##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146499_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4263565
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.522543692895496	34.0	31.0	34.0	31.0	34.0
2	32.55488329602105	34.0	31.0	34.0	31.0	34.0
3	32.68276477548718	34.0	31.0	34.0	31.0	34.0
4	36.16644967298493	37.0	35.0	37.0	35.0	37.0
5	36.19561493726494	37.0	37.0	37.0	35.0	37.0
6	36.28005061491967	37.0	37.0	37.0	35.0	37.0
7	36.26170423108361	37.0	37.0	37.0	35.0	37.0
8	36.27008618374529	37.0	37.0	37.0	35.0	37.0
9	38.06998626736077	39.0	38.0	39.0	35.0	39.0
10-11	38.04323072358461	39.0	38.0	39.0	36.0	39.0
12-13	38.0220955937109	39.0	38.0	39.0	35.0	39.0
14-15	39.45084618154057	41.0	39.0	41.0	36.5	41.0
16-17	39.4473663471766	41.0	39.0	41.0	36.5	41.0
18-19	39.51492377857497	41.0	39.5	41.0	37.0	41.0
20-21	39.55224981910678	41.0	40.0	41.0	37.0	41.0
22-23	39.50788483346683	41.0	39.0	41.0	36.5	41.0
24-25	39.49253981116742	41.0	39.0	41.0	36.0	41.0
26-27	39.41170147517394	41.0	39.0	41.0	36.0	41.0
28-29	39.320979860750334	41.0	39.0	41.0	36.0	41.0
30-31	39.200104959112856	41.0	39.0	41.0	35.5	41.0
32-33	39.05277613921683	41.0	39.0	41.0	35.0	41.0
34-35	38.96034726807261	41.0	39.0	41.0	35.0	41.0
36-37	38.864045933391424	41.0	38.0	41.0	35.0	41.0
38-39	38.75164000079745	40.0	38.0	41.0	35.0	41.0
40-41	38.65203216087946	40.0	38.0	41.0	35.0	41.0
42-43	38.54048020846405	40.0	38.0	41.0	35.0	41.0
44-45	38.36317612139137	40.0	38.0	41.0	34.5	41.0
46-47	38.09504546078223	40.0	38.0	41.0	33.5	41.0
48-49	37.84049475028526	40.0	38.0	41.0	33.0	41.0
50-51	37.670185161947806	40.0	37.0	41.0	33.0	41.0
52-53	37.53326066331814	40.0	37.0	41.0	33.0	41.0
54-55	37.39149409003967	40.0	36.5	41.0	33.0	41.0
56-57	37.18553252970226	40.0	36.0	41.0	32.5	41.0
58-59	36.97273056702548	39.0	35.5	41.0	32.0	41.0
60-61	36.79403292784325	39.0	35.0	41.0	32.0	41.0
62-63	36.53756340058143	39.0	35.0	41.0	31.5	41.0
64-65	36.24436099836639	38.0	35.0	41.0	31.0	41.0
66-67	35.9868139690611	37.0	35.0	40.0	31.0	41.0
68-69	35.68358462929497	37.0	35.0	40.0	31.0	41.0
70-71	35.412595328088116	36.0	35.0	39.0	31.0	41.0
72-73	35.10195341222662	36.0	35.0	39.0	30.5	41.0
74-75	34.77970008666456	35.5	35.0	39.0	30.0	40.5
76-77	34.48054093229492	35.0	35.0	37.0	30.0	39.5
78-79	34.198991688880085	35.0	35.0	37.0	30.0	39.0
80-81	33.94560514499017	35.0	34.5	36.5	30.0	39.0
82-83	33.69297489307657	35.0	34.0	36.0	29.0	37.5
84-85	33.48874240219159	35.0	34.0	36.0	29.0	37.0
86-87	33.30667399230456	35.0	34.0	35.5	29.0	37.0
88-89	33.155987067160936	35.0	34.0	35.0	29.0	36.0
90-91	33.06902850079687	35.0	34.0	35.0	29.0	36.0
92-93	32.98289471369617	35.0	34.0	35.0	29.0	36.0
94-95	32.90999328027132	35.0	34.0	35.0	29.0	36.0
96-97	32.866034058352575	35.0	34.0	35.0	29.0	35.5
98-99	32.81623817157707	35.0	34.0	35.0	29.0	35.0
100	32.26252842398321	35.0	33.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2780551899156052
1101	2	0.25018719025415237
1101	3	0.27235671403703066
1101	4	0.15107643770272716
1101	5	0.15376142495372136
1101	6	0.14913839846892785
1101	7	0.19271408825974135
1101	8	0.19013620468449233
1101	9	0.22280358053181004
1101	10-11	0.23083161931162266
1101	12-13	0.2413568977063818
1101	14-15	0.35330300878271714
1101	16-17	0.3177455375952789
1101	18-19	0.315041435077525
1101	20-21	0.30811911465828246
1101	22-23	0.32293773598333786
1101	24-25	0.33373591720077656
1101	26-27	0.32950492997294134
