##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146500_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3189289
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57294462809736	34.0	31.0	34.0	31.0	34.0
2	32.60720586939597	34.0	31.0	34.0	31.0	34.0
3	32.73491489796002	34.0	31.0	34.0	31.0	34.0
4	36.19980879750941	37.0	37.0	37.0	35.0	37.0
5	36.23032688476962	37.0	37.0	37.0	35.0	37.0
6	36.31068711552951	37.0	37.0	37.0	35.0	37.0
7	36.29527208101869	37.0	37.0	37.0	35.0	37.0
8	36.30461366154024	37.0	37.0	37.0	35.0	37.0
9	38.11825457021926	39.0	39.0	39.0	37.0	39.0
10-11	38.09354075469486	39.0	38.5	39.0	37.0	39.0
12-13	38.0745804158858	39.0	38.0	39.0	36.0	39.0
14-15	39.517233935212516	41.0	39.0	41.0	37.0	41.0
16-17	39.50828209673065	41.0	39.0	41.0	37.0	41.0
18-19	39.57424115531707	41.0	39.5	41.0	37.0	41.0
20-21	39.615635177621094	41.0	40.0	41.0	37.0	41.0
22-23	39.572960305572806	41.0	40.0	41.0	37.0	41.0
24-25	39.55191157025908	41.0	39.5	41.0	37.0	41.0
26-27	39.46889290998715	41.0	39.0	41.0	36.0	41.0
28-29	39.38168021148287	41.0	39.0	41.0	36.0	41.0
30-31	39.2662582475279	41.0	39.0	41.0	35.5	41.0
32-33	39.125792143640794	41.0	39.0	41.0	35.0	41.0
34-35	39.03470318933154	41.0	39.0	41.0	35.0	41.0
36-37	38.9437906066211	41.0	39.0	41.0	35.0	41.0
38-39	38.82698980870031	41.0	38.5	41.0	35.0	41.0
40-41	38.72624964372937	40.5	38.0	41.0	35.0	41.0
42-43	38.62348238118277	40.0	38.0	41.0	35.0	41.0
44-45	38.451399826105444	40.0	38.0	41.0	35.0	41.0
46-47	38.186972864484844	40.0	38.0	41.0	34.0	41.0
48-49	37.93486432242422	40.0	38.0	41.0	33.5	41.0
50-51	37.762702125771604	40.0	37.5	41.0	33.0	41.0
52-53	37.62332811482434	40.0	37.0	41.0	33.0	41.0
54-55	37.4889072454707	40.0	37.0	41.0	33.0	41.0
56-57	37.28425755709188	40.0	36.0	41.0	33.0	41.0
58-59	37.06674011041332	39.5	35.5	41.0	32.0	41.0
60-61	36.88930949813579	39.0	35.0	41.0	32.0	41.0
62-63	36.638074505007225	39.0	35.0	41.0	32.0	41.0
64-65	36.34571279053105	38.5	35.0	41.0	31.0	41.0
66-67	36.09700328192271	37.0	35.0	40.5	31.0	41.0
68-69	35.788177396278606	37.0	35.0	40.0	31.0	41.0
70-71	35.51015618214593	36.5	35.0	39.5	31.0	41.0
72-73	35.19125548045348	36.0	35.0	39.0	31.0	41.0
74-75	34.87034727802968	35.5	35.0	39.0	30.5	40.5
76-77	34.564615655715116	35.0	35.0	37.5	30.0	39.5
78-79	34.27962690116826	35.0	35.0	37.0	30.0	39.0
80-81	34.02803681322075	35.0	35.0	36.5	30.0	39.0
82-83	33.7772500391153	35.0	34.0	36.0	30.0	38.0
84-85	33.57318245539993	35.0	34.0	36.0	30.0	37.0
86-87	33.385990576583055	35.0	34.0	35.5	29.5	37.0
88-89	33.23481612986468	35.0	34.0	35.0	29.0	36.0
90-91	33.152406539513976	35.0	34.0	35.0	29.0	36.0
92-93	33.07041600808205	35.0	34.0	35.0	29.0	36.0
94-95	32.99919950810353	35.0	34.0	35.0	29.0	36.0
96-97	32.95405559044664	35.0	34.0	35.0	29.0	35.5
98-99	32.90656789021001	35.0	34.0	35.0	29.0	35.0
100	32.35950896892693	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3179848123170359
1101	2	0.320650710560372
1101	3	0.32603084058100507
1101	4	0.1771441344955491
1101	5	0.15875173600682047
1101	6	0.15916355565106244
1101	7	0.15377081382580826
1101	8	0.16130512345350212
1101	9	0.2293166922666714
1101	10-11	0.24271585499152337
1101	12-13	0.26572417044006613
1101	14-15	0.3522758500215133
1101	16-17	0.36001503182328776
1101	18-19	0.35524808772122896
1101	20-21	0.3246955395527138
1101	22-23	0.34738053421806825
