##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146497_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1659882
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46588251454019	34.0	31.0	34.0	31.0	34.0
2	32.49068668736693	34.0	31.0	34.0	31.0	34.0
3	32.642168539691376	34.0	31.0	34.0	31.0	34.0
4	36.145315148908175	37.0	35.0	37.0	35.0	37.0
5	36.17962361179891	37.0	35.0	37.0	35.0	37.0
6	36.28066693897518	37.0	37.0	37.0	35.0	37.0
7	36.26140171409775	37.0	37.0	37.0	35.0	37.0
8	36.26791422522806	37.0	37.0	37.0	35.0	37.0
9	38.07145266952711	39.0	38.0	39.0	37.0	39.0
10-11	38.04587585141594	39.0	38.0	39.0	36.0	39.0
12-13	38.03898228910248	39.0	38.0	39.0	35.0	39.0
14-15	39.48827838364414	41.0	39.0	41.0	37.0	41.0
16-17	39.487354824017615	41.0	39.0	41.0	37.0	41.0
18-19	39.54417271830166	41.0	39.5	41.0	37.0	41.0
20-21	39.57885741275585	41.0	40.0	41.0	37.0	41.0
22-23	39.54357478423165	41.0	39.5	41.0	37.0	41.0
24-25	39.53553867082118	41.0	39.0	41.0	37.0	41.0
26-27	39.462744339657874	41.0	39.0	41.0	36.0	41.0
28-29	39.379038088249644	41.0	39.0	41.0	36.0	41.0
30-31	39.28117179413958	41.0	39.0	41.0	36.0	41.0
32-33	39.154491102379566	41.0	39.0	41.0	35.0	41.0
34-35	39.07416973013744	41.0	39.0	41.0	35.0	41.0
36-37	38.9868773804403	41.0	39.0	41.0	35.0	41.0
38-39	38.88800709929983	41.0	39.0	41.0	35.0	41.0
40-41	38.810880532471586	40.0	38.5	41.0	35.0	41.0
42-43	38.71780343422002	40.0	38.0	41.0	35.0	41.0
44-45	38.57980085331367	40.0	38.0	41.0	35.0	41.0
46-47	38.34920042509046	40.0	38.0	41.0	34.5	41.0
48-49	38.14184080555124	40.0	38.0	41.0	34.0	41.0
50-51	38.00336921540206	40.0	38.0	41.0	33.5	41.0
52-53	37.892497478736445	40.0	37.0	41.0	33.0	41.0
54-55	37.75781139864159	40.0	37.0	41.0	33.0	41.0
56-57	37.566207718379985	40.0	36.5	41.0	33.0	41.0
58-59	37.367964108292036	40.0	36.0	41.0	33.0	41.0
60-61	37.16445626857813	39.0	35.5	41.0	33.0	41.0
62-63	36.92308158049789	39.0	35.0	41.0	32.0	41.0
64-65	36.63015654124811	38.5	35.0	41.0	32.0	41.0
66-67	36.35475594048252	37.5	35.0	40.0	32.0	41.0
68-69	36.01794314294631	37.0	35.0	40.0	31.5	41.0
70-71	35.702821947584226	36.5	35.0	39.0	31.0	41.0
72-73	35.36057683618474	36.0	35.0	39.0	31.0	41.0
74-75	35.03327254587977	35.5	35.0	38.5	31.0	40.5
76-77	34.72587479109961	35.0	35.0	37.0	31.0	39.5
78-79	34.433518165749135	35.0	35.0	37.0	31.0	39.0
80-81	34.16110030713027	35.0	34.5	36.5	30.5	39.0
82-83	33.90721991081294	35.0	34.0	36.0	30.0	37.0
84-85	33.70661498829435	35.0	34.0	36.0	30.0	37.0
86-87	33.540260392003766	35.0	34.0	35.0	30.0	37.0
88-89	33.38668260755885	35.0	34.0	35.0	30.0	36.0
90-91	33.29756723670719	35.0	34.0	35.0	30.0	36.0
92-93	33.21395767891934	35.0	34.0	35.0	30.0	36.0
94-95	33.13799685760795	35.0	34.0	35.0	30.0	36.0
96-97	33.091026952518305	35.0	34.0	35.0	29.0	35.5
98-99	33.04575084252977	35.0	34.0	35.0	29.0	35.0
100	32.4987179811577	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.25185186631541967
1101	2	0.2053519397047836
1101	3	0.25128652740924196
1101	4	0.23206982400767373
1101	5	0.16965276769772686
1101	6	0.10620657562802904
1101	7	0.16204251970749084
1101	8	0.14612413757197373
1101	9	0.15858282450448513
1101	10-11	0.20249731112106417
1101	12-13	0.20178307554244412
1101	14-15	0.25947430190357323
1101	16-17	0.2689582668935415
1101	18-19	0.25733063800408473
1101	20-21	0.30269745779381907
