##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146498_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1375077 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.945093983827814 31.0 31.0 34.0 27.0 34.0 2 30.986386216917307 31.0 31.0 34.0 27.0 34.0 3 31.118617357427983 31.0 31.0 34.0 27.0 34.0 4 34.72665385283879 37.0 35.0 37.0 32.0 37.0 5 34.672856138238075 37.0 35.0 37.0 31.0 37.0 6 34.85722035929624 37.0 35.0 37.0 32.0 37.0 7 34.70780981719569 37.0 35.0 37.0 31.0 37.0 8 34.691978703738044 37.0 35.0 37.0 31.0 37.0 9 36.2570481507581 39.0 37.0 39.0 31.0 39.0 10-11 36.26421756745259 39.0 37.0 39.0 31.0 39.0 12-13 36.228278852747884 39.0 37.0 39.0 31.0 39.0 14-15 37.53744881195744 40.0 38.0 41.0 31.0 41.0 16-17 37.53083936390471 40.0 38.0 41.0 31.0 41.0 18-19 37.54872963477682 40.0 38.0 41.0 31.0 41.0 20-21 37.69685115815332 40.0 38.0 41.0 31.0 41.0 22-23 37.708381785165486 40.0 38.0 41.0 31.0 41.0 24-25 37.77356795292191 40.0 38.0 41.0 31.0 41.0 26-27 37.828375792773784 40.0 38.0 41.0 31.5 41.0 28-29 37.79275815099809 40.0 38.0 41.0 31.0 41.0 30-31 37.77918582013953 40.0 38.0 41.0 31.0 41.0 32-33 37.807422784324075 40.0 38.0 41.0 31.0 41.0 34-35 37.74750504880818 40.0 38.0 41.0 31.0 41.0 36-37 37.64558966516057 40.0 38.0 41.0 30.5 41.0 38-39 37.602965143042894 40.0 38.0 41.0 30.0 41.0 40-41 37.555212908077145 40.0 37.0 41.0 30.0 41.0 42-43 37.41862710233681 40.0 37.0 41.0 30.0 41.0 44-45 37.259639641998234 40.0 37.0 41.0 30.0 41.0 46-47 37.16215201039651 40.0 37.0 41.0 30.0 41.0 48-49 37.06260522138033 40.0 36.0 41.0 30.0 41.0 50-51 36.95976843478583 40.0 36.0 41.0 30.0 41.0 52-53 36.87684507849379 40.0 36.0 41.0 30.0 41.0 54-55 36.73544826944237 39.0 35.0 41.0 30.0 41.0 56-57 36.63956491163768 39.0 35.0 41.0 29.5 41.0 58-59 36.46860066745353 39.0 35.0 41.0 29.0 41.0 60-61 36.32815216893309 39.0 35.0 40.5 29.0 41.0 62-63 36.14357523251425 38.5 35.0 40.0 29.0 41.0 64-65 35.93315210711836 38.0 35.0 40.0 29.0 41.0 66-67 35.71219393532144 37.5 34.5 40.0 28.5 41.0 68-69 35.45317134967715 37.0 34.0 40.0 28.0 41.0 70-71 35.20814579838074 37.0 34.0 39.5 28.0 41.0 72-73 34.90179131786802 36.0 34.0 39.0 28.0 41.0 74-75 34.591775587839805 36.0 34.0 39.0 28.0 40.5 76-77 34.26879658375495 35.0 34.0 38.5 28.0 40.0 78-79 33.96930317356774 35.0 34.0 37.0 27.5 39.0 80-81 33.65551965453571 35.0 33.0 37.0 27.0 39.0 82-83 33.368613903075975 35.0 33.0 36.5 27.0 38.5 84-85 33.10961931586377 35.0 33.0 36.0 26.5 37.0 86-87 32.8820480598541 35.0 33.0 36.0 26.0 37.0 88-89 32.69755148257153 35.0 33.0 35.0 26.0 36.5 90-91 32.51319671552939 35.0 33.0 35.0 26.0 36.0 92-93 32.40442498856427 35.0 33.0 35.0 26.0 36.0 94-95 32.29259052402156 35.0 33.0 35.0 25.5 36.0 96-97 32.213233877084704 35.0 33.0 35.0 25.0 35.5 98-99 32.13301473299313 35.0 33.0 35.0 25.0 35.0 100 31.21037367361973 34.0 31.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38668800521106306 1101 2 0.3774978491379919 1101 3 0.46548553545147797 1101 4 0.49634505640921134 1101 5 0.3435363229089745 1101 6 0.41806065143646975 1101 7 0.37074873113878937 1101 8 0.4881928834771614 1101 9 0.49672429278446373 1101 10-11 0.6261469365111552 1101 12-13 0.592039401424401 1101 14-15 0.6899409915135735 1101 16-17 0.6529257095120471 1101 18-19 0.6570319045261428 1101 20-21 0.6715287085734403 1101 22-23 0.651287374896306 1101 24-25 0.6648564112155739 1101 26-27 0.6664390158315356 1101 28-29 0.6417313453849474 1101 30-31 0.7840437001451122 1101 32-33 0.7243920532586188 1101 34-35 0.7225423015073531 1101 36-37 0.7842251011176131 1101 38-39 0.6790163572691128 1101 40-41 0.6412389132547034 1101 42-43 0.6004068866144081 1101 44-45 0.6902501097594325 1101 46-47 0.6374424957400464 1101 48-49 0.6611873069741989 1101 50-51 0.6968278234367702 1101 52-53 0.6762138255676291 1101 54-55 0.6500428846590154 