##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146498_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1375077
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43234306151583	34.0	31.0	34.0	31.0	34.0
2	32.461012728741736	34.0	31.0	34.0	31.0	34.0
3	32.604203255526784	34.0	31.0	34.0	31.0	34.0
4	36.127402320015534	37.0	35.0	37.0	35.0	37.0
5	36.17009374747742	37.0	35.0	37.0	35.0	37.0
6	36.26984161614222	37.0	37.0	37.0	35.0	37.0
7	36.245743329282654	37.0	37.0	37.0	35.0	37.0
8	36.2552926127046	37.0	37.0	37.0	35.0	37.0
9	38.057625136628715	39.0	38.0	39.0	35.0	39.0
10-11	38.02672068545979	39.0	38.0	39.0	35.0	39.0
12-13	38.019505816765175	39.0	38.0	39.0	35.0	39.0
14-15	39.44095639735084	41.0	39.0	41.0	36.5	41.0
16-17	39.44084222192648	41.0	39.0	41.0	36.5	41.0
18-19	39.514317016428905	41.0	39.5	41.0	37.0	41.0
20-21	39.553176294854765	41.0	40.0	41.0	37.0	41.0
22-23	39.51278510221609	41.0	39.0	41.0	37.0	41.0
24-25	39.51202841731772	41.0	39.0	41.0	36.5	41.0
26-27	39.43675990508168	41.0	39.0	41.0	36.0	41.0
28-29	39.33626407830253	41.0	39.0	41.0	36.0	41.0
30-31	39.21296807378787	41.0	39.0	41.0	35.5	41.0
32-33	39.06799364690123	41.0	39.0	41.0	35.0	41.0
34-35	38.98229517328848	41.0	39.0	41.0	35.0	41.0
36-37	38.89041268234433	41.0	39.0	41.0	35.0	41.0
38-39	38.784451707068044	40.0	38.0	41.0	35.0	41.0
40-41	38.69261975874805	40.0	38.0	41.0	35.0	41.0
42-43	38.59832103947633	40.0	38.0	41.0	35.0	41.0
44-45	38.44057750947765	40.0	38.0	41.0	34.5	41.0
46-47	38.18457148217881	40.0	38.0	41.0	34.0	41.0
48-49	37.97297351348324	40.0	38.0	41.0	33.0	41.0
50-51	37.84956296992823	40.0	38.0	41.0	33.0	41.0
52-53	37.74949730087842	40.0	37.5	41.0	33.0	41.0
54-55	37.64341742316976	40.0	37.0	41.0	33.0	41.0
56-57	37.48964785244753	40.0	37.0	41.0	33.0	41.0
58-59	37.328857220359296	40.0	36.5	41.0	32.5	41.0
60-61	37.18846217339102	40.0	36.0	41.0	32.0	41.0
62-63	36.97025184771471	39.0	35.5	41.0	32.0	41.0
64-65	36.716785314567844	39.0	35.0	41.0	31.5	41.0
66-67	36.492350973800015	39.0	35.0	41.0	31.0	41.0
68-69	36.20455218144147	38.0	35.0	40.5	31.0	41.0
70-71	35.93904086825683	37.0	35.0	40.0	31.0	41.0
72-73	35.613748902788714	37.0	35.0	39.5	31.0	41.0
74-75	35.284249900187405	36.0	35.0	39.0	31.0	41.0
76-77	34.947873464540535	36.0	35.0	39.0	30.5	40.5
78-79	34.6236941640359	35.5	35.0	37.5	30.0	39.5
80-81	34.310690237710325	35.0	35.0	37.0	30.0	39.0
82-83	34.013072358856995	35.0	34.5	37.0	30.0	39.0
84-85	33.76501097756707	35.0	34.0	36.0	30.0	37.5
86-87	33.53588380868853	35.0	34.0	36.0	29.5	37.0
88-89	33.34568245996406	35.0	34.0	36.0	29.0	37.0
90-91	33.23192083061531	35.0	34.0	35.0	29.0	36.0
92-93	33.12510099434432	35.0	34.0	35.0	29.0	36.0
94-95	33.03038629836729	35.0	34.0	35.0	29.0	36.0
96-97	32.97197720564012	35.0	34.0	35.0	29.0	36.0
98-99	32.915165841621956	35.0	34.0	35.0	29.0	36.0
100	32.37894532451637	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.27853631420783387
1101	2	0.3342562658331616
1101	3	0.2955635919491115
1101	4	0.14922078032554964
1101	5	0.181421060318101
1101	6	0.14769305062313265
1101	7	0.1762016515816427
1101	8	0.13933188297929888
1101	9	0.26727790207667823
1101	10-11	0.23135913564780708
1101	12-13	0.24897140728722178
1101	14-15	0.3686106858667486
1101	16-17	0.4363648676128733
1101	18-19	0.31058541707683673
1101	20-21	0.3161801156345021
1101	22-23	0.33184560584636813
