FastQCFastQC Report
Mon 16 Jan 2017
ERR1146497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659882
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT74570.4492488020232764No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59440.3580977442974862No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53030.3194805413878818No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30470.18356726562490586No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21080.12699697930334805No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT19510.1175384756265807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG6000.024.309161
GTACATG52800.024.0596521
TACATGG51500.023.385842
GAGTACT72750.022.44832212-13
ACATGGG52000.022.1510833
GTACTTT76150.021.07574314-15
ATAAGCG1150.00395227920.4411053
AGAGTAC97150.020.36579510-11
CATGGGG38150.020.0874714
GGTATCA115400.019.7740421
ACGTGCG2201.4901889E-619.2256899
GTATCAA143000.019.1819171
CTAGTAC6150.019.1116033
AGTACTT74450.019.06357412-13
TATTCCG1250.006400899518.8058175
ACTTTTT87900.018.49904416-17
ATGGGGG22900.018.4773315
TTGTACG1600.001269836617.6437441
GGACCGT1600.001279366417.6240796
TAGTACT6750.017.4127924
Produced by FastQC (version 0.11.5)