##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146500_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3189289
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.347464278088314	31.0	31.0	34.0	29.0	34.0
2	31.55975046475876	31.0	31.0	34.0	30.0	34.0
3	31.741902975867035	33.0	31.0	34.0	30.0	34.0
4	35.29955830280667	37.0	35.0	37.0	33.0	37.0
5	35.40511913470369	37.0	35.0	37.0	33.0	37.0
6	35.387511448476445	37.0	35.0	37.0	33.0	37.0
7	35.306530076139225	37.0	35.0	37.0	33.0	37.0
8	35.29657519277808	37.0	35.0	37.0	33.0	37.0
9	36.93653068128978	39.0	38.0	39.0	33.0	39.0
10-11	36.91906205426977	39.0	38.0	39.0	33.0	39.0
12-13	36.879850806872625	39.0	38.0	39.0	33.0	39.0
14-15	38.31757172209856	41.0	39.0	41.0	34.0	41.0
16-17	38.34484002547276	41.0	39.0	41.0	33.5	41.0
18-19	38.38860542271334	41.0	39.0	41.0	33.5	41.0
20-21	38.48319954698367	41.0	39.0	41.0	34.0	41.0
22-23	38.524115406286484	41.0	39.0	41.0	34.0	41.0
24-25	38.585844525221766	41.0	39.0	41.0	34.0	41.0
26-27	38.612104610149785	41.0	39.0	41.0	34.0	41.0
28-29	38.579728585274026	41.0	39.0	41.0	34.0	41.0
30-31	38.56491603614474	41.0	39.0	41.0	34.0	41.0
32-33	38.56899202298694	40.0	39.0	41.0	33.5	41.0
34-35	38.5102858348679	40.0	38.5	41.0	33.0	41.0
36-37	38.42279736957046	40.0	38.0	41.0	33.0	41.0
38-39	38.37853452603386	40.0	38.0	41.0	33.0	41.0
40-41	38.3071565480582	40.0	38.0	41.0	33.0	41.0
42-43	38.196752473670465	40.0	38.0	41.0	33.0	41.0
44-45	38.075350963804155	40.0	38.0	41.0	33.0	41.0
46-47	37.972546232091226	40.0	38.0	41.0	33.0	41.0
48-49	37.85567896167453	40.0	37.0	41.0	32.5	41.0
50-51	37.725936878094146	40.0	37.0	41.0	32.0	41.0
52-53	37.59226915466112	40.0	36.5	41.0	32.0	41.0
54-55	37.4147275458574	40.0	36.0	41.0	32.0	41.0
56-57	37.277812233384935	39.5	35.5	41.0	31.0	41.0
58-59	37.051063105287724	39.0	35.0	41.0	31.0	41.0
60-61	36.8667828472114	39.0	35.0	41.0	31.0	41.0
62-63	36.62434370168398	39.0	35.0	41.0	31.0	41.0
64-65	36.37106828512562	38.0	35.0	40.5	31.0	41.0
66-67	36.10511151545062	37.0	35.0	40.0	30.0	41.0
68-69	35.813728545766786	37.0	35.0	40.0	30.0	41.0
70-71	35.55569752380546	36.0	35.0	39.0	30.0	41.0
72-73	35.237235007551845	36.0	35.0	39.0	30.0	41.0
74-75	34.911805264433546	35.0	34.5	39.0	30.0	40.5
76-77	34.59286395807969	35.0	34.0	37.0	29.5	39.5
78-79	34.30399299028718	35.0	34.0	37.0	29.0	39.0
80-81	34.0170172725018	35.0	34.0	36.5	29.0	39.0
82-83	33.747445120213314	35.0	34.0	36.0	29.0	37.5
84-85	33.51349752248855	35.0	34.0	36.0	29.0	37.0
86-87	33.298240610995116	35.0	34.0	35.5	28.5	37.0
88-89	33.141529820596375	35.0	34.0	35.0	28.0	36.0
90-91	32.9583943317774	35.0	34.0	35.0	28.0	36.0
92-93	32.84903484757888	35.0	34.0	35.0	28.0	36.0
94-95	32.7392638296498	35.0	34.0	35.0	27.0	36.0
96-97	32.64728972507666	35.0	34.0	35.0	27.0	35.5
98-99	32.55979216684345	35.0	33.5	35.0	27.0	35.0
100	31.664245228325186	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2888560566130316
1101	2	0.303786011920959
1101	3	0.431014379149147
1101	4	0.3694109663863685
1101	5	0.37324188006063963
1101	6	0.41031380212478297
1101	7	0.4320373027208433
1101	8	0.45966116689803727
1101	9	0.6463081140993125
1101	10-11	0.5934795224455485
1101	12-13	0.6107457313048386
1101	14-15	0.7405471185905128
1101	16-17	0.6945142271567732
1101	18-19	0.5780602303825688
1101	20-21	0.5947805158462103
