-69	35.52613980254891	37.0	35.0	39.5	29.0	41.0
70-71	35.25166120550547	36.0	34.0	39.0	29.0	41.0
72-73	34.9343271895631	36.0	34.0	39.0	29.0	41.0
74-75	34.617843546181405	35.0	34.0	38.5	29.0	40.0
76-77	34.30874601137354	35.0	34.0	37.0	29.0	39.5
78-79	34.02376493405875	35.0	34.0	37.0	29.0	39.0
80-81	33.74242810939539	35.0	34.0	36.0	28.0	39.0
82-83	33.48771065407408	35.0	34.0	36.0	28.0	37.0
84-85	33.26247025320953	35.0	34.0	36.0	28.0	37.0
86-87	33.067037414659424	35.0	33.5	35.0	27.5	37.0
88-89	32.920224112709356	35.0	33.0	35.0	27.0	36.0
90-91	32.758639893544185	35.0	33.0	35.0	27.0	36.0
92-93	32.670932575963725	35.0	33.0	35.0	27.0	36.0
94-95	32.575796252034394	35.0	33.0	35.0	27.0	36.0
96-97	32.49631577442183	35.0	33.0	35.0	27.0	35.0
98-99	32.42330993599132	35.0	33.0	35.0	26.0	35.0
100	31.50650031285044	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.26136813091268607
1101	2	0.24674294428984567
1101	3	0.315849687005354
1101	4	0.2606919776444343
1101	5	0.28368818814630714
1101	6	0.25963709368408416
1101	7	0.3280444775529716
1101	8	0.3235282774710555
1101	9	0.47732256859671907
1101	10-11	0.4995729778563174
1101	12-13	0.475067188524676
1101	14-15	0.6299500007930092
1101	16-17	0.5542237308008566
1101	18-19	0.5078933053595023
1101	20-21	0.49262682586075357
1101	22-23	0.49249374905342336
1101	24-25	0.48668506810583523
1101	26-27	0.5017987375093185
1101	28-29	0.571061429468763
1101	30-31	0.486871311914463
1101	32-33	0.5535393922677017
1101	34-35	0.508129727019039
1101	36-37	0.4898843037602276
1101	38-39	0.5033544367167977
1101	40-41	0.45384248238290326
1101	42-43	0.4759737486724873
1101	44-45	0.39152225287505615
1101	46-47	0.35079944373261895
1101	48-49	0.3972059133750392
1101	50-51	0.3872419833376881
1101	52-53	0.41149682228311235
1101	54-55	0.44666200625964336
1101	56-57	0.4035384062358034
1101	58-59	0.3821067668787066
1101	60-61	0.3552398281658711
1101	62-63	0.3433244294328688
1101	64-65	0.37922224877650024
1101	66-67	0.3622599901458088
1101	68-69	0.31461659095738526
1101	70-71	0.29641900163852597
1101	72-73	0.3236831468611783
1101	74-75	0.37926072409757694
1101	76-77	0.3523282887499235
1101	78-79	0.3145828633256258
1101	80-81	0.30865917998633563
1101	82-83	0.2939544232915239
1101	84-85	0.3389617658733428
1101	86-87	0.2941057000568037
1101	88-89	0.25270472784015396
1101	90-91	0.25629527094616833
1101	92-93	0.28664333444715595
1101	94-95	0.2785455201931484
1101	96-97	0.2766480409963279
1101	98-99	0.2832772328677038
1101	100	0.15262076136288982
1102	1	0.16060417632523283
1102	2	0.1965384754683086
1102	3	0.24875974072603313
1102	4	0.1672400443086346
1102	5	0.15964359951613005
1102	6	0.19049433065470112
1102	7	0.18881570100808887
1102	8	0.21449304942309766
1102	9	0.3074424070303863
1102	10-11	0.3792264043741511
1102	12-13	0.3640677064527793
1102	14-15	0.3603522100521346
1102	16-17	0.33384574968203395
1102	18-19	0.3180272474392254
1102	20-21	0.3248908525836356
1102	22-23	0.32043769365682806
1102	24-25	0.34618632504967195
1102	26-27	0.36433627878503216