1101	22-23	0.2850244802229085
1101	24-25	0.16752653362668468
1101	26-27	0.25689441838985516
1101	28-29	0.31134625203387145
1101	30-31	0.32266936778997746
1101	32-33	0.258836822450796
1101	34-35	0.2383844256746528
1101	36-37	0.3421771768358326
1101	38-39	0.3137856579530265
1101	40-41	0.2487915024211489
1101	42-43	0.2152099902338307
1101	44-45	0.21632189982117467
1101	46-47	0.22402779129252792
1101	48-49	0.23561367731208094
1101	50-51	0.26268131926811833
1101	52-53	0.28249821934352326
1101	54-55	0.2627413833210923
1101	56-57	0.3034228997213617
1101	58-59	0.3055542296091076
1101	60-61	0.2976114294539798
1101	62-63	0.2414311372508493
1101	64-65	0.26090510531270183
1101	66-67	0.196101283001056
1101	68-69	0.16737316455957085
1101	70-71	0.15177207134769333
1101	72-73	0.12277144110839089
1101	74-75	0.18309688303977367
1101	76-77	0.1346468630197606
1101	78-79	0.14392557862397126
1101	80-81	0.06437839350493135
1101	82-83	0.09700189420864547
1101	84-85	0.12274605107241143
1101	86-87	0.1567818057571131
1101	88-89	0.20080660273014672
1101	90-91	0.11027520307744254
1101	92-93	0.10050113061182486
1101	94-95	0.08114709822883981
1101	96-97	0.06934343040604318
1101	98-99	0.11540025265743026
1101	100	0.20678615040636572
1102	1	0.20647085547598465
1102	2	0.23737303398601028
1102	3	0.22171638184762799
1102	4	0.17812741484138428
1102	5	0.06829560791838674
1102	6	0.1612240054855434
1102	7	0.14277214308103225
1102	8	0.18297513305836333
1102	9	0.1710566335667778
1102	10-11	0.19524999790360198
1102	12-13	0.23639295748708378
1102	14-15	0.3164507062147308
1102	16-17	0.31525369144056725
1102	18-19	0.3238730034803581
1102	20-21	0.30568376312553625
1102	22-23	0.3184504240966035
1102	24-25	0.3324668290573811
1102	26-27	0.3175493995139149
1102	28-29	0.37118589119121026
1102	30-31	0.3537880477359465
1102	32-33	0.256564018259013
1102	34-35	0.28185169046387415
1102	36-37	0.3026103234097022
1102	38-39	0.31684395951879907
1102	40-41	0.36371256808848074
1102	42-43	0.3004116873758278
1102	44-45	0.30121745913037756
1102	46-47	0.24508453601664826
1102	48-49	0.23889012131347442
1102	50-51	0.21130985254484358
1102	52-53	0.26774606791759936
1102	54-55	0.3108530205585325
1102	56-57	0.2458880630820488
1102	58-59	0.2731839228251616
1102	60-61	0.3040030951207058
1102	62-63	0.3508604901770056
1102	64-65	0.3376094007803232
1102	66-67	0.3463251174466393
1102	68-69	0.3671996351749627
1102	70-71	0.2961777485891872
1102	72-73	0.30076797667422994
1102	74-75	0.2865234730335331
1102	76-77	0.2652720303187053
1102	78-79	0.3065281501817694
1102	80-81	0.2794828341957185
1102	82-83	0.29485400655968874
1102	84-85	0.24260808905812326
1102	86-87	0.223167256364313
1102	88-89	0.3294411049254151
1102	90-91	0.2602445892941674
1102	92-93	0.2739797486533391
1102	94-95	0.19089274230283593
1102	96-97	0.1713334801572799
1102	98-99	0.09776917489842418
1102	100	0.32604883512747307
1103	1	0.16829363427943633
1103	2	0.11301175743486169
1103	3	0.13847167173724273
1103	4	0.08557250701855423
1103	5	0.11462135622603142
1103	6	0.08032147339632445
1103	7	0.0864588731636502
1103	8	0.06953520337179953
1103	9	0.1030621315130631
1103	10-11	0.16009308019224022
1103	12-13	0.2131674609315155
1103	14-15	0.3103302431576438
1103	16-17	0.2865832522000247
1103	18-19	0.24813767119988484
1103	20-21	0.2423180084213996