1101	24-25	0.3981503259836998
1101	26-27	0.38159176110611526
1101	28-29	0.3889015915699616
1101	30-31	0.37519408902887363
1101	32-33	0.4071813296180764
1101	34-35	0.38771703892274445
1101	36-37	0.3702710137021299
1101	38-39	0.40501833590212044
1101	40-41	0.40395299547652286
1101	42-43	0.37735865211375597
1101	44-45	0.44787062560769186
1101	46-47	0.41647233564904695
1101	48-49	0.3439765024631285
1101	50-51	0.38018730648842336
1101	52-53	0.3900604419061082
1101	54-55	0.37384184265859943
1101	56-57	0.40897069760456617
1101	58-59	0.3705922348482531
1101	60-61	0.3207936399777793
1101	62-63	0.22008267801575698
1101	64-65	0.1493091353463143
1101	66-67	0.13836680289090708
1101	68-69	0.20749819172458928
1101	70-71	0.1600368421958862
1101	72-73	0.18183112370017795
1101	74-75	0.1685348832945266
1101	76-77	0.21944891286381107
1101	78-79	0.16015228866741182
1101	80-81	0.20473208941922394
1101	82-83	0.18544727491176616
1101	84-85	0.17327552476353247
1101	86-87	0.1425746176546454
1101	88-89	0.14757058654454625
1101	90-91	0.13346112921709619
1101	92-93	0.12445224469976068
1101	94-95	0.12423958099779497
1101	96-97	0.09387958192630208
1101	98-99	0.11509774954851792
1101	100	0.16729267392644687
1102	1	0.10199510407091594
1102	2	0.05114467129704536
1102	3	0.1344176338920704
1102	4	0.0278933207565899
1102	5	0.08741041027735719
1102	6	0.10660206800805128
1102	7	0.10345141867448149
1102	8	0.12499241220499613
1102	9	0.08776251021058101
1102	10-11	0.0918674972487139
1102	12-13	0.17106093844486736
1102	14-15	0.22186450130366353
1102	16-17	0.2036402262769954
1102	18-19	0.1470368632295731
1102	20-21	0.24508482153620292
1102	22-23	0.2609728191093552
1102	24-25	0.2192002318050399
1102	26-27	0.2256627944851246
1102	28-29	0.2668640432915055
1102	30-31	0.2805752522006131
1102	32-33	0.17006764996224177
1102	34-35	0.3175382171733929
1102	36-37	0.2932204229450903
1102	38-39	0.24281772520003386
1102	40-41	0.2701860845139734
1102	42-43	0.2833489694963234
1102	44-45	0.29093698987932726
1102	46-47	0.23863348837906528
1102	48-49	0.2994826083553548
1102	50-51	0.3590097652850659
1102	52-53	0.3574021658093045
1102	54-55	0.35838693280793876
1102	56-57	0.34728601375366
1102	58-59	0.2989538937986467
1102	60-61	0.34643729089146547
1102	62-63	0.3260032764251193
1102	64-65	0.4109347418804177
1102	66-67	0.2583808240794099
1102	68-69	0.2657796416083542
1102	70-71	0.2052261524262846
1102	72-73	0.24947062049817248
1102	74-75	0.33349732630926354
1102	76-77	0.21488783443189874
1102	78-79	0.2855347030399642
1102	80-81	0.22448558351035786
1102	82-83	0.17178903138487556
1102	84-85	0.08464906915423853
1102	86-87	0.09948816322560106
1102	88-89	0.13117485752496805
1102	90-91	0.13629342219415008
1102	92-93	0.16559788673185238
1102	94-95	0.14579764203640622
1102	96-97	0.03202495404729433
1102	98-99	0.09279928720336983
1102	100	0.0037536330750640445
1103	1	0.22851547846219944
1103	2	0.28219306908680863
1103	3	0.21457697850681257
1103	4	0.18442566733824606
1103	5	0.19566688809904775
1103	6	0.15639467293288334
1103	7	0.05318341883229749
1103	8	0.07925101281052349
1103	9	0.0758291275914118
1103	10-11	0.2000790227618907
1103	12-13	0.22663169370185443
1103	14-15	0.243594576597161
1103	16-17	0.2247605348244761
1103	18-19	0.2566255787125016
1103	20-21	0.3070033925314206
1103	22-23	0.2613295269963132
1103	24-25	0.27285259070168166