1101	28-29	0.3713940559846378
1101	30-31	0.31588632166737796
1101	32-33	0.2971759516103063
1101	34-35	0.3425882089031518
1101	36-37	0.3458203771906554
1101	38-39	0.3836361041074454
1101	40-41	0.32891950283590177
1101	42-43	0.32109994914024753
1101	44-45	0.3302824627295493
1101	46-47	0.37854396526208944
1101	48-49	0.38028201452492993
1101	50-51	0.3913691530024508
1101	52-53	0.3447225327040968
1101	54-55	0.33139449882007455
1101	56-57	0.3414897236402652
1101	58-59	0.31999223828383094
1101	60-61	0.3284318896061649
1101	62-63	0.29696785987685104
1101	64-65	0.27571474182590094
1101	66-67	0.24737079920303273
1101	68-69	0.272153721151426
1101	70-71	0.24754648929113898
1101	72-73	0.25541876107011774
1101	74-75	0.2534006725237248
1101	76-77	0.2104238117806645
1101	78-79	0.2132605187641019
1101	80-81	0.2867842821464208
1101	82-83	0.2613607720216251
1101	84-85	0.2786216656335512
1101	86-87	0.2405491956664676
1101	88-89	0.24956764808099052
1101	90-91	0.23639009563485303
1101	92-93	0.22791906092338365
1101	94-95	0.2138591256834772
1101	96-97	0.18886830336014526
1101	98-99	0.23912475209788653
1101	100	0.23103177541554487
1102	1	0.25845162345535755
1102	2	0.24592416806898854
1102	3	0.2558855263338131
1102	4	0.18553613534336932
1102	5	0.1550479654777206
1102	6	0.16282753446665765
1102	7	0.1818267377771008
1102	8	0.15969088322800218
1102	9	0.20036042273174814
1102	10-11	0.19933043517918492
1102	12-13	0.2352578003308281
1102	14-15	0.3037835582052111
1102	16-17	0.28125699660306935
1102	18-19	0.29314666946013546
1102	20-21	0.30237294088817634
1102	22-23	0.2959766782677633
1102	24-25	0.2717609014500795
1102	26-27	0.27355703512382235
1102	28-29	0.3180116083150608
1102	30-31	0.3095708794212797
1102	32-33	0.28947140496133983
1102	34-35	0.3262808118196432
1102	36-37	0.26565977306188415
1102	38-39	0.2686735765346384
1102	40-41	0.29770113535372644
1102	42-43	0.25699041029444913
1102	44-45	0.31520302791415844
1102	46-47	0.3164019910959226
1102	48-49	0.254610052342386
1102	50-51	0.2082130708242147
1102	52-53	0.25543665149706385
1102	54-55	0.2596456906902418
1102	56-57	0.22128397478685002
1102	58-59	0.21399613062198597
1102	60-61	0.19082512709965727
1102	62-63	0.20347197811518924
1102	64-65	0.15184323011430223
1102	66-67	0.1953879460140584
1102	68-69	0.16042161821073364
1102	70-71	0.10830047037950408
1102	72-73	0.15093042032413706
1102	74-75	0.13623595566131286
1102	76-77	0.11056351676644027
1102	78-79	0.06454954159362813
1102	80-81	0.06551302182277396
1102	82-83	0.10150600870582593
1102	84-85	0.06719336988481928
1102	86-87	0.078400948226097
1102	88-89	0.0824756658862924
1102	90-91	0.11379141464269082
1102	92-93	0.11598048001694394
1102	94-95	0.12188825195556063
1102	96-97	0.05213221730577544
1102	98-99	0.11141864959382985
1102	100	0.061941900791708804
1103	1	0.19614077937471563
1103	2	0.16591023409630168
1103	3	0.20013731456431572
1103	4	0.1578955691133217
1103	5	0.10267123745229156
1103	6	0.06553950670624431
1103	7	0.11708864941958552
1103	8	0.10528566448479637
1103	9	0.152010433596665
1103	10-11	0.1472818947740251
1103	12-13	0.13274453281423604
1103	14-15	0.24842009881646732
1103	16-17	0.23235592108854775
1103	18-19	0.23515602896782895
1103	20-21	0.23685862720353157
1103	22-23	0.24035951752750861
1103	24-25	0.24786425030791293
1103	26-27	0.23012481986858546
1103	28-29	0.24992667828735193
1103	30-31	0.2528873889387171
1103	32-33	0.2527507945547782