1103	22-23	0.26590231355975646
1103	24-25	0.2561778145496305
1103	26-27	0.2254090018927286
1103	28-29	0.24162855842859443
1103	30-31	0.28543534554424355
1103	32-33	0.3102835183939803
1103	34-35	0.30576873872640675
1103	36-37	0.33124228744660655
1103	38-39	0.3391599784093202
1103	40-41	0.3214325275211891
1103	42-43	0.3314269097294371
1103	44-45	0.36582804698539917
1103	46-47	0.3570167186608444
1103	48-49	0.3749357360720751
1103	50-51	0.39661424851720994
1103	52-53	0.4359405207951852
1103	54-55	0.4044849334955529
1103	56-57	0.43406126642530296
1103	58-59	0.43768545752488563
1103	60-61	0.3357788039211158
1103	62-63	0.37873076541698936
1103	64-65	0.4096162453587411
1103	66-67	0.3380695811754393
1103	68-69	0.3183441422284474
1103	70-71	0.252245378378376
1103	72-73	0.3852598244398635
1103	74-75	0.33552354858118605
1103	76-77	0.2644826874691759
1103	78-79	0.23288901512839288
1103	80-81	0.3250479958607144
1103	82-83	0.34515231964243753
1103	84-85	0.3233647360002365
1103	86-87	0.30583423724513636
1103	88-89	0.33788871662725484
1103	90-91	0.3754709106619103
1103	92-93	0.35247156876493335
1103	94-95	0.3234064536029422
1103	96-97	0.2921317925386617
1103	98-99	0.39231058553966136
1103	100	0.29568508614370614
1104	1	0.06180531132723388
1104	2	0.056936471298946856
1104	3	0.09731636310088732
1104	4	0.1061875811919819
1104	5	0.01583256508705233
1104	6	0.06350493445484773
1104	7	0.0809873624578401
1104	8	0.07912793723842526
1104	9	0.10129210703805569
1104	10-11	0.11575736380824253
1104	12-13	0.09137124442415256
1104	14-15	0.1878824919136335
1104	16-17	0.19831112008199625
1104	18-19	0.17674609303755062
1104	20-21	0.153150972533048
1104	22-23	0.16075494651781952
1104	24-25	0.1333665961330155
1104	26-27	0.19404547089173008
1104	28-29	0.2700039133573213
1104	30-31	0.1825594850275465
1104	32-33	0.21350168193573182
1104	34-35	0.19527397172053185
1104	36-37	0.1670149412030213
1104	38-39	0.2095847245608624
1104	40-41	0.11798488041823418
1104	42-43	0.15022690629540136
1104	44-45	0.1769010767320367
1104	46-47	0.23666765206694151
1104	48-49	0.1538791076066346
1104	50-51	0.2325345287350018
1104	52-53	0.28125038519160483
1104	54-55	0.2281897305903584
1104	56-57	0.22413270568812038
1104	58-59	0.25135902723723547
1104	60-61	0.19597589177920582
1104	62-63	0.17524625835686436
1104	64-65	0.2433012257940348
1104	66-67	0.1891501284870145
1104	68-69	0.28959720160488445
1104	70-71	0.30467722105149164
1104	72-73	0.24847401643787492
1104	74-75	0.2983928806862295
1104	76-77	0.19651637914972042
1104	78-79	0.21440537964294748
1104	80-81	0.135756531954911
1104	82-83	0.18361537700698705
1104	84-85	0.16090452142489653
1104	86-87	0.16376868363097685
1104	88-89	0.24015225393642936
1104	90-91	0.10496846931963688
1104	92-93	0.17648729592884393
1104	94-95	0.13797436718752465
1104	96-97	0.09033472498621364
1104	98-99	0.20463004173678456
1104	100	0.18302705957542287
1105	1	0.11657823796373634
1105	2	0.10100147099496581
1105	3	0.13778067145861428
1105	4	0.04357039674535912
1105	5	0.032322894167556626
1105	6	0.10975147124950269
1105	7	0.10530735828896809
1105	8	0.0696335000798598
1105	9	0.0859718419671367
1105	10-11	0.11388988189507643
1105	12-13	0.14459673708954313
1105	14-15	0.20280506376158058
1105	16-17	0.17239609563328884
1105	18-19	0.17351877648972192
1105	20-21	0.20418190036810557