1103	26-27	0.23857203027647955
1103	28-29	0.3163254887637095
1103	30-31	0.35895267437005884
1103	32-33	0.3147455230130447
1103	34-35	0.3339207464425229
1103	36-37	0.2497035160261234
1103	38-39	0.28399274620473136
1103	40-41	0.2915390778526259
1103	42-43	0.22851717844758923
1103	44-45	0.10715319560696202
1103	46-47	0.17577253589463027
1103	48-49	0.2101418355766569
1103	50-51	0.23836391380102384
1103	52-53	0.2182159125486507
1103	54-55	0.20880327526820963
1103	56-57	0.19916880400489845
1103	58-59	0.2000411898881822
1103	60-61	0.26023881383686387
1103	62-63	0.25908820782919406
1103	64-65	0.11954983563670396
1103	66-67	0.1723666838295017
1103	68-69	0.26574325934426923
1103	70-71	0.158171110749997
1103	72-73	0.0670989431690785
1103	74-75	0.0883303462700269
1103	76-77	0.053652473846469206
1103	78-79	0.012736444472984942
1103	80-81	0.05940227297657685
1103	82-83	0.028043029391454866
1103	84-85	0.0053856984296345445
1103	86-87	0.07732635173165647
1103	88-89	0.11299682309861225
1103	90-91	0.10596743064001402
1103	92-93	0.11523561640095892
1103	94-95	0.24248423648185025
1103	96-97	0.141709557008447
1103	98-99	0.17516226512888267
1103	100	0.26517750229812975
1104	1	0.13998377018319275
1104	2	0.155307408299592
1104	3	0.2357760000619038
1104	4	0.11309463484642635
1104	5	0.1230219279743281
1104	6	0.16564347475822672
1104	7	0.15888520331354528
1104	8	0.11082916631411877
1104	9	0.10791418331046998
1104	10-11	0.19425582901831007
1104	12-13	0.1413107782364591
1104	14-15	0.21176977189360002
1104	16-17	0.21936959885426432
1104	18-19	0.2623194196651326
1104	20-21	0.2266821092528275
1104	22-23	0.26852661119586685
1104	24-25	0.27942655608026
1104	26-27	0.2688200290945275
1104	28-29	0.270091370411933
1104	30-31	0.27017153311063424
1104	32-33	0.2602927888628557
1104	34-35	0.20435916740503046
1104	36-37	0.2294838357330562
1104	38-39	0.26996051835094903
1104	40-41	0.3506361490087144
1104	42-43	0.20061561143975837
1104	44-45	0.19558228709601622
1104	46-47	0.1760199598113914
1104	48-49	0.1576714290843526
1104	50-51	0.17372337238000313
1104	52-53	0.30988083287536483
1104	54-55	0.1919798398188064
1104	56-57	0.09942281146872034
1104	58-59	0.15507973609262393
1104	60-61	0.18450978645240212
1104	62-63	0.09703110591279085
1104	64-65	0.13008552000724194
1104	66-67	0.15638010066570018
1104	68-69	0.09967513910459047
1104	70-71	-0.03971795060927974
1104	72-73	-0.07705894698027294
1104	74-75	-0.0469226010263597
1104	76-77	0.09894372316880151
1104	78-79	0.033663624260697134
1104	80-81	-0.1097225603484091
1104	82-83	-0.08568743710076632
1104	84-85	-0.07050915397836377
1104	86-87	0.10077150645737021
1104	88-89	0.01873201985394246
1104	90-91	0.012458189874443804
1104	92-93	0.02665491787734453
1104	94-95	-0.018224227363347723
1104	96-97	0.07071326543087508
1104	98-99	0.03301359318177077
1104	100	0.023039586568764037
1105	1	0.19975748922931302
1105	2	0.13824184660642658
1105	3	0.11770839743711292
1105	4	0.090331275205223
1105	5	0.09197655516683767
1105	6	0.12424899493138497
1105	7	0.08538049258283564
1105	8	0.11513646999934224
1105	9	0.09808525919288513
1105	10-11	0.15447770135946826
1105	12-13	0.1516497265207022
1105	14-15	0.25786162300161664
1105	16-17	0.16810277675430285
1105	18-19	0.1269040373362742
1105	20-21	0.17056289079076237
1105	22-23	0.2605987621507708
1105	24-25	0.19012876959906322
