##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146499_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4263565
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.420466675188486	31.0	31.0	34.0	28.0	34.0
2	31.466368168422434	33.0	31.0	34.0	28.0	34.0
3	31.652950992889753	33.0	31.0	34.0	28.0	34.0
4	35.12179291273852	37.0	35.0	37.0	32.0	37.0
5	35.188359272111484	37.0	35.0	37.0	32.0	37.0
6	35.14750027265915	37.0	35.0	37.0	32.0	37.0
7	35.120825881627226	37.0	35.0	37.0	32.0	37.0
8	35.108388637208535	37.0	35.0	37.0	32.0	37.0
9	36.68260716090877	39.0	37.0	39.0	32.0	39.0
10-11	36.650416963268995	39.0	37.0	39.0	32.0	39.0
12-13	36.59697647391326	39.0	37.0	39.0	32.0	39.0
14-15	37.98935456595595	40.0	38.0	41.0	32.0	41.0
16-17	38.01277909449017	40.0	38.0	41.0	32.0	41.0
18-19	38.049727985852215	40.0	38.0	41.0	32.0	41.0
20-21	38.15327560386672	40.0	38.5	41.0	32.5	41.0
22-23	38.182984779169544	40.0	38.0	41.0	33.0	41.0
24-25	38.246500874268364	40.0	38.0	41.0	33.0	41.0
26-27	38.28460912405464	40.0	38.0	41.0	33.0	41.0
28-29	38.25025934869059	40.0	38.0	41.0	33.0	41.0
30-31	38.23472293726025	40.0	38.0	41.0	33.0	41.0
32-33	38.24273114166196	40.0	38.0	41.0	33.0	41.0
34-35	38.179426723880134	40.0	38.0	41.0	33.0	41.0
36-37	38.08289166460462	40.0	38.0	41.0	32.0	41.0
38-39	38.044316082902455	40.0	38.0	41.0	32.0	41.0
40-41	37.97896830469337	40.0	38.0	41.0	32.0	41.0
42-43	37.84335444633775	40.0	38.0	41.0	32.0	41.0
44-45	37.68728681279633	40.0	37.0	41.0	31.5	41.0
46-47	37.57789220992292	40.0	37.0	41.0	31.0	41.0
48-49	37.45581315167002	40.0	37.0	41.0	31.0	41.0
50-51	37.32599174634373	40.0	36.0	41.0	31.0	41.0
52-53	37.20258058690321	40.0	36.0	41.0	31.0	41.0
54-55	37.02049951155899	39.5	35.5	41.0	30.0	41.0
56-57	36.88495554776344	39.0	35.0	41.0	30.0	41.0
58-59	36.66237491395112	39.0	35.0	41.0	30.0	41.0
60-61	36.48008954947327	39.0	35.0	41.0	30.0	41.0
62-63	36.24298011171402	38.0	35.0	40.0	30.0	41.0
64-65	35.99535248084643	37.5	35.0	40.0	30.0	41.0
66-67	35.733562171563	37.0	35.0	40.0	29.5	41.0
68-69	35.44936866214072	36.5	35.0	40.0	29.0	41.0
70-71	35.19401521965773	36.0	34.0	39.0	29.0	41.0
72-73	34.886547759914535	35.5	34.0	39.0	29.0	41.0
74-75	34.573558747198646	35.0	34.0	38.5	29.0	40.0
76-77	34.26752142866357	35.0	34.0	37.0	29.0	39.5
78-79	33.99277201121596	35.0	34.0	37.0	28.5	39.0
80-81	33.713934582913595	35.0	34.0	36.5	28.0	39.0
82-83	33.455322951567524	35.0	34.0	36.0	28.0	37.5
84-85	33.231136853783156	35.0	34.0	36.0	28.0	37.0
86-87	33.03192000121963	35.0	33.0	35.0	27.0	37.0
88-89	32.8801050294765	35.0	33.0	35.0	27.0	36.0
90-91	32.70665839502857	35.0	33.0	35.0	27.0	36.0
92-93	32.60881199184251	35.0	33.0	35.0	27.0	36.0
94-95	32.50699801691776	35.0	33.0	35.0	26.5	36.0
96-97	32.42579484539347	35.0	33.0	35.0	26.0	35.0
98-99	32.3462677360378	35.0	33.0	35.0	26.0	35.0
100	31.430918726464824	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.30569578099196804
1101	2	0.3187119456698291
1101	3	0.36644525878741874
1101	4	0.3533305543345051
1101	5	0.3110422178511243
1101	6	0.2895408568870579
1101	7	0.28618066825408306
1101	8	0.37653347700099005
1101	9	0.4659261615293673
1101	10-11	0.5039735504222946
1101	12-13	0.4323479071578049
1101	14-15	0.5284961253042937
1101	16-17	0.5120877378112638
1101	18-19	0.46240837721924777