1102	28-29	0.3371985072052155
1102	30-31	0.3043700043268842
1102	32-33	0.36336274771831256
1102	34-35	0.3230969128479728
1102	36-37	0.34733521230484854
1102	38-39	0.29787937918931817
1102	40-41	0.2630217684932816
1102	42-43	0.23847613722750793
1102	44-45	0.1616785292741767
1102	46-47	0.15885920506011075
1102	48-49	0.20206843687224563
1102	50-51	0.17651678611432686
1102	52-53	0.2235135202510179
1102	54-55	0.18436461849832142
1102	56-57	0.19065425317592855
1102	58-59	0.0972786130391654
1102	60-61	0.1010070107604264
1102	62-63	0.14532766637710637
1102	64-65	0.08807967044785414
1102	66-67	0.022170695425991482
1102	68-69	0.05808973934314565
1102	70-71	0.062236300266846456
1102	72-73	0.0793510123803216
1102	74-75	0.09837703747089677
1102	76-77	0.09255887527690021
1102	78-79	0.06433392163106078
1102	80-81	0.0679572235629422
1102	82-83	0.12050800544313489
1102	84-85	0.16723629925927241
1102	86-87	0.18612692617178794
1102	88-89	0.10216205239466802
1102	90-91	0.1727958559027627
1102	92-93	0.12013472735031172
1102	94-95	0.1208712258967779
1102	96-97	0.09199313985164537
1102	98-99	0.13684661714609092
1102	100	0.04253554793352876
1103	1	0.09997928276027679
1103	2	0.11607197585075468
1103	3	0.20789612487878983
1103	4	0.10513719393799903
1103	5	0.13137817237485905
1103	6	0.08734750618598497
1103	7	0.1059837433186317
1103	8	0.1744781235729178
1103	9	0.20119423238610779
1103	10-11	0.22193456172501413
1103	12-13	0.19009826402781727
1103	14-15	0.2490576402983109
1103	16-17	0.24338293840339986
1103	18-19	0.20596664131712572
1103	20-21	0.20774241884241462
1103	22-23	0.1967260121750769
1103	24-25	0.22331149570511144
1103	26-27	0.19233183654355912
1103	28-29	0.21266692709274793
1103	30-31	0.1834516772735313
1103	32-33	0.21043873713932015
1103	34-35	0.25751254788742983
1103	36-37	0.24156702060052027
1103	38-39	0.23120026585855413
1103	40-41	0.21572364163167634
1103	42-43	0.16680216575573326
1103	44-45	0.14039037902264795
1103	46-47	0.06408644039050415
1103	48-49	0.1054312092719627
1103	50-51	0.12664603863296264
1103	52-53	0.12620758040189628
1103	54-55	0.1692347766233766
1103	56-57	0.13844772682848827
1103	58-59	0.1842858684634905
1103	60-61	0.0962675967243456
1103	62-63	0.09196305910909786
1103	64-65	0.09434671802303285
1103	66-67	0.08805853238010286
1103	68-69	0.09953002807869638
1103	70-71	0.10293460885651484
1103	72-73	0.049947628680271805
1103	74-75	0.057782521545505006
1103	76-77	0.03129034035919176
1103	78-79	0.0659635298773793
1103	80-81	0.09276324754543452
1103	82-83	0.06449302818281666
1103	84-85	0.11128647250635737
1103	86-87	0.12302821583897128
1103	88-89	0.0794703970204722
1103	90-91	0.14763778242313208
1103	92-93	0.0968730367449595
1103	94-95	0.12316941955774041
1103	96-97	0.08943891019276151
1103	98-99	0.0269871342339556
1103	100	0.0643004679916146
1104	1	0.14599874606802032
1104	2	0.11834094077406121
1104	3	0.18755390621481283
1104	4	0.18881772180552758
1104	5	0.13699888560418572
1104	6	0.1201804349640554
1104	7	0.12664418376819953
1104	8	0.1478051201142634