1105	26-27	0.189115289251383
1105	28-29	0.23794342694348813
1105	30-31	0.2688724674637726
1105	32-33	0.22279439269055246
1105	34-35	0.29029830906706167
1105	36-37	0.2800224296026954
1105	38-39	0.24322439747925984
1105	40-41	0.15574707822126044
1105	42-43	0.11504983808308111
1105	44-45	0.11948640310932035
1105	46-47	0.14403580855144327
1105	48-49	0.18249756859523103
1105	50-51	0.1270924125075652
1105	52-53	0.14711284753065712
1105	54-55	0.09205532061225341
1105	56-57	0.014339647609993733
1105	58-59	0.09220537626689662
1105	60-61	0.1288390831033368
1105	62-63	0.006314018892510376
1105	64-65	0.11258201941845414
1105	66-67	-0.0057686193197596936
1105	68-69	0.061255081252681975
1105	70-71	0.10799172777073096
1105	72-73	0.11294084984503172
1105	74-75	-0.04322593473357017
1105	76-77	0.0624968256604177
1105	78-79	0.10439736604012495
1105	80-81	0.04456928257695125
1105	82-83	0.07169057388569655
1105	84-85	0.010872194639645727
1105	86-87	0.03222008363606932
1105	88-89	0.05033166994815019
1105	90-91	0.0694850147798789
1105	92-93	0.0509958038359386
1105	94-95	0.04692429751872851
1105	96-97	0.026464041878305977
1105	98-99	-6.894717225236491E-4
1105	100	-0.01904039913664235
1106	1	0.12508044926654804
1106	2	0.15649286234247484
1106	3	0.17944266453481106
1106	4	0.15191407289240288
1106	5	0.07100564148658606
1106	6	0.08103775956993786
1106	7	0.028883974806930723
1106	8	0.023433534532649958
1106	9	0.07871027753692772
1106	10-11	0.12383593752176836
1106	12-13	0.1021843506459561
1106	14-15	0.11696978264303937
1106	16-17	0.13301860902893026
1106	18-19	0.19345243731613948
1106	20-21	0.23827312404317524
1106	22-23	0.1999627793082226
1106	24-25	0.18077802304878787
1106	26-27	0.19151062860576218
1106	28-29	0.24070839882992345
1106	30-31	0.18292576268341776
1106	32-33	0.13424060585047215
1106	34-35	0.105242129115112
1106	36-37	0.221069250946357
1106	38-39	0.19433921758182038
1106	40-41	0.1816572573849342
1106	42-43	0.14192727260007842
1106	44-45	0.11277929568240097
1106	46-47	0.16137289769721264
1106	48-49	0.026806406861545895
1106	50-51	0.07823920366216441
1106	52-53	7.615569483832019E-4
1106	54-55	0.019486404651054556
1106	56-57	0.10771673935538928
1106	58-59	-0.017037743143411888
1106	60-61	0.08539055440066079
1106	62-63	0.07531711431929011
1106	64-65	0.12235415491700508
1106	66-67	0.09111650921435199
1106	68-69	0.03331041633027354
1106	70-71	0.023491320008794503
1106	72-73	2.7197979635218417E-4
1106	74-75	0.038001895176236644
1106	76-77	0.0734922469864756
1106	78-79	0.1210476016824984
1106	80-81	-0.008951699867274954
1106	82-83	0.046792330930855996
1106	84-85	-0.014028013748728085
1106	86-87	-0.08670449927273438
1106	88-89	-0.06804154334174939
1106	90-91	-0.039621441485820696
1106	92-93	-0.03083082815523852
1106	94-95	-0.07035102460271503
1106	96-97	-0.02943511513414876
1106	98-99	-0.010498164284690858
1106	100	-0.00250402760995172
1107	1	0.010783365796676492
1107	2	-0.02462283916962349
1107	3	0.037797259357148505
1107	4	0.04905235449594869
1107	5	0.06397569594720665
1107	6	0.016188267504958276
1107	7	0.08109751037798674
1107	8	0.08809381885142642
1107	9	0.07426096960814732
1107	10-11	0.05346284715458438
1107	12-13	0.0781183932157461
1107	14-15	0.11756617716264373
1107	16-17	0.14496064123715513
1107	18-19	0.07740128100323318
1107	20-21	0.11119086353726715
1107	22-23	0.14080122320130073