1101	24-25	0.28781317724389766
1101	26-27	0.3480929854396493
1101	28-29	0.32088950012256134
1101	30-31	0.33220219049226074
1101	32-33	0.41563539045135656
1101	34-35	0.43920335024401425
1101	36-37	0.4521565350817056
1101	38-39	0.4878850050409085
1101	40-41	0.4457446219139882
1101	42-43	0.3951477517766264
1101	44-45	0.4755392224914061
1101	46-47	0.49696122941382015
1101	48-49	0.5225729195450199
1101	50-51	0.5021338735618883
1101	52-53	0.5436160364864406
1101	54-55	0.4629478121269983
1101	56-57	0.48378880982181727
1101	58-59	0.4585679098457547
1101	60-61	0.44157531100824343
1101	62-63	0.4229709935157757
1101	64-65	0.40153651219181086
1101	66-67	0.4553144435342418
1101	68-69	0.4054588455377157
1101	70-71	0.3427949382346256
1101	72-73	0.31386888544234637
1101	74-75	0.3465843243580622
1101	76-77	0.34490883353489465
1101	78-79	0.2958070112240563
1101	80-81	0.3556194075393435
1101	82-83	0.325458166232508
1101	84-85	0.3428300577341048
1101	86-87	0.369478610512445
1101	88-89	0.3570764979690608
1101	90-91	0.35767942403754205
1101	92-93	0.2962469572146418
1101	94-95	0.3582225724957979
1101	96-97	0.36226646032481113
1101	98-99	0.33187625559296663
1101	100	0.3733070843930122
1102	1	0.2500471588997826
1102	2	0.2550718263140368
1102	3	0.2455776240278169
1102	4	0.18926177086984097
1102	5	0.166918638522489
1102	6	0.1794278053001861
1102	7	0.15949817666893296
1102	8	0.1708695359962462
1102	9	0.20127271034535
1102	10-11	0.230391590412097
1102	12-13	0.23766669456332323
1102	14-15	0.30205263616711875
1102	16-17	0.29917639054544054
1102	18-19	0.25999760577123965
1102	20-21	0.2931382018360438
1102	22-23	0.33034420617131843
1102	24-25	0.3052660466256256
1102	26-27	0.3049464655597376
1102	28-29	0.3714352484960415
1102	30-31	0.31576806643675326
1102	32-33	0.28595319725527446
1102	34-35	0.3075673997703703
1102	36-37	0.35347520691579604
1102	38-39	0.3356264582787176
1102	40-41	0.3745435568254578
1102	42-43	0.4062210397350583
1102	44-45	0.4225962842099946
1102	46-47	0.392250357536831
1102	48-49	0.36254961590267953
1102	50-51	0.3073344246563181
1102	52-53	0.36462296896577584
1102	54-55	0.3463586525073481
1102	56-57	0.3481432544859757
1102	58-59	0.43963254106142813
1102	60-61	0.4138091628658671
1102	62-63	0.4013891348023222
1102	64-65	0.3933194018563455
1102	66-67	0.3176756796192066
1102	68-69	0.3285210137039556
1102	70-71	0.34106091665589133
1102	72-73	0.27938725622274774
1102	74-75	0.28289733130728933
1102	76-77	0.18337082534576865
1102	78-79	0.2377306051635557
1102	80-81	0.2759344447008658
1102	82-83	0.23535029832254395
1102	84-85	0.29404357977240636
1102	86-87	0.24679604438079394
1102	88-89	0.21939087556923198
1102	90-91	0.2388793168588279
1102	92-93	0.22885382837538515
1102	94-95	0.24435368479062447
1102	96-97	0.2375880769972838
1102	98-99	0.33136132754247427
1102	100	0.28114311125725777
1103	1	0.2546337770769682
1103	2	0.21800853707408407
1103	3	0.19986606683696806
1103	4	0.17517446876671272
1103	5	0.10538851625517509
1103	6	0.12368632472463759
1103	7	0.1502624219691313
1103	8	0.09735724668461643
1103	9	0.2099096507148559
1103	10-11	0.18713316643644617
1103	12-13	0.22365314553798754
1103	14-15	0.2560915626887521
1103	16-17	0.21148051468879459
1103	18-19	0.2348811080959976
1103	20-21	0.26759651751258673
1103	22-23	0.27702354734928036
1103	24-25	0.24289625964976835
