FastQCFastQC Report
Mon 16 Jan 2017
ERR1146498_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146498_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375077
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59700.4341575053615179No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54270.3946688076376814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31500.2290780807183889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37050.057.9175871
GTATCAA63900.038.5890661
ATCAACG80350.030.0768073
CAACGCA81150.029.7803025
TATCAAC83850.029.3473722
TCAACGC83400.029.0332494
AACGCAG85400.028.5070726
ACGCAGA92350.026.1571967
CGCAGAG93150.025.9325478
TACATGG48250.023.988792
GCAGAGT100650.023.6266239
CATCGTG1000.001759629923.4982199
ACATGGG47250.023.4835993
GAGTACT71350.023.44881612-13
GTACATG50500.023.3892421
AGAGTAC93050.022.32393810-11
ACTGCGC3600.022.1927628
CAGAGTA98800.022.19011910-11
GTACTTT74450.022.15681514-15
CTGCGCA3750.021.305059
Produced by FastQC (version 0.11.5)