1107	24-25	0.11190835402580745
1107	26-27	0.08454241940830798
1107	28-29	0.1391945418007552
1107	30-31	0.12697439882716566
1107	32-33	0.038356166919705004
1107	34-35	0.09406375497532338
1107	36-37	0.05828573191634945
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2216	40-41	-0.5578265890023815
2216	42-43	-0.4635212218173521
2216	44-45	-0.46824142020387427
2216	46-47	-0.4599105686620746
2216	48-49	-0.40677990179741386
2216	50-51	-0.5326931807311723
2216	52-53	-0.48431600654567575
2216	54-55	-0.46911457076497953
2216	56-57	-0.380074618155092
2216	58-59	-0.4029669794711239
2216	60-61	-0.5070909365877938
2216	62-63	-0.5000526219535288
2216	64-65	-0.22458931551054206
2216	66-67	-0.32211997670491854
2216	68-69	-0.3234188968425258
2216	70-71	-0.31609072181043274
2216	72-73	-0.24483519973057355
2216	74-75	-0.217607110228748
2216	76-77	-0.2957928030919845
2216	78-79	-0.2672786041073536
2216	80-81	-0.3093605044418126
2216	82-83	-0.14748105516082433
2216	84-85	-0.3007885412030902
2216	86-87	-0.3157809617510239
2216	88-89	-0.18765088454040324
2216	90-91	-0.1505685572119404
2216	92-93	-0.259761995296536
2216	94-95	-0.23498315295339012
2216	96-97	-0.25477345147024977
2216	98-99	-0.24100376784152644
2216	100	-0.4200465022801332
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	9.0
8	36.0
9	80.0
10	173.0
11	172.0
12	144.0
13	121.0
14	172.0
15	314.0
16	560.0
17	1072.0
18	1809.0
19	3019.0
20	4541.0
21	6522.0
22	9521.0
23	11514.0
24	10938.0
25	9269.0
26	7957.0
27	8245.0
28	9077.0
29	10499.0
30	13225.0
31	16726.0
32	21442.0
33	28843.0
34	42122.0
35	67664.0
36	112074.0
37	268673.0
38	481508.0
39	226235.0
40	800.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.6347268546061	21.620573942058876	14.453280115666969	22.291419087668057
2	15.058261825221802	22.268535703108512	41.0808096246643	21.592392847005392
3	16.961160550982456	28.04255198458843	32.38378236469244	22.61250509973667
4	11.392816547727874	19.165690357703603	41.057482599156266	28.38401049541226
5	11.49317665614683	39.35997776720459	36.45210855075057	12.694737025898007
6	27.066204807053857	40.994210270243144	18.942983416253277	12.99660150644972
7	23.700490954324742	35.12596021895501	24.244678661631312	16.928870165088934
8	24.389761446086293	36.028164240984324	21.646060547882044	17.93601376504734
9	25.8307716586053	15.165696175559622	21.69922120724876	37.30431095858632
10-11	23.000384705729207	27.372285333839486	30.103041502403137	19.52428845802817
12-13	24.67225471737219	25.563622982567523	29.773314512569115	19.990807787491175
14-15	21.746317386705392	26.521833512535487	28.423945867738993	23.307903233020127
16-17	20.00757048514374	29.383299989746032	29.128805150547933	21.480324374562297
18-19	19.832307572594114	27.97861501574094	32.13190970396567	20.05716770769928
20-21	21.182631954428736	27.097028020976282	31.961191991430297	19.75914803316469
22-23	20.89977833887971	27.049819936701496	31.693431325777848	20.356970398640943
24-25	20.579756624538117	27.242074443831143	31.94821090018959	20.229958031441146
26-27	20.650407213559678	27.447190230074387	31.589140099063545	20.313262457302393
28-29	20.263090817129083	27.644316516208374	31.74601558531471	20.34657708134784
30-31	20.812101158118647	27.274875915859138	31.651000854498847	20.262022071523372
32-33	20.81236750529979	27.555152339360973	31.181552737890485	20.450927417448757