1101	22-23	0.5548785818768565
1101	24-25	0.5545850847152352
1101	26-27	0.5572036087764403
1101	28-29	0.6198898185629034
1101	30-31	0.6284919388779002
1101	32-33	0.603368483944493
1101	34-35	0.6037205153411236
1101	36-37	0.5835213998932005
1101	38-39	0.6006253367800554
1101	40-41	0.6220197397562828
1101	42-43	0.5576768403882184
1101	44-45	0.5492879138344193
1101	46-47	0.47871704423935313
1101	48-49	0.5124016297853942
1101	50-51	0.5445648830616179
1101	52-53	0.480199831352877
1101	54-55	0.5221560959791987
1101	56-57	0.4603245671848484
1101	58-59	0.46131504147366087
1101	60-61	0.4386296855490457
1101	62-63	0.440318460928971
1101	64-65	0.3837906042797101
1101	66-67	0.4068894534696881
1101	68-69	0.37577552234994016
1101	70-71	0.4221186643548762
1101	72-73	0.39859685725712524
1101	74-75	0.3789599577369316
1101	76-77	0.3304682146952018
1101	78-79	0.3185546700827473
1101	80-81	0.315003366902765
1101	82-83	0.2900270804779339
1101	84-85	0.36429682819617426
1101	86-87	0.37527591722655274
1101	88-89	0.3110626755791017
1101	90-91	0.31832917797999016
1101	92-93	0.4148933190227808
1101	94-95	0.33377503593436586
1101	96-97	0.3263274199044517
1101	98-99	0.3005628620153473
1101	100	0.22366805433859938
1102	1	0.1405362956009668
1102	2	0.12068121702585799
1102	3	0.18329595258943954
1102	4	0.1727139903849988
1102	5	0.17297998782807156
1102	6	0.21509645926817456
1102	7	0.15589178184450247
1102	8	0.23422000473032512
1102	9	0.2740093044658565
1102	10-11	0.35180661334842966
1102	12-13	0.3082653555094481
1102	14-15	0.40540637888987874
1102	16-17	0.3961294439585856
1102	18-19	0.39826390215547036
1102	20-21	0.40031087982231384
1102	22-23	0.3693092561173117
1102	24-25	0.39521090795923186
1102	26-27	0.36063210401684387
1102	28-29	0.42761551060314673
1102	30-31	0.4349896165649483
1102	32-33	0.35849064771795724
1102	34-35	0.36773764332500036
1102	36-37	0.4380971711903072
1102	38-39	0.4154603466058475
1102	40-41	0.39525465631597
1102	42-43	0.35495070713751176
1102	44-45	0.2867436237098673
1102	46-47	0.2648094781560033
1102	48-49	0.2819365939442875
1102	50-51	0.3038213500156033
1102	52-53	0.3075284404062586
1102	54-55	0.3262642485826319
1102	56-57	0.3245154419216547
1102	58-59	0.3527247379272964
1102	60-61	0.352242445742462
1102	62-63	0.2943755361737104
1102	64-65	0.31100874968851855
1102	66-67	0.2988296330584532
1102	68-69	0.33072851250096136
1102	70-71	0.2965061386149088
1102	72-73	0.251938301992773
1102	74-75	0.24666963093844885
1102	76-77	0.3219453487207744
1102	78-79	0.31063342840697317
1102	80-81	0.29657303918965994
1102	82-83	0.3051777329219547
1102	84-85	0.2584507893967114
1102	86-87	0.26538510554743056
1102	88-89	0.24818779964941484
1102	90-91	0.2896620265185561
1102	92-93	0.23700118435633044
1102	94-95	0.19309352999606233
1102	96-97	0.21515561640993042
1102	98-99	0.22661994756573023
1102	100	0.10499441676112298
1103	1	0.17072349533494702
1103	2	0.13461488493756946
1103	3	0.22938082477161004
1103	4	0.2109002576671415
1103	5	0.2515614626643128
1103	6	0.23946344864092595
1103	7	0.2393293227367579
1103	8	0.34657673713286385
1103	9	0.30706863412805774
1103	10-11	0.35436971076228474
1103	12-13	0.30895324300712446
1103	14-15	0.31613590731957686
1103	16-17	0.32240713014595457
1103	18-19	0.31109051872822846
1103	20-21	0.34364069232050554