1101 56-57 0.5957597723489059 1101 58-59 0.5354483129439274 1101 60-61 0.5805736194249178 1101 62-63 0.5243504820799245 1101 64-65 0.5252802645696377 1101 66-67 0.5525864138180054 1101 68-69 0.5862759755636802 1101 70-71 0.5068687784384736 1101 72-73 0.5040166965760378 1101 74-75 0.4894334901054478 1101 76-77 0.46791066670005677 1101 78-79 0.5964162067479251 1101 80-81 0.48788354875425455 1101 82-83 0.5373670814185232 1101 84-85 0.5127380993529087 1101 86-87 0.5087370340987007 1101 88-89 0.4221128086272472 1101 90-91 0.49562385150744603 1101 92-93 0.5350401984634061 1101 94-95 0.3949804381674511 1101 96-97 0.43785075280693775 1101 98-99 0.4451039648216195 1101 100 0.5373475181819884 1102 1 0.3960152558165859 1102 2 0.33070519600858717 1102 3 0.405955787023462 1102 4 0.4435727598010928 1102 5 0.3626026239756257 1102 6 0.24565646584726863 1102 7 0.3399743507008637 1102 8 0.33216943184623204 1102 9 0.41593251485068095 1102 10-11 0.5156649371954458 1102 12-13 0.46534016268979883 1102 14-15 0.5091071629132529 1102 16-17 0.48818416148641575 1102 18-19 0.4982274333535841 1102 20-21 0.5111620099925531 1102 22-23 0.4388741084269725 1102 24-25 0.4505835959073252 1102 26-27 0.4124745957260316 1102 28-29 0.45008011180735963 1102 30-31 0.5415890376323418 1102 32-33 0.4282549100403017 1102 34-35 0.47717666225498334 1102 36-37 0.4443281768465468 1102 38-39 0.416301754562447 1102 40-41 0.35697834230081327 1102 42-43 0.3397572489627976 1102 44-45 0.29502221546268714 1102 46-47 0.3745215093939436 1102 48-49 0.3294152873716172 1102 50-51 0.32040421972690325 1102 52-53 0.24916608870935164 1102 54-55 0.313933745120778 1102 56-57 0.21973845549874937 1102 58-59 0.3939998996632639 1102 60-61 0.32135899301638204 1102 62-63 0.33573050820680095 1102 64-65 0.17920473009243665 1102 66-67 0.21842856709591274 1102 68-69 0.2605880811572874 1102 70-71 0.3040729895676435 1102 72-73 0.4395459948297358 1102 74-75 0.25816224105553687 1102 76-77 0.26110926161444326 1102 78-79 0.19474237093707103 1102 80-81 0.1465408103464867 1102 82-83 0.07622460608539683 1102 84-85 0.1755123920777919 1102 86-87 0.2647823817438848 1102 88-89 0.08155723031931927 1102 90-91 0.10561036885124508 1102 92-93 0.1200476847074583 1102 94-95 0.13294593404768307 1102 96-97 0.1445020460422981 1102 98-99 0.1039948591432065 1102 100 0.017455843794746073 1103 1 0.43243277027085725 1103 2 0.4633898578281652 1103 3 0.37390867182977416 1103 4 0.5057178710273362 1103 5 0.3339764117994335 1103 6 0.41724848257599234 1103 7 0.3837071257200577 1103 8 0.5243208234597461 1103 9 0.5139181986723145 1103 10-11 0.5311675007706285 1103 12-13 0.5435549157140542 1103 14-15 0.677592295988255 1103 16-17 0.4666080235947305 1103 18-19 0.5572671162166571 1103 20-21 0.5106623602324802 1103 22-23 0.5765714377424089 1103 24-25 0.5654831517526446 1103 26-27 0.5488518148015871 1103 28-29 0.5234487472557845 1103 30-31 0.4496203334942024 1103 32-33 0.4680860510592524 1103 34-35 0.5928948998916397 1103 36-37 0.6283627661926445 1103 38-39 0.5408101089004802 1103 40-41 0.45931243432562496 1103 42-43 0.3818516902056075 1103 44-45 0.3342862782587943 1103 46-47 0.30598961298216665 1103 48-49 0.43031219911568286 1103 50-51 0.45572619144510185 1103 52-53 0.5453864403981044 1103 54-55 0.4960837331873904 1103 56-57 0.4620160369739992 1103 58-59 0.35031159185572847 1103 60-61 0.3605090580517043 1103 62-63 0.4597092984787352 1103 64-65 0.47436385156263583 1103 66-67 0.40658033514458936 1103 68-69 0.2623632122466262 1103 70-71 0.3337804826323776 1103 72-73 0.3197637774455515 1103 74-75 0.33659577276114305 1103 76-77 0.25796583399768025 1103 78-79 0.3481865909433637 1103 80-81 0.34291189779780495 1103 82-83 0.3551195619189471 1103 84-85 0.35397903972866374 1103 86-87 0.41374042604034145 1103 88-89 0.3827548374335592 1103 90-91 