##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146497_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1659882
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.582566110121082	31.0	31.0	34.0	27.0	34.0
2	30.795896937252166	31.0	31.0	34.0	27.0	34.0
3	31.065560684434196	31.0	31.0	34.0	27.0	34.0
4	34.68301240690603	37.0	35.0	37.0	32.0	37.0
5	34.88069814601279	37.0	35.0	37.0	32.0	37.0
6	34.910181567123445	37.0	35.0	37.0	32.0	37.0
7	34.79569571812936	37.0	35.0	37.0	31.0	37.0
8	34.79209184749277	37.0	35.0	37.0	32.0	37.0
9	36.388922224591866	39.0	37.0	39.0	32.0	39.0
10-11	36.379656806929646	39.0	37.0	39.0	32.0	39.0
12-13	36.34824674284076	39.0	37.0	39.0	32.0	39.0
14-15	37.67104137523029	40.0	38.0	41.0	32.0	41.0
16-17	37.6868220150589	40.0	38.0	41.0	32.0	41.0
18-19	37.67636976604361	40.0	38.0	41.0	31.0	41.0
20-21	37.77268986590613	40.0	38.0	41.0	31.5	41.0
22-23	37.78850092958415	40.0	38.0	41.0	31.5	41.0
24-25	37.83379601682529	40.0	38.0	41.0	32.0	41.0
26-27	37.855239709810704	40.0	38.0	41.0	32.0	41.0
28-29	37.82583008912682	40.0	38.0	41.0	31.0	41.0
30-31	37.812902965391515	40.0	38.0	41.0	31.0	41.0
32-33	37.81851902725616	40.0	38.0	41.0	31.0	41.0
34-35	37.75908709173302	40.0	38.0	41.0	31.0	41.0
36-37	37.65522729928995	40.0	38.0	41.0	31.0	41.0
38-39	37.60128973023383	40.0	38.0	41.0	30.5	41.0
40-41	37.551882302476926	40.0	37.5	41.0	30.0	41.0
42-43	37.426866186873525	40.0	37.0	41.0	30.0	41.0
44-45	37.27795078204354	40.0	37.0	41.0	30.0	41.0
46-47	37.161479249729794	40.0	37.0	41.0	30.0	41.0
48-49	37.04204184393831	40.0	36.0	41.0	30.0	41.0
50-51	36.91694439725234	40.0	36.0	41.0	30.0	41.0
52-53	36.81194235493848	40.0	35.5	41.0	30.0	41.0
54-55	36.63096232141803	39.0	35.0	41.0	29.0	41.0
56-57	36.486091179975446	39.0	35.0	41.0	29.0	41.0
58-59	36.2671421823961	39.0	35.0	41.0	29.0	41.0
60-61	36.06708519039305	38.5	35.0	40.0	29.0	41.0
62-63	35.86063075568022	38.0	35.0	40.0	28.0	41.0
64-65	35.616338390319314	37.5	34.5	40.0	28.0	41.0
66-67	35.34017387983002	37.0	34.0	40.0	28.0	41.0
68-69	35.02612053145947	36.5	34.0	39.0	28.0	41.0
70-71	34.72696432637983	36.0	34.0	39.0	28.0	41.0
72-73	34.41581871482431	35.0	34.0	39.0	27.5	40.5
74-75	34.11868012304489	35.0	34.0	37.5	27.0	40.0
76-77	33.82022457018029	35.0	33.0	37.0	27.0	39.0
78-79	33.535650726979384	35.0	33.0	37.0	27.0	39.0
80-81	33.23825247818821	35.0	33.0	36.0	26.0	38.5
82-83	32.99548612491731	35.0	33.0	36.0	26.0	37.0
84-85	32.79428356955495	35.0	33.0	35.5	26.0	37.0
86-87	32.63294559492783	35.0	33.0	35.0	26.0	36.5
88-89	32.48295511367675	35.0	33.0	35.0	26.0	36.0
90-91	32.32226085950688	35.0	33.0	35.0	25.5	36.0
92-93	32.21728954226867	35.0	33.0	35.0	25.0	36.0
94-95	32.12844135908456	35.0	33.0	35.0	25.0	35.5
96-97	32.07252804717444	35.0	32.0	35.0	25.0	35.0
98-99	32.01400581487118	35.0	32.0	35.0	25.0	35.0
100	31.048093780160276	34.0	30.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1844115576390557
1101	2	0.3157192269147906
1101	3	0.360269419173477
1101	4	0.36476981681957454
1101	5	0.3824784242083439
1101	6	0.3870940993576326
1101	7	0.25718207383149405
1101	8	0.3117393859841471
1101	9	0.4306156136773822
1101	10-11	0.43429484260607865
1101	12-13	0.4757892109215618
1101	14-15	0.5918832713184088
1101	16-17	0.6259231291495055