1103	22-23	0.34160193011855
1103	24-25	0.2809661834243471
1103	26-27	0.3226158123550249
1103	28-29	0.3689888393361187
1103	30-31	0.3717144587185075
1103	32-33	0.38532318112596897
1103	34-35	0.39516741262983146
1103	36-37	0.4064691711917874
1103	38-39	0.37003318598730317
1103	40-41	0.3716301806189506
1103	42-43	0.3593066573798396
1103	44-45	0.3221932277954238
1103	46-47	0.3356133922748654
1103	48-49	0.32572370028452724
1103	50-51	0.33461488485112767
1103	52-53	0.3852508450871355
1103	54-55	0.3703289735161519
1103	56-57	0.3432210856198239
1103	58-59	0.323194940260187
1103	60-61	0.32314841951273365
1103	62-63	0.30461081065833895
1103	64-65	0.3315253208259108
1103	66-67	0.33127383791114084
1103	68-69	0.2793469746759385
1103	70-71	0.21250385898557766
1103	72-73	0.2353388765920741
1103	74-75	0.2179955918113734
1103	76-77	0.32470095311904146
1103	78-79	0.32396592981302064
1103	80-81	0.30948527494597045
1103	82-83	0.31220628053164745
1103	84-85	0.2727418951159706
1103	86-87	0.28983408061176874
1103	88-89	0.258944607123496
1103	90-91	0.2549905901354208
1103	92-93	0.2521707347280966
1103	94-95	0.22230008924753974
1103	96-97	0.2580828479032391
1103	98-99	0.22545404232299404
1103	100	0.167125396037342
1104	1	0.0336817700523504
1104	2	0.017714124886296645
1104	3	0.07370224327475583
1104	4	0.09230027220250037
1104	5	0.1272287468523814
1104	6	0.058373444664532315
1104	7	0.05640532878976501
1104	8	0.10779240023466485
1104	9	0.1582539025855425
1104	10-11	0.2237698354701365
1104	12-13	0.24104715324617842
1104	14-15	0.27102875184814224
1104	16-17	0.23523211004410882
1104	18-19	0.18729174020779027
1104	20-21	0.17692478364516973
1104	22-23	0.1686610446002632
1104	24-25	0.20139312263367515
1104	26-27	0.2697418728230474
1104	28-29	0.27388779427140975
1104	30-31	0.2313469304844915
1104	32-33	0.23623359700857804
1104	34-35	0.2570173949698358
1104	36-37	0.1956461653716275
1104	38-39	0.21228476207875957
1104	40-41	0.22229499402703823
1104	42-43	0.18836337144382043
1104	44-45	0.13023118429951097
1104	46-47	0.1921329127097735
1104	48-49	0.22041115876731965
1104	50-51	0.21859593865557514
1104	52-53	0.1994454124726559
1104	54-55	0.2654364356651939
1104	56-57	0.24205410209153655
1104	58-59	0.24061912955502152
1104	60-61	0.2229747531900017
1104	62-63	0.22821221031180983
1104	64-65	0.15516292579644642
1104	66-67	0.15932600921176743
1104	68-69	0.20625592128348558
1104	70-71	0.17215816272555884
1104	72-73	0.1353105928155358
1104	74-75	0.0676683610152935
1104	76-77	0.08300228203992077
1104	78-79	0.10724085552759988
1104	80-81	0.11762136828520653
1104	82-83	0.10340098192950364
1104	84-85	0.1200105143816188
1104	86-87	0.17684968043382554
1104	88-89	0.10787935376612268
1104	90-91	0.18744392074685123
1104	92-93	0.1711550451015853
1104	94-95	0.1084960786913527
1104	96-97	0.06376693802432953
1104	98-99	0.04881876208447267
1104	100	0.07200418547319032
1105	1	0.0382323418249193
1105	2	0.1124123496719065
1105	3	0.1326746726526018
1105	4	0.17016575542170642
1105	5	0.14998943570105894
1105	6	0.12228664910945497
1105	7	0.025586126510887652
1105	8	0.16619259264711417
1105	9	0.1663329899785495
1105	10-11	0.20429045068885898
1105	12-13	0.2003779637760772
1105	14-15	0.2518076215999514
1105	16-17	0.17685270759406535
1105	18-19	0.1432799027811953