0.4158291604685118 1103 92-93 0.37574061969218775 1103 94-95 0.3234688555208507 1103 96-97 0.22336343317351037 1103 98-99 0.2075043408463202 1103 100 0.2623945049144929 1104 1 0.2603224683487717 1104 2 0.15346335246767495 1104 3 0.13554563217209292 1104 4 0.2562777816147275 1104 5 0.22735107620853512 1104 6 0.2344277077450485 1104 7 0.21707507676566706 1104 8 0.24749163281568087 1104 9 0.3153862369421958 1104 10-11 0.2963118226312744 1104 12-13 0.2042033549188602 1104 14-15 0.34112327791348207 1104 16-17 0.25963799449730374 1104 18-19 0.23137736373948314 1104 20-21 0.28788161831309367 1104 22-23 0.25045744285223037 1104 24-25 0.33275522657141465 1104 26-27 0.20910925930970592 1104 28-29 0.2674393287653487 1104 30-31 0.28880637835258227 1104 32-33 0.18338272176259096 1104 34-35 0.27015860464212693 1104 36-37 0.24416411368709845 1104 38-39 0.2561806513291245 1104 40-41 0.17109865264346524 1104 42-43 0.27217505054693447 1104 44-45 0.2033435452275043 1104 46-47 0.24166341275208225 1104 48-49 0.12292607748017303 1104 50-51 0.050769738672627795 1104 52-53 0.11186527506700372 1104 54-55 0.1669768516189123 1104 56-57 0.08374566769573732 1104 58-59 0.16085240706576087 1104 60-61 0.08178655672417534 1104 62-63 0.06985265907970017 1104 64-65 0.15558850258828016 1104 66-67 0.09435808819542757 1104 68-69 0.058944280437955854 1104 70-71 0.145416082993151 1104 72-73 0.13957750324482987 1104 74-75 0.05491376612072685 1104 76-77 0.04222206935856576 1104 78-79 0.11014623781797894 1104 80-81 0.14198945940033525 1104 82-83 0.1305484351927788 1104 84-85 0.1109807811232244 1104 86-87 -0.001555904667611685 1104 88-89 0.08971559392948336 1104 90-91 0.010388677897609 1104 92-93 0.07486540974503697 1104 94-95 0.05673335524236478 1104 96-97 -0.020057965147152856 1104 98-99 -0.0779837596241677 1104 100 0.03210542847178388 1105 1 0.15547318918756403 1105 2 0.1696711272677227 1105 3 0.1602686535636053 1105 4 0.25378624745925293 1105 5 0.21429668411420266 1105 6 0.2620619721287767 1105 7 0.1425313918389719 1105 8 0.226416293131237 1105 9 0.35016779769685513 1105 10-11 0.3605780683273849 1105 12-13 0.2692374896604264 1105 14-15 0.35723698004322557 1105 16-17 0.3255825188973347 1105 18-19 0.2742152189159981 1105 20-21 0.3698326910616032 1105 22-23 0.3475593644674362 1105 24-25 0.3612023289244277 1105 26-27 0.42469209099171223 1105 28-29 0.4384333846528605 1105 30-31 0.45684424232405973 1105 32-33 0.46193022860025934 1105 34-35 0.437034337149548 1105 36-37 0.3977371913134746 1105 38-39 0.40201012874987185 1105 40-41 0.439331007685972 1105 42-43 0.3215833807653894 1105 44-45 0.25212899190708526 1105 46-47 0.31508518367288474 1105 48-49 0.26209420788604376 1105 50-51 0.2682696489139431 1105 52-53 0.24602584486906665 1105 54-55 0.31520880031201415 1105 56-57 0.3377518970877631 1105 58-59 0.30726455056721136 1105 60-61 0.23103799710757045 1105 62-63 0.14903735518183225 1105 64-65 0.13706063968276538 1105 66-67 0.2843247647610596 1105 68-69 0.31223953753327294 1105 70-71 0.31144710971841505 1105 72-73 0.3398756134139447 1105 74-75 0.30002400509592775 1105 76-77 0.20153330155189764 1105 78-79 0.23838370840982748 1105 80-81 0.32487730668643877 1105 82-83 0.2507124945899264 1105 84-85 0.1631603586144763 1105 86-87 0.2213153323907875 1105 88-89 0.20119226488137087 1105 90-91 0.20154935374016247 1105 92-93 0.20298946432166076 1105 94-95 0.18977507257513793 1105 96-97 0.11330549185092309 1105 98-99 0.1795135956256786 1105 100 0.10369890539489646 1106 1 0.20316482681822734 1106 2 0.18726044704952116 1106 3 0.19168728776026356 1106 4 0.26451672834580364 1106 5 0.21580037179161593 1106 6 0.18093831019591988 1106 7 0.1786041726286669 1106 8 0.29314957489159355 1106 9 0.21852699089425442 1106 10-11 0.10429719791811465 1106 12-13 0.23552506350831948 1106 14-15 