1105	22-23	0.18076658462479855
1105	24-25	0.11874704932436941
1105	26-27	0.12925152618142732
1105	28-29	0.14091307532676467
1105	30-31	0.20842526465044386
1105	32-33	0.23199576300295632
1105	34-35	0.2396453836660939
1105	36-37	0.18333808106006444
1105	38-39	0.23857047222238492
1105	40-41	0.19022825581666325
1105	42-43	0.19870205372589567
1105	44-45	0.1365898007959956
1105	46-47	0.10639354859200978
1105	48-49	0.033876762614930556
1105	50-51	0.14205528191159544
1105	52-53	0.15880464569413277
1105	54-55	0.15878692181140508
1105	56-57	0.010632848993168409
1105	58-59	0.08235391168125972
1105	60-61	0.14702957471895672
1105	62-63	0.13233325754914205
1105	64-65	0.08575069990792628
1105	66-67	0.0476392590162007
1105	68-69	0.11854786124236938
1105	70-71	0.040487502537345677
1105	72-73	0.08786249471196328
1105	74-75	0.08143103456979617
1105	76-77	0.023629260102303817
1105	78-79	0.13616907396024658
1105	80-81	0.12376684991842524
1105	82-83	0.10698295879942776
1105	84-85	0.10448436839247677
1105	86-87	0.07212276000822015
1105	88-89	0.08880622869737209
1105	90-91	0.07042014077760683
1105	92-93	0.04665022942661068
1105	94-95	0.08111109757427926
1105	96-97	0.01731593899710049
1105	98-99	0.020959782311216202
1105	100	0.1948105560449065
1106	1	0.11190532093453953
1106	2	0.1000202008613158
1106	3	0.09367512549977874
1106	4	0.04161193095611537
1106	5	0.055850628206002284
1106	6	-0.033346575123147204
1106	7	0.032227519740160915
1106	8	0.026662866031657018
1106	9	0.1157550882226488
1106	10-11	0.09015112384692259
1106	12-13	0.11320262895459621
1106	14-15	0.20124470843313702
1106	16-17	0.10376388943074488
1106	18-19	0.0780413622553695
1106	20-21	0.034069045814234755
1106	22-23	0.1657481433673169
1106	24-25	0.11208468349565948
1106	26-27	0.10931850041459512
1106	28-29	0.15156987270211886
1106	30-31	0.15675485597179772
1106	32-33	0.1739158607904301
1106	34-35	0.19013215646725712
1106	36-37	0.1828497378871532
1106	38-39	0.15725119017937317
1106	40-41	0.1951526020963641
1106	42-43	0.09220555310835721
1106	44-45	0.06253531314039407
1106	46-47	0.13466643676032675
1106	48-49	0.12930825408611213
1106	50-51	0.07450799037845002
1106	52-53	0.15767253498716371
1106	54-55	0.03459275595467659
1106	56-57	0.07329161569958842
1106	58-59	0.025202396272511862
1106	60-61	0.04830183049432435
1106	62-63	0.0517384686368203
1106	64-65	0.05450166016903779
1106	66-67	-0.02611244398830337
1106	68-69	-0.1005109343249373
1106	70-71	-0.09991357889704489
1106	72-73	-0.07880148393098096
1106	74-75	-0.02189373953010687
1106	76-77	-0.06386124339282873
1106	78-79	-0.0905879385984818
1106	80-81	-0.05621405096565013
1106	82-83	0.01843928760980873
1106	84-85	0.011133067742676417
1106	86-87	0.07974899455386009
1106	88-89	0.059598842008185215
1106	90-91	0.02803546760592468
1106	92-93	-0.006489792558589613
1106	94-95	-0.04750209439730213
1106	96-97	-0.027129740995086138
1106	98-99	-0.04523087779666923
1106	100	-0.049662711559172124
1107	1	0.07152362941009471
1107	2	0.08175884570150771
1107	3	0.11033145230989305
1107	4	0.039242541279399745
1107	5	0.05716315138915462
1107	6	-0.008913426168071226
1107	7	8.427262587531459E-4
1107	8	0.01680508321755525
1107	9	0.06719466528039675
1107	10-11	0.04734093834093045
1107	12-13	0.023643384022278724
1107	14-15	0.16349711234788344
