FastQCFastQC Report
Mon 16 Jan 2017
ERR1146502_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146502_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4615624
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158840.3441354841728876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142150.3079756929940567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78800.1707244784237191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108450.053.1202741
GTATCAA190200.040.830231
ATCAACG233700.032.6927153
CAACGCA234900.032.4856725
TATCAAC243150.031.8001632
TCAACGC244750.031.21674
AACGCAG247400.031.0589086
ACGCAGA276300.027.6058447
CGCAGAG279250.027.2805568
GCAGAGT307350.024.6334789
GAGTACT204750.022.95300312-13
GTACATG137650.021.9858471
CAGAGTA300500.021.5041210-11
AGAGTAC279250.021.17987610-11
GTACTTT221150.021.02772514-15
CTAACGC7900.020.8303853
TACATGG142350.020.8280752
AGTACTT210850.020.71758812-13
ACATGGG138600.020.3537463
GTGATCG10650.020.298868
Produced by FastQC (version 0.11.5)