1103	34-35	0.2762372401526463
1103	36-37	0.2766848609332797
1103	38-39	0.3540897251576993
1103	40-41	0.3109283285543114
1103	42-43	0.3055498570574926
1103	44-45	0.3505207202511471
1103	46-47	0.3574769679659866
1103	48-49	0.3757773697838189
1103	50-51	0.3742788069165641
1103	52-53	0.41432594361988606
1103	54-55	0.33404459210178317
1103	56-57	0.31917853359291826
1103	58-59	0.28672945185829946
1103	60-61	0.28944046656662437
1103	62-63	0.32169680791686517
1103	64-65	0.29850949354703715
1103	66-67	0.27572950755988046
1103	68-69	0.257355696182735
1103	70-71	0.2914370031501079
1103	72-73	0.33914994087898265
1103	74-75	0.25176807884479757
1103	76-77	0.26666265406984024
1103	78-79	0.26426682980060434
1103	80-81	0.22296915333080136
1103	82-83	0.2802226494983131
1103	84-85	0.28665960016275704
1103	86-87	0.2568935701651611
1103	88-89	0.25583601589335103
1103	90-91	0.2684585114255569
1103	92-93	0.23167949724623327
1103	94-95	0.1969345678535035
1103	96-97	0.23820675733906427
1103	98-99	0.23520250724329372
1103	100	0.32304259502613775
1104	1	0.1541044250415311
1104	2	0.16474232648527476
1104	3	0.17564422410701752
1104	4	0.12696358459234602
1104	5	0.10638576634111985
1104	6	0.07601903574027347
1104	7	0.08548199892152297
1104	8	0.09797026754264948
1104	9	0.13524946555026673
1104	10-11	0.12497062843674911
1104	12-13	0.14583026614636196
1104	14-15	0.20648920936739046
1104	16-17	0.20400179524506967
1104	18-19	0.1837179441383725
1104	20-21	0.20570309424199706
1104	22-23	0.22854743504358055
1104	24-25	0.2129271517606952
1104	26-27	0.1885671090974057
1104	28-29	0.29099345156942746
1104	30-31	0.2762944694427887
1104	32-33	0.1950704102469203
1104	34-35	0.26397789068294486
1104	36-37	0.2730266830763668
1104	38-39	0.2907260361853403
1104	40-41	0.23850907205204663
1104	42-43	0.29940420794942924
1104	44-45	0.2662257002689543
1104	46-47	0.3043523109781461
1104	48-49	0.3134252622474918
1104	50-51	0.28348419643812406
1104	52-53	0.28103164466492103
1104	54-55	0.286925062315575
1104	56-57	0.3040864932132905
1104	58-59	0.32846965399273387
1104	60-61	0.345402005224436
1104	62-63	0.33605101163735185
1104	64-65	0.3007678492962995
1104	66-67	0.32998947392183453
1104	68-69	0.3484813148494865
1104	70-71	0.3056438962421453
1104	72-73	0.3140097104700743
1104	74-75	0.3229473652199033
1104	76-77	0.21258683372546727
1104	78-79	0.26117831695846405
1104	80-81	0.2432104934808237
1104	82-83	0.25658165963398005
1104	84-85	0.22247560470324856
1104	86-87	0.2858437581725539
1104	88-89	0.2765592779859247
1104	90-91	0.26149011250898724
1104	92-93	0.2260533632087771
1104	94-95	0.22480885940242246
1104	96-97	0.17614736898034522
1104	98-99	0.19270127236084278
1104	100	0.17008390450782684
1105	1	0.1846737235489968
1105	2	0.17661072908578035
1105	3	0.18314976764802537
1105	4	0.11982035957564818
1105	5	0.09559511140511745
1105	6	0.099383807651229
1105	7	0.09960802860573637
1105	8	0.06480600023070338
1105	9	0.09848862300580663
1105	10-11	0.13373307963377812
1105	12-13	0.11622032187561615
1105	14-15	0.2075514134067049
1105	16-17	0.18465662733716925
1105	18-19	0.14418262081365185
1105	20-21	0.15289343052241833
1105	22-23	0.1781754735583192
1105	24-25	0.1557843786917914
1105	26-27	0.1613828151196941
1105	28-29	0.19340247694161405
1105	30-31	0.18616242155975016
1105	32-33	0.16728539176168766
1105	34-35	0.19677266180742237
1105	36-37	0.19548049436341586
1105	38-39	0.19549621425361607