1105	20-21	0.17469723367257473
1105	22-23	0.16343563604780087
1105	24-25	0.16430204769433487
1105	26-27	0.19624827569947456
1105	28-29	0.18705457426586491
1105	30-31	0.2049860951448892
1105	32-33	0.17939151777905948
1105	34-35	0.20653539724128223
1105	36-37	0.2563405706361408
1105	38-39	0.18243993403820724
1105	40-41	0.219512082058543
1105	42-43	0.25702065631144677
1105	44-45	0.185181241949806
1105	46-47	0.20534741781795418
1105	48-49	0.23117892829920095
1105	50-51	0.20438637734646647
1105	52-53	0.20491754657903982
1105	54-55	0.1723218120164347
1105	56-57	0.11050520993193658
1105	58-59	0.17500425318672086
1105	60-61	0.19954946054293288
1105	62-63	0.13179149664045298
1105	64-65	0.1391362137727441
1105	66-67	0.08030746607728645
1105	68-69	0.08985702573268384
1105	70-71	0.0540859722000846
1105	72-73	0.13604909548032396
1105	74-75	0.15278181913016198
1105	76-77	0.1952777720458414
1105	78-79	0.2253411800022107
1105	80-81	0.22915965162399488
1105	82-83	0.18984804863320193
1105	84-85	0.1752171795679729
1105	86-87	0.14462029770454876
1105	88-89	0.1568510822252449
1105	90-91	0.17897841005249404
1105	92-93	0.16729780507547076
1105	94-95	0.12320781209253795
1105	96-97	0.15050298520555572
1105	98-99	0.1253625706932624
1105	100	0.1152686912509644
1106	1	0.17840184973178097
1106	2	0.15883448450259152
1106	3	0.13436433035960604
1106	4	0.12403844520083851
1106	5	0.1576495455658673
1106	6	0.16135943789442564
1106	7	0.04555653440438334
1106	8	0.10816052052147995
1106	9	0.1770871846302029
1106	10-11	0.1641750904079089
1106	12-13	0.18009636058806677
1106	14-15	0.2048260408283582
1106	16-17	0.15673252697135354
1106	18-19	0.2066474221650978
1106	20-21	0.13675975821591635
1106	22-23	0.2004370636730286
1106	24-25	0.16853631894353782
1106	26-27	0.2358590889402592
1106	28-29	0.1991837795267415
1106	30-31	0.1500787933220522
1106	32-33	0.18427982960766087
1106	34-35	0.1985046753798798
1106	36-37	0.19037067076686753
1106	38-39	0.18471606835152699
1106	40-41	0.16388340411138813
1106	42-43	0.17658021331919826
1106	44-45	0.14305066307427694
1106	46-47	0.04443333360776336
1106	48-49	0.05031042616658965
1106	50-51	0.060341027351888954
1106	52-53	0.06026409544580957
1106	54-55	0.14161007759086885
1106	56-57	0.09955784120096922
1106	58-59	0.16038172919567728
1106	60-61	0.09289944984234211
1106	62-63	0.13575383685749642
1106	64-65	0.1475897815363183
1106	66-67	0.11715203970039312
1106	68-69	0.1154617410584109
1106	70-71	0.10225687686899221
1106	72-73	0.1046749193953076
1106	74-75	0.09479923977218618
1106	76-77	0.1167005921609956
1106	78-79	0.1503194082606214
1106	80-81	0.14058318138930304
1106	82-83	0.08378037745116984
1106	84-85	0.16468522013356335
1106	86-87	0.06553920530220836
1106	88-89	0.04711820509847087
1106	90-91	0.07878080954309041
1106	92-93	0.09936497127898747
1106	94-95	0.04591211417649532
1106	96-97	0.06344307489558787
1106	98-99	0.08688384740565169
1106	100	0.0878916779867005
1107	1	0.14264102328854023
1107	2	0.1561932512183155
1107	3	0.23314637074269484
1107	4	0.2062023958031105
1107	5	0.23190926647912846
1107	6	0.18604441533531002
1107	7	0.10623335295522196
1107	8	0.16328477676122333
1107	9	0.19334904078707638
1107	10-11	0.3009393621787382
1107	12-13	0.22784764111494127
1107	14-15	0.2710404554679684
1107	16-17	0.23623378019307495