1101	18-19	0.4386761050237027
1101	20-21	0.4688320311165626
1101	22-23	0.4058551555126968
1101	24-25	0.45569683363248714
1101	26-27	0.45237104499027936
1101	28-29	0.4918144375743623
1101	30-31	0.540085623480266
1101	32-33	0.5380182650461478
1101	34-35	0.5277990179514234
1101	36-37	0.43590743244809715
1101	38-39	0.4440168845910577
1101	40-41	0.4892614170661176
1101	42-43	0.45811304589318524
1101	44-45	0.477991323700671
1101	46-47	0.44152081810890564
1101	48-49	0.4746320803594344
1101	50-51	0.3501468708887714
1101	52-53	0.41439880392599804
1101	54-55	0.5585190293349243
1101	56-57	0.445392108263178
1101	58-59	0.41268410943004596
1101	60-61	0.4036635383518785
1101	62-63	0.3973068053498423
1101	64-65	0.550263108346293
1101	66-67	0.471313155366488
1101	68-69	0.4505466509977083
1101	70-71	0.32836706585616326
1101	72-73	0.3985581356599752
1101	74-75	0.302770378923924
1101	76-77	0.3772719617328235
1101	78-79	0.3934049889857505
1101	80-81	0.40714444354407675
1101	82-83	0.41580175137661257
1101	84-85	0.4539570642995656
1101	86-87	0.4354287882152903
1101	88-89	0.39429085795492824
1101	90-91	0.4348799420129197
1101	92-93	0.38679804614204727
1101	94-95	0.4250449953284914
1101	96-97	0.33914573936260695
1101	98-99	0.3739866016722715
1101	100	0.4511586511254144
1102	1	0.12489282477277186
1102	2	0.18559741790172524
1102	3	0.16146074147354028
1102	4	0.19320648320933032
1102	5	0.2202561404508785
1102	6	0.24290533384690605
1102	7	0.1317197134011181
1102	8	0.19973601385284923
1102	9	0.44281312949962626
1102	10-11	0.38118838971284674
1102	12-13	0.37607719585773225
1102	14-15	0.45339482302185985
1102	16-17	0.41696544385919765
1102	18-19	0.32094410946653795
1102	20-21	0.36683244340269283
1102	22-23	0.3521271966674675
1102	24-25	0.45848868759705397
1102	26-27	0.36151733579968237
1102	28-29	0.4440960105382388
1102	30-31	0.3669626791419631
1102	32-33	0.4055658087130922
1102	34-35	0.37069185124937576
1102	36-37	0.4057317800976108
1102	38-39	0.39227128359171815
1102	40-41	0.3088945439487105
1102	42-43	0.34826617634482204
1102	44-45	0.3499423293403794
1102	46-47	0.31296969616759185
1102	48-49	0.3408849225184625
1102	50-51	0.2905733716564285
1102	52-53	0.2671837351757489
1102	54-55	0.19504604176811569
1102	56-57	0.15902462594686995
1102	58-59	0.12428657945469013
1102	60-61	0.07760428606661662
1102	62-63	0.22040744316263527
1102	64-65	0.1431730176741084
1102	66-67	0.1714703779117741
1102	68-69	0.14391099809340346
1102	70-71	0.1954210968760819
1102	72-73	0.13554389332803396
1102	74-75	0.09961823611293852
1102	76-77	0.15505896979701816
1102	78-79	0.10388505618223576
1102	80-81	0.03380058768423311
1102	82-83	0.1299450115753089
1102	84-85	0.12309867843052302
1102	86-87	0.06684652944113623
1102	88-89	0.07008904697273266
1102	90-91	0.1584879379023576
1102	92-93	0.1553547677904561
1102	94-95	0.1480428692858453
1102	96-97	0.17946763021755174
1102	98-99	0.09521041458052082
1102	100	0.04187175228627993
1103	1	0.297911206501702
1103	2	0.15485933724928103
1103	3	0.355290000142535
1103	4	0.2842071531187358
1103	5	0.33927798053951363
1103	6	0.2995390300695391
1103	7	0.3473712258473469
1103	8	0.3996694036565529
1103	9	0.4086375298725997
1103	10-11	0.486520872583867
1103	12-13	0.5076833332138051