34-35	20.855744476604656	27.48869140255698	31.576622111035157	20.078942009803207
36-37	20.990466300067016	27.30272906205832	31.31885333795855	20.387951299916114
38-39	20.904973755833804	27.30796851790912	31.46947331676445	20.317584409492625
40-41	20.902450190228546	27.18408298131749	31.44052626201178	20.47294056644218
42-43	21.001841727769726	27.235790243024432	31.016186478118346	20.746181551087492
44-45	21.057538504389413	27.68021712341294	30.667954843042594	20.59428952915505
46-47	21.266964541544674	27.49404759307499	30.129178502393327	21.10980936298701
48-49	21.493939576850327	27.653266854245135	29.897445146389018	20.955348422515524
50-51	21.57234348688811	27.79697884141772	29.525329101786568	21.10534856990761
52-53	21.271334076816313	28.093147969233335	29.448798397451164	21.18671955649919
54-55	21.289756504816523	28.043798879756345	29.465993434458763	21.200451180968365
56-57	21.664188604696573	27.918722476331144	29.189860999449845	21.227227919522438
58-59	21.290162820303504	27.959588187573704	29.63560789167761	21.114641100445187
60-61	21.41339536257032	28.129294160469016	29.33961334510987	21.117697131850793
62-63	21.41111250094541	27.92648196738479	29.216930993757384	21.445474537912418
64-65	21.05967267862513	27.914061684664727	29.397033256861132	21.62923237984901
66-67	21.31722495571881	27.864623010800937	29.182677045670992	21.635474987809264
68-69	21.250780437801517	27.744204760697862	29.313516546372277	21.691498255128344
70-71	21.259482334186874	27.49638742248503	29.34063824263285	21.903492000695245
72-73	21.439293498695637	27.57568903586252	29.273833255393996	21.711184210047847
74-75	21.455383918334604	27.8999916728817	28.944081400672207	21.700543008111488
76-77	21.33684599035292	27.65897409324541	28.98091129086509	22.02326862553658
78-79	21.35315440648957	27.705565213660265	28.952339465270278	21.988940914579892
80-81	21.360476540586454	27.695430874052875	29.008448254170492	21.93564433119018
82-83	21.570584564903417	27.513101113103733	29.292568981742072	21.62374534025078
84-85	21.832881842808575	27.57009617657219	28.731487373415998	21.865534607203244
86-87	21.252425320491376	27.904166751510463	28.86596813557942	21.977439792418746
88-89	21.585157749634657	27.523817800417362	28.83749207770197	22.053532372246014
90-91	21.464252547311897	27.48031564777827	28.849075360870703	22.206356444039134
92-93	21.63140682303609	27.491515020613388	28.856893104895214	22.0201850514553
94-95	21.164232984771033	27.602308816160843	28.97594098366855	22.257517215399574
96-97	21.293256387031512	27.409883776952686	28.955148877712904	22.341710958302897
98-99	21.159642360155352	27.613420548255522	28.804595837086172	22.42234125450295
100	21.348912740725336	27.360491846555394	28.662468555986166	22.62812685673311
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	413.0
1	271.5
2	282.5
3	365.0
4	195.5
5	141.0
6	336.0
7	992.0
8	1946.0
9	2365.5
10	1622.0
11	806.0
12	755.5
13	849.5
14	986.5
15	1176.5
16	1388.0
17	1592.5
18	1879.0
19	2175.0
20	2495.5
21	3060.0
22	3764.5
23	4548.5
24	5630.5
25	6958.0
26	8855.0
27	11482.5
28	14477.0
29	18225.5
30	22564.5
31	26478.5
32	31146.0
33	35426.0
34	38679.0
35	42090.5
36	45643.5
37	49507.0
38	50521.0
39	49384.0
40	48977.0
41	49198.0
42	49173.0
43	49480.5
44	50618.5
45	53019.0
46	54462.5
47	53978.0
48	53590.0
49	51489.0
50	47852.5