1105	40-41	0.18024653847662364
1105	42-43	0.21752143228260934
1105	44-45	0.22971475247416606
1105	46-47	0.27469563727620283
1105	48-49	0.29363946635574223
1105	50-51	0.2568442109297422
1105	52-53	0.22374006672762192
1105	54-55	0.24402281237301793
1105	56-57	0.2456128253955967
1105	58-59	0.3173500064027621
1105	60-61	0.2269010814346899
1105	62-63	0.23038594032294668
1105	64-65	0.2627738369147252
1105	66-67	0.1773582971998522
1105	68-69	0.23130659737810788
1105	70-71	0.219697434062752
1105	72-73	0.18923738445300842
1105	74-75	0.17516246274869474
1105	76-77	0.10150012059931868
1105	78-79	0.14027445290052754
1105	80-81	0.15584008599201837
1105	82-83	0.13006388587871243
1105	84-85	0.16648120142203737
1105	86-87	0.13742922624587806
1105	88-89	0.10202714744009
1105	90-91	0.13821391673466366
1105	92-93	0.11531105964746757
1105	94-95	0.087075796892357
1105	96-97	0.0489847178111944
1105	98-99	0.09211612245313461
1105	100	0.17607977345152648
1106	1	0.13771425734531562
1106	2	0.1106557054067494
1106	3	0.12063455073254659
1106	4	0.11619352047338083
1106	5	0.08799727179020067
1106	6	0.048967885245936316
1106	7	0.05759577314059072
1106	8	0.03961969972790058
1106	9	0.0810571816538257
1106	10-11	0.08970535218541187
1106	12-13	0.08650867398625905
1106	14-15	0.10948180073698666
1106	16-17	0.08922804547140828
1106	18-19	0.1162128656132424
1106	20-21	0.09300835929307993
1106	22-23	0.13661253384919547
1106	24-25	0.10382386491532714
1106	26-27	0.06259978224436225
1106	28-29	0.1500355514501166
1106	30-31	0.1638461774779998
1106	32-33	0.0845828133524904
1106	34-35	0.1433477368483338
1106	36-37	0.12407596994982129
1106	38-39	0.13077535755459024
1106	40-41	0.09061275518948264
1106	42-43	0.10294205336195716
1106	44-45	0.0964202687424276
1106	46-47	0.15217765293932928
1106	48-49	0.229033923445634
1106	50-51	0.20653928934216026
1106	52-53	0.2512078057714504
1106	54-55	0.19655730457296272
1106	56-57	0.1833043572586348
1106	58-59	0.21469933371206196
1106	60-61	0.18616268597664032
1106	62-63	0.16801993851927932
1106	64-65	0.21012453479043813
1106	66-67	0.13841365962101548
1106	68-69	0.1840737983356462
1106	70-71	0.13540829838793655
1106	72-73	0.06694118267999727
1106	74-75	0.14712676701071103
1106	76-77	0.16065943515619097
1106	78-79	0.1659886336951928
1106	80-81	0.14097564295090592
1106	82-83	0.13406570702053955
1106	84-85	0.13075153473310763
1106	86-87	0.13357048621236345
1106	88-89	0.1315935645815145
1106	90-91	0.06899179682798717
1106	92-93	0.04069412474274259
1106	94-95	0.09244376193313286
1106	96-97	0.049334287550557576
1106	98-99	0.06751189506770316
1106	100	0.09576206274049781
1107	1	0.07577893167405847
1107	2	0.0566432463968809
1107	3	0.08279235946370278
1107	4	0.04211044235920269
1107	5	-0.015864503672126773
1107	6	0.016796112847693223
1107	7	0.06084018378400913
1107	8	0.06284804001817434
1107	9	0.04419855138770146
1107	10-11	0.030983046912801626
1107	12-13	0.07295042209381819
1107	14-15	0.10489337935744913
1107	16-17	0.07505317818432644
1107	18-19	0.013831241973385033
1107	20-21	0.03322954732710315
1107	22-23	0.08140594895638742
1107	24-25	0.0794856246942004
1107	26-27	0.11818643415281826
1107	28-29	0.13603367410343026
1107	30-31	0.11821727359993162
1107	32-33	0.12274720519236126
1107	34-35	0.1329425773449131
1107	36-37	0.12608206720340576
1107	38-39	0.13750383901668783
1107	40-41	0.14626537717864352
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2216	28-29	-0.37332125606161526