1101	20-21	0.44717876183359806
1101	22-23	0.4250951506392724
1101	24-25	0.437509663329422
1101	26-27	0.4930967175083296
1101	28-29	0.5129542279230961
1101	30-31	0.47803868143273576
1101	32-33	0.4924179133216171
1101	34-35	0.5053659907324288
1101	36-37	0.5058634622297404
1101	38-39	0.5824721833117295
1101	40-41	0.5969515827898348
1101	42-43	0.4701496467511319
1101	44-45	0.44218980084016124
1101	46-47	0.42235344277739273
1101	48-49	0.44819598261052107
1101	50-51	0.4128119874783138
1101	52-53	0.42254607242683306
1101	54-55	0.509051379315828
1101	56-57	0.45255208195035124
1101	58-59	0.4437010903600225
1101	60-61	0.45936599251360377
1101	62-63	0.5100084625589929
1101	64-65	0.43262346151967535
1101	66-67	0.40397606410622444
1101	68-69	0.3979930767920976
1101	70-71	0.4006872200312088
1101	72-73	0.4387189911982077
1101	74-75	0.3851648425568044
1101	76-77	0.3239705134026849
1101	78-79	0.31234262545433467
1101	80-81	0.29151749180240927
1101	82-83	0.28517489690130304
1101	84-85	0.2963009544984345
1101	86-87	0.32593721337838133
1101	88-89	0.2417642086283749
1101	90-91	0.2702570033166012
1101	92-93	0.31069941363629283
1101	94-95	0.30230613252908256
1101	96-97	0.21662630286615325
1101	98-99	0.2264231118097939
1101	100	0.1705052673335281
1102	1	0.27437666721866094
1102	2	0.28984751008690424
1102	3	0.33955545051072633
1102	4	0.24915016488050412
1102	5	0.24699676195546516
1102	6	0.2522131096759068
1102	7	0.24522937815108747
1102	8	0.2981079940471574
1102	9	0.3457028080405493
1102	10-11	0.338616689402528
1102	12-13	0.3630667080567562
1102	14-15	0.41343736944413223
1102	16-17	0.4043184434943683
1102	18-19	0.3394182916929225
1102	20-21	0.3782942642366507
1102	22-23	0.3709339964565217
1102	24-25	0.3269964866492998
1102	26-27	0.3971473519710784
1102	28-29	0.41057174431721677
1102	30-31	0.43817597058477276
1102	32-33	0.42361821917489806
1102	34-35	0.4373320203294071
1102	36-37	0.46596725583653154
1102	38-39	0.4597369755157459
1102	40-41	0.5087328333026591
1102	42-43	0.4545721409675636
1102	44-45	0.4302071719934446
1102	46-47	0.4049736689351704
1102	48-49	0.4269790733530172
1102	50-51	0.37736128907801714
1102	52-53	0.35345816079773584
1102	54-55	0.373581061354308
1102	56-57	0.31539094002332746
1102	58-59	0.38988740701952906
1102	60-61	0.310561331678592
1102	62-63	0.29345389431709634
1102	64-65	0.28323966530387423
1102	66-67	0.2554291372108466
1102	68-69	0.27486604901334033
1102	70-71	0.3240888281912859
1102	72-73	0.3773789367370526
1102	74-75	0.3187754255288553
1102	76-77	0.32534081875533616
1102	78-79	0.3529473275433972
1102	80-81	0.35610464077169723
1102	82-83	0.4101540985045986
1102	84-85	0.33585265012082743
1102	86-87	0.374567811626477
1102	88-89	0.3722016261748067
1102	90-91	0.41012951875207193
1102	92-93	0.4244692920739439
1102	94-95	0.30502534763030553
1102	96-97	0.3882371197357841
1102	98-99	0.3601092856588366
1102	100	0.3395925454034696
1103	1	0.17656219515269456
1103	2	0.1995735126272784
1103	3	0.2695013947613951
1103	4	0.2057801148570917
1103	5	0.21648427402448078
1103	6	0.1960527032161039
1103	7	0.16921194052353883
1103	8	0.20715409936737927
1103	9	0.34008889557367183
1103	10-11	0.3501178173374555
1103	12-13	0.33963937641252073
1103	14-15	0.3960361449570797
1103	16-17	0.3918960380247327