1104	9	0.27411635470107143
1104	10-11	0.24930875886091997
1104	12-13	0.17709545393700665
1104	14-15	0.23588790464673082
1104	16-17	0.22106826033050453
1104	18-19	0.15316505626854848
1104	20-21	0.16963963947867455
1104	22-23	0.1632222978155511
1104	24-25	0.17275577712246104
1104	26-27	0.1903893599423725
1104	28-29	0.2320103301839609
1104	30-31	0.23451755545615072
1104	32-33	0.20373197112536445
1104	34-35	0.17023543869791524
1104	36-37	0.21437646816196576
1104	38-39	0.22287854820516628
1104	40-41	0.18944769773142411
1104	42-43	0.18317160923442088
1104	44-45	0.18087208685188472
1104	46-47	0.13311175459863733
1104	48-49	0.12444227633032057
1104	50-51	0.1328883900931359
1104	52-53	0.1130727715316624
1104	54-55	0.16842789886499077
1104	56-57	0.10976909149278669
1104	58-59	0.08477885705832477
1104	60-61	0.08157711073705087
1104	62-63	0.059653899148692346
1104	64-65	0.03284445708541739
1104	66-67	0.016621409623901684
1104	68-69	-0.009710152285961726
1104	70-71	-0.010297378333802953
1104	72-73	0.015991966023008786
1104	74-75	-0.04083951858254409
1104	76-77	0.007009848003214358
1104	78-79	0.059528788734269256
1104	80-81	0.015052142530116441
1104	82-83	0.005211222749977651
1104	84-85	-0.015712301600828482
1104	86-87	0.08118271554605627
1104	88-89	0.03673258923153355
1104	90-91	0.07300956392289493
1104	92-93	0.03424650025896625
1104	94-95	0.001184472628374067
1104	96-97	-0.05222661351225355
1104	98-99	-0.03181749800351241
1104	100	-0.08345549959718213
1105	1	0.1586453583513645
1105	2	0.16922788313276627
1105	3	0.23147484123229134
1105	4	0.19728166570144623
1105	5	0.20896809210381662
1105	6	0.17789882554785663
1105	7	0.18550355217986692
1105	8	0.2419924698498619
1105	9	0.25067674480393976
1105	10-11	0.315770622473849
1105	12-13	0.30404130824036457
1105	14-15	0.3456579067383956
1105	16-17	0.34940347189471765
1105	18-19	0.34703291703472416
1105	20-21	0.3013713066884094
1105	22-23	0.2642561311092777
1105	24-25	0.3349217449070139
1105	26-27	0.3324268586321182
1105	28-29	0.34539646030267335
1105	30-31	0.36364505081155585
1105	32-33	0.2819869310066423
1105	34-35	0.274660960914872
1105	36-37	0.3101054494066986
1105	38-39	0.20605804209783685
1105	40-41	0.27480873781599513
1105	42-43	0.20774581761465072
1105	44-45	0.18657252942188762
1105	46-47	0.22014297354676415
1105	48-49	0.13573504461971453
1105	50-51	0.1516633353469743
1105	52-53	0.1626516162014937
1105	54-55	0.2308320960469743
1105	56-57	0.19469625313021055
1105	58-59	0.15358657649236562
1105	60-61	0.15368786043100613
1105	62-63	0.18253600411429005
1105	64-65	0.1423145232551164
1105	66-67	0.1823554587718874
1105	68-69	0.15893076744006152
1105	70-71	0.19363666161275006
1105	72-73	0.20561721864743276
1105	74-75	0.1787447022860178
1105	76-77	0.14417133364589318
1105	78-79	0.1366929518045481
1105	80-81	0.15425939392420673
1105	82-83	0.11866872327052391
1105	84-85	0.1409886664943798
1105	86-87	0.161502211629994
1105	88-89	0.15845545579129805
1105	90-91	0.13823978712406415
1105	92-93	0.09125998510291566
1105	94-95	0.07555176731663238