2216	30-31	-0.4224594525894929
2216	32-33	-0.4406461503518315
2216	34-35	-0.473925015361921
2216	36-37	-0.44779776791913406
2216	38-39	-0.3960700385415308
2216	40-41	-0.4027321226303968
2216	42-43	-0.468611263722714
2216	44-45	-0.3994455444026386
2216	46-47	-0.36534828421851273
2216	48-49	-0.3998473411017045
2216	50-51	-0.3835236100165318
2216	52-53	-0.3622555285190643
2216	54-55	-0.4056123935428033
2216	56-57	-0.3723006718885671
2216	58-59	-0.36467082058017297
2216	60-61	-0.3428126328683092
2216	62-63	-0.3326725263978574
2216	64-65	-0.3417144120348823
2216	66-67	-0.3475856738570613
2216	68-69	-0.3600040785060443
2216	70-71	-0.30272422624769746
2216	72-73	-0.3047377638651767
2216	74-75	-0.317283063428917
2216	76-77	-0.3036488995195228
2216	78-79	-0.39023447488835217
2216	80-81	-0.32990160962472004
2216	82-83	-0.27732020333635177
2216	84-85	-0.3155826822378742
2216	86-87	-0.2726428755094332
2216	88-89	-0.2769525363235985
2216	90-91	-0.28832797961285905
2216	92-93	-0.32842714606125867
2216	94-95	-0.35969433183750965
2216	96-97	-0.31338787669631785
2216	98-99	-0.35955155058216093
2216	100	-0.45641142906396936
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	26.0
8	96.0
9	191.0
10	352.0
11	371.0
12	304.0
13	342.0
14	586.0
15	947.0
16	1812.0
17	3533.0
18	6235.0
19	10108.0
20	14538.0
21	21061.0
22	31088.0
23	36881.0
24	35720.0
25	29984.0
26	25088.0
27	25262.0
28	29630.0
29	35520.0
30	42597.0
31	53618.0
32	69579.0
33	92361.0
34	138742.0
35	223080.0
36	402890.0
37	987813.0
38	1400209.0
39	541670.0
40	1329.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.91696895533493	20.14010713274595	13.572907804712257	23.37001610720687
2	15.371265740387106	21.14521168052158	40.03628185196131	23.44724072713
3	17.453800790750048	27.26818773195182	31.377887941659843	23.900123535638286
4	12.029416697059855	18.40844457631114	39.52441208237707	30.037726644251933
5	12.318844320265269	38.59846051400981	35.43239208792611	13.650303077798807
6	28.39808432695542	39.37077299161834	18.41604669370951	13.815095987716724
7	24.784399909465435	34.33380281524968	23.426475261899373	17.455322013385512
8	25.61576990147916	34.335210088271204	21.01215766617842	19.036862344071217
9	26.513914998364047	14.520946672561577	21.18902843043322	37.77610989864116
10-11	24.136655592209806	26.31233486530638	28.951816144470648	20.599193398013167
12-13	25.48847032940743	24.570482682919106	28.624718985168514	21.316328002504946
14-15	22.675084196011646	25.269205591661247	27.284222140027016	24.771488072300095
16-17	21.094928774394198	28.14269279347213	28.020306949700547	22.74207148243313
18-19	20.81210207889407	26.812737697208792	31.124106235040394	21.251053988856743
20-21	22.19725745942656	25.976066976814003	30.684556234043576	21.14211932971586
22-23	22.142041087443513	25.839108853415503	30.458051183277	21.56079887586398
24-25	21.749850184059582	26.15540046885646	30.78497689140426	21.309772455679695
26-27	21.55304305199991	26.42804788950092	30.346200890569275	21.672708167929887
28-29	21.25886259919227	26.620739508246977	30.584912196676818	21.535485695883935
30-31	21.78526864873486	26.151385227368678	30.48642449033202	21.57692163356444
32-33	21.91299817954113	26.43777022344746	29.94443161586922	21.70479998114219
34-35	21.961189853825445	26.490439717523063	30.307234774446385	21.24113565420511
36-37	22.073533293874586	26.161195977647132	30.0899173911162	21.675353337362083