0.2511908348086891 1106 16-17 0.05784618468767633 1106 18-19 0.12533297406596233 1106 20-21 0.18990917525218265 1106 22-23 0.2055072631831436 1106 24-25 0.20436243680838118 1106 26-27 0.29481034719619714 1106 28-29 0.17446989664253465 1106 30-31 0.13089268423318146 1106 32-33 0.1383890781874655 1106 34-35 0.19846372619846164 1106 36-37 0.2425008308980381 1106 38-39 0.15161389735073527 1106 40-41 0.29425397317400126 1106 42-43 0.15097903354089937 1106 44-45 0.19282006978227884 1106 46-47 0.07779118731234291 1106 48-49 0.09949245084088432 1106 50-51 0.18489373118751473 1106 52-53 0.2882708731084094 1106 54-55 0.3068367086295183 1106 56-57 0.20100305957618758 1106 58-59 0.19174619499041512 1106 60-61 0.16548227208797073 1106 62-63 0.2868891939890261 1106 64-65 0.18135875978490645 1106 66-67 0.21351884267399157 1106 68-69 0.22120642181273809 1106 70-71 0.30672037311375533 1106 72-73 0.13405882116918377 1106 74-75 0.20258999649204412 1106 76-77 0.26575803498670325 1106 78-79 0.1527407286239182 1106 80-81 0.2289047944535767 1106 82-83 0.20828219148338434 1106 84-85 0.32269237054753575 1106 86-87 0.17911354368904142 1106 88-89 0.09924968279437962 1106 90-91 0.19595638506550017 1106 92-93 0.056101479357486994 1106 94-95 0.14853241543480067 1106 96-97 0.16339797204983597 1106 98-99 0.13501460821703404 1106 100 0.07106170241121035 1107 1 0.09371009623445303 1107 2 0.09245371203399344 1107 3 0.1910552948760369 1107 4 0.05109331652051452 1107 5 0.09231793495188612 1107 6 0.05944352998900371 1107 7 0.11419941629704056 1107 8 0.1034102914622892 1107 9 0.1547044872661516 1107 10-11 0.04253403530088207 1107 12-13 0.15660300149498596 1107 14-15 0.1321918413158798 1107 16-17 0.14514800420299423 1107 18-19 0.07555817029584233 1107 20-21 0.12657578340098752 1107 22-23 0.13724617990052224 1107 24-25 0.14152644676422454 1107 26-27 0.1986669667364751 1107 28-29 0.22590904587087834 1107 30-31 0.18147796035945163 1107 32-33 0.07198544929992323 1107 34-35 0.08696964820585862 1107 36-37 0.1678783686225529 1107 38-39 0.18444826840077866 1107 40-41 0.10053157409780766 1107 42-43 0.18710796008927844 1107 44-45 0.16618859185521728 1107 46-47 0.1922574849263725 1107 48-49 0.11094195773038251 1107 50-51 0.05981521972581305 1107 52-53 0.20844958665178126 1107 54-55 0.1187685977420756 1107 56-57 0.08146180058393782 1107 58-59 0.11618939755584279 1107 60-61 0.10240635881924476 1107 62-63 0.006984617112166802 1107 64-65 1.3375673681537137E-5 1107 66-67 -0.007532372156774159 1107 68-69 0.11393538269160786 1107 70-71 0.1425358389939433 1107 72-73 0.04729536596264694 1107 74-75 0.014874943357824577 1107 76-77 0.0894754210329225 1107 78-79 0.05377386143604923 1107 80-81 -0.025347990996536396 1107 82-83 0.017260391630067318 1107 84-85 0.06469346678210997 1107 86-87 -0.03960866032761601 1107 88-89 -1.5850658500937698E-4 1107 90-91 0.045582553472101495 1107 92-93 -0.051510426296118794 1107 94-95 -0.08485436421035075 1107 96-97 -0.031171473044807385 1107 98-99 -0.0521898765917328 1107 100 -0.00577809909621152 1108 1 0.1613919408690272 1108 2 0.22977112639360442 1108 3 0.2179492876683966 1108 4 0.29586289985837055 1108 5 0.3440041750160887 1108 6 0.2608037274941637 1108 7 0.2409692454319412 1108 8 0.25725032818726135 1108 9 0.4652112268044135 1108 10-11 0.4486156146192286 1108 12-13 0.3433491184437827 1108 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90-91 -0.17183755362229647 2214 92-93 -0.24905167169631426 2214 94-95 -0.2193213984536584 2214 96-97 -0.17858328215761077 2214 98-99 -0.13972517739738777 2214 100 -0.12246789212040454 2215 1 -0.31281660980200954 2215 2 -0.24511122429698773 2215 3 -0.2860467503024644 2215 4 -0.3541436893531795 2215 5 -0.37201781169495973 2215 6 -0.49765278158407256 2215 7 -0.4864520772566294 2215 8 -0.28863183970585027 2215 9 -0.5011423460361328 