1105	96-97	0.02443560895660113
1105	98-99	0.08412253995003027
1105	100	0.006658436923355993
1106	1	0.09777697518516959
1106	2	0.1529537022382783
1106	3	0.10523257930210406
1106	4	0.18710966740180623
1106	5	0.14015392944694582
1106	6	0.13685787365277235
1106	7	0.14983020969996375
1106	8	0.19774894276669386
1106	9	0.19560766600061186
1106	10-11	0.20510503048872408
1106	12-13	0.2581222123913278
1106	14-15	0.26455112802401715
1106	16-17	0.21161280483367761
1106	18-19	0.23859553633123198
1106	20-21	0.18842086346846543
1106	22-23	0.2274076886990315
1106	24-25	0.23941846690889435
1106	26-27	0.2584812841198669
1106	28-29	0.3429118356992973
1106	30-31	0.32570424086112837
1106	32-33	0.2673778527997115
1106	34-35	0.29127376141892114
1106	36-37	0.25647645820411213
1106	38-39	0.23326464064790997
1106	40-41	0.2448765150197616
1106	42-43	0.23552443491742991
1106	44-45	0.19656769017593234
1106	46-47	0.16193388095821604
1106	48-49	0.1643994951194898
1106	50-51	0.2771376333898985
1106	52-53	0.23863896028379372
1106	54-55	0.2659135398222219
1106	56-57	0.21358069634245425
1106	58-59	0.22233858071838597
1106	60-61	0.1901975153266875
1106	62-63	0.19926033831275447
1106	64-65	0.20003439664310463
1106	66-67	0.18459012879915804
1106	68-69	0.20659010906082642
1106	70-71	0.16948342667443228
1106	72-73	0.17823435342876337
1106	74-75	0.12304896844311486
1106	76-77	0.2013181808511817
1106	78-79	0.16834139876389287
1106	80-81	0.19984583638800046
1106	82-83	0.18001779606328938
1106	84-85	0.24303248226668472
1106	86-87	0.20210121517874313
1106	88-89	0.20248047130795754
1106	90-91	0.20793172353795342
1106	92-93	0.17206775859396828
1106	94-95	0.10578180880752086
1106	96-97	0.15559932313698255
1106	98-99	0.0741070514973714
1106	100	0.06964233353542681
1107	1	0.04350296223300276
1107	2	0.09876376520969998
1107	3	0.11308992829218312
1107	4	0.15766502462141574
1107	5	0.16151443314812752
1107	6	0.115964502274025
1107	7	0.0705654727299816
1107	8	0.0795422730065738
1107	9	0.16406678282184828
1107	10-11	0.21663731309961065
1107	12-13	0.20831551684265293
1107	14-15	0.21810126350160175
1107	16-17	0.22899360517331502
1107	18-19	0.1811274057990886
1107	20-21	0.17743803478072095
1107	22-23	0.24051215160869788
1107	24-25	0.24638407165468834
1107	26-27	0.1916441045798365
1107	28-29	0.19099982113139902
1107	30-31	0.18690741250144072
1107	32-33	0.19339981165908426
1107	34-35	0.19771170970324192
1107	36-37	0.19826688503963652
1107	38-39	0.20837029474280655
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2216	40-41	-0.4064839894218508
2216	42-43	-0.3946539101825266
2216	44-45	-0.29249079795177124
2216	46-47	-0.26694807353145933
2216	48-49	-0.22797804347822392
2216	50-51	-0.29139911126659257
2216	52-53	-0.1740913751016535
2216	54-55	-0.22599720719396998
2216	56-57	-0.22789271640451858
2216	58-59	-0.21495191635623456
2216	60-61	-0.23621088012556157
2216	62-63	-0.2140951490401477
2216	64-65	-0.25085781638352245
2216	66-67	-0.22401820747607104
2216	68-69	-0.2509752452915137
2216	70-71	-0.25785530284157687