1107	16-17	0.1350928395344866
1107	18-19	0.14505700247727304
1107	20-21	0.07958661609740858
1107	22-23	0.12809535873932276
1107	24-25	0.15139173647349935
1107	26-27	0.1702681639276733
1107	28-29	0.14948100428460265
1107	30-31	0.08914385197309116
1107	32-33	0.13342068900071524
1107	34-35	0.08729262261679338
1107	36-37	0.11575208534708992
1107	38-39	0.16228793675234243
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2216	38-39	-0.6193751425990754
2216	40-41	-0.6281581180067306
2216	42-43	-0.6347037433755531
2216	44-45	-0.5858963787946223
2216	46-47	-0.6100400387380347
2216	48-49	-0.6722340994797946
2216	50-51	-0.6374505450718146
2216	52-53	-0.6210608854578226
2216	54-55	-0.5465260917848909
2216	56-57	-0.5597586047644896
2216	58-59	-0.5357661391180883
2216	60-61	-0.5856467288818195
2216	62-63	-0.5952653529394283
2216	64-65	-0.5728373771875468
2216	66-67	-0.5531186223523932
2216	68-69	-0.5213912542514336
2216	70-71	-0.5399894883486525
2216	72-73	-0.5855052028825369
2216	74-75	-0.46434963179805067
2216	76-77	-0.4768992771754412
2216	78-79	-0.5714470921834831
2216	80-81	-0.4797801394734762
2216	82-83	-0.4928642696482015
2216	84-85	-0.46841903815167996
2216	86-87	-0.47510718172194544
2216	88-89	-0.49900977309554406
2216	90-91	-0.5299914833072563
2216	92-93	-0.540381744277262
2216	94-95	-0.5676192923853804
2216	96-97	-0.5324681637968567
2216	98-99	-0.5762171189288807
2216	100	-0.7159290226193669
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	13.0
8	41.0
9	68.0
10	113.0
11	123.0
12	117.0
13	104.0
14	160.0
15	270.0
16	503.0
17	962.0
18	1724.0
19	2616.0
20	3990.0
21	5803.0
22	8724.0
23	11070.0
24	11519.0
25	10104.0
26	9154.0
27	9264.0
28	10837.0
29	13198.0
30	16348.0
31	21063.0
32	27238.0
33	36408.0
34	52687.0
35	85303.0
36	157767.0
37	386868.0
38	573606.0
39	201519.0
40	597.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.81479821276227	19.227506494883624	13.662511869128696	23.295183423225417
2	15.570704342205543	21.06593138910059	39.07207183306482	24.291292435629046
3	18.682227659037387	25.90240120201678	30.791438160779382	24.623932978166454
4	12.423593966318087	17.49250850361652	38.68907548849858	31.394822041566812
5	12.71550607087367	38.42200678144252	35.04538841417184	13.81709873351197
6	29.996342952713235	38.27868146274662	17.48610834138145	14.238867243158701
7	26.10150968653777	33.43004709373744	22.591679764995202	17.876763454729584
8	25.79111045242975	34.318825073107604	20.29108093225904	19.5989835422036
9	26.024500536785144	15.477003787016185	20.723641801043687	37.77485387515498
10-11	24.608827615457002	25.91325768940202	28.467144049998733	21.010770645142244
12-13	26.08573982969874	24.040684819764298	28.168237260238982	21.705338090297985
14-15	22.876505679319372	25.16886742551579	26.897544524249312	25.057082370915523
16-17	21.762842177937948	28.05307847184318	27.365860946742	22.818218403476873
18-19	21.662383229651265	27.146688740524926	29.569993529660543	21.620934500163262
20-21	22.699956984894108	25.832047097323784	29.528394187056673	21.939601730725435
22-23	22.480779408792294	25.706808380277174	29.457937607896024	22.354474603034507
24-25	22.592027626060165	25.964767375030274	29.47944492439824	21.96376007451132
26-27	22.208807614035216	25.79626142099258	29.864502416436835	22.130428548535377