51	45053.0
52	42237.5
53	38842.0
54	35998.0
55	31832.0
56	27221.5
57	22498.0
58	18149.0
59	15510.5
60	12423.0
61	9143.0
62	6723.0
63	4856.5
64	3310.5
65	2244.5
66	1594.0
67	1095.5
68	768.5
69	603.0
70	453.5
71	336.5
72	263.0
73	227.0
74	177.5
75	117.5
76	84.0
77	60.5
78	47.5
79	32.5
80	22.0
81	13.0
82	11.5
83	9.5
84	5.5
85	3.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006908704021665696
2	0.08050458265246237
3	4.363392013683597E-4
4	0.0
5	0.03861601932109984
6	0.004072499212771357
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	3.6361600114029975E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	3.636160011402998E-4
24-25	0.0
26-27	0.0
28-29	3.6361600114029975E-5
30-31	1.454464004561199E-4
32-33	0.0015999104050173191
34-35	0.0012362944038770193
36-37	5.454240017104497E-4
38-39	6.908704021665696E-4
40-41	0.001490825604675229
42-43	6.908704021665696E-4
44-45	4.7270080148238973E-4
46-47	2.1816960068417985E-4
48-49	5.817856018244796E-4
50-51	6.181472019385096E-4
52-53	0.001272656003991049
54-55	0.0013817408043331392
56-57	7.635936023946295E-4
58-59	0.0024362272076400085
60-61	0.0019271648060435887
62-63	9.454016029647795E-4
64-65	0.004908816015394047
66-67	0.004181584013113447
68-69	0.003454352010832848
70-71	0.0014544640045611992
72-73	0.005526963217332557
74-75	0.0037452448117450874
76-77	0.0035997984112889677
78-79	6.181472019385096E-4
80-81	0.0
82-83	2.1816960068417985E-4
84-85	1.454464004561199E-4
86-87	7.272320022805995E-5
88-89	1.818080005701499E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	3.999776012543298E-4
98-99	4.7270080148238973E-4
100	0.0010181248031928393
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1375077.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.357576979318374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.54430683869346	45.236871981051195
2	15.89428766320495	19.822585329794663
3	5.7301349925914815	10.719520017072275
4	2.525739795140879	6.2999605482090075
5	1.2873495107656185	4.0137998108427455
6	0.68284347884979	2.5548278878320807
7	0.4242783070435555	1.8519877034486372
8	0.25560337450908893	1.2751045681699245
9	0.17120855758538972	0.9608535728234101
>10	0.46546092940238715	4.222359855617652
>50	0.01115466145651282	0.47518780411210987
>100	0.0062581517729654495	0.7806762907890631
>500	9.158259892181973E-4	0.4016644503437501
>1k	1.5263766486969953E-4	0.2998206205172542
>5k	3.0527532973939907E-4	1.084779559376237
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5970	0.4341575053615179	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5427	0.3946688076376814	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3150	0.2290780807183889	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	7.272320022805995E-5	0.0
6	7.272320022805995E-5	0.0	0.0	7.272320022805995E-5	0.0
7	7.272320022805995E-5	0.0	0.0	7.272320022805995E-5	0.0
8	7.272320022805995E-5	0.0	0.0	7.272320022805995E-5	0.0
9	7.272320022805995E-5	0.0	0.0	7.272320022805995E-5	0.0
10-11	7.272320022805995E-5	0.0	0.0	7.272320022805995E-5	0.0
12-13	7.272320022805995E-5	0.0	0.0	7.272320022805995E-5	1.0908480034208993E-4
14-15	7.272320022805995E-5	0.0	0.0	7.272320022805995E-5	1.454464004561199E-4
16-17	1.454464004561199E-4	0.0	0.0	7.272320022805995E-5	1.454464004561199E-4
18-19	1.454464004561199E-4	0.0	0.0	1.0908480034208993E-4	1.454464004561199E-4
