FastQCFastQC Report
Mon 16 Jan 2017
ERR1146499_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146499_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4263565
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63400.037.0777051
GTATCAA96050.032.5993541
ATCAACG99800.029.7605083
CAACGCA100650.029.462145
TATCAAC115950.026.6691362
AACGCAG117050.026.216536
GTACATG157350.025.8452931
TCAACGC118050.025.3982264
TACATGG158650.024.7639542
ACGCAGA122050.024.2552437
CGCAGAG122350.024.082818
GTGATCG12700.024.0521138
TGATCGC12750.023.957799
ACATGGG159350.023.7114323
GTACTAG20450.022.9899941
CATGGGG117100.021.5910664
CTGTGCG26600.021.5536989
GCAGAGT135200.020.646839
GCATCGT20000.020.2074768
GTATAAG23900.019.8680671
Produced by FastQC (version 0.11.5)