1103	26-27	0.2677423552942173
1103	28-29	0.3061754117295834
1103	30-31	0.3259159390364985
1103	32-33	0.30185916421662284
1103	34-35	0.3034478741026376
1103	36-37	0.30922261744925805
1103	38-39	0.2644970773675652
1103	40-41	0.25461343871778297
1103	42-43	0.24888334819215174
1103	44-45	0.2569416100475621
1103	46-47	0.28026338091287784
1103	48-49	0.21930615919032448
1103	50-51	0.17941984724752302
1103	52-53	0.10340030696929148
1103	54-55	0.12716763123118113
1103	56-57	0.17619019746887687
1103	58-59	0.18860977806996004
1103	60-61	0.09205592933657414
1103	62-63	0.11099615562489618
1103	64-65	0.08696004195012108
1103	66-67	0.1121749380030721
1103	68-69	0.1921532132021042
1103	70-71	0.13236319731274904
1103	72-73	0.12385872798754605
1103	74-75	0.0917241231790129
1103	76-77	0.12152065247945387
1103	78-79	0.15177905304350503
1103	80-81	0.15590129512193585
1103	82-83	0.10370585166623414
1103	84-85	0.11759291584614573
1103	86-87	0.1251418745742825
1103	88-89	0.12578871830253746
1103	90-91	0.1126082600920455
1103	92-93	0.08655996962054502
1103	94-95	0.13405201087121554
1103	96-97	0.12163762297173264
1103	98-99	0.05337601155432736
1103	100	0.11166460463111605
1104	1	0.13159940696500882
1104	2	0.0860501378340004
1104	3	0.0919388093772966
1104	4	0.06392699220266707
1104	5	0.05537709396080004
1104	6	0.031035778421873772
1104	7	0.059727661855831116
1104	8	0.0691578932767456
1104	9	0.09400021157149752
1104	10-11	0.08177233766161152
1104	12-13	0.1418609829215498
1104	14-15	0.16917374673327856
1104	16-17	0.19186553049021882
1104	18-19	0.13574453286786792
1104	20-21	0.13298032280540895
1104	22-23	0.20682064481351148
1104	24-25	0.15623052690322226
1104	26-27	0.08421918273401019
1104	28-29	0.17179796264848335
1104	30-31	0.21056572362152082
1104	32-33	0.18345779590213596
1104	34-35	0.16367752818417358
1104	36-37	0.1707220036311341
1104	38-39	0.19499964906184175
1104	40-41	0.2038911608692544
1104	42-43	0.22412474397570037
1104	44-45	0.26061624431426367
1104	46-47	0.2715748859754896
1104	48-49	0.28434096536312836
1104	50-51	0.26272560852781623
1104	52-53	0.2605830553469133
1104	54-55	0.21932679899379082
1104	56-57	0.24206124983366806
1104	58-59	0.30315539873683406
1104	60-61	0.1988393082433575
1104	62-63	0.21271252605256308
1104	64-65	0.19542167067902483
1104	66-67	0.2522348049443366
1104	68-69	0.2114379114012408
1104	70-71	0.18531123131253224
1104	72-73	0.10199345342891775
1104	74-75	0.08306970996090257
1104	76-77	0.16708840300265138
1104	78-79	0.188176419735953
1104	80-81	0.19496348772104
1104	82-83	0.16532189685361232
1104	84-85	0.19084896757907188
1104	86-87	0.1600619520746136
1104	88-89	0.1935384314143107
1104	90-91	0.15638585239737068
1104	92-93	0.16410022076751574
1104	94-95	0.1587755496854868
1104	96-97	0.09489458316474497
1104	98-99	0.06064464702181027
1104	100	0.10635734606972136
1105	1	0.15435869181622053
1105	2	0.10367138597478487
1105	3	0.18699300151931197
1105	4	0.11572359055441694
1105	5	0.015894255345777708
1105	6	0.05399858890510956
1105	7	0.06774508136033575
1105	8	0.09821367474388865
1105	9	0.09822807992751592
1105	10-11	0.10683018235703656
1105	12-13	0.1062354383325328
1105	14-15	0.1810428712739025
1105	16-17	0.1634355538916168
1105	18-19	0.14510835893806728
1105	20-21	0.12801470778323676
1105	22-23	0.15651573976477806
1105	24-25	0.17116741289732573