38-39	21.947544837877185	26.186523505069864	30.212016952161907	21.653914704891044
40-41	21.960237077824306	26.014399690207174	30.30779094472856	21.71757228723996
42-43	22.008294293923104	26.00657412449087	29.843942823481978	22.141188758104047
44-45	21.99019522877311	26.58181769993056	29.633298553911757	21.794688517384568
46-47	22.36810750107657	26.481817490972308	28.803721689072106	22.346353318879018
48-49	22.62038048956341	26.575128353043315	28.579357943358485	22.22513321403479
50-51	22.479978421611225	26.830955605983313	28.2297277487525	22.459338223652964
52-53	22.286299584884237	27.031005794639366	28.205531147520606	22.477163472955787
54-55	22.155311728185126	27.07723015651497	28.141933017316802	22.625525097983108
56-57	22.716460073042878	26.859219476475204	27.876840350104832	22.547480100377083
58-59	22.131184646392434	26.87942673535297	28.448874981499685	22.540513636754913
60-61	22.28799793408129	27.166303289672978	28.158427443694155	22.387271332551578
62-63	22.359397881300424	26.817964487723323	28.060848434441894	22.76178919653436
64-65	21.95952911329817	26.887507297646152	28.15223716687374	23.000726422181934
66-67	22.271824390420107	26.843047624453586	28.018843793982995	22.86628419114331
68-69	22.150183885207873	26.59042614762161	28.322860650793785	22.93652931637673
70-71	22.178771893160743	26.47639058057849	28.12820673396835	23.216630792292417
72-73	22.390852339649545	26.557865467732295	28.113968819997176	22.93731337262098
74-75	22.44470758852996	27.003649550716723	27.82033313071734	22.73130973003598
76-77	22.390773183556124	26.8835265008037	27.64552414634427	23.080176169295907
78-79	22.224218484026537	26.946344509102737	27.680842457924186	23.148594548946537
80-81	22.259873317193467	27.02645112232448	27.735576496835275	22.97809906364678
82-83	22.586081333535045	26.57344633762201	28.186982459473377	22.653489869369576
84-85	22.759566475066265	26.9258373458243	27.424364281407023	22.890231897702414
86-87	22.082056476935318	27.12727276138845	27.743846198139188	23.04682456353705
88-89	22.520227246927213	26.688613917584288	27.552915922764942	23.238242912723557
90-91	22.371152119312374	26.748076638690467	27.63923238407675	23.24153885792041
92-93	22.559454353340456	26.69132521727709	27.623960230464412	23.125260198918042
94-95	21.93286202961443	26.71747471334718	27.94931985884578	23.400343398192618
96-97	22.247877705553076	26.58083201166353	27.789204103814054	23.382086178969335
98-99	22.037448905458312	26.880194110883142	27.509225016520887	23.573131967137655
100	22.289990824500272	26.516865844436815	27.287002688852986	23.906140642209923
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	787.0
1	516.0
2	586.0
3	883.0
4	559.5
5	421.5
6	918.5
7	2315.5
8	4455.5
9	5440.0
10	3900.5
11	2276.5
12	2189.0
13	2513.0
14	2987.0
15	3556.5
16	4142.5
17	4895.5
18	5787.5
19	6593.5
20	7348.5
21	8414.5
22	10160.0
23	12402.0
24	14883.0
25	18165.0
26	22813.5
27	28459.5
28	35590.5
29	43893.5
30	52316.5
31	61720.5
32	73081.5
33	82400.5
34	90872.5
35	99958.5
36	109324.5
37	124762.0
38	130851.0
39	128434.0
40	127593.5
41	124122.5
42	124969.0
43	132697.5
44	143098.5
45	157030.0
46	168694.5
47	173931.5
48	172453.0
49	165168.0
50	161049.0
51	160996.0
52	157657.5
53	152069.5
54	146365.5
55	133136.0
56	119750.5
57	105190.5
58	90192.0
59	80776.5
60	65438.5
61	48678.0
62	38700.0
63	30226.0
64	21115.5
65	13699.0
66	9694.5
67	7127.0