2215 10-11 -0.4291183767850413 2215 12-13 -0.4908726956724365 2215 14-15 -0.52302620899831 2215 16-17 -0.5221091732744796 2215 18-19 -0.6103711359378963 2215 20-21 -0.576929659191471 2215 22-23 -0.42833626486839194 2215 24-25 -0.47677790803186326 2215 26-27 -0.5007785560686884 2215 28-29 -0.4361900530720888 2215 30-31 -0.4451737007252561 2215 32-33 -0.5091802706719406 2215 34-35 -0.5856234290071711 2215 36-37 -0.53345566949654 2215 38-39 -0.5491284543165733 2215 40-41 -0.5967389336512312 2215 42-43 -0.5378687155460682 2215 44-45 -0.40480972705744733 2215 46-47 -0.31232596936148127 2215 48-49 -0.3960060672086314 2215 50-51 -0.43787129936911384 2215 52-53 -0.47742610242793404 2215 54-55 -0.3632303003080608 2215 56-57 -0.31341842916466334 2215 58-59 -0.27985759201246196 2215 60-61 -0.30290412241367193 2215 62-63 -0.36810998015620555 2215 64-65 -0.38616805755436445 2215 66-67 -0.36950250108346694 2215 68-69 -0.3927775933095745 2215 70-71 -0.33534843894005206 2215 72-73 -0.28612805141060704 2215 74-75 -0.26416533485923566 2215 76-77 -0.3201061990788858 2215 78-79 -0.3169486811652078 2215 80-81 -0.21472775064726335 2215 82-83 -0.2131836089533934 2215 84-85 -0.1822702136437684 2215 86-87 -0.1923375748853431 2215 88-89 -0.26361713436136824 2215 90-91 -0.3186629647115211 2215 92-93 -0.32881852743287254 2215 94-95 -0.3386301311342308 2215 96-97 -0.38243983466344744 2215 98-99 -0.42024654541030415 2215 100 -0.33258169350356326 2216 1 -0.1668914993407462 2216 2 -0.12163098181710907 2216 3 -0.184467810168794 2216 4 -0.24089915534043627 2216 5 -0.10932619659082832 2216 6 -0.15369138042187558 2216 7 -0.22413997196925095 2216 8 -0.04261624454181856 2216 9 -0.18824546692166422 2216 10-11 -0.256795818542237 2216 12-13 -0.25295261267035585 2216 14-15 -0.4541296382729243 2216 16-17 -0.41357220453592447 2216 18-19 -0.440687814467978 2216 20-21 -0.42014578674501735 2216 22-23 -0.38141809375907343 2216 24-25 -0.5597758084759832 2216 26-27 -0.5297751978550522 2216 28-29 -0.4379806222232645 2216 30-31 -0.44969315463752224 2216 32-33 -0.42895309071675314 2216 34-35 -0.4380242917794064 2216 36-37 -0.3627936908220022 2216 38-39 -0.5046754534696944 2216 40-41 -0.42352153907278023 2216 42-43 -0.379641958729394 2216 44-45 -0.249395974697471 2216 46-47 -0.3000289548187851 2216 48-49 -0.20042598197132122 2216 50-51 -0.27736076005279386 2216 52-53 -0.14239782446419014 2216 54-55 -0.08487972169006497 2216 56-57 -0.10283410955404548 2216 58-59 -0.08232258297893225 2216 60-61 -0.06748043705659512 2216 62-63 -0.03773771987343366 2216 64-65 -0.09717058502998555 2216 66-67 -0.05240068308203405 2216 68-69 -0.0802337778553408 2216 70-71 -0.11428053417490247 2216 72-73 -0.10359309317760079 2216 74-75 -0.15472695559923721 2216 76-77 -0.0778922762681944 2216 78-79 -0.149879793858112 2216 80-81 -0.08475938443403663 2216 82-83 -0.010957171283550338 2216 84-85 -0.06956730289311963 2216 86-87 0.04163993900859708 2216 88-89 -0.07463170455007173 2216 90-91 -0.03768719417212196 2216 92-93 0.014001953666038958 2216 94-95 -0.06331787224996788 2216 96-97 0.0013184002513355608 2216 98-99 -0.07231941607634695 2216 100 -0.13786088423324117 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 43.0 1 19.0 2 29.0 3 21.0 4 15.0 5 20.0 6 65.0 7 315.0 8 994.0 9 1307.0 10 1582.0 11 2003.0 12 2587.0 13 3147.0 14 3843.0 15 4100.0 16 4467.0 17 4744.0 18 5329.0 19 5759.0 20 6348.0 21 6879.0 22 7961.0 23 9106.0 24 10740.0 25 13390.0 26 15142.0 27 13892.0 28 14485.0 29 17427.0 30 20211.0 31 21828.0 32 27381.0 33 36926.0 34 54161.0 35 82044.0 36 149200.0 37 305275.0 38 401322.0 39 120772.0 40 198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.05606195340687 22.102919966176536 14.090586918586459 23.750431161830136 2 14.135431091664435 22.22894580661678 41.22594055834196 