1103	14-15	0.5401705348281496
1103	16-17	0.46913070608073326
1103	18-19	0.4628318432309584
1103	20-21	0.44039482844048194
1103	22-23	0.3876000318711519
1103	24-25	0.39263266541610164
1103	26-27	0.46620706233917275
1103	28-29	0.41233703444355996
1103	30-31	0.38403104096197893
1103	32-33	0.3364731602223543
1103	34-35	0.3857280409858319
1103	36-37	0.4008051505074164
1103	38-39	0.35538187956260714
1103	40-41	0.4088345867563845
1103	42-43	0.28108496394706606
1103	44-45	0.3904821685100188
1103	46-47	0.2338129764432182
1103	48-49	0.3278163787427957
1103	50-51	0.33668620379813063
1103	52-53	0.2857168600215729
1103	54-55	0.32175811318290215
1103	56-57	0.2634956249615499
1103	58-59	0.2735879038233051
1103	60-61	0.23827553408799673
1103	62-63	0.10041761103676095
1103	64-65	0.08888650926477482
1103	66-67	0.07989949346102776
1103	68-69	-0.00991076191871798
1103	70-71	0.13946853199275466
1103	72-73	0.18099252722785053
1103	74-75	0.1810890790224846
1103	76-77	0.03815867648370386
1103	78-79	0.06696238713330871
1103	80-81	0.2018505581565151
1103	82-83	0.08863286284940841
1103	84-85	0.0661672001630933
1103	86-87	0.15218853893289008
1103	88-89	0.11980181555019698
1103	90-91	0.1943437216172086
1103	92-93	0.12505704786708804
1103	94-95	0.15104644006530066
1103	96-97	0.17960419715127784
1103	98-99	0.17523783617878053
1103	100	0.0415785792502632
1104	1	-0.14223195570562552
1104	2	-0.09780120512772328
1104	3	-0.06781620991107218
1104	4	-0.08350199401090919
1104	5	0.10904284947297072
1104	6	0.07760028217335702
1104	7	0.04227473487794242
1104	8	0.10996544416929055
1104	9	0.11734066033743318
1104	10-11	0.17374976526595987
1104	12-13	0.20670806540522335
1104	14-15	0.3215269737867601
1104	16-17	0.3093744259838829
1104	18-19	0.20039193429278157
1104	20-21	0.19383456231718554
1104	22-23	0.2529883081278541
1104	24-25	0.24909765928147465
1104	26-27	0.24138353288349634
1104	28-29	0.32122192648961345
1104	30-31	0.27910231412170106
1104	32-33	0.2750617654005154
1104	34-35	0.1899720871229249
1104	36-37	0.24899335763536357
1104	38-39	0.268349268485359
1104	40-41	0.2770874882415697
1104	42-43	0.32024647550765906
1104	44-45	0.22840137820267614
1104	46-47	0.16668343580973755
1104	48-49	0.2338760250589118
1104	50-51	0.2274163071665143
1104	52-53	0.17722979422720186
1104	54-55	0.21623785401727957
1104	56-57	0.2864278373540259
1104	58-59	0.22595056175655515
1104	60-61	0.18413432256559759
1104	62-63	0.23755260881023332
1104	64-65	0.12763770884641445
1104	66-67	0.1421252600454892
1104	68-69	0.1355949921538837
1104	70-71	0.11733380110403857
1104	72-73	0.10789969873844285
1104	74-75	4.587718885247227E-4
1104	76-77	0.07589002170604431
1104	78-79	0.14675878206153214
1104	80-81	0.2072152216447023
1104	82-83	0.19771388719414773
1104	84-85	0.23742985246003911
1104	86-87	0.17373416372129213
1104	88-89	0.14699905159464066
1104	90-91	0.14711949101982924
1104	92-93	0.16804882221771322
1104	94-95	0.06690560927359002
1104	96-97	0.13157324673174742
1104	98-99	0.1225662638095244
1104	100	0.06420742019777492
1105	1	-0.0722964864033031
1105	2	0.028031918314376014
1105	3	0.12476996853411393
1105	4	0.13055423367909924
1105	5	0.08872149875774937
1105	6	0.08686734198801815
1105	7	0.037979231182411866