1107	18-19	0.196527792786938
1107	20-21	0.23298761805925494
1107	22-23	0.21283320090513058
1107	24-25	0.24163695520033457
1107	26-27	0.2630976604701729
1107	28-29	0.21715406045633756
1107	30-31	0.26425988010812773
1107	32-33	0.16288014908724335
1107	34-35	0.19711145613889158
1107	36-37	0.25149746122436056
1107	38-39	0.21899774879462797
1107	40-41	0.2389980059648309
1107	42-43	0.21981009956089537
1107	44-45	0.2135869849229266
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2216	46-47	-0.4287340620054536
2216	48-49	-0.48745678598142206
2216	50-51	-0.4578439366922069
2216	52-53	-0.41785385738834435
2216	54-55	-0.3657847029334249
2216	56-57	-0.37915289260522655
2216	58-59	-0.3792686631249964
2216	60-61	-0.34593196966503115
2216	62-63	-0.3734001073712605
2216	64-65	-0.36286800781724793
2216	66-67	-0.47392116346001245
2216	68-69	-0.43751451767120386
2216	70-71	-0.4987149836100926
2216	72-73	-0.47123059875737283
2216	74-75	-0.46239207730044996
2216	76-77	-0.4647424626437697
2216	78-79	-0.4410598432450499
2216	80-81	-0.3963800054448896
2216	82-83	-0.35110408315568264
2216	84-85	-0.4108051124517118
2216	86-87	-0.42210194277495106
2216	88-89	-0.3697403901914953
2216	90-91	-0.40507186407997153
2216	92-93	-0.4002373160297026
2216	94-95	-0.37289641313306987
2216	96-97	-0.32379339864120027
2216	98-99	-0.3908288166073248
2216	100	-0.41804077296639264
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	94.0
1	51.0
2	31.0
3	43.0
4	37.0
5	39.0
6	152.0
7	934.0
8	2464.0
9	2755.0
10	2301.0
11	2461.0
12	2845.0
13	3705.0
14	5054.0
15	6082.0
16	6499.0
17	7165.0
18	7665.0
19	8622.0
20	9716.0
21	11046.0
22	12576.0
23	14751.0
24	18860.0
25	24094.0
26	27665.0
27	26206.0
28	27077.0
29	34212.0
30	40604.0
31	43705.0
32	55735.0
33	75046.0
34	114132.0
35	180780.0
36	339550.0
37	753966.0
38	977039.0
39	342924.0
40	606.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.10414359354068	20.715112649145208	13.458098157391923	23.722645599922192
2	15.142214120658185	21.295883703741566	40.34865296167182	23.21324921392843
3	17.75322313640108	26.955495897170717	30.943112932151774	24.34816803427643
4	12.27603654974382	18.128555028286574	40.259594872198505	29.3358135497711
5	12.788984962417803	38.87628779661304	35.61476344180098	12.719963799168172
6	28.655483688074924	39.29776279801085	18.906272833151558	13.140480680762673
7	25.459528764504853	33.70826645378091	23.66029504034095	17.17190974137328
8	24.914035577324082	35.29338906466067	21.395342274176805	18.397233083838437
9	26.078946799040093	15.845081574219854	21.324848489789623	36.75112313695043
10-11	23.98409069208594	26.79437134857066	29.258203746445094	19.963334212898314
12-13	24.61470561720241	25.074899951788716	28.401353051617427	21.90904137939144
14-15	22.425749233997198	26.33110136009987	26.08622505728362	25.156924348619313
16-17	21.783264883876335	28.876916723294126	27.05413439005874	22.2856840027708
18-19	21.446436198000775	28.92734423359019	28.33476741261785	21.291452155791184
20-21	22.96052314913604	27.986594217156934	27.429331703600873	21.623550930106145
22-23	22.964985295699268	27.779871648182947	26.99704283483993	22.258100221277854
24-25	22.504504710720987	27.977087394288436	27.56244862481878	21.9559592701718
26-27	22.422864066383067	28.09871292342903	27.13738385730387	22.341039152884026