1103	18-19	0.30032756181709175
1103	20-21	0.3231229010147203
1103	22-23	0.3114911537468785
1103	24-25	0.3789800615869652
1103	26-27	0.29154318432433257
1103	28-29	0.3812045527743777
1103	30-31	0.3657495551776506
1103	32-33	0.3274217232778085
1103	34-35	0.30789056653665625
1103	36-37	0.38932872593785106
1103	38-39	0.35924043772713077
1103	40-41	0.34189927259018305
1103	42-43	0.30105706113844377
1103	44-45	0.23107737322958144
1103	46-47	0.19117791588458743
1103	48-49	0.17735318483824614
1103	50-51	0.23591410488300113
1103	52-53	0.19429332548519795
1103	54-55	0.18956189430120673
1103	56-57	0.22859315512175726
1103	58-59	0.2737134698209758
1103	60-61	0.21128177631121758
1103	62-63	0.17088981932454317
1103	64-65	0.14328164643701058
1103	66-67	0.16205079848985093
1103	68-69	0.21594124990224373
1103	70-71	0.19024036350450046
1103	72-73	0.23992768884002658
1103	74-75	0.1872405155508332
1103	76-77	0.16847553697322581
1103	78-79	0.21087457070017734
1103	80-81	0.2510750814839895
1103	82-83	0.1195635624050837
1103	84-85	0.09124254373427476
1103	86-87	0.17982990248404462
1103	88-89	0.09546385592405926
1103	90-91	0.21963284655214466
1103	92-93	0.19497205758668912
1103	94-95	0.10169463531755696
1103	96-97	0.10432906603398351
1103	98-99	0.08937466063279942
1103	100	0.03256238574523351
1104	1	0.19328422469789075
1104	2	0.15833380477057446
1104	3	0.22509668882548084
1104	4	0.23839103229116887
1104	5	0.16939228572780252
1104	6	0.1671378815122182
1104	7	0.1750550353123117
1104	8	0.1836014402095003
1104	9	0.24081430531479953
1104	10-11	0.3275645230088102
1104	12-13	0.317907826728522
1104	14-15	0.37371965176777877
1104	16-17	0.2781700369689091
1104	18-19	0.3009442221079297
1104	20-21	0.2758757772756226
1104	22-23	0.2606581002356947
1104	24-25	0.19345743344658928
1104	26-27	0.23037277833989123
1104	28-29	0.23553056304059794
1104	30-31	0.27570331238270285
1104	32-33	0.2604070781642065
1104	34-35	0.23614013059708583
1104	36-37	0.2713890316245724
1104	38-39	0.24351292348217868
1104	40-41	0.19283426208714616
1104	42-43	0.1733115140684376
1104	44-45	0.1665324311630485
1104	46-47	0.16209697925621214
1104	48-49	0.20011790378499228
1104	50-51	0.18016377434646103
1104	52-53	0.15895938824942135
1104	54-55	0.19080266855943506
1104	56-57	0.17790186064174662
1104	58-59	0.20565902136319636
1104	60-61	0.20955515223936771
1104	62-63	0.21919853526731004
1104	64-65	0.21338226828704165
1104	66-67	0.23672536432169977
1104	68-69	0.19191031831978478
1104	70-71	0.2078249164185948
1104	72-73	0.21050589341759718
1104	74-75	0.19179027374397606
1104	76-77	0.17192292423845856
1104	78-79	0.18737439655941301
1104	80-81	0.23024027713201178
1104	82-83	0.16607906603686473
1104	84-85	0.13822015984871427
1104	86-87	0.1504049060013415
1104	88-89	0.15948792208276785
1104	90-91	0.2184240365043877
1104	92-93	0.21145807465956779
1104	94-95	0.14482066458602105
1104	96-97	0.09837589807144553
1104	98-99	0.12325482165819324
1104	100	0.2164523185096705
1105	1	0.25854151632651323
1105	2	0.18802722562990937
1105	3	0.2503665122203351
1105	4	0.25092065845487355
1105	5	0.1847094864864829
1105	6	0.1831188964382946
1105	7	0.16598693505784468
1105	8	0.26490175231254653
1105	9	0.30959112985974
1105	10-11	0.3426052753247788
1105	12-13	0.32321031482646134