1105	8	0.11609125731687442
1105	9	0.3236132525954929
1105	10-11	0.20054633289291246
1105	12-13	0.18713502759874245
1105	14-15	0.27033337300856886
1105	16-17	0.1541569931418394
1105	18-19	0.11082749478922693
1105	20-21	0.18191523003186916
1105	22-23	0.21105421107994005
1105	24-25	0.3047699336628824
1105	26-27	0.3043943285304991
1105	28-29	0.264790569607932
1105	30-31	0.17373080663944052
1105	32-33	0.21688260803420434
1105	34-35	0.33775997828057314
1105	36-37	0.3506125309823602
1105	38-39	0.2757629163370936
1105	40-41	0.23640544306140043
1105	42-43	0.20390587849013286
1105	44-45	0.1003561459182194
1105	46-47	0.17733959922960452
1105	48-49	0.26375286956858446
1105	50-51	0.17820587455897652
1105	52-53	0.2481201962630024
1105	54-55	0.20398084292633456
1105	56-57	0.15199892968590945
1105	58-59	0.178228677772843
1105	60-61	0.21295965542413597
1105	62-63	0.18985747510227924
1105	64-65	0.1657433898059466
1105	66-67	0.13663797945420697
1105	68-69	0.11922608153281544
1105	70-71	0.07609364789791329
1105	72-73	0.11475141709625802
1105	74-75	0.13457345066809268
1105	76-77	0.03618305881770567
1105	78-79	0.1501551117152573
1105	80-81	0.2626827000838077
1105	82-83	0.23302268786117253
1105	84-85	0.1465250330678316
1105	86-87	0.1919385948871053
1105	88-89	0.06368983404021122
1105	90-91	0.1972442966894974
1105	92-93	0.20022488766903734
1105	94-95	0.19780278572986987
1105	96-97	0.18247042318802897
1105	98-99	0.1329341669526798
1105	100	0.035259452958175785
1106	1	-0.1324188056301736
1106	2	-0.020265926032074333
1106	3	-0.00481778765930585
1106	4	0.022575302126085717
1106	5	0.0027023062683753096
1106	6	-0.022231052412969632
1106	7	-0.1523822514182953
1106	8	0.03266445149546371
1106	9	0.008287527784041515
1106	10-11	0.12877032732446025
1106	12-13	-0.008482192164173341
1106	14-15	0.08945820907449331
1106	16-17	0.008644478976663095
1106	18-19	0.054126973171150894
1106	20-21	-0.003085342941609781
1106	22-23	-0.02405590593397733
1106	24-25	-0.010839137143904054
1106	26-27	0.04599177858630554
1106	28-29	0.06164982610057024
1106	30-31	0.15580115080550172
1106	32-33	0.12578186231983324
1106	34-35	0.11792363648279292
1106	36-37	0.15026764339126686
1106	38-39	0.12792429962239282
1106	40-41	0.051480502586969124
1106	42-43	-0.07792373193034052
1106	44-45	-0.038617510034548275
1106	46-47	-0.04597354563082234
1106	48-49	0.037198816948396995
1106	50-51	0.1428180988420067
1106	52-53	0.08536601886192585
1106	54-55	0.07889190470985596
1106	56-57	-0.06232744449548022
1106	58-59	-0.01869466686433441
1106	60-61	-0.01930953175993011
1106	62-63	0.01004965465094898
1106	64-65	0.0016102562352173777
1106	66-67	0.05019792733483541
1106	68-69	0.011298675150314352
1106	70-71	0.07350550547793233
1106	72-73	0.07081793295064642
1106	74-75	0.16437715419888121
1106	76-77	0.1018434425826058
1106	78-79	-4.3335326757443227E-4
1106	80-81	-0.02058791741183086
1106	82-83	0.0372816209421174
1106	84-85	0.11738181940813774
1106	86-87	0.1181155036315289
1106	88-89	0.027165448713425633
1106	90-91	0.073688126189559
1106	92-93	-0.031036715043889274
1106	94-95	0.13218229789600855
1106	96-97	0.002942977758451093
1106	98-99	0.008760203716363435
1106	100	-0.03320162516569525
1107	1	0.009316252190142649
1107	2	0.017779800319072336
