FastQCFastQC Report
Mon 16 Jan 2017
ERR1146498_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146498_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375077
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14900.036.6057051
GTATCAA25300.034.567621
ATCAACG26800.030.337723
CAACGCA27800.029.0773815
TATCAAC31850.026.8555222
GTACTAG5550.026.2630841
TCAACGC31150.026.2520284
AACGCAG32650.025.3329266
ATTGCGT2301.546141E-1024.5193676
GCATCGT4300.024.0433128
ACGCAGA34100.023.7035887
GTACATG46300.023.662031
TACATGG43950.023.418382
CGCAGAG34650.023.3273418
CTCGTCG2253.1850504E-922.9747189
TAGTACT6500.022.414034
ACATGGG44900.022.1901973
TTGCGTG2355.2641553E-921.997077
CTGTGCG5150.021.8999989
GGGCCGT1100.00306156221.361576
Produced by FastQC (version 0.11.5)