1105	14-15	0.3974002521211517
1105	16-17	0.32955398137837477
1105	18-19	0.33735438976761145
1105	20-21	0.3487477698058967
1105	22-23	0.36096019847585836
1105	24-25	0.36658858213654355
1105	26-27	0.3354583427583506
1105	28-29	0.35559103055848595
1105	30-31	0.37735476342658103
1105	32-33	0.3646635366038282
1105	34-35	0.3387290142975772
1105	36-37	0.34452640621222486
1105	38-39	0.36023375020254633
1105	40-41	0.36197989293039257
1105	42-43	0.36649733239144666
1105	44-45	0.25353008731390503
1105	46-47	0.2712538752854954
1105	48-49	0.3147856625978349
1105	50-51	0.3572476359495127
1105	52-53	0.3645237610890675
1105	54-55	0.36749277240352285
1105	56-57	0.33910899321713117
1105	58-59	0.3449757033252183
1105	60-61	0.28935406841504374
1105	62-63	0.305586033732304
1105	64-65	0.2954542847747703
1105	66-67	0.3696410997670583
1105	68-69	0.342570678817566
1105	70-71	0.32875899480594484
1105	72-73	0.3206060509629296
1105	74-75	0.34724420940818845
1105	76-77	0.3233367140978203
1105	78-79	0.2521585227786005
1105	80-81	0.38191377506238666
1105	82-83	0.2908521523399443
1105	84-85	0.3006682622325485
1105	86-87	0.2684553040096702
1105	88-89	0.2643382483764043
1105	90-91	0.2568012893550389
1105	92-93	0.23659971946402436
1105	94-95	0.2252224171748125
1105	96-97	0.24255536938600386
1105	98-99	0.2584991723740728
1105	100	0.23552338922975835
1106	1	0.1784806943957875
1106	2	0.15809487684247614
1106	3	0.18961180907584207
1106	4	0.22569183068677034
1106	5	0.1813718218346949
1106	6	0.23347525876501152
1106	7	0.14179387759420337
1106	8	0.17752410480201775
1106	9	0.22132366846719975
1106	10-11	0.2322882894667444
1106	12-13	0.2653473835707558
1106	14-15	0.2339482688129806
1106	16-17	0.24038278136820423
1106	18-19	0.24616830652901456
1106	20-21	0.24046803985869047
1106	22-23	0.25703318753112825
1106	24-25	0.23564817285775774
1106	26-27	0.254405263610451
1106	28-29	0.272746589626486
1106	30-31	0.29892966663323506
1106	32-33	0.2954238772809106
1106	34-35	0.273583925535398
1106	36-37	0.2576093429507438
1106	38-39	0.2684615957907326
1106	40-41	0.3034750135633715
1106	42-43	0.24240437419733496
1106	44-45	0.21231741563471473
1106	46-47	0.20553783128727332
1106	48-49	0.2018698582857965
1106	50-51	0.19789823300575193
1106	52-53	0.20993678202855648
1106	54-55	0.27936384638456246
1106	56-57	0.21706068459875638
1106	58-59	0.23935106583729748
1106	60-61	0.19046373155447327
1106	62-63	0.15186990517246812
1106	64-65	0.19050522288160465
1106	66-67	0.16553043566232617
1106	68-69	0.20538390972070886
1106	70-71	0.1746929131315227
1106	72-73	0.21612767799421562
1106	74-75	0.17658608642256013
1106	76-77	0.18739809148447506
1106	78-79	0.1799412292099305
1106	80-81	0.23023691513326128
1106	82-83	0.1471301912738312
1106	84-85	0.23380602217497426
1106	86-87	0.16740887861252673
1106	88-89	0.10792688249512139
1106	90-91	0.1740599626648418
1106	92-93	0.19339815191943188
1106	94-95	0.14203626569542394
1106	96-97	0.15782602311660554
1106	98-99	0.1282383915137899
1106	100	0.009526408966817002
1107	1	0.11035903996573992
1107	2	0.13711397953735371
1107	3	0.1754269710749199
1107	4	0.18941933317776005
1107	5	0.164012899785817
1107	6	0.15225862111065425
1107	7	0.16967378279643697
1107	8	0.15318889858223628
1107	9	0.24980127193597923
1107	10-11	0.2896082896866474