1107	3	-0.028979138335337495
1107	4	0.0014371570229840813
1107	5	0.1748539882953608
1107	6	0.12369902515633413
1107	7	0.12007783279514683
1107	8	0.11486639256236231
1107	9	0.14731438559538645
1107	10-11	0.2937748722416984
1107	12-13	0.20045951468662793
1107	14-15	0.304335618593953
1107	16-17	0.2536629643575168
1107	18-19	0.08641594023170995
1107	20-21	0.14858461862021244
1107	22-23	0.22291090190366702
1107	24-25	0.26977554762633815
1107	26-27	0.2764755778925476
1107	28-29	0.3064849658516806
1107	30-31	0.3064918559402443
1107	32-33	0.2477369512004728
1107	34-35	0.22308201563894414
1107	36-37	0.25261518485599055
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2216	64-65	-0.5283773333376303
2216	66-67	-0.626909756986862
2216	68-69	-0.6372639080084994
2216	70-71	-0.5930196047541116
2216	72-73	-0.5855959607612036
2216	74-75	-0.5343377251872781
2216	76-77	-0.5123822332785153
2216	78-79	-0.5390743070805044
2216	80-81	-0.6334722379111142
2216	82-83	-0.5591822277222747
2216	84-85	-0.5501479835166663
2216	86-87	-0.5126770483222174
2216	88-89	-0.5445475034766005
2216	90-91	-0.5001750220016774
2216	92-93	-0.5388181423623415
2216	94-95	-0.5832094658762834
2216	96-97	-0.49173023421522544
2216	98-99	-0.5209803706238887
2216	100	-0.5565708174736166
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	58.0
1	28.0
2	21.0
3	20.0
4	18.0
5	40.0
6	42.0
7	254.0
8	624.0
9	883.0
10	1300.0
11	2383.0
12	3680.0
13	4683.0
14	5231.0
15	5330.0
16	5623.0
17	6104.0
18	6561.0
19	7259.0
20	7935.0
21	8708.0
22	9515.0
23	10762.0
24	12570.0
25	15536.0
26	17566.0
27	16733.0
28	17632.0
29	21705.0
30	24571.0
31	27853.0
32	35498.0
33	48126.0
34	69724.0
35	107900.0
36	200920.0
37	396802.0
38	443884.0
39	115631.0
40	169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.58325777889234	19.383933910762167	13.332324601169004	23.70048370917649
2	15.18021866523348	20.976787455127596	39.444636580213604	24.398357299425317
3	18.899287870909355	25.75233284187047	30.112413845876684	25.235965441343495
4	12.408274472542443	17.1187444530872	39.04779578564168	31.425185288728674
5	13.129218868718054	38.66847185894435	35.03663708826583	13.16567218407177
6	30.267891734647336	38.19673030006694	17.87856932401337	13.656808641272356
7	26.88021554105468	32.97086496572225	22.52119653722114	17.627722956001936
8	25.678122290658866	34.69282134179075	20.48486323682782	19.144193130722563
9	25.92693902708682	16.384583822617678	20.484389996728396	37.2040871535671
10-11	24.563269149837037	26.31245193166547	28.645820310511272	20.47845860798622
12-13	25.526602687787708	24.321101793535416	27.885759834289942	22.26653568438693
14-15	22.594518774212073	25.83703459123381	25.797177594402786	25.771269040151328
16-17	22.38099943483694	28.584417165050507	26.32189711622235	22.7126862838902
18-19	22.34871227277506	28.621071001758423	27.138115828119876	21.89210089734664
20-21	23.512726028288633	27.301102336017273	26.55936322863471	22.626808407059386
22-23	23.218899996445423	27.216144294208267	26.528724912355532	23.03623079699078
24-25	23.291060384375665	27.386080984643712	26.565353774245644	22.75750485673498
26-27	22.92197923523112	27.18904434767808	26.949525013066474	22.93945140402432
28-29	22.67389054825285	28.086349183397125	26.588372804104175	22.651387464245854