2216	72-73	-0.22240953511916217
2216	74-75	-0.24492142873157263
2216	76-77	-0.20524404082524228
2216	78-79	-0.23040648810680864
2216	80-81	-0.1920048573611055
2216	82-83	-0.15774428687691255
2216	84-85	-0.17726456963085724
2216	86-87	-0.1736280100059986
2216	88-89	-0.20638445634662617
2216	90-91	-0.19510204273434795
2216	92-93	-0.1828589060284358
2216	94-95	-0.19010781498175788
2216	96-97	-0.17222008680715106
2216	98-99	-0.1926627063141808
2216	100	-0.21564847251135788
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	107.0
1	69.0
2	59.0
3	64.0
4	47.0
5	72.0
6	193.0
7	798.0
8	2489.0
9	3872.0
10	4463.0
11	5629.0
12	6965.0
13	8232.0
14	9600.0
15	10567.0
16	11227.0
17	12270.0
18	13497.0
19	14964.0
20	16936.0
21	18948.0
22	21319.0
23	24609.0
24	29291.0
25	35679.0
26	41136.0
27	40558.0
28	43712.0
29	54880.0
30	63515.0
31	69461.0
32	88518.0
33	119558.0
34	177430.0
35	274509.0
36	510960.0
37	1093851.0
38	1363311.0
39	421508.0
40	751.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.70334238469486	19.688120402329623	13.222083819105151	24.386453393870365
2	15.065039688575663	20.903682671669284	39.863585011382	24.167692628373054
3	17.911603929022434	26.03512562696475	30.58502409052734	25.46824635348548
4	12.195635631193676	17.74686730259543	39.585828715251715	30.47166835095918
5	13.13848352659848	38.34790586330539	35.3730036081746	13.140607001921529
6	29.692438967524755	38.36673395327149	18.597126292825873	13.343700786377886
7	26.332592134714854	33.09424748840699	23.138743238268813	17.434417138609344
8	25.70458277532716	34.748171931695495	21.195799012478034	18.35144628049931
9	26.31811583212359	15.69150434424631	20.743873637683357	37.24650618594674
10-11	24.192700230796365	26.38591570169288	29.117381574944684	20.30400249256607
12-13	25.028535139822182	24.42195216756562	28.264521829490295	22.284990863121894
14-15	22.50327372997857	25.72644195522636	25.91386821030786	25.856416104487213
16-17	22.29244945535133	28.34649678658045	26.693829944116988	22.66722381395123
18-19	22.139157471656617	28.19475545877548	27.807790435022834	21.85829663454507
20-21	23.29411642351056	27.375561811781584	27.19198814988408	22.138333614823782
22-23	23.223137469778525	27.247027359279336	26.921526964848297	22.608308206093845
24-25	22.887249446615108	27.46016457519876	27.13917804699143	22.513407931194703
26-27	22.73238456130985	27.612260203845622	26.857125957215665	22.798229277628863
28-29	22.588632179232246	27.864209721792648	26.947486676261455	22.599671422713648
30-31	22.899959926031624	27.493515741572633	26.911397745132376	22.695126587263367
32-33	22.79121583681038	27.734601707659767	26.503600698266023	22.970581757263826
34-35	23.199800220364306	27.745220293884078	26.728413452839977	22.32656603291164
36-37	23.164487057290604	27.35768848760813	26.757594810326257	22.72022964477501
38-39	22.94966905068633	27.44883531195047	26.84410114946514	22.757394487898058
40-41	23.006780402923656	27.12876264689248	27.077730851158343	22.78672609902552
