FastQCFastQC Report
Mon 16 Jan 2017
ERR1146501_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146501_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2395044
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30600.040.7110441
GTATCAA47600.033.1833421
CAACGCA49550.030.250065
ATCAACG50500.029.867713
AACGCAG54350.028.0954386
TATCAAC57750.026.7690032
TCAACGC58700.026.015584
TGATCGC6550.025.1076419
GTACTAG9650.024.8445221
ACGCAGA60700.024.613957
GTACATG83500.024.4901031
CGCAGAG62550.023.7377178
GTGATCG6950.023.6625988
CTAACGC6000.022.7109363
TACATGG87600.022.5822012
CTGTGCG13250.022.3410649
TAACGCC5700.021.4331874
ACATGGG86600.021.4322363
GTATAAG14050.021.0790981
CATGGGG62350.020.5737974
Produced by FastQC (version 0.11.5)