28-29	22.073114690716196	28.357957163581847	27.371655604718033	22.197272540983928
30-31	22.591224870856912	28.08477826053076	27.148560450661435	22.175436417950888
32-33	22.538913518111297	28.330640451243937	26.753277907783485	22.377168122861278
34-35	22.727005466278428	28.201593927465307	27.165191426364228	21.906209179892038
36-37	22.720725506731064	27.82342649392876	27.11510218981078	22.340745809529395
38-39	22.561847492897208	27.864131911630107	27.456945370236653	22.117075225236032
40-41	22.579429685893995	27.54062645581694	27.613727968604294	22.26621588968477
42-43	22.31221488638265	27.75366967520942	27.293755761860904	22.640359676547025
44-45	22.38046691829628	28.133087380522586	27.281912510489143	22.204533190691993
46-47	22.391676901903566	27.90862118499579	26.96732962564752	22.732372287453124
48-49	22.65675477653866	27.595104212537624	27.293469356006288	22.45467165491743
50-51	22.712482029862223	27.580521193980882	26.993071960061165	22.713924816095723
52-53	22.2700843216653	27.983175499748846	26.965713831378313	22.78102634720754
54-55	22.332753943580308	27.79380165360543	27.06956707619166	22.803877326622597
56-57	22.82614270140495	27.745060967548014	26.76838197411363	22.660414356933405
58-59	22.324600356980817	27.642598246964166	27.288267890248395	22.744533505806626
60-61	22.430267112575727	27.801485778022855	27.046536269005873	22.721710840395545
62-63	22.758850195231698	27.393082063807157	26.826441231479336	23.02162650948181
64-65	22.11512343413699	27.62577873631899	27.01249742015659	23.24660040938743
66-67	22.515205346874502	27.431792956663447	26.98916331344744	23.063838383014616
68-69	22.187125650362656	27.44071650540193	27.147870988626515	23.224286855608906
70-71	22.18412300295387	27.312837650693016	27.05336633604358	23.449673010309528
72-73	22.56341146233579	27.151579632802264	27.221541924366072	23.063466980495875
74-75	22.403533931564738	27.53878215744728	27.07313905868891	22.984544852299074
76-77	22.30148839593195	27.402265889977706	27.045983721149803	23.25026199294054
78-79	22.340364467178947	27.388988948297204	26.957541562294352	23.3131050222295
80-81	22.14815510722488	27.535865922593818	27.220255527887126	23.095723442294176
82-83	22.62848831519489	27.044934995194588	27.63163755398247	22.694939135628054
84-85	22.780701597556728	27.301508289523085	26.860629932867674	23.057160180052513
86-87	22.132878033969924	27.536829189343813	27.23209916969656	23.098193606989707
88-89	22.694196624950187	26.87159978402341	27.105642380591465	23.328561210434938
90-91	22.531602491972446	26.884744314060665	27.249663803276892	23.333989390689997
92-93	22.737639126964087	27.024310069835973	27.212442188894308	23.025608614305632
94-95	22.119617203118572	27.09417619453075	27.37384457608487	23.412362026265804
96-97	22.25256836586252	27.01554851713216	27.227924474329512	23.503958642675805
98-99	22.088236331915397	27.304020543782958	27.076157058004824	23.531586066296825
100	22.47331266677623	27.007054313066973	26.763452812127948	23.756180208028844
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1400.0
1	997.5
2	632.0
3	677.0
4	620.5
5	520.0
6	484.5
7	471.5
8	494.0
9	529.0
10	443.5
11	379.5
12	391.5
13	395.0
14	437.5
15	519.0
16	599.0
17	681.5
18	778.5
19	961.0
20	1343.5
21	1958.5
22	2952.5
23	4403.0
24	6450.5
