FastQCFastQC Report
Mon 16 Jan 2017
ERR1146501_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146501_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2395044
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93990.3924353790577543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84220.3516428090673908No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48570.2027937691332602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56500.059.4622041
GTATCAA101200.041.752941
ATCAACG127500.032.7794573
CAACGCA129300.032.3224565
TATCAAC132200.031.8533862
AACGCAG135350.031.215886
TCAACGC137350.030.5991884
ACGCAGA148850.028.005867
GTACTAG11500.027.8228931
CGCAGAG151000.027.6071018
GTACATG81550.025.849091
CGACGAG5300.025.71557624-25
GCAGAGT164950.025.0444019
TACATGG84600.024.1370052
GTGGTAT17650.023.9932481
GAGTACT116900.023.8404412-13
ACATGGG82650.023.6056183
CATGGGG57200.023.3412114
CAGAGTA162050.022.5779110-11
AGTACTT121850.022.3126912-13
Produced by FastQC (version 0.11.5)