22.409682543376817 3 17.299595244762365 27.76293634832952 31.32929184198181 23.608176564926303 4 11.245825235939627 18.637266824153702 41.35954755449013 28.757360385416547 5 11.889356477770779 39.839348549933014 36.21705401548619 12.054240956810013 6 27.857686270915977 40.32079283965595 19.53769930453427 12.283821584893802 7 24.592154946212162 34.213312853809946 24.201473129352628 16.993059070625264 8 24.32472137938658 36.21922518596526 21.939246015237952 17.516807419410206 9 25.550050764965484 15.964054331604318 21.503326632551833 36.98256827087836 10-11 22.92454487172261 27.692068805889875 30.314672434552186 19.06871388783533 12-13 23.764113443558408 25.771896133346473 29.341889613225554 21.122100809869565 14-15 21.249816808789106 27.208158672862865 27.037313549531085 24.504710968816948 16-17 20.879520499574163 29.893609206571092 27.79543118891057 21.431439104944175 18-19 20.619839594458718 29.705717106590956 29.06551317227322 20.6089301266771 20-21 21.964330195694824 28.95668822467358 28.35736714734295 20.721614432288646 22-23 21.720239222914607 28.94910713214313 27.884880063096553 21.445773581845707 24-25 21.33040925571655 29.046876028393758 28.4123036711089 21.210411044780784 26-27 21.38030394875933 29.258877172133996 27.928842259444806 21.431976619661867 28-29 21.068404103053435 29.467423431390806 28.134054703500276 21.330117762055483 30-31 21.649243475803846 28.98385710789457 28.068216819721886 21.298682596579706 32-33 21.455336505382835 29.320887087741447 27.689635660391904 21.53414074648381 34-35 21.68374304768125 29.294072809605925 28.028199848570917 20.993984294141903 36-37 21.625366594511554 29.02362934385401 27.965851430300493 21.38515263133394 38-39 21.454533552031016 29.074523296031074 28.212037343477803 21.25890580846011 40-41 21.582894725357214 28.790867643678663 28.30428919322318 21.321948437740943 42-43 21.35565220617279 28.8364245285009 28.124602189114654 21.683321076211655 44-45 21.461121943962816 29.1673350057268 28.061547571374064 21.309995478936315 46-47 21.37683571123296 28.922934228114684 27.834319957079597 21.865910103572762 48-49 21.50325454171797 28.74141352048843 28.134517420300558 21.62081451749305 50-51 21.652710153372514 28.727683946263237 27.872615625911614 21.74699027445263 52-53 21.398205213261868 28.958940761562236 27.90179740559038 21.74105661958552 54-55 21.453930506357384 28.841767772998004 28.032880746546308 21.6714209740983 56-57 21.757633276089294 28.75103353923484 27.759381517026434 21.73195166764943 58-59 21.533269943305452 28.740672462949934 28.190205003932174 21.53585258981244 60-61 21.61350389749563 28.887992425082924 27.925995417364323 21.572508260057116 62-63 21.527487796538654 28.66984090032809 27.89925869883094 21.90341260430231 64-65 21.2015504439921 28.729519109193102 27.986998153609772 22.08193229320502 66-67 21.54845252597857 28.61078181058262 27.79313031607152 22.047635347367294 68-69 21.44111359240571 28.61932628183279 27.914600622114193 22.02495950364731 70-71 21.391831143103047 28.313228030582465 28.006428458661357 22.288512367653123 72-73 21.620707328455918 28.380038361185143 28.088128148037946 21.911126162320993 74-75 21.547722805123453 28.67900726205529 27.820212114289706 21.953057818531555 76-77 21.357490324920438 28.361778679576716 27.98237805980416 22.298352935698688 78-79 21.41374584046898 28.439151899452476 28.014359566812217 22.132742693266323 80-81 21.3549851580234 28.506999010534894 28.01514027122985 22.122875560211863 82-83 21.659098655509442 28.21869642508324 28.413198495055237 21.709006424352083 84-85 21.826212654264403 28.41003995378979 27.833721813372442 21.93002557857336 86-87 21.267938737488258 28.608898966906228 28.06689029389448 22.056272001711033 88-89 21.712955907220497 28.171613493176917 27.952464489677446 22.16296610992514 90-91 21.65636488565223 