1107	12-13	0.21306102498021318
1107	14-15	0.2652379506982001
1107	16-17	0.25858463716051716
1107	18-19	0.2454511671493833
1107	20-21	0.24921942227417304
1107	22-23	0.2648922364375679
1107	24-25	0.22798269064447396
1107	26-27	0.2548161633406991
1107	28-29	0.2711485525890609
1107	30-31	0.28059309599452575
1107	32-33	0.23269037586908325
1107	34-35	0.26604353753174337
1107	36-37	0.26102474679862553
1107	38-39	0.21914893453485718
1107	40-41	0.24522393797099795
1107	42-43	0.221994867514546
1107	44-45	0.20382436417203564
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2216	48-49	-0.24141548780367117
2216	50-51	-0.2577514449392524
2216	52-53	-0.20619193958005866
2216	54-55	-0.16437913529133397
2216	56-57	-0.18744828951777492
2216	58-59	-0.14030075525000996
2216	60-61	-0.1830166626623182
2216	62-63	-0.1886198425297252
2216	64-65	-0.20769405900230709
2216	66-67	-0.25368693219474636
2216	68-69	-0.2295005170120703
2216	70-71	-0.2107638061331798
2216	72-73	-0.1860866397371339
2216	74-75	-0.22068134878706758
2216	76-77	-0.16397026284838034
2216	78-79	-0.19858876894574706
2216	80-81	-0.18283654468748978
2216	82-83	-0.14825866765464468
2216	84-85	-0.12822434834583873
2216	86-87	-0.17471676602272623
2216	88-89	-0.17741267015311024
2216	90-91	-0.17705920468469571
2216	92-93	-0.11996902695357647
2216	94-95	-0.12422193742683163
2216	96-97	-0.10898991060940233
2216	98-99	-0.10977994309189398
2216	100	-0.08157202149661913
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	115.0
1	70.0
2	64.0
3	51.0
4	42.0
5	87.0
6	202.0
7	969.0
8	3030.0
9	4116.0
10	4295.0
11	5163.0
12	6113.0
13	7260.0
14	8986.0
15	9853.0
16	10647.0
17	11652.0
18	12987.0
19	14133.0
20	16204.0
21	18054.0
22	20443.0
23	23677.0
24	28472.0
25	35150.0
26	39585.0
27	38721.0
28	40597.0
29	51057.0
30	60468.0
31	64793.0
32	81740.0
33	109806.0
34	165357.0
35	253094.0
36	473023.0
37	1011625.0
38	1230867.0
39	400443.0
40	554.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.41398028504382	20.369821468006908	13.222627679839777	23.993570567109494
2	14.976966578492492	21.14254402785919	40.166116487022634	23.714372906625684
3	17.695754835374196	26.889545589531654	30.78646287574518	24.628236699348964
4	12.03088524504027	18.064448330993898	40.067523062834496	29.837143361131336
5	12.861452657856212	38.81302103357673	35.457130250447975	12.868396058119075
6	28.8228771528494	39.034434096260085	18.905290694633116	13.237398056257394
7	25.670727874377143	33.52733997149711	23.608584453636837	17.193347700488903
8	25.486452786002996	35.02797824789741	21.286079930345235	18.19948903575436
9	26.34588270009474	15.575865602781732	21.123309180358707	36.954942516764824
10-11	24.077800142004104	26.73930987505562	29.150881591234118	20.032008391706153
12-13	24.78730081734845	24.88857218050439	28.303322920424517	22.02080408172265
14-15	22.41576552274167	26.03413009312472	25.970948370903802	25.57915601322981
16-17	21.957903052981013	28.76666153739334	26.75910227082938	22.51633313879626
18-19	21.68620183601154	28.597709917307583	28.170085981016413	21.546002265664463
20-21	23.138492103749307	27.74835908926449	27.253221275046137	21.85992753194007
22-23	23.199747059276767	27.535930705875604	26.957596190596373	22.306726044251256
24-25	22.616016416027183	27.848021101495533	27.358118044201635	22.177844438275653