25	9485.0
26	13763.0
27	18300.5
28	22729.5
29	28384.5
30	35562.5
31	43891.0
32	54323.0
33	64115.5
34	72741.5
35	79927.0
36	87214.5
37	98408.5
38	102047.0
39	99611.5
40	99417.0
41	99677.5
42	102176.5
43	106887.0
44	112025.0
45	120164.0
46	126782.0
47	132284.0
48	140142.5
49	136112.5
50	125195.0
51	122430.0
52	120958.0
53	117075.5
54	110013.5
55	97539.5
56	86479.0
57	72767.5
58	60037.5
59	54566.5
60	46074.5
61	35242.0
62	27078.0
63	20890.0
64	15119.0
65	10225.0
66	7605.0
67	5793.0
68	4228.5
69	3252.0
70	2587.0
71	1901.5
72	1478.5
73	1254.5
74	1008.5
75	798.5
76	679.0
77	510.5
78	359.5
79	285.5
80	205.5
81	143.0
82	104.0
83	76.0
84	59.0
85	53.5
86	41.5
87	29.5
88	23.5
89	22.5
90	17.0
91	13.0
92	13.0
93	12.5
94	10.0
95	6.5
96	8.0
97	7.0
98	3.0
99	5.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.061424348812540976
2	0.010347133796905831
3	0.012291140752688137
4	0.010033584287908685
5	0.012918239770682429
6	0.012385205605387282
7	0.003072785188172034
8	0.005612536211048921
9	0.007211638706934367
10-11	0.00666292706618936
12-13	0.0063180226062925
14-15	0.01040984369870526
16-17	0.009970874386109255
18-19	0.007870092675828375
20-21	0.0068197018206879335
22-23	0.0026965257773754587
24-25	0.003997756239713617
26-27	0.005095179521203629
28-29	0.006380732508091929
30-31	0.009014548383667957
32-33	0.01023739146875683
34-35	0.011711074161043418
36-37	0.017746902209238486
38-39	0.01813883909548492
40-41	0.017950709390086632
42-43	0.023751375306533838
44-45	0.02735719466000102
46-47	0.03000668801102691
48-49	0.0312608860470155
50-51	0.031778242736860786
52-53	0.03017914024097534
54-55	0.0365128403227177
56-57	0.03889581659109601
58-59	0.03645013042091827
60-61	0.037469166325158995
62-63	0.03217017962310722
64-65	0.03483535044958296
66-67	0.03710858438981228
68-69	0.039507238133640446
70-71	0.039758077740838164
72-73	0.035838708878373836
74-75	0.038409814852150434
76-77	0.03844116980305015
78-79	0.03709290691436242
80-81	0.04199995673016776
82-83	0.038158975244952716
84-85	0.03474128559688382
86-87	0.033142183100998375
88-89	0.030743529357170205
90-91	0.03296973087104994
92-93	0.03461586579328496
94-95	0.03848820222939972
96-97	0.03370657221719324
98-99	0.030618109553571346
100	0.027749131546247453
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3189289.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.470923005432226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.32575120246881	38.455634072458984
2	15.356408563617348	17.03668314144756
3	6.445347453243684	10.725881169664174
4	3.19411451729316	7.087219218372089
5	1.790924004769471	4.967210378857383
6	1.0580203012794993	3.5213617602275593
7	0.7124716268187652	2.766502112837314
8	0.5057387819023129	2.2443037625411426
9	0.3416475529837154	1.7056354595898604
>10	1.2505452861882718	10.57143622043268
>50	0.01620015806330106	0.5759670593849961
>100	0.0027362691310279397	0.24624885793883186
>500	0.0	0.0
>1k	9.428224069294904E-5	0.09591678624749549
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.270990179942928E-5
2	0.0	0.0	0.0	3.135495089971464E-5	6.270990179942928E-5
3	0.0	0.0	0.0	3.135495089971464E-5	6.270990179942928E-5
4	0.0	0.0	0.0	3.135495089971464E-5	6.270990179942928E-5
5	0.0	0.0	0.0	3.135495089971464E-5	6.270990179942928E-5