28.185170891469923 28.013230025742025 22.14523419713582 92-93 21.778154297649387 28.130544887627245 28.191472722153797 21.899828092569567 94-95 21.32891922005368 28.28860472721413 28.219963107402858 22.162512945329333 96-97 21.337552742616033 28.241779426742326 28.16350210970464 22.257165720937 98-99 21.23852603674869 28.45051846527876 27.98610393748445 22.324851560488103 100 21.40284590489257 28.250054376409878 27.82857354854995 22.518526170147606 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1266.0 1 977.5 2 741.5 3 735.0 4 593.0 5 456.5 6 377.5 7 357.5 8 370.0 9 378.0 10 332.0 11 262.5 12 233.0 13 254.5 14 284.5 15 312.5 16 381.5 17 440.0 18 527.0 19 724.5 20 998.5 21 1374.0 22 2016.0 23 2941.0 24 3908.5 25 5263.0 26 7361.0 27 10035.5 28 13103.5 29 16989.0 30 21404.5 31 25463.0 32 30115.5 33 34520.5 34 38375.5 35 42327.5 36 45884.0 37 49756.5 38 51299.5 39 50379.0 40 49648.5 41 49843.5 42 50349.5 43 50442.0 44 51389.5 45 53922.0 46 55054.0 47 54592.5 48 54270.0 49 52209.5 50 48898.5 51 46597.5 52 43837.5 53 40255.5 54 37195.5 55 33060.0 56 28503.0 57 23749.0 58 19524.5 59 16741.0 60 13659.0 61 10447.0 62 7840.5 63 5677.0 64 3898.0 65 2593.5 66 1909.5 67 1433.0 68 1035.5 69 789.5 70 665.0 71 524.5 72 359.5 73 279.0 74 225.5 75 172.5 76 122.5 77 102.5 78 82.5 79 52.0 80 44.5 81 37.0 82 28.0 83 22.0 84 15.0 85 15.5 86 15.5 87 6.5 88 3.5 89 6.0 90 7.0 91 7.0 92 4.0 93 1.5 94 3.0 95 4.0 96 3.0 97 2.0 98 2.5 99 3.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06421458580137694 2 0.010835756833980933 3 0.012508390439226313 4 0.010835756833980933 5 0.012726560039910491 6 0.012435667238998253 7 0.003272544010262698 8 0.0064723648202973365 9 0.008217721625770776 10-11 0.007817744024516446 12-13 0.007454128023376146 14-15 0.010726672033638843 16-17 0.010617587233296753 18-19 0.009054038428393463 20-21 0.007599574423832266 22-23 0.003018012809464488 24-25 0.004108860812885388 26-27 0.005490601617218527 28-29 0.0073450432230340555 30-31 0.009999440031358244 32-33 0.010981203234437053 34-35 0.012799283240138553 36-37 0.018980755259523648 38-39 0.019780710462032308 40-41 0.019853433662260368 42-43 0.026616691283469947 44-45 0.029125641691338012 46-47 0.03196184650023235 48-49 0.033416310504793545 50-51 0.03272544010262698 52-53 0.03119825289783772 54-55 0.038834188921784016 56-57 0.04017956812600313 58-59 0.037670617718135055 60-61 0.03930688972326641 62-63 0.033961734506504 64-65 0.036579769714714154 66-67 0.03857965772098581 68-69 0.041488585730108204 70-71 0.04101588492862582 72-73 0.03712519371642461 74-75 0.038906912122012076 76-77 0.03930688972326641 78-79 0.038034233719275355 80-81 0.04341575053615179 82-83 0.03937961292349446 84-85 0.03556164491152132 86-87 0.03403445770673206 88-89 0.03210729290068847 90-91 0.034652604908670566 92-93 0.03636160011402998 94-95 0.03974322892463476 96-97 0.0346889665087846 98-99 0.03221637770103056 100 0.029089280091223982 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1375077.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.27221659155315 #Duplication Level Percentage of deduplicated Percentage of total 1 74.57756253435706 49.424203771470104 2 14.843498298418476 19.6742306841828 3 5.31896561406234 10.574989236544887 4 2.328925060567496 6.173721041577005 5 1.1532194151723958 3.8213203429944635 6 0.6219702364671255 2.473160773478932 7 0.38461383503789 1.7842447965817256 8 0.2182020857833211 1.1568588711808734 9 0.14487232156876567 0.864090888581374 >10 0.4030777887341134 3.7518631313083874 >50 0.0039300757930330645 0.17031968177718002 >100 0.0011627340379916213 0.13099678032227932 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 7.272320022805995E-5 0.0 6 1.454464004561199E-4 0.0 0.0 7.272320022805995E-5 0.0 7 1.454464004561199E-4 0.0 0.0 7.272320022805995E-5 0.0 8 