1105	26-27	0.1856503313464941
1105	28-29	0.1791569613434163
1105	30-31	0.162930492746014
1105	32-33	0.13758501384012334
1105	34-35	0.15899368465256458
1105	36-37	0.08543021083868751
1105	38-39	0.17760206851666283
1105	40-41	0.16262454347929634
1105	42-43	0.16180745264535545
1105	44-45	0.18013981181564986
1105	46-47	0.2342544464508407
1105	48-49	0.15177136704896554
1105	50-51	0.16554238923508535
1105	52-53	0.10855966820519569
1105	54-55	0.12118899642693037
1105	56-57	0.18127328973830004
1105	58-59	0.2224410156684371
1105	60-61	0.10359986943626609
1105	62-63	0.11799537430116658
1105	64-65	0.14632976543718712
1105	66-67	0.16529515113122528
1105	68-69	0.11560871266043904
1105	70-71	0.1713810654982737
1105	72-73	0.18435186352490973
1105	74-75	0.13760423836247782
1105	76-77	0.15818940809203497
1105	78-79	0.10289669592169304
1105	80-81	0.10124096017136708
1105	82-83	0.09774641903008074
1105	84-85	0.13001853656049178
1105	86-87	0.11341113941928427
1105	88-89	0.12042418360486806
1105	90-91	0.08748543296933065
1105	92-93	0.08558511872282537
1105	94-95	0.1328659645300192
1105	96-97	0.1457207492710353
1105	98-99	0.1615073116621204
1105	100	0.15145763252840538
1106	1	0.12392926921356917
1106	2	0.14371514671852026
1106	3	0.15536428032309146
1106	4	0.09128370655125195
1106	5	0.07746224307847172
1106	6	0.10547507745467044
1106	7	0.09026799625794979
1106	8	0.07553299213151377
1106	9	0.12372406344982068
1106	10-11	0.12900692707100347
1106	12-13	0.1419801049918945
1106	14-15	0.21153094915145232
1106	16-17	0.1985065859117654
1106	18-19	0.18141419743997034
1106	20-21	0.18638609724776245
1106	22-23	0.18564690361944258
1106	24-25	0.1925751669064013
1106	26-27	0.17971599621863277
1106	28-29	0.17261465198142645
1106	30-31	0.18194850750430192
1106	32-33	0.17419481407817727
1106	34-35	0.16485654714774256
1106	36-37	0.16883735184810433
1106	38-39	0.2592209216164463
1106	40-41	0.28668844488605316
1106	42-43	0.280229584633382
1106	44-45	0.2363281641318764
1106	46-47	0.2899567036459203
1106	48-49	0.151673929748938
1106	50-51	0.14985041536807842
1106	52-53	0.1966023018336358
1106	54-55	0.16731450460343922
1106	56-57	0.1824270042238112
1106	58-59	0.172290704864011
1106	60-61	0.18910852412233226
1106	62-63	0.16072897813405262
1106	64-65	0.17251052581666215
1106	66-67	0.15720331204698823
1106	68-69	0.1501444359817512
1106	70-71	0.08609888464084037
1106	72-73	0.002860107852477256
1106	74-75	0.034755576789045506
1106	76-77	0.009488869894987317
1106	78-79	0.05223973146186722
1106	80-81	0.0651648950159398
1106	82-83	0.08687732501473988
1106	84-85	0.05910057820872794
1106	86-87	0.11403099191763033
1106	88-89	0.10803380881619518
1106	90-91	0.07022481755747378
1106	92-93	0.043718475893903985
1106	94-95	0.019663720533010576
1106	96-97	0.02394250001754017
1106	98-99	0.05786285714653161
1106	100	-0.0018270019520301162
1107	1	0.04993903413409129
1107	2	0.09819155788784428
1107	3	0.08174233629431882
1107	4	0.07533365451196516
1107	5	0.05596117695798597
1107	6	0.09877832633534211
1107	7	0.012339580403029515
1107	8	0.03560733343945088
1107	9	0.06769057401738365
1107	10-11	0.04420012458047751
1107	12-13	0.04948216554513607
1107	14-15	0.1468614568244817
1107	16-17	0.11430511785202668
1107	18-19	0.10154573223685759
1107	20-21	0.06121023240654466
1107	22-23	0.05561616515694112
1107	24-25	0.06321542189704843