28-29	21.918723040219774	26.653191796997373	29.516501192857312	21.911583969925534
30-31	22.32981370033186	25.836574281446335	29.37011391489563	22.463498103326174
32-33	22.326361798842665	26.164310522970517	29.176911097120517	22.332416581066305
34-35	22.89072064082667	25.97580753993117	29.252989415949337	21.880482403292817
36-37	22.66454320464037	25.650202305243546	29.568947651259016	22.116306838857067
38-39	22.437283284268084	26.166051506011485	29.324321352736927	22.072343856983505
40-41	22.485495677320237	26.151820947675997	29.13028285688466	22.232400518119107
42-43	22.34573613428064	26.143209064340695	28.73874834549997	22.772306455878695
44-45	22.56898569950834	26.039040336218495	28.72873406579901	22.66323989847416
46-47	23.171964902208085	26.348104813821994	28.203031972559405	22.276898311410516
48-49	22.96221747753436	26.507017425482026	27.978068135538486	22.552696961445125
50-51	22.46614716768226	26.776819970979627	28.164791367508933	22.592241493829178
52-53	22.839520862483678	26.976562747103287	27.560106816398395	22.623809574014636
54-55	22.43808792821537	26.584928553957155	27.827929444396915	23.149054073430555
56-57	23.00064734043691	26.472066913734434	27.54651949838195	22.98076624744671
58-59	23.20813941228744	26.4325154760291	27.66550690584851	22.69383820583495
60-61	22.710955501005664	26.726567788045465	28.156549415864664	22.40592729508421
62-63	22.49813237260974	26.665411540732354	27.981034545082117	22.855421541575797
64-65	22.241023065793346	26.91934036452081	27.843761475434775	22.99587509425107
66-67	22.58368576128806	26.453439595420036	27.601743708788224	23.36113093450368
68-69	22.49874759271203	26.527567871739034	27.774341962768485	23.199342572780452
70-71	22.836773509847593	26.386950122511294	27.554708355649648	23.221568011991465
72-73	22.725487171824767	26.275557542427208	27.98834065155466	23.010614634193367
74-75	22.488805211432012	27.126798960725985	27.39917912414806	22.985216703693943
76-77	22.719213211624698	26.460090938064635	27.287293553302156	23.533402297008514
78-79	22.70891420281613	26.58678280441577	27.41582463183819	23.288478360929904
80-81	22.87733277265057	26.54163159889173	27.349731697633743	23.23130393082396
82-83	22.914591967245805	26.236942572320032	27.825823448576674	23.022642011857492
84-85	22.734917043626986	26.75882602250707	27.358387978397214	23.147868955468727
86-87	22.49007609581651	26.96289673777819	27.31515090539623	23.23187626100907
88-89	22.573188338785037	26.700869973314312	26.928627607382104	23.797314080518543
90-91	22.71034928988928	26.320786658328725	27.17928141879965	23.789582632982345
92-93	23.008352400953804	26.556014222697762	27.276667859522547	23.158965516825894
94-95	22.565369104550808	26.395641376917155	27.463307632711242	23.5756818858208
96-97	22.185127740002187	26.391858907490352	27.841817051941547	23.581196300565914
98-99	22.450301920689114	26.67047418688056	27.13647369407178	23.74275019835855
100	22.471954112041374	25.83727992992218	27.188229914529398	24.502536043507046
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	156.0
1	128.0
2	235.5
3	346.0
4	206.5
5	164.0
6	405.0
7	1078.5
8	2093.0
9	2530.5
10	1787.5
11	1001.5
12	906.5
13	998.5
14	1131.0
15	1286.5
16	1461.0
17	1707.5
18	1998.5
19	2239.0
20	2539.0
21	2899.0
22	3383.0
23	4071.0
24	5046.5
25	6472.0
26	8201.0
27	10264.5
28	12465.5
29	15250.0