26-27	22.450401298980786	28.06078798501411	26.930970242245373	22.557840473759732
28-29	22.19544450092147	28.26665199879811	27.137558414434682	22.400345085845732
30-31	22.645122881766426	27.836772837444872	27.08243855559827	22.43566572519043
32-33	22.6502768643836	28.1719303661328	26.567205351601114	22.61058741788249
34-35	22.845346077952104	28.133359668292112	27.01302923649575	22.00826501726003
36-37	22.881448774358702	27.750502727348646	26.910551597232583	22.45749690106007
38-39	22.645221496842293	27.84191743023923	27.08148055702109	22.431380515897377
40-41	22.721420741106705	27.490966476202004	27.326083993531846	22.461528789159445
42-43	22.530243762420117	27.536471928793745	27.086359488660445	22.8469248201257
44-45	22.50873933097782	27.957987865820183	27.138191697518266	22.39508110568373
46-47	22.658824937122265	27.784697717632046	26.748399902398738	22.80807744284695
48-49	22.769023436940945	27.596280608877617	26.943824590241245	22.690871363940197
50-51	22.70623407870742	27.64006191670807	26.83212982096593	22.821574183618583
52-53	22.564434158248066	27.784508070113862	26.866809541512048	22.784248230126025
54-55	22.42050259052222	27.7771462244589	26.887418601478164	22.914932583540722
56-57	22.916950188133114	27.571555343233822	26.718973053181276	22.792521415451787
58-59	22.47451453107265	27.551394742476372	27.177601999067335	22.796488727383643
60-61	22.51103337682277	27.7674949368887	26.98339459043967	22.738077095848865
62-63	22.721568696458057	27.426602262362486	26.795498737429284	23.056330303750173
64-65	22.24006838033582	27.535579172810042	26.93032967280488	23.294022774049257
66-67	22.634936172828674	27.465826900091926	26.749797863808777	23.14943906327062
68-69	22.42257364573905	27.256739848505557	27.069897093355095	23.250789412400298
70-71	22.40507184503744	27.113650052641074	26.989432600300194	23.49184550202129
72-73	22.595869299803955	27.2232830796993	27.048154587027938	23.132693033468804
74-75	22.52331986133027	27.615080040122713	26.95054464825167	22.911055450295347
76-77	22.47782135093633	27.43485309632935	26.837306283275016	23.250019269459308
78-79	22.345376695836045	27.42967327252935	26.85251914823426	23.372430883400344
80-81	22.44873824251705	27.47054381834334	26.997616474343662	23.08310146479595
82-83	22.74048062454583	27.01979134721896	27.439110089764856	22.800617938470353
84-85	22.86808659901937	27.411385228847806	26.763576042791538	22.95695212934129
86-87	22.223783784291083	27.654938370958636	27.064186926956907	23.057090917793378
88-89	22.72058765736989	27.113670919427623	26.873443516352953	23.292297906849534
90-91	22.590212967336125	27.146278990049293	27.047407750482215	23.216100292132367
92-93	22.740562814365113	27.064807238279588	27.098030589649962	23.096599357705337
94-95	22.14614531892995	27.12821265461504	27.428570557065246	23.29707146938976
96-97	22.45340444691375	26.990097843740013	27.18108341846347	23.375414290882762
98-99	22.190467721814937	27.356622017717086	26.961639411263583	23.491270849204394
100	22.516715083201206	27.0059624833248	26.61765264815999	23.859669785314
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1981.0
1	1450.5
2	993.0
3	1071.5
4	983.5
5	859.5
6	774.5
7	789.0
8	867.5
9	874.5
10	746.5
11	598.5
