FastQCFastQC Report
Mon 16 Jan 2017
ERR1146496_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146496_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2455966
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG88050.028.8356341
TACATGG92600.026.6446462
CATGGGG63400.024.9810754
ACATGGG94350.024.9051843
ATGGGGG38950.021.5981245
GTATCAA80650.019.8216441
GCATCGT8150.019.0283138
GTACTAG9700.018.9041861
GGTATCA63000.018.807241
CGCCGTA3157.763447E-917.90618794
CAACGCA86700.017.7255275
ATCAACG88250.017.5736053
TCAACGC89950.017.3463234
TATCAAC92300.017.1589152
GGGCTAT13800.017.0268736
CTAGTAC12750.016.9554483
AACGCAG90900.016.905496
GTAAGGT10950.016.738674
GCTATTG13500.016.7090368
GAGTACA81100.016.6389581
Produced by FastQC (version 0.11.5)