68	5188.5
69	3661.5
70	2532.0
71	2032.5
72	1615.5
73	1370.5
74	1142.0
75	875.5
76	652.5
77	472.0
78	367.0
79	262.5
80	185.0
81	123.5
82	89.0
83	64.0
84	48.0
85	29.0
86	16.5
87	14.0
88	9.0
89	6.0
90	3.5
91	2.0
92	1.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00600436489182175
2	0.0840611084855045
3	4.2218190645621677E-4
4	0.0
5	0.038066735232135544
6	0.004855091924246493
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	4.690910071735742E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.629092086082891E-4
24-25	0.0
26-27	0.0
28-29	4.690910071735742E-5
30-31	1.1727275179339355E-4
32-33	0.0018646367535149577
34-35	0.0015597275988521342
36-37	9.499092895264878E-4
38-39	8.912729136297911E-4
40-41	0.0016535458002868491
42-43	7.388183362983795E-4
44-45	6.684546852223434E-4
46-47	3.987273560975381E-4
48-49	7.388183362983795E-4
50-51	9.381820143471484E-4
52-53	0.0013017275449066685
54-55	0.0017590912769009034
56-57	9.850911150645059E-4
58-59	0.0029083642444761603
60-61	0.0020288186060257087
62-63	0.001254818444189311
64-65	0.004784728273170457
66-67	0.004655728246197724
68-69	0.0040224553865134
70-71	0.0017825458272595822
72-73	0.005242092005164692
74-75	0.003834818983643969
76-77	0.003893455359540666
78-79	9.499092895264878E-4
80-81	7.036365107603613E-5
82-83	2.345455035867871E-4
84-85	9.381820143471484E-5
86-87	3.5181825538018066E-5
88-89	2.1109095322810839E-4
90-91	1.1727275179339355E-5
92-93	0.0
94-95	3.5181825538018066E-5
96-97	4.4563645681489555E-4
98-99	5.27727383070271E-4
100	8.678183632711122E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4263565.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.898105741037725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.5353328323638	31.869164075197
2	15.904570395287227	15.235975891184914
3	6.83595831760883	9.822883630144622
4	3.5430672615394205	6.788248413632965
5	2.0723761970842043	4.963144711157443
6	1.3038476201587217	3.7471098708337705
7	0.8739963829632614	2.9303939817921156
8	0.6060705632454799	2.322370553988983
9	0.4525542123311313	1.9508840564209706
>10	1.8032918978483081	13.752322390482421
>50	0.04514889537442586	1.4776549841518674
>100	0.02097386156389846	1.8585836865580823
>500	0.0012697379624655861	0.4207305344936162
>1k	0.0012697379624649124	1.0102311388379157
>5k	9.069556874749376E-5	0.3765940117216036
>10k+	1.8139113749498752E-4	1.4737080694017395
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17923	0.42037590607859854	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16001	0.37529626028921803	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8669	0.20332749705938574	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.345455035867871E-5
2	0.0	0.0	0.0	2.345455035867871E-5	2.345455035867871E-5
3	0.0	0.0	0.0	2.345455035867871E-5	2.345455035867871E-5
4	0.0	0.0	0.0	2.345455035867871E-5	2.345455035867871E-5
5	0.0	0.0	0.0	2.345455035867871E-5	2.345455035867871E-5
6	0.0	0.0	0.0	2.345455035867871E-5	2.345455035867871E-5
7	0.0	0.0	0.0	2.345455035867871E-5	2.345455035867871E-5
8	0.0	0.0	0.0	2.345455035867871E-5	2.345455035867871E-5
9	0.0	0.0	0.0	2.345455035867871E-5	2.345455035867871E-5
10-11	0.0	0.0	0.0	4.690910071735742E-5	2.345455035867871E-5
12-13	0.0	0.0	0.0	1.1727275179339355E-4	4.690910071735742E-5
14-15	0.0	0.0	0.0	1.7590912769009035E-4	4.690910071735742E-5
16-17	0.0	2.345455035867871E-5	0.0	2.110909532281084E-4	4.690910071735742E-5