30-31	23.052359052438582	27.300292279448314	26.441167840463986	23.206180827649117
32-33	23.009480072206166	27.521220804824026	26.41803418947612	23.051264933493684
34-35	23.59138062864797	27.369420307931097	26.483936138611774	22.555262924809163
36-37	23.35408577192564	27.03309423438927	26.804600485428704	22.808219508256386
38-39	22.978710455323046	27.49756409429802	26.743924697522882	22.77980075285605
40-41	23.037229704344284	27.415077052583882	26.627061517033095	22.92063172603874
42-43	22.822281384493568	27.39956708965414	26.30749891832674	23.47065260752555
44-45	23.034711010752766	27.19784474628281	26.525729079473326	23.241715163491097
46-47	23.40744994928789	27.451492044858067	26.340917952448134	22.800140053405908
48-49	23.140090198811635	27.35253199400121	26.462175046682873	23.045202760504285
50-51	22.58235009805233	27.48370113504464	26.894061295062187	23.039887471840842
52-53	23.00513394726962	27.605015707986336	26.3155626882141	23.07428765652995
54-55	22.639509777775768	27.193441605306017	26.672331885387802	23.494716731530406
56-57	23.134093799995842	27.080422167182327	26.454247090692174	23.331236942129657
58-59	23.479490081639494	27.018868811496304	26.47814127621824	23.023499830645967
60-61	22.97054389336268	27.307883197733407	26.917491621874344	22.804081287029568
62-63	22.77513896576984	27.29948535883708	26.704389139712465	23.220986535680613
64-65	22.49557185052954	27.673870030875	26.49877566714539	23.331782451450064
66-67	22.91954763531299	27.121359733829387	26.243666891368413	23.71542573948922
68-69	22.759228381826414	27.16816082078679	26.559218557746757	23.51339223964004
70-71	23.103815091610414	26.924993972999033	26.412789296046284	23.55840163934426
72-73	22.937239777240126	26.79681583920211	26.962550824139814	23.30339355941795
74-75	22.621444389900958	27.732952674032145	26.49680438445263	23.14879855161426
76-77	22.89616450566846	26.97430216688449	26.43938391737591	23.690149410071147
78-79	22.922334415353518	27.06497906875549	26.59874207620106	23.413944439689928
80-81	23.137514423710062	27.020592643086673	26.612434420901415	23.229458512301854
82-83	23.033487441983247	26.82563302372984	27.059387579337024	23.08149195494989
84-85	22.90825188244072	27.282643938763872	26.65411907077881	23.1549851080166
86-87	22.731528313013218	27.49984707558805	26.564311269127984	23.204313342270748
88-89	22.7990626481185	27.171995392186904	26.243321578769592	23.785620380925003
90-91	22.96037868490223	26.805956531027892	26.49998809754469	23.733676686525186
92-93	23.261043881421955	27.03452132960199	26.668187407960747	23.036247381015304
94-95	22.849701336107323	26.88559645086887	26.863959817336188	23.400742395687622
96-97	22.48084813899326	26.867146365930044	27.249385058248837	23.402620436827863
98-99	22.73155974194637	27.239091328204807	26.46773274997062	23.561616179878207
100	22.818292045019007	26.38641475049838	26.559370081017818	24.235923123464794
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	713.0
1	596.0
2	493.0
3	507.0
4	494.0
5	431.5
6	356.5
7	330.0
8	336.5
9	339.0
10	308.5
11	271.0
12	273.0
13	285.0
14	309.0
15	356.5
16	399.0
17	448.5
18	554.5
19	693.5
20	874.0
21	1208.5
22	1683.5
23	2391.0
24	3428.5
25	4920.0