42-43	22.803793912554944	27.227764236517626	26.9093438668024	23.05909798412503
44-45	22.80556079586255	27.532943772342716	26.9215669978336	22.739928433961136
46-47	22.954703242244392	27.45350710460356	26.586221981083934	23.005567672068114
48-49	22.982907685202502	27.313548087319546	26.701142283383454	23.002401944094498
50-51	22.963153439903376	27.397676949495665	26.61735962767561	23.021809982925344
52-53	22.910629394771835	27.45829006580421	26.6516420928051	22.979438446618857
54-55	22.702122674684105	27.41090429595684	26.70195535575537	23.185017673603685
56-57	22.97916190968705	27.399812455344897	26.6046062120347	23.016419422933353
58-59	22.892165548047526	27.2499958007758	26.812355363818074	23.0454832873586
60-61	22.727586993870936	27.51142900923445	26.812716830406792	22.948267166487824
62-63	22.8561372467471	27.273053347414766	26.756518533464615	23.11429087237352
64-65	22.54231899235458	27.36983973139165	26.70896037367978	23.37888090257399
66-67	22.824810573221903	27.28995084440129	26.649681832368138	23.23555675000867
68-69	22.80084767115301	27.2290895771235	26.812861583710234	23.157201168013255
70-71	22.722793602491638	27.114537767179204	26.784648970946574	23.378019659382584
72-73	22.932329401198377	27.115866406747045	26.805484319917866	23.14631987213671
74-75	22.85514577752347	27.46530035458226	26.692154975855466	22.987398892038804
76-77	23.00975208528171	27.15021072263396	26.647402351814144	23.192634840270184
78-79	22.815223080615922	27.19187501286868	26.69284111215746	23.30006079435794
80-81	22.939022459352227	27.271638615550824	26.83292258117444	22.956416343922506
82-83	23.17639318736987	27.011036176451814	27.07546146441226	22.737109171766054
84-85	23.219178980286326	27.257156189149594	26.63108750581514	22.892577324748935
86-87	22.951554767921003	27.44567355215983	26.700218916073936	22.902552763845232
88-89	23.1767712587075	27.01721155195459	26.60537395722658	23.200643232111325
90-91	23.051016651923785	27.00773272484952	26.832833722521542	23.108416900705155
92-93	23.249787333185235	27.062044527283664	26.85141497932933	22.836753160201777
94-95	22.78304207512533	27.06607656446079	27.02936134151443	23.121520018899453
96-97	22.89189814476179	26.96229327620407	26.959562514662945	23.1862460643712
98-99	22.817979527295403	27.222444663936322	26.706363031017887	23.253212777750388
100	23.055841620549522	26.828128684170377	26.761726931025397	23.354302764254705
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1505.0
1	1153.0
2	853.0
3	914.5
4	863.0
5	783.5
6	737.5
7	699.5
8	744.0
9	773.5
10	655.5
11	555.5
12	572.5
13	611.0
14	658.0
15	772.5
16	916.0
17	1060.0
18	1285.5
19	1600.5
20	2120.0
21	3024.0
22	4591.5
23	6585.5
24	9107.0
25	13145.0
26	19021.0
27	25978.0
28	33765.5
29	42133.0
30	52679.0
31	65198.5
32	78575.5
33	87400.0
34	93346.0
35	101395.0
36	111018.0
37	124862.0
38	131291.0
39	131204.0
40	134258.5
41	137646.0
42	140757.5
43	147128.0
44	155358.5
45	167568.5
46	179412.0
47	184850.5
48	188617.5
49	185175.5
50	177790.5
51	176580.5
52	175779.0
53	174870.5
54	172130.5
55	158717.0