1107	26-27	0.08779315380365915
1107	28-29	0.12034924915828782
1107	30-31	0.11403638841108688
1107	32-33	0.09553284220753966
1107	34-35	0.11664694365598649
1107	36-37	0.13919447312385813
1107	38-39	0.11751589638124216
1107	40-41	0.10127412167645389
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2216	30-31	-0.37349893463044026
2216	32-33	-0.4148240975800519
2216	34-35	-0.3938084295641815
2216	36-37	-0.37469727447805923
2216	38-39	-0.3422592220979581
2216	40-41	-0.3283454634141094
2216	42-43	-0.3260030448706672
2216	44-45	-0.3642761516685198
2216	46-47	-0.34755851686723105
2216	48-49	-0.37983778799858925
2216	50-51	-0.36715745015629864
2216	52-53	-0.38353793164766614
2216	54-55	-0.39563596798325307
2216	56-57	-0.36330210613378
2216	58-59	-0.34456959676150944
2216	60-61	-0.2979946504808453
2216	62-63	-0.38619422584539365
2216	64-65	-0.3086367869096023
2216	66-67	-0.34507444115955366
2216	68-69	-0.37567835902319047
2216	70-71	-0.31507903291994666
2216	72-73	-0.375136626903803
2216	74-75	-0.34173780808927035
2216	76-77	-0.29878972419371763
2216	78-79	-0.34055181985652183
2216	80-81	-0.3394138175607111
2216	82-83	-0.26919183754307596
2216	84-85	-0.3025920520597509
2216	86-87	-0.3528312890571357
2216	88-89	-0.31370429188217486
2216	90-91	-0.3287926744295788
2216	92-93	-0.2770143884019163
2216	94-95	-0.25612651758952865
2216	96-97	-0.36333574461365004
2216	98-99	-0.3551149850624782
2216	100	-0.37842590662110354
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	24.0
8	93.0
9	133.0
10	249.0
11	283.0
12	225.0
13	200.0
14	344.0
15	683.0
16	1364.0
17	2630.0
18	4664.0
19	7228.0
20	10542.0
21	15709.0
22	22613.0
23	26688.0
24	25434.0
25	20659.0
26	17474.0
27	18504.0
28	21956.0
29	25260.0
30	29903.0
31	37837.0
32	48914.0
33	66121.0
34	99366.0
35	161992.0
36	295938.0
37	734533.0
38	1065396.0
39	425221.0
40	1107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.54419742247029	20.258662318522465	13.768461321375936	23.428678937631307
2	15.299239340632164	21.307343667521746	40.14274837860111	23.250668613244976
3	17.47074574825666	27.197800130436967	31.60268148296794	23.728772638338434
4	12.070495963206847	18.429468135374375	39.66279004505393	29.837245856364852
5	12.247932852564656	38.64415328430292	35.612776180338756	13.495137682793674
6	28.263951362329507	39.43372697218962	18.389951124647357	13.912370540833512
7	24.490262403395366	34.39821050968116	23.64527756665438	17.466249520269102
8	25.199503713837156	34.48194879799228	21.229277121013492	19.08927036715707
9	26.333330093321738	14.64006554438936	21.400443797975036	37.626160564313864
10-11	24.049545212114676	26.323594381067377	29.094211907418867	20.53264849939908
12-13	25.32870178901943	24.746581448090783	28.683540438009853	21.241176324879934
14-15	22.696316121918727	25.4892274562179	27.292153093080163	24.522303328783206
16-17	20.96901535107041	28.22771783930525	28.193101973511965	22.610164836112375
18-19	20.66010637480642	27.155723422994903	31.11530500998812	21.068865192210552
20-21	22.022149764414575	26.285686245429623	30.610929269815312	21.08123472034049
22-23	21.964305675162173	26.171296951704846	30.273436795735204	21.590960577397773
24-25	21.63849058520567	26.332028862859403	30.774884308069915	21.254596243865013
26-27	21.609173706114433	26.489681555983168	30.344051605232387	21.55709313267001
