FastQCFastQC Report
Mon 16 Jan 2017
ERR1146500_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146500_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3189289
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72300.026.0124841
CTAACGC8700.025.9256743
GTATCAA99800.024.6394651
GTACATG113050.024.5381371
TAACGCC9200.024.516674
TACATGG110250.024.2090532
TCTAACG9400.023.9950372
CGCCTAC9400.023.4918257
GTACTAG15200.023.1994551
ACATGGG113150.022.6334113
CAACGCA103850.022.4431235
ATCAACG104750.022.2951533
GCTATTG23700.022.0070748
GCATCGT14300.021.6868048
CTGTGCG20150.021.2204199
TATCAAC114600.021.1989352
GGGCTAT26100.021.063626
AACGCAG113800.021.0166366
CATGGGG79450.020.8779984
ACGCCTA10450.020.6837466
Produced by FastQC (version 0.11.5)