12	602.0
13	619.5
14	650.0
15	778.5
16	900.0
17	1053.5
18	1338.5
19	1657.5
20	2040.5
21	2786.0
22	4252.5
23	6329.0
24	8797.0
25	12259.0
26	17269.0
27	23363.0
28	30627.5
29	39749.0
30	49311.5
31	59397.5
32	71777.5
33	82451.5
34	92167.5
35	102350.5
36	112569.0
37	127981.5
38	134360.5
39	132241.5
40	130984.0
41	128226.5
42	128976.0
43	136068.5
44	146705.0
45	160984.5
46	172239.0
47	176660.5
48	175814.0
49	169223.0
50	164075.0
51	163655.5
52	161243.5
53	156006.0
54	149985.0
55	136559.0
56	123340.0
57	107996.0
58	91862.5
59	82004.0
60	67071.0
61	50627.0
62	40397.5
63	31769.0
64	22420.0
65	14654.5
66	10569.5
67	7825.0
68	5740.0
69	4272.5
70	3083.0
71	2438.0
72	1977.5
73	1588.0
74	1309.5
75	1047.5
76	794.0
77	610.5
78	467.0
79	333.5
80	250.5
81	199.5
82	159.5
83	135.0
84	109.0
85	85.5
86	66.0
87	46.5
88	36.0
89	31.5
90	26.0
91	20.5
92	16.5
93	15.5
94	16.5
95	10.0
96	10.5
97	15.0
98	11.0
99	8.0
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059480739709609216
2	0.009663274747775629
3	0.011797638830415392
4	0.010062002103873166
5	0.012290184387947644
6	0.012946911797990648
7	0.003213273399138983
8	0.005934001240745714
9	0.007552365215494544
10-11	0.007224001510473043
12-13	0.006262364945767216
14-15	0.010906365916785602
16-17	0.010624911312481456
18-19	0.00836154720286896
20-21	0.007212274235293704
22-23	0.002709000566427391
24-25	0.00399900083615472
26-27	0.005218637454806013
28-29	0.006520364999712682
30-31	0.009428729244188841
32-33	0.010953275017502958
34-35	0.012595093542610469
36-37	0.019162367643040506
38-39	0.019185822193399184
40-41	0.01951418589842069
42-43	0.025201914360400274
44-45	0.028731824189381423
46-47	0.031077279225249294
48-49	0.031945097588520405
50-51	0.03254318862266671
52-53	0.031112461050787313
54-55	0.03721064414404377
56-57	0.03961473555580834
58-59	0.036999553190815666
60-61	0.03777355335265206
62-63	0.03348137063701386
64-65	0.03567437109555032
66-67	0.03722237141922312
68-69	0.03969682648206372
70-71	0.03949746280401495
72-73	0.03638973488149002
74-75	0.038465462588233086
76-77	0.038453735313053745
78-79	0.0379611897555215
80-81	0.042476190699567144
82-83	0.03874691719253723
84-85	0.03535773466570816
86-87	0.03403255257044281
88-89	0.031745733910471634
90-91	0.034149825322236205
92-93	0.035240461913914764
94-95	0.03868828081664053
96-97	0.034314007174746954
98-99	0.03171055208493362
100	0.02924782429727235
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4263565.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.63929811375825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.51841380311039	36.594005085775336
2	14.625307210927529	15.397318125626247
3	6.126052095431974	9.67413247535571
4	3.2724585559639743	6.890396859692259
5	1.872195526378426	4.927552922013925
6	1.1955543556818593	3.7759885283963737
7	0.7821094849342954	2.8818786034538
8	0.5521515273728209	2.325189508267589
9	0.4108513607028955	1.946423453083464
>10	1.5996995477522566	13.280829003716594
>50	0.03374319393119039	1.197686982575311
>100	0.011379819027192985	0.9952160637675702
>500	0.0	0.0
>1k	8.351878518994966E-5	0.11338238827587047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.690910071735742E-5	0.0
3	0.0	0.0	0.0	7.036365107603613E-5	0.0
4	0.0	0.0	0.0	1.1727275179339355E-4	0.0