28-29	21.226261351477408	26.72733329936545	30.618547246054174	21.427858103102963
30-31	21.7001112004554	26.34006895901919	30.441865427225	21.51795441330041
32-33	21.712648689631102	26.72842754948639	29.9243815641711	21.634542196711408
34-35	21.933409662752627	26.61197558733701	30.21208928190405	21.242525468006317
36-37	21.973194141409675	26.30351722050108	30.115978114710114	21.607310523379127
38-39	21.907344824796677	26.246979712573363	30.413142083476263	21.4325333791537
40-41	21.822771680543525	26.091528958918765	30.41569125202791	21.670008108509798
42-43	21.835512991881192	26.250360193160738	29.869662595817655	22.044464219140423
44-45	21.894695420350565	26.748074992024044	29.617412748518667	21.739816839106723
46-47	22.124150473069896	26.598756770739428	28.828417116742834	22.44867563944784
48-49	22.566539348710883	26.576655176538626	28.71721476411687	22.139590710633613
50-51	22.50567802616807	26.728934863903746	28.31749662971305	22.447890480215136
52-53	22.0164832606435	27.14068935956595	28.308210541366417	22.534616838424128
54-55	22.06891513232849	27.09137893759378	28.19477609331247	22.64492983676526
56-57	22.649124630575777	26.999547072692714	27.830415487511416	22.52091280922009
58-59	22.01180201746488	26.996139000981596	28.41919158108749	22.57286740046604
60-61	22.232555616507252	27.245256964184282	28.06108123014704	22.461106189161423
62-63	22.39498478013026	26.924307016190628	27.871472683876974	22.809235519802133
64-65	21.81715560579562	27.0750117782814	28.054227913076673	23.053604702846307
66-67	22.192999770941274	26.89674656651005	28.097305902518816	22.812947760029864
68-69	21.945827775446965	26.84913779858157	28.225816060770825	22.979218365200637
70-71	21.981753623751896	26.729113179457396	28.04801839688251	23.241114799908193
72-73	22.323026636781762	26.564395112889933	28.167217706178878	22.94536054414943
74-75	22.270245998869296	26.96395697802651	27.846759696359015	22.919037326745183
76-77	22.194701969934776	26.791357553768357	27.781098917224924	23.23284155907194
78-79	22.102478486810988	26.86087967931164	27.732506977705988	23.304134856171384
80-81	21.894733541582564	27.004119258611837	27.937713360742798	23.1634338390628
82-83	22.354463739101192	26.523928187609595	28.315694831047423	22.80591324224179
84-85	22.552244101222108	26.70466579719238	27.543772762753765	23.199317338831747
86-87	21.797921667782905	27.032883201517077	27.892103190429964	23.277091940270054
88-89	22.27674862715901	26.39557730967847	27.805189916659884	23.522484146502638
90-91	22.086913415497936	26.384391630861927	27.919279187304756	23.60941576633538
92-93	22.2501002574555	26.55317533155509	27.81361300277272	23.38311140821669
94-95	21.663436844926384	26.557192928767652	28.016907219274767	23.762463007031194
96-97	21.807060467271757	26.42362113819456	27.9020587411763	23.86725965335739
98-99	21.635840719410588	26.738140534964423	27.72479033260668	23.90122841301831
100	21.99524906357764	26.394380399753427	27.521849855107728	24.088520681561214
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	432.0
1	293.5
2	373.5
3	531.5
4	305.5
5	235.5
6	529.5
7	1328.0
8	2501.0
9	3021.0
10	2230.0
11	1444.5
12	1486.0
13	1697.5
14	2038.0
15	2500.5
16	2917.5
17	3453.0
18	4057.5
19	4625.0
20	5396.0
21	6247.5
22	7324.5
23	8752.5
24	10797.5
25	13685.5
26	17603.0
27	21693.5
28	25534.5
29	30796.5
30	37676.0
