FastQCFastQC Report
Mon 16 Jan 2017
ERR1146500_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146500_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3189289
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122450.38394137376700577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108870.3413613504451933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59090.1852764048664138No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT42380.13288228191299065No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103250.043.7078551
GTATCAA165450.034.642681
ATCAACG197550.028.6553423
CAACGCA197600.028.6005065
TATCAAC203100.028.2648582
AACGCAG211200.027.461286
TCAACGC214550.026.494394
GTACATG109150.025.307271
CGCAGAG223800.025.1575898
TACATGG107200.025.0632972
ACGCAGA225800.024.9555747
GAGTACT148300.023.65706812-13
ACATGGG108200.023.5954763
GCAGAGT237950.023.36539
CTAACGC9150.023.123183
GTACTAG14550.022.962881
CATGGGG77500.022.7502274
CTCGTCG10500.021.484459
GTACTTT160400.021.41831414-15
TAACGCC9900.021.3714244
Produced by FastQC (version 0.11.5)