26	6730.0
27	8920.0
28	11314.0
29	14101.0
30	17712.0
31	21304.5
32	25338.0
33	28494.5
34	30677.5
35	33082.5
36	36549.0
37	40952.5
38	43817.5
39	45559.0
40	47166.5
41	48921.0
42	50268.5
43	52213.5
44	55052.0
45	58908.5
46	62447.5
47	68490.0
48	79284.0
49	77946.0
50	67015.0
51	62988.5
52	62152.0
53	61169.5
54	59853.0
55	56289.0
56	51141.0
57	45551.0
58	40342.5
59	35256.0
60	29356.0
61	24147.0
62	19849.0
63	15295.5
64	11035.0
65	7910.0
66	5770.0
67	4305.0
68	3230.0
69	2333.5
70	1773.0
71	1417.5
72	1047.0
73	776.5
74	557.5
75	411.0
76	298.0
77	207.0
78	132.5
79	82.5
80	65.0
81	52.5
82	38.5
83	29.5
84	25.0
85	16.5
86	10.5
87	9.0
88	8.0
89	8.5
90	6.0
91	4.0
92	4.0
93	1.5
94	2.5
95	4.0
96	2.0
97	2.0
98	3.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0622935847246973
2	0.010603163357395286
3	0.012711746979604572
4	0.010542918111046448
5	0.01355518042848829
6	0.01319370895039527
7	0.003554469534581374
8	0.006928203330116237
9	0.008735560720581343
10-11	0.008163230880267392
12-13	0.006958325953290656
14-15	0.011958681400244114
16-17	0.01108512532818598
18-19	0.00936813580724413
20-21	0.008072863010744136
22-23	0.0030725075637906794
24-25	0.004428025606639508
26-27	0.005994402011709266
28-29	0.006596854475197635
30-31	0.010181446632953428
32-33	0.011446596806279
34-35	0.01355518042848829
36-37	0.019157988338930117
38-39	0.020182157526860343
40-41	0.02009178965733709
42-43	0.02506202248111613
44-45	0.029821396942674237
46-47	0.031026301869650975
48-49	0.03298427237598817
50-51	0.033978318940743976
52-53	0.032231206796627714
54-55	0.03822560880833698
56-57	0.041057135386732314
58-59	0.038315976677860235
60-61	0.03918953274991836
62-63	0.034369913042011424
64-65	0.03680984551913931
66-67	0.03888830651817418
68-69	0.04178007834291835
70-71	0.04108725800990672
72-73	0.03653874191056955
74-75	0.03949075898166255
76-77	0.03955100422801139
78-79	0.038556957663255585
80-81	0.043466945240685784
82-83	0.03994259832927883
84-85	0.0361471478093021
86-87	0.034068686810267236
88-89	0.031719122202662604
90-91	0.03376746057852305
92-93	0.03635800617152304
94-95	0.039701617343883486
96-97	0.03485187501280211
98-99	0.03235169728932538
100	0.029761151696325403
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1659882.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.44118473579744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.42508409527676	53.67505668485286
2	13.175270448870307	18.03462237470451
3	4.20445419811401	8.6327347945896
4	1.7750680607564608	4.859510442593866
5	0.8559748528635341	2.9291966517015084
6	0.475595650148123	1.9530197850797626
7	0.30375698714892935	1.4552641650574385
8	0.19298657855319215	1.0566584059430804
9	0.12593441995143978	0.7757190810442784
>10	0.456923590080847	4.916759026861967
>50	0.006307641213400878	0.29759412466770024
>100	0.0017623516332611337	0.19283359818770432
>500	2.202813474339294E-4	0.14209428200945326
>1k	5.507033685848235E-4	0.5457626534474491
>5k	1.101406737169647E-4	0.5331739292588132
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	7073	0.42611462742532297	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	1943	0.11705651365578999	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	1879	0.11320081788946443	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	6.024524634883685E-5