31	45270.0
32	54389.0
33	63398.5
34	71260.5
35	77454.5
36	83825.5
37	94895.0
38	99286.0
39	97326.0
40	97230.0
41	97482.5
42	99520.5
43	103976.0
44	109630.0
45	118040.0
46	124595.5
47	130151.5
48	138278.5
49	134124.5
50	122942.5
51	119339.5
52	117384.0
53	113462.5
54	107331.0
55	96049.0
56	85190.5
57	72059.5
58	59767.0
59	54500.0
60	45926.0
61	34611.0
62	26043.5
63	19932.5
64	14507.5
65	9730.5
66	7103.0
67	5271.0
68	3794.0
69	2933.5
70	2347.0
71	1740.0
72	1316.0
73	1148.5
74	960.0
75	705.5
76	508.5
77	358.0
78	264.0
79	193.0
80	142.5
81	112.5
82	73.5
83	44.5
84	26.5
85	21.0
86	10.5
87	5.0
88	2.5
89	2.0
90	2.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005926085720046067
2	0.08914212540788871
3	2.8219455809743176E-4
4	0.0
5	0.03762594107965757
6	0.004891372340355484
7	3.135495089971464E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.567747544985732E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.5464678804586225E-4
24-25	0.0
26-27	0.0
28-29	4.703242634957196E-5
30-31	1.0974232814900122E-4
32-33	0.001677489873134733
34-35	0.0013325854132378722
36-37	7.36841346143294E-4
38-39	7.211638706934368E-4
40-41	0.001473682692286588
42-43	8.779386251920098E-4
44-45	5.173566898452915E-4
46-47	4.389693125960049E-4
48-49	9.249710515415818E-4
50-51	6.427764934441501E-4
52-53	0.001677489873134733
54-55	0.0014893601677364453
56-57	0.0011131007569398696
58-59	0.0027749131546247457
60-61	0.0018969745294327356
62-63	0.0011601331832894416
64-65	0.004907049815805341
66-67	0.004718920110407053
68-69	0.003982078764263759
70-71	0.0016618123976848758
72-73	0.0051735668984529155
74-75	0.004013433715163474
76-77	0.0038409814852150433
78-79	7.681962970430085E-4
80-81	4.703242634957196E-5
82-83	2.0380718084814516E-4
84-85	1.7245222994843052E-4
86-87	3.135495089971464E-5
88-89	2.665170826475744E-4
90-91	0.0
92-93	0.0
94-95	1.567747544985732E-5
96-97	3.135495089971464E-4
98-99	3.605819353467184E-4
100	7.211638706934368E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3189289.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.703223671425455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.99152241429293	34.636776674755
2	16.631094732210208	17.197624216802605
3	6.797443330213516	10.54349198687606
4	3.419763999910571	7.072512919634594
5	1.874324534334731	4.845431031577449
6	1.1964279664904518	3.711550965492273
7	0.7721448921324784	2.794566604527195
8	0.5300369090914987	2.1923693491895007
9	0.3840744662214418	1.7872099230177494
>10	1.367604124647745	10.865014823818193
>50	0.023094807862557156	0.7799845097253686
>100	0.010274111933436393	1.0289494907412533
>500	0.0011965694500694283	0.4179081934180819
>1k	6.979988458738284E-4	0.6293885054586623
>5k	9.971412083911835E-5	0.30464094681808945
>10k+	1.994282416782367E-4	1.1925798581479174
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12245	0.38394137376700577	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10887	0.3413613504451933	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5909	0.1852764048664138	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT	4238	0.13288228191299065	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2541980359885856E-4
2	0.0	0.0	0.0	3.135495089971464E-5	1.2541980359885856E-4
3	0.0	0.0	0.0	3.135495089971464E-5	1.2541980359885856E-4
4	0.0	0.0	0.0	3.135495089971464E-5	1.2541980359885856E-4