20-21	1.8180800057014988E-4	0.0	0.0	2.545312007982099E-4	1.454464004561199E-4
22-23	2.1816960068417988E-4	0.0	0.0	6.181472019385096E-4	2.1816960068417988E-4
24-25	2.1816960068417988E-4	0.0	0.0	0.001454464004561199	2.1816960068417988E-4
26-27	2.1816960068417988E-4	0.0	0.0	0.0025816736080961286	2.1816960068417988E-4
28-29	2.1816960068417988E-4	0.0	0.0	0.007017788822007785	2.1816960068417988E-4
30-31	2.1816960068417988E-4	0.0	0.0	0.015744572849374982	2.1816960068417988E-4
32-33	2.1816960068417988E-4	0.0	0.0	0.03596162251277565	2.1816960068417988E-4
34-35	2.1816960068417988E-4	0.0	0.0	0.07006880341973577	2.1816960068417988E-4
36-37	2.1816960068417988E-4	0.0	0.0	0.12195680678245654	2.1816960068417988E-4
38-39	2.1816960068417988E-4	0.0	0.0	0.19602538621473561	2.908928009122398E-4
40-41	2.1816960068417988E-4	0.0	0.0	0.2711120904502075	2.908928009122398E-4
42-43	2.545312007982098E-4	0.0	0.0	0.34347167467712714	3.2725440102626983E-4
44-45	2.908928009122398E-4	0.0	0.0	0.4332121037585531	3.636160011402998E-4
46-47	2.908928009122398E-4	0.0	0.0	0.5354609232792055	3.636160011402998E-4
48-49	2.908928009122398E-4	0.0	0.0	0.6484364148334967	3.636160011402998E-4
50-51	2.908928009122398E-4	0.0	0.0	0.7662116376028396	4.3633920136835976E-4
52-53	2.908928009122398E-4	0.0	0.0	0.8748237371434473	4.3633920136835976E-4
54-55	2.908928009122398E-4	0.0	0.0	0.9883082910993348	4.7270080148238973E-4
56-57	2.908928009122398E-4	0.0	0.0	1.1208463235149742	5.090624015964197E-4
58-59	2.908928009122398E-4	0.0	0.0	1.267383571974515	5.090624015964197E-4
60-61	2.908928009122398E-4	0.0	0.0	1.4199204844528706	5.090624015964197E-4
62-63	2.908928009122398E-4	0.0	0.0	1.5703120625244986	5.817856018244796E-4
64-65	2.908928009122398E-4	0.0	0.0	1.7279032374187047	5.817856018244796E-4
66-67	2.908928009122398E-4	0.0	0.0	1.8964028923471195	6.181472019385096E-4
68-69	2.908928009122398E-4	0.0	0.0	2.0880285249480575	6.545088020525396E-4
70-71	2.908928009122398E-4	0.0	0.0	2.292998864790844	6.545088020525396E-4
72-73	2.908928009122398E-4	0.0	0.0	2.499423668638193	7.635936023946295E-4
74-75	2.908928009122398E-4	0.0	0.0	2.7016305268723135	8.726784027367195E-4
76-77	2.908928009122398E-4	0.0	0.0	2.919581957955809	8.726784027367195E-4
78-79	2.908928009122398E-4	0.0	0.0	3.1569141219000825	8.726784027367195E-4
80-81	2.908928009122398E-4	0.0	0.0	3.4141724427068447	8.726784027367195E-4
82-83	2.908928009122398E-4	0.0	0.0	3.678593998736071	9.454016029647795E-4
84-85	3.636160011402998E-4	0.0	0.0	3.937306783547394	9.454016029647795E-4
86-87	3.636160011402998E-4	0.0	0.0	4.208709766798513	9.817632030788095E-4
88	3.636160011402998E-4	0.0	0.0	4.418079860255099	0.0010181248031928393
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3705	0.0	57.917587	1
GTATCAA	6390	0.0	38.589066	1
ATCAACG	8035	0.0	30.076807	3
CAACGCA	8115	0.0	29.780302	5
TATCAAC	8385	0.0	29.347372	2
TCAACGC	8340	0.0	29.033249	4
AACGCAG	8540	0.0	28.507072	6
ACGCAGA	9235	0.0	26.157196	7
CGCAGAG	9315	0.0	25.932547	8
TACATGG	4825	0.0	23.98879	2
GCAGAGT	10065	0.0	23.626623	9
CATCGTG	100	0.0017596299	23.498219	9
ACATGGG	4725	0.0	23.483599	3
GAGTACT	7135	0.0	23.448816	12-13
GTACATG	5050	0.0	23.389242	1
AGAGTAC	9305	0.0	22.323938	10-11
ACTGCGC	360	0.0	22.192762	8
CAGAGTA	9880	0.0	22.190119	10-11
GTACTTT	7445	0.0	22.156815	14-15
CTGCGCA	375	0.0	21.30505	9
>>END_MODULE