5	0.0	0.0	0.0	0.0	6.024524634883685E-5
6	0.0	0.0	0.0	0.0	6.024524634883685E-5
7	0.0	0.0	0.0	0.0	6.024524634883685E-5
8	0.0	0.0	0.0	0.0	6.024524634883685E-5
9	0.0	0.0	0.0	0.0	1.204904926976737E-4
10-11	0.0	0.0	0.0	0.0	1.204904926976737E-4
12-13	0.0	0.0	0.0	0.0	1.204904926976737E-4
14-15	0.0	0.0	0.0	0.0	1.506131158720921E-4
16-17	0.0	0.0	0.0	0.0	2.1085836222092894E-4
18-19	0.0	0.0	0.0	0.0	2.409809853953474E-4
20-21	0.0	0.0	0.0	9.036786952325528E-5	2.409809853953474E-4
22-23	0.0	0.0	0.0	2.4098098539534736E-4	2.711036085697658E-4
24-25	0.0	0.0	0.0	7.229429561860421E-4	3.313488549186026E-4
26-27	0.0	0.0	0.0	0.0017471121441162687	3.6147147809302105E-4
28-29	0.0	0.0	0.0	0.005241336432348805	3.6147147809302105E-4
30-31	0.0	0.0	0.0	0.014669717485941772	3.6147147809302105E-4
32-33	0.0	0.0	0.0	0.03455064878105793	3.6147147809302105E-4
34-35	0.0	0.0	0.0	0.06626977098372053	3.6147147809302105E-4
36-37	0.0	0.0	0.0	0.11431535494691791	3.6147147809302105E-4
38-39	0.0	0.0	0.0	0.1826334643064989	3.6147147809302105E-4
40-41	0.0	0.0	0.0	0.24992740447814965	3.6147147809302105E-4
42-43	0.0	0.0	0.0	0.3180346554755097	3.6147147809302105E-4
44-45	0.0	0.0	0.0	0.3999681905099278	3.6147147809302105E-4
46-47	0.0	0.0	0.0	0.5022646188102529	4.2171672444185794E-4
48-49	0.0	0.0	0.0	0.6028440575896359	5.422072171395316E-4
50-51	0.0	0.0	0.0	0.7095082662502515	5.422072171395316E-4
52-53	0.0	0.0	0.0	0.8081598571464719	6.928203330116237E-4
54-55	0.0	0.0	0.0	0.9128058500544014	8.133108257092974E-4
56-57	0.0	0.0	0.0	1.0361579919536448	8.735560720581344E-4
58-59	0.0	0.0	0.0	1.1693301090077488	9.338013184069711E-4
60-61	0.0	0.0	0.0	1.3117197487532246	9.639239415813896E-4
62-63	0.0	0.0	0.0	1.452422521600933	0.0010241691879302264
64-65	0.0	0.0	0.0	1.5896009475372346	0.0010241691879302264
66-67	0.0	0.0	0.0	1.745425277218501	0.0010241691879302264
68-69	0.0	0.0	0.0	1.9226969145999535	0.0010241691879302264
70-71	0.0	0.0	0.0	2.107047368427394	0.0010844144342790631
72-73	0.0	0.0	0.0	2.28570464647487	0.0012651501733255738
74-75	0.0	0.0	0.0	2.4649944996090083	0.0012651501733255738
76-77	0.0	0.0	0.0	2.6546465351151465	0.0012651501733255738
78-79	0.0	0.0	0.0	2.869571451464622	0.0012651501733255738
80-81	0.0	0.0	0.0	3.0982925292279813	0.0013856406660232475
82-83	0.0	0.0	0.0	3.334423772292247	0.0016266216514185948
84-85	0.0	0.0	0.0	3.559680748390548	0.0016266216514185948
86-87	0.0	0.0	0.0	3.79346242684721	0.0016266216514185948
88	0.0	0.0	0.0	3.97937925707972	0.0016266216514185948
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	5565	0.0	26.271362	1
TACATGG	5430	0.0	25.789728	2
ACATGGG	5450	0.0	23.970585	3
CATGGGG	4130	0.0	23.211887	4
CTAACGC	170	3.4580244E-6	22.11421	3
TCTAACG	180	5.6748413E-6	20.885643	2
TTACGTA	135	4.0823864E-4	20.885641	4
TAGTACT	745	0.0	20.18478	4
ATGGGGG	2360	0.0	20.111279	5
GTACTAG	655	0.0	20.095745	1
GAGTACA	4195	0.0	17.369493	1
GTATATA	860	0.0	16.945354	1
CTAGTAC	760	0.0	16.694773	3
GTATAAT	690	0.0	16.351196	1
AGTACAT	4115	0.0	16.330389	2
AGAGTAC	4005	0.0	16.308592	10-11
GTATTAA	1170	0.0	16.07169	1
ACGTGCG	295	1.3778736E-6	15.928767	9
CCTAGTA	840	0.0	15.664231	2
TTAGTAC	420	1.0040822E-9	15.664231	3
>>END_MODULE