5	0.0	0.0	0.0	3.135495089971464E-5	1.2541980359885856E-4
6	0.0	0.0	0.0	3.135495089971464E-5	1.8812970539828784E-4
7	0.0	0.0	0.0	3.135495089971464E-5	1.8812970539828784E-4
8	0.0	0.0	0.0	3.135495089971464E-5	1.8812970539828784E-4
9	0.0	0.0	0.0	6.270990179942928E-5	1.8812970539828784E-4
10-11	0.0	0.0	0.0	9.406485269914392E-5	2.1948465629800248E-4
12-13	0.0	0.0	0.0	9.406485269914392E-5	4.8600173894557694E-4
14-15	0.0	0.0	0.0	9.406485269914392E-5	6.584539688940074E-4
16-17	0.0	0.0	0.0	9.406485269914392E-5	8.152287233925806E-4
18-19	0.0	0.0	0.0	9.406485269914392E-5	8.779386251920099E-4
20-21	0.0	0.0	0.0	2.8219455809743176E-4	0.0010503908551404405
22-23	0.0	0.0	0.0	7.368413461432941E-4	0.0011601331832894416
24-25	0.0	0.0	0.0	0.0018029096767335916	0.0011601331832894416
26-27	0.0	0.0	0.0	0.003088462663621892	0.001222843085088871
28-29	0.0	0.0	0.0	0.009563260024412965	0.001222843085088871
30-31	0.0	0.0	0.0	0.025820802065915006	0.001222843085088871
32-33	0.0	0.0	0.0	0.058947307691463516	0.0012541980359885856
34-35	0.0	0.0	0.0	0.1106045892987434	0.0013482628886877296
36-37	0.0	0.0	0.0	0.18809835044738812	0.0013482628886877296
38-39	0.0	0.0	0.0	0.29255736936978743	0.0013482628886877296
40-41	0.0	0.0	0.0	0.39775322963833004	0.0013482628886877296
42-43	0.0	0.0	0.0	0.5008326307211419	0.0014109727904871587
44-45	0.0	0.0	0.0	0.625750755105605	0.0015520700695358745
46-47	0.0	0.0	0.0	0.7767091662122811	0.0016774898731347332
48-49	0.0	0.0	0.0	0.9372935472451698	0.0017558772503840198
50-51	0.0	0.0	0.0	1.0930806207904018	0.0018499421030831636
52-53	0.0	0.0	0.0	1.2435687076335822	0.002069426759381166
54-55	0.0	0.0	0.0	1.40424715351917	0.002100781710280881
56-57	0.0	0.0	0.0	1.5875795514298014	0.002100781710280881
58-59	0.0	0.0	0.0	1.7936913211690757	0.0021321366611805953
60-61	0.0	0.0	0.0	2.0066384702044875	0.00216349161208031
62-63	0.0	0.0	0.0	2.2104613285280825	0.00216349161208031
64-65	0.0	0.0	0.0	2.4222013119538555	0.0022889114156791684
66-67	0.0	0.0	0.0	2.6559054384848784	0.0023359438420287406
68-69	0.0	0.0	0.0	2.91285926110804	0.0023829762683783124
70-71	0.0	0.0	0.0	3.17986234549456	0.00239865374382817
72-73	0.0	0.0	0.0	3.448433177426066	0.0026338158755760295
74-75	0.0	0.0	0.0	3.714668065515543	0.0028689780073238892
76-77	0.0	0.0	0.0	4.002255675167726	0.002900332958223604
78-79	0.0	0.0	0.0	4.314002274488138	0.0029630428600230336
80-81	0.0	0.0	0.0	4.65114638403732	0.002994397810922748
82-83	0.0	0.0	0.0	4.992633154286112	0.0031354950899714637
84-85	0.0	0.0	0.0	5.332708951744417	0.003182527516321036
86-87	0.0	0.0	0.0	5.687819448159136	0.003292269844470037
88	0.0	0.0	0.0	5.965624313130607	0.003386334697169181
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10325	0.0	43.707855	1
GTATCAA	16545	0.0	34.64268	1
ATCAACG	19755	0.0	28.655342	3
CAACGCA	19760	0.0	28.600506	5
TATCAAC	20310	0.0	28.264858	2
AACGCAG	21120	0.0	27.46128	6
TCAACGC	21455	0.0	26.49439	4
GTACATG	10915	0.0	25.30727	1
CGCAGAG	22380	0.0	25.157589	8
TACATGG	10720	0.0	25.063297	2
ACGCAGA	22580	0.0	24.955574	7
GAGTACT	14830	0.0	23.657068	12-13
ACATGGG	10820	0.0	23.595476	3
GCAGAGT	23795	0.0	23.3653	9
CTAACGC	915	0.0	23.12318	3
GTACTAG	1455	0.0	22.96288	1
CATGGGG	7750	0.0	22.750227	4
CTCGTCG	1050	0.0	21.48445	9
GTACTTT	16040	0.0	21.418314	14-15
TAACGCC	990	0.0	21.371424	4
>>END_MODULE