56	142811.0
57	125559.0
58	108096.0
59	93943.5
60	77469.5
61	62397.5
62	51462.0
63	39538.5
64	27540.5
65	19160.0
66	13508.5
67	9797.5
68	7638.5
69	6065.5
70	4703.5
71	3507.0
72	2684.0
73	2293.5
74	1853.5
75	1431.5
76	1093.5
77	749.5
78	592.5
79	458.0
80	275.0
81	184.0
82	156.5
83	123.5
84	93.0
85	72.0
86	62.5
87	51.0
88	40.0
89	31.0
90	18.0
91	14.5
92	15.0
93	12.5
94	11.0
95	9.5
96	8.0
97	10.0
98	10.0
99	12.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05828031052789395
2	0.009576169982650234
3	0.011526068847895756
4	0.009359514553178508
5	0.011872717535050516
6	0.011699393191473137
7	0.002751523954290904
8	0.005048071506691186
9	0.007019635914883882
10-11	0.006499662884151742
12-13	0.0056763722521591875
14-15	0.01030196567138051
16-17	0.009684497697386095
18-19	0.007561274488563193
20-21	0.006727151085097053
22-23	0.0024915374389248342
24-25	0.0035856473577570444
26-27	0.004755586676904358
28-29	0.0058821949101573266
30-31	0.008850374293919955
32-33	0.010345296757274856
34-35	0.011677727648525964
36-37	0.018296551018887153
38-39	0.018827356821092878
40-41	0.01850237367688529
42-43	0.024406234129989792
44-45	0.02782938991564304
46-47	0.03027759626867353
48-49	0.03130670955866422
50-51	0.031967508618552985
52-53	0.03045092061225091
54-55	0.03641977769419693
56-57	0.03877048910396514
58-59	0.03632228275093465
60-61	0.03698308181082341
62-63	0.03273663539317761
64-65	0.03509817957441941
66-67	0.03691808518198189
68-69	0.03913880333406707
70-71	0.03892214790459535
72-73	0.03539066440420623
74-75	0.03778470689986879
76-77	0.03767637918513293
78-79	0.03702641289671776
80-81	0.041890327288357974
82-83	0.03796886401491976
84-85	0.03452404268631934
86-87	0.03308328408033236
88-89	0.030905897014141537
90-91	0.03320244456654181
92-93	0.03464320317252879
94-95	0.03756805147039707
96-97	0.03307245130885878
98-99	0.031014224728877396
100	0.027905219315958144
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4615624.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.32281772171057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.26109997711785	37.62458109262145
2	15.172327876088124	16.484072032535266
3	6.25265169388958	10.189849747335254
4	3.21613167473322	6.988373389422097
5	1.887797354607151	5.127523579492585
6	1.1206789944327107	3.652706444347083
7	0.783690792835273	2.980060446256137
8	0.5369188234553643	2.333355470233685
9	0.35221765846773917	1.722011009337963
>10	1.3882407322240662	11.593067782029898
>50	0.023377818664537454	0.8358915302440463
>100	0.004794283758049826	0.394335294622075
>500	0.0	0.0
>1k	7.231972629949895E-5	0.07417218152244771
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9498988652455227E-4
2	0.0	0.0	0.0	0.0	1.9498988652455227E-4
3	0.0	0.0	0.0	0.0	1.9498988652455227E-4
4	0.0	0.0	0.0	0.0	1.9498988652455227E-4
5	0.0	0.0	0.0	0.0	2.1665542947172474E-4
6	0.0	0.0	0.0	0.0	2.5998651536606967E-4
7	0.0	0.0	0.0	0.0	2.8165205831324217E-4
8	0.0	0.0	0.0	0.0	2.8165205831324217E-4
9	0.0	0.0	0.0	0.0	2.8165205831324217E-4