1.454464004561199E-4 0.0 0.0 7.272320022805995E-5 0.0 9 1.454464004561199E-4 0.0 0.0 7.272320022805995E-5 0.0 10-11 1.454464004561199E-4 0.0 0.0 1.0908480034208993E-4 0.0 12-13 1.454464004561199E-4 0.0 0.0 1.454464004561199E-4 1.8180800057014988E-4 14-15 1.454464004561199E-4 0.0 0.0 1.454464004561199E-4 2.1816960068417988E-4 16-17 2.1816960068417988E-4 0.0 0.0 1.454464004561199E-4 2.1816960068417988E-4 18-19 2.1816960068417988E-4 0.0 0.0 1.8180800057014988E-4 2.1816960068417988E-4 20-21 2.1816960068417988E-4 0.0 0.0 3.2725440102626983E-4 2.1816960068417988E-4 22-23 2.1816960068417988E-4 0.0 0.0 6.908704021665696E-4 2.1816960068417988E-4 24-25 2.1816960068417988E-4 0.0 0.0 0.001490825604675229 2.1816960068417988E-4 26-27 2.908928009122398E-4 0.0 0.0 0.0025816736080961286 2.1816960068417988E-4 28-29 2.908928009122398E-4 0.0 0.0 0.006981427221893755 2.1816960068417988E-4 30-31 2.908928009122398E-4 0.0 0.0 0.015562764848804832 2.1816960068417988E-4 32-33 2.908928009122398E-4 0.0 0.0 0.03570709131197744 2.1816960068417988E-4 34-35 2.908928009122398E-4 0.0 0.0 0.06988699541916563 2.908928009122398E-4 36-37 2.908928009122398E-4 0.0 0.0 0.1216659139815443 2.908928009122398E-4 38-39 2.908928009122398E-4 0.0 0.0 0.19482545341097263 2.908928009122398E-4 40-41 2.908928009122398E-4 0.0 0.0 0.2694030952448481 2.908928009122398E-4 42-43 2.908928009122398E-4 0.0 0.0 0.34118089386994327 3.2725440102626983E-4 44-45 2.908928009122398E-4 0.0 0.0 0.43004864454863256 4.363392013683597E-4 46-47 2.908928009122398E-4 0.0 0.0 0.531242977665978 5.090624015964197E-4 48-49 2.908928009122398E-4 0.0 0.0 0.6433094292174184 5.090624015964197E-4 50-51 2.908928009122398E-4 0.0 0.0 0.7601028887836827 5.090624015964197E-4 52-53 2.908928009122398E-4 0.0 0.0 0.8681695643225797 5.090624015964197E-4 54-55 2.908928009122398E-4 0.0 0.0 0.9806723550753884 6.181472019385096E-4 56-57 2.908928009122398E-4 0.0 0.0 1.1122649858880629 6.545088020525396E-4 58-59 2.908928009122398E-4 0.0 0.0 1.257493216743499 6.545088020525396E-4 60-61 2.908928009122398E-4 0.0 0.0 1.409339258819688 6.908704021665696E-4 62-63 2.908928009122398E-4 0.0 0.0 1.558458180887325 7.999552025086596E-4 64-65 2.908928009122398E-4 0.0 0.0 1.715249400579022 7.999552025086596E-4 66-67 2.908928009122398E-4 0.0 0.0 1.8820036987019635 7.999552025086596E-4 68-69 2.908928009122398E-4 0.0 0.0 2.0710840192949194 7.999552025086596E-4 70-71 3.2725440102626983E-4 0.0 0.0 2.274454448732689 8.363168026226896E-4 72-73 3.636160011402998E-4 0.0 0.0 2.478261217371827 9.817632030788095E-4 74-75 3.636160011402998E-4 0.0 0.0 2.6790136116013867 0.0010908480034208994 76-77 3.636160011402998E-4 0.0 0.0 2.8947469850779264 0.0010908480034208994 78-79 3.636160011402998E-4 0.0 0.0 3.128188457809999 0.0010908480034208994 80-81 3.636160011402998E-4 0.0 0.0 3.383155997809577 0.0010908480034208994 82-83 3.636160011402998E-4 0.0 0.0 3.6455776658325316 0.001309017604105079 84-85 3.636160011402998E-4 0.0 0.0 3.901926946636443 0.001309017604105079 86-87 3.636160011402998E-4 0.0 0.0 4.171330041881291 0.0013817408043331392 88 3.636160011402998E-4 0.0 0.0 4.379100224932858 0.0014544640045611992 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1490 0.0 36.605705 1 GTATCAA 2530 0.0 34.56762 1 ATCAACG 2680 0.0 30.33772 3 CAACGCA 2780 0.0 29.077381 5 TATCAAC 3185 0.0 26.855522 2 GTACTAG 555 0.0 26.263084 1 TCAACGC 3115 0.0 26.252028 4 AACGCAG 3265 0.0 25.332926 6 ATTGCGT 230 1.546141E-10 24.519367 6 GCATCGT 430 0.0 24.043312 8 ACGCAGA 3410 0.0 23.703588 7 GTACATG 4630 0.0 23.66203 1 TACATGG 4395 0.0 23.41838 2 CGCAGAG 3465 0.0 23.327341 8 CTCGTCG 225 3.1850504E-9 22.974718 9 TAGTACT 650 0.0 22.41403 4 ACATGGG 4490 0.0 22.190197 3 TTGCGTG 235 5.2641553E-9 21.99707 7 CTGTGCG 515 0.0 21.899998 9 GGGCCGT 110 0.003061562 21.36157 6 >>END_MODULE