6	0.0	0.0	0.0	3.135495089971464E-5	1.2541980359885856E-4
7	0.0	0.0	0.0	3.135495089971464E-5	1.2541980359885856E-4
8	0.0	0.0	0.0	3.135495089971464E-5	1.2541980359885856E-4
9	0.0	0.0	0.0	6.270990179942928E-5	1.2541980359885856E-4
10-11	0.0	0.0	0.0	6.270990179942928E-5	1.567747544985732E-4
12-13	0.0	0.0	0.0	7.83873772492866E-5	6.89808919793722E-4
14-15	0.0	0.0	0.0	1.0974232814900124E-4	8.779386251920099E-4
16-17	0.0	0.0	0.0	1.2541980359885856E-4	0.0010033584287908685
18-19	0.0	0.0	0.0	1.2541980359885856E-4	0.0010033584287908685
20-21	0.0	0.0	0.0	2.978720335472891E-4	0.0011131007569398698
22-23	0.0	0.0	0.0	7.054863952435794E-4	0.0012855529868883002
24-25	0.0	0.0	0.0	0.0017401997749341624	0.0012855529868883002
26-27	0.0	0.0	0.0	0.0030727851881720346	0.0013482628886877296
28-29	0.0	0.0	0.0	0.009563260024412965	0.0013796178395874441
30-31	0.0	0.0	0.0	0.025836479541364865	0.0014109727904871587
32-33	0.0	0.0	0.0	0.0589159527405638	0.0015050376431863027
34-35	0.0	0.0	0.0	0.11044781454424482	0.0016931673485845904
36-37	0.0	0.0	0.0	0.18759667123299267	0.0016931673485845904
38-39	0.0	0.0	0.0	0.291945947827243	0.0017715547258338772
40-41	0.0	0.0	0.0	0.396875291013138	0.0017872322012837344
42-43	0.0	0.0	0.0	0.49942165793065474	0.0018029096767335916
44-45	0.0	0.0	0.0	0.6236342959198744	0.001959684431232165
46-47	0.0	0.0	0.0	0.7741380602385046	0.002100781710280881
48-49	0.0	0.0	0.0	0.9341580521551982	0.002210524038429882
50-51	0.0	0.0	0.0	1.089318026682436	0.0023829762683783124
52-53	0.0	0.0	0.0	1.2392574018848714	0.0026651708264757443
54-55	0.0	0.0	0.0	1.399057909145267	0.0027592356791748883
56-57	0.0	0.0	0.0	1.5810106892163112	0.0028219455809743174
58-59	0.0	0.0	0.0	1.7860407131495453	0.0028533005318740323
60-61	0.0	0.0	0.0	1.997921793854367	0.0028533005318740323
62-63	0.0	0.0	0.0	2.200647228896472	0.0029787203354728906
64-65	0.0	0.0	0.0	2.4110232719581073	0.0030414302372723198
66-67	0.0	0.0	0.0	2.643410490551342	0.0030414302372723198
68-69	0.0	0.0	0.0	2.8991728250403144	0.003057107712722177
70-71	0.0	0.0	0.0	3.1653450032279924	0.0031354950899714637
72-73	0.0	0.0	0.0	3.43256757227081	0.0032609148935703225
74-75	0.0	0.0	0.0	3.697203357864402	0.003433367123518753
76-77	0.0	0.0	0.0	3.983489737054246	0.0035274319762178966
78-79	0.0	0.0	0.0	4.293433426697925	0.003621496828917041
80-81	0.0	0.0	0.0	4.627818300567933	0.0037155616816161845
82-83	0.0	1.567747544985732E-5	0.0	4.9664047378585	0.0039193688624643295
84-85	0.0	3.135495089971464E-5	0.0	5.305226337280818	0.003966401288813901
86-87	0.0	3.135495089971464E-5	0.0	5.658925860905049	0.004185885945111904
88	0.0	3.135495089971464E-5	0.0	5.936150659284875	0.00438969312596005
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7230	0.0	26.012484	1
CTAACGC	870	0.0	25.925674	3
GTATCAA	9980	0.0	24.639465	1
GTACATG	11305	0.0	24.538137	1
TAACGCC	920	0.0	24.51667	4
TACATGG	11025	0.0	24.209053	2
TCTAACG	940	0.0	23.995037	2
CGCCTAC	940	0.0	23.491825	7
GTACTAG	1520	0.0	23.199455	1
ACATGGG	11315	0.0	22.633411	3
CAACGCA	10385	0.0	22.443123	5
ATCAACG	10475	0.0	22.295153	3
GCTATTG	2370	0.0	22.007074	8
GCATCGT	1430	0.0	21.686804	8
CTGTGCG	2015	0.0	21.220419	9
TATCAAC	11460	0.0	21.198935	2
GGGCTAT	2610	0.0	21.06362	6
AACGCAG	11380	0.0	21.016636	6
CATGGGG	7945	0.0	20.877998	4
ACGCCTA	1045	0.0	20.683746	6
>>END_MODULE