10-11	0.0	0.0	0.0	0.0	3.249831442075871E-4
12-13	0.0	0.0	0.0	0.0	0.001061611604411451
14-15	0.0	0.0	0.0	2.1665542947172475E-5	0.0012890998053567623
16-17	0.0	0.0	0.0	4.333108589434495E-5	0.0015707518636700044
18-19	0.0	0.0	0.0	5.4163857367931184E-5	0.0017332434357737979
20-21	0.0	0.0	0.0	1.4082602915662108E-4	0.0020040627226134535
22-23	0.0	0.0	0.0	5.091402592585532E-4	0.0021340559802964887
24-25	0.0	0.0	0.0	0.001050778832937865	0.0021448887517700748
26-27	0.0	0.0	0.0	0.0017982400646153153	0.0023723769527153856
28-29	0.0	0.0	0.0	0.0056872050236327736	0.002437373581556903
30-31	0.0	0.0	0.0	0.014895060776181076	0.0024915374389248342
32-33	0.0	0.0	0.0	0.03327827396685692	0.002567366839239938
34-35	0.0	0.0	0.0	0.062320934287541624	0.0027298584113437316
36-37	0.0	0.0	0.0	0.10878269113775299	0.0027298584113437316
38-39	0.0	0.0	0.0	0.17389848046547984	0.0028598516690267663
40-41	0.0	0.0	0.0	0.24042253008477293	0.002881517211973939
42-43	0.0	0.0	0.0	0.3042817179215638	0.0029465138408154563
44-45	0.0	0.0	0.0	0.3838809227094755	0.0030981726414456637
46-47	0.0	0.0	0.0	0.4794367998779797	0.0033148280709173884
48-49	0.0	0.0	0.0	0.5780583513735088	0.0034664868715475958
50-51	0.0	0.0	0.0	0.6799080687681666	0.0037264733869136653
52-53	0.0	0.0	0.0	0.7779338178326485	0.004073122074068425
54-55	0.0	0.0	0.0	0.8814409492627648	0.004159784245857115
56-57	0.0	0.0	0.0	1.0035046182271348	0.004300610275013736
58-59	0.0	0.0	0.0	1.1387301045319116	0.004408937989749598
60-61	0.0	0.0	0.0	1.2763604661038248	0.004517265704485461
62-63	0.0	0.0	0.0	1.412495905212383	0.0048314160772194616
64-65	0.0	0.0	0.0	1.551621189247651	0.004961409334902496
66-67	0.0	0.0	0.0	1.7045257585973208	0.0050047404207968415
68-69	0.0	0.0	0.0	1.874535274103783	0.0050047404207968415
70-71	0.0	0.0	0.0	2.05615752062993	0.0050372387352176
72-73	0.0	0.0	0.0	2.2401846424232126	0.005329723565004428
74-75	0.0	0.0	0.0	2.4192503548815933	0.005708870566579947
76-77	0.0	0.0	0.0	2.6075997524928374	0.005914693224578086
78-79	0.0	0.0	0.0	2.8169863923057856	0.005968857081946017
80-81	0.0	0.0	0.0	3.0497393201872596	0.006023020939313948
82-83	0.0	0.0	0.0	3.288753589980466	0.0064130007123630525
84-85	0.0	1.0832771473586237E-5	0.0	3.5254604794497992	0.006477997341204569
86-87	0.0	2.1665542947172475E-5	0.0	3.767399597540874	0.006867977114253674
88	0.0	2.1665542947172475E-5	0.0	3.9566047840985314	0.007236291344355606
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6270	0.0	33.817978	1
GTATCAA	9290	0.0	31.326624	1
CAACGCA	10120	0.0	27.35057	5
ATCAACG	10385	0.0	26.7884	3
GTACATG	13905	0.0	25.629309	1
AACGCAG	11025	0.0	25.57405	6
TCAACGC	11200	0.0	24.881025	4
TATCAAC	11690	0.0	24.60189	2
TACATGG	14315	0.0	24.292486	2
ACATGGG	13860	0.0	24.10671	3
GTACTAG	1920	0.0	23.262749	1
ACGCAGA	12235	0.0	22.620907	7
CGCAGAG	12420	0.0	22.323	8
CTAACGC	690	0.0	21.793776	3
CATGGGG	10720	0.0	21.260746	4
TGATCGC	1245	0.0	20.38199	9
GTGATCG	1315	0.0	20.011723	8
ATGGGGG	7050	0.0	19.197071	5
GCAGAGT	14320	0.0	18.737658	9
TAACGCC	830	0.0	18.683897	4
>>END_MODULE