30	18721.0
31	22089.5
32	25847.5
33	28595.0
34	30479.5
35	32887.0
36	35897.5
37	39897.5
38	42687.0
39	44284.0
40	45994.5
41	47490.0
42	48827.0
43	51215.0
44	54220.5
45	57748.0
46	60967.5
47	68054.5
48	80061.5
49	77452.0
50	65611.5
51	62207.5
52	61480.5
53	60405.5
54	59133.0
55	55219.0
56	50034.5
57	44625.5
58	39255.0
59	34365.5
60	28537.0
61	23183.0
62	18893.0
63	14324.5
64	10087.5
65	7194.0
66	5208.5
67	3907.5
68	2898.0
69	2012.0
70	1437.0
71	1091.5
72	837.0
73	686.5
74	486.0
75	299.5
76	235.0
77	172.0
78	110.0
79	62.0
80	31.5
81	21.5
82	14.0
83	6.5
84	4.5
85	3.5
86	2.5
87	2.5
88	1.5
89	0.0
90	0.0
91	0.5
92	1.0
93	1.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0068679580837674005
2	0.08253598749790649
3	3.0122623174418426E-4
4	0.0
5	0.03903891963404628
6	0.004397902983465089
7	6.024524634883685E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.120845939651132E-4
24-25	0.0
26-27	0.0
28-29	1.204904926976737E-4
30-31	1.5061311587209213E-4
32-33	0.002229074114906963
34-35	0.001566376405069758
36-37	8.434334488837159E-4
38-39	0.0010542918111046448
40-41	0.0019278478831627791
42-43	9.036786952325527E-4
44-45	5.422072171395317E-4
46-47	3.313488549186027E-4
48-49	8.434334488837159E-4
50-51	8.133108257092974E-4
52-53	0.0014760085355465028
54-55	0.0016868668977674318
56-57	8.133108257092974E-4
58-59	0.0034339790418837003
60-61	0.002138706245383708
62-63	0.0013253954196744107
64-65	0.0052112138091743865
66-67	0.0052112138091743865
68-69	0.004729251838383692
70-71	0.0021085836222092895
72-73	0.005904034142186011
74-75	0.00418704462124416
76-77	0.004277412490767416
78-79	8.434334488837159E-4
80-81	6.024524634883685E-5
82-83	1.8073573904651055E-4
84-85	2.1085836222092897E-4
86-87	6.024524634883685E-5
88-89	2.1085836222092897E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	3.915941012674395E-4
98-99	5.422072171395317E-4
100	7.83188202534879E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1659882.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.9699638360257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.38239310564137	49.625613177826175
2	14.340256956988782	18.63371951789754
3	4.577206593563184	8.921428405614552
4	1.9422483211070694	5.047512127316317
5	0.9871550632593075	3.206771438025343
6	0.5432447458277764	2.1176754894324965
7	0.3265564368937879	1.4851451926787564
8	0.2139611460588735	1.11208383374077
9	0.14516076465074704	0.8487980666791985
>10	0.5212530097835454	5.344704057500463
>50	0.013846277134064094	0.6163235869282132
>100	0.005327755291949978	0.6836290688910992
>500	3.4745594989694456E-4	0.134542234976522
>1k	6.949118997938891E-4	0.8146276486889416
>5k	3.4745594989694456E-4	1.4074261538036175
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	7457	0.4492488020232764	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5944	0.3580977442974862	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5303	0.3194805413878818	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3047	0.18356726562490586	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	2108	0.12699697930334805	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	1951	0.1175384756265807	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.024524634883685E-5
2	0.0	0.0	0.0	0.0	6.024524634883685E-5
3	0.0	0.0	0.0	0.0	6.024524634883685E-5
