FastQCFastQC Report
Mon 16 Jan 2017
ERR1146499_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146499_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4263565
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179230.42037590607859854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160010.37529626028921803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86690.20332749705938574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104900.058.630841
GTATCAA192650.042.1108741
ATCAACG234600.033.728323
CAACGCA237700.033.2291075
TATCAAC245000.032.996882
TCAACGC253000.031.3311024
AACGCAG255800.031.1613456
ACGCAGA276500.028.4868417
CGCAGAG278500.028.2147678
GCAGAGT301300.025.7053479
GTACTAG20450.025.3119371
GTACATG151950.024.8060631
TACATGG153600.023.9263042
GAGTACT208700.023.29557612-13
ACATGGG154150.023.2712463
CAGAGTA290800.023.15886110-11
TACCCCG3800.022.2703725
AGAGTAC283750.021.63908810-11
GTGGTAT34900.021.57351
GTACTTT226500.021.19510514-15
Produced by FastQC (version 0.11.5)