5	0.0	0.0	0.0	1.64181852510751E-4	0.0
6	0.0	0.0	0.0	1.64181852510751E-4	2.345455035867871E-5
7	0.0	0.0	0.0	2.110909532281084E-4	2.345455035867871E-5
8	0.0	0.0	0.0	2.110909532281084E-4	2.345455035867871E-5
9	0.0	0.0	0.0	2.345455035867871E-4	2.345455035867871E-5
10-11	0.0	0.0	0.0	2.580000539454658E-4	3.5181825538018066E-5
12-13	0.0	0.0	0.0	2.8145460430414453E-4	4.690910071735742E-5
14-15	0.0	0.0	0.0	3.987273560975381E-4	5.863637589669678E-5
16-17	0.0	0.0	0.0	4.690910071735742E-4	7.036365107603613E-5
18-19	0.0	0.0	0.0	4.8081828235291355E-4	7.036365107603613E-5
20-21	0.0	0.0	0.0	6.684546852223432E-4	7.036365107603613E-5
22-23	0.0	0.0	0.0	0.0012079093434719536	7.036365107603613E-5
24-25	0.0	0.0	0.0	0.0018998185790529757	9.381820143471484E-5
26-27	0.0	0.0	0.0	0.003037364271448893	9.381820143471484E-5
28-29	0.0	0.0	0.0	0.007341274262266436	9.381820143471484E-5
30-31	0.0	0.0	0.0	0.018517367508176844	1.055454766140542E-4
32-33	0.0	0.0	0.0	0.040916463100715006	1.876364028694297E-4
34-35	0.0	0.0	0.0	0.07812710724475878	1.876364028694297E-4
36-37	0.0	0.0	0.0	0.13562593744905965	1.876364028694297E-4
38-39	0.0	0.0	0.0	0.21289695360572666	2.345455035867871E-4
40-41	0.0	0.0	0.0	0.2948588798341294	2.345455035867871E-4
42-43	0.0	0.0	0.0	0.3746160783288164	2.345455035867871E-4
44-45	0.0	0.0	0.0	0.4719993714180504	2.4627277876612647E-4
46-47	0.0	0.0	0.0	0.5847336677170396	2.697273291248052E-4
48-49	0.0	0.0	0.0	0.704938238305268	2.8145460430414453E-4
50-51	0.0	0.0	0.0	0.8315810829669537	2.8145460430414453E-4
52-53	0.0	0.0	0.0	0.9510468352188837	3.0490915466282326E-4
54-55	0.0	0.0	0.0	1.0743474064544576	3.0490915466282326E-4
56-57	0.0	0.0	0.0	1.2180651637772615	3.1663642984216265E-4
58-59	0.0	0.0	0.0	1.3785998337072378	3.28363705021502E-4
60-61	0.0	0.0	0.0	1.5470386871080892	3.5181825538018065E-4
62-63	0.0	0.0	0.0	1.7134487219029144	3.752728057388594E-4
64-65	0.0	0.0	0.0	1.8805037568326037	3.752728057388594E-4
66-67	0.0	0.0	0.0	2.0691838871929944	3.752728057388594E-4
68-69	0.0	0.0	0.0	2.271643565889109	3.752728057388594E-4
70-71	0.0	0.0	0.0	2.4904041570845052	3.752728057388594E-4
72-73	0.0	0.0	0.0	2.709340657407592	3.8700008091819877E-4
74-75	0.0	0.0	0.0	2.9272568847900757	3.987273560975381E-4
76-77	0.0	0.0	0.0	3.1640657524864757	3.987273560975381E-4
78-79	0.0	0.0	0.0	3.421057260766518	3.987273560975381E-4
80-81	0.0	0.0	0.0	3.69350531773293	3.987273560975381E-4
82-83	0.0	0.0	0.0	3.972098466893316	3.987273560975381E-4
84-85	0.0	0.0	0.0	4.245273614920847	3.987273560975381E-4
86-87	0.0	0.0	0.0	4.530093947201461	4.1045463127687744E-4
88	0.0	0.0	0.0	4.750179720492123	4.456364568148955E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6340	0.0	37.077705	1
GTATCAA	9605	0.0	32.599354	1
ATCAACG	9980	0.0	29.760508	3
CAACGCA	10065	0.0	29.46214	5
TATCAAC	11595	0.0	26.669136	2
AACGCAG	11705	0.0	26.21653	6
GTACATG	15735	0.0	25.845293	1
TCAACGC	11805	0.0	25.398226	4
TACATGG	15865	0.0	24.763954	2
ACGCAGA	12205	0.0	24.255243	7
CGCAGAG	12235	0.0	24.08281	8
GTGATCG	1270	0.0	24.052113	8
TGATCGC	1275	0.0	23.95779	9
ACATGGG	15935	0.0	23.711432	3
GTACTAG	2045	0.0	22.989994	1
CATGGGG	11710	0.0	21.591066	4
CTGTGCG	2660	0.0	21.553698	9
GCAGAGT	13520	0.0	20.64683	9
GCATCGT	2000	0.0	20.207476	8
GTATAAG	2390	0.0	19.868067	1
>>END_MODULE