18-19	0.0	2.345455035867871E-5	0.0	2.2281822840744775E-4	7.036365107603613E-5
20-21	0.0	2.345455035867871E-5	0.0	4.3390918163555616E-4	1.1727275179339355E-4
22-23	0.0	2.345455035867871E-5	0.0	9.499092895264877E-4	1.1727275179339355E-4
24-25	0.0	2.345455035867871E-5	0.0	0.0016535458002868491	1.1727275179339355E-4
26-27	0.0	2.345455035867871E-5	0.0	0.0028262733182207846	1.1727275179339355E-4
28-29	0.0	2.345455035867871E-5	0.0	0.007118456033858989	1.1727275179339355E-4
30-31	0.0	2.345455035867871E-5	0.0	0.01838836748120411	1.1727275179339355E-4
32-33	0.0	2.345455035867871E-5	0.0	0.04130346318163321	1.1727275179339355E-4
34-35	0.0	2.345455035867871E-5	0.0	0.07871347100372575	1.4072730215207227E-4
36-37	0.0	2.345455035867871E-5	0.0	0.13642339216125474	1.4072730215207227E-4
38-39	0.0	2.345455035867871E-5	0.0	0.21409313567401927	1.4072730215207227E-4
40-41	0.0	2.345455035867871E-5	0.0	0.2963599710570849	1.4072730215207227E-4
42-43	0.0	2.345455035867871E-5	0.0	0.3763517150553586	1.4072730215207227E-4
44-45	0.0	2.345455035867871E-5	0.0	0.47452073558160834	1.4072730215207227E-4
46-47	0.0	2.345455035867871E-5	0.0	0.5877358501629505	1.4072730215207227E-4
48-49	0.0	2.345455035867871E-5	0.0	0.7088551482151673	1.4072730215207227E-4
50-51	0.0	2.345455035867871E-5	0.0	0.8362837203138688	1.64181852510751E-4
52-53	0.0	2.345455035867871E-5	0.0	0.9563006544992277	1.64181852510751E-4
54-55	0.0	2.345455035867871E-5	0.0	1.0805863168498662	1.64181852510751E-4
56-57	0.0	2.345455035867871E-5	0.0	1.2252774380125553	1.7590912769009035E-4
58-59	0.0	2.345455035867871E-5	0.0	1.3866447444802648	1.9936367804876905E-4
60-61	0.0	2.345455035867871E-5	0.0	1.5562211435735118	2.110909532281084E-4
62-63	0.0	2.345455035867871E-5	0.0	1.723803905886271	2.345455035867871E-4
64-65	0.0	2.345455035867871E-5	0.0	1.891468759125286	2.345455035867871E-4
66-67	0.0	2.345455035867871E-5	0.0	2.0815444352320185	2.580000539454658E-4
68-69	0.0	2.345455035867871E-5	0.0	2.284848477741045	2.580000539454658E-4
70-71	0.0	2.345455035867871E-5	0.0	2.505309523837446	2.580000539454658E-4
72-73	0.0	2.345455035867871E-5	0.0	2.726274842766558	2.8145460430414453E-4
74-75	0.0	2.345455035867871E-5	0.0	2.945891525050046	2.8145460430414453E-4
76-77	0.0	2.345455035867871E-5	0.0	3.184717484077292	3.0490915466282326E-4
78-79	0.0	2.345455035867871E-5	0.0	3.4431162653788556	3.0490915466282326E-4
80-81	0.0	2.345455035867871E-5	0.0	3.71765177732719	3.0490915466282326E-4
82-83	0.0	2.345455035867871E-5	0.0	3.99864901789934	3.28363705021502E-4
84-85	0.0	2.345455035867871E-5	0.0	4.274146166412381	3.28363705021502E-4
86-87	0.0	3.5181825538018066E-5	0.0	4.561229862802607	3.28363705021502E-4
88	0.0	4.690910071735742E-5	0.0	4.783391363800012	3.28363705021502E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10490	0.0	58.63084	1
GTATCAA	19265	0.0	42.110874	1
ATCAACG	23460	0.0	33.72832	3
CAACGCA	23770	0.0	33.229107	5
TATCAAC	24500	0.0	32.99688	2
TCAACGC	25300	0.0	31.331102	4
AACGCAG	25580	0.0	31.161345	6
ACGCAGA	27650	0.0	28.486841	7
CGCAGAG	27850	0.0	28.214767	8
GCAGAGT	30130	0.0	25.705347	9
GTACTAG	2045	0.0	25.311937	1
GTACATG	15195	0.0	24.806063	1
TACATGG	15360	0.0	23.926304	2
GAGTACT	20870	0.0	23.295576	12-13
ACATGGG	15415	0.0	23.271246	3
CAGAGTA	29080	0.0	23.158861	10-11
TACCCCG	380	0.0	22.270372	5
AGAGTAC	28375	0.0	21.639088	10-11
GTGGTAT	3490	0.0	21.5735	1
GTACTTT	22650	0.0	21.195105	14-15
>>END_MODULE