FastQCFastQC Report
Mon 16 Jan 2017
ERR1146497_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146497_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659882
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT70730.42611462742532297No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC19430.11705651365578999No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT18790.11320081788946443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG55650.026.2713621
TACATGG54300.025.7897282
ACATGGG54500.023.9705853
CATGGGG41300.023.2118874
CTAACGC1703.4580244E-622.114213
TCTAACG1805.6748413E-620.8856432
TTACGTA1354.0823864E-420.8856414
TAGTACT7450.020.184784
ATGGGGG23600.020.1112795
GTACTAG6550.020.0957451
GAGTACA41950.017.3694931
GTATATA8600.016.9453541
CTAGTAC7600.016.6947733
GTATAAT6900.016.3511961
AGTACAT41150.016.3303892
AGAGTAC40050.016.30859210-11
GTATTAA11700.016.071691
ACGTGCG2951.3778736E-615.9287679
CCTAGTA8400.015.6642312
TTAGTAC4201.0040822E-915.6642313
Produced by FastQC (version 0.11.5)