4	0.0	0.0	0.0	0.0	6.024524634883685E-5
5	0.0	0.0	0.0	0.0	6.024524634883685E-5
6	0.0	0.0	0.0	0.0	6.024524634883685E-5
7	0.0	0.0	0.0	0.0	6.024524634883685E-5
8	0.0	0.0	0.0	0.0	6.024524634883685E-5
9	0.0	0.0	0.0	0.0	1.204904926976737E-4
10-11	0.0	0.0	0.0	3.0122623174418424E-5	1.204904926976737E-4
12-13	0.0	0.0	0.0	6.024524634883685E-5	1.8073573904651055E-4
14-15	0.0	0.0	0.0	6.024524634883685E-5	2.711036085697658E-4
16-17	0.0	0.0	0.0	6.024524634883685E-5	3.012262317441842E-4
18-19	0.0	0.0	0.0	6.024524634883685E-5	3.6147147809302105E-4
20-21	0.0	0.0	0.0	1.506131158720921E-4	3.915941012674395E-4
22-23	0.0	0.0	0.0	3.012262317441842E-4	4.2171672444185794E-4
24-25	0.0	0.0	0.0	7.83188202534879E-4	4.819619707906948E-4
26-27	0.0	0.0	0.0	0.0018073573904651054	4.819619707906948E-4
28-29	0.0	0.0	0.0	0.005331704301872061	4.819619707906948E-4
30-31	0.0	0.0	0.0	0.01469984010911619	4.819619707906948E-4
32-33	0.0	0.0	0.0	0.03449040353470909	4.819619707906948E-4
34-35	0.0	0.0	0.0	0.0662095257373717	6.024524634883684E-4
36-37	0.0	0.0	0.0	0.11446596806279	6.024524634883684E-4
38-39	0.0	0.0	0.0	0.18320579414681284	6.024524634883684E-4
40-41	0.0	0.0	0.0	0.2510720641587776	6.024524634883684E-4
42-43	0.0	0.0	0.0	0.31945041876470737	6.024524634883684E-4
44-45	0.0	0.0	0.0	0.4015948121613464	6.024524634883684E-4
46-47	0.0	0.0	0.0	0.503861117838497	6.024524634883684E-4
48-49	0.0	0.0	0.0	0.6054346031826359	7.229429561860421E-4
50-51	0.0	0.0	0.0	0.7134543298861002	7.229429561860421E-4
52-53	0.0	0.0	0.0	0.8120155529127975	8.735560720581342E-4
54-55	0.0	0.0	0.0	0.9171435077915178	0.0010241691879302264
56-57	0.0	0.0	0.0	1.0401341782126683	0.0010241691879302264
58-59	0.0	0.0	0.0	1.1737882572375629	0.0010844144342790634
60-61	0.0	0.0	0.0	1.316629736330655	0.0011446596806279
62-63	0.0	0.0	0.0	1.4580253295113748	0.0012651501733255738
64-65	0.0	0.0	0.0	1.5962881698819555	0.0012651501733255738
66-67	0.0	0.0	0.0	1.7530463008816288	0.0013253954196744107
68-69	0.0	0.0	0.0	1.9318240694218023	0.0013856406660232475
70-71	0.0	0.0	0.0	2.11710832456765	0.0014458859123720842
72-73	0.0	0.0	0.0	2.297000630165277	0.0015061311587209211
74-75	0.0	0.0	0.0	2.477886982327659	0.0015061311587209211
76-77	0.0	0.0	0.0	2.669195762108391	0.0015061311587209211
78-79	0.0	0.0	0.0	2.885265338138494	0.0015061311587209211
80-81	0.0	0.0	0.0	3.1168179424802487	0.0015061311587209211
82-83	0.0	0.0	0.0	3.3551180144130726	0.0015061311587209211
84-85	0.0	0.0	0.0	3.581700385931048	0.0015061311587209211
86-87	0.0	0.0	0.0	3.8171388086623024	0.0015061311587209211
88	0.0	0.0	0.0	4.0048027510389295	0.0015061311587209211
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	600	0.0	24.30916	1
GTACATG	5280	0.0	24.059652	1
TACATGG	5150	0.0	23.38584	2
GAGTACT	7275	0.0	22.448322	12-13
ACATGGG	5200	0.0	22.151083	3
GTACTTT	7615	0.0	21.075743	14-15
ATAAGCG	115	0.003952279	20.441105	3
AGAGTAC	9715	0.0	20.365795	10-11
CATGGGG	3815	0.0	20.087471	4
GGTATCA	11540	0.0	19.774042	1
ACGTGCG	220	1.4901889E-6	19.225689	9
GTATCAA	14300	0.0	19.181917	1
CTAGTAC	615	0.0	19.111603	3
AGTACTT	7445	0.0	19.063574	12-13
TATTCCG	125	0.0064008995	18.805817	5
ACTTTTT	8790	0.0	18.499044	16-17
ATGGGGG	2290	0.0	18.477331	5
TTGTACG	160	0.0012698366	17.643744	1
GGACCGT	160	0.0012793664	17.624079	6
TAGTACT	675	0.0	17.412792	4
>>END_MODULE