##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146496_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2455966
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.81241434124088	31.0	31.0	34.0	27.0	34.0
2	30.85361401582921	31.0	31.0	34.0	27.0	34.0
3	31.06850990608176	31.0	31.0	34.0	27.0	34.0
4	34.60648518749852	37.0	35.0	37.0	31.0	37.0
5	34.67785384651091	37.0	35.0	37.0	31.0	37.0
6	34.57084259309779	37.0	35.0	37.0	30.0	37.0
7	34.58166277546187	37.0	35.0	37.0	30.0	37.0
8	34.59637714854359	37.0	35.0	37.0	30.0	37.0
9	36.166480317724265	39.0	37.0	39.0	30.0	39.0
10-11	36.14909591582294	39.0	37.0	39.0	30.0	39.0
12-13	36.09679816414396	39.0	37.0	39.0	30.0	39.0
14-15	37.38649008170309	40.0	38.0	41.0	31.0	41.0
16-17	37.37749158579557	40.0	38.0	41.0	31.0	41.0
18-19	37.36511213917457	40.0	38.0	41.0	31.0	41.0
20-21	37.47797282209933	40.0	38.0	41.0	31.0	41.0
22-23	37.4787816688016	40.0	38.0	41.0	31.0	41.0
24-25	37.530540732241406	40.0	38.0	41.0	31.0	41.0
26-27	37.56777984711515	40.0	38.0	41.0	31.0	41.0
28-29	37.51637522669287	40.0	38.0	41.0	30.0	41.0
30-31	37.49495962077651	40.0	38.0	41.0	30.0	41.0
32-33	37.51600856852252	40.0	38.0	41.0	30.0	41.0
34-35	37.444091245562845	40.0	37.5	41.0	30.0	41.0
36-37	37.34238381150227	40.0	37.0	41.0	30.0	41.0
38-39	37.291390230972254	40.0	37.0	41.0	30.0	41.0
40-41	37.22422378811433	40.0	37.0	41.0	30.0	41.0
42-43	37.068094590886034	40.0	36.5	41.0	30.0	41.0
44-45	36.88985576347555	40.0	36.0	41.0	30.0	41.0
46-47	36.76196514935467	40.0	36.0	41.0	29.0	41.0
48-49	36.647015064540795	39.5	35.5	41.0	29.0	41.0
50-51	36.520423124750096	39.0	35.0	41.0	29.0	41.0
52-53	36.401333324647	39.0	35.0	41.0	29.0	41.0
54-55	36.226959168001514	39.0	35.0	41.0	28.0	41.0
56-57	36.097350289051235	39.0	35.0	40.0	28.0	41.0
58-59	35.886984795392124	38.5	35.0	40.0	28.0	41.0
60-61	35.70455047016124	38.0	34.5	40.0	28.0	41.0
62-63	35.47976132405742	37.5	34.0	40.0	28.0	41.0
64-65	35.234262404284095	37.0	34.0	40.0	28.0	41.0
66-67	34.971909423827526	36.5	34.0	39.5	27.5	41.0
68-69	34.68571490810541	36.0	34.0	39.0	27.0	41.0
70-71	34.41428790137974	35.5	34.0	39.0	27.0	41.0
72-73	34.103331031455646	35.0	33.0	38.5	26.0	40.0
74-75	33.805928298681664	35.0	33.0	37.0	26.0	39.5
76-77	33.511349709238644	35.0	33.0	37.0	26.0	39.0
78-79	33.244886533445495	35.0	33.0	36.5	26.0	39.0
80-81	32.978457967251984	35.0	33.0	36.0	26.0	38.5
82-83	32.74473140100474	35.0	33.0	36.0	26.0	37.0
84-85	32.54186173587094	35.0	33.0	35.0	25.5	37.0
86-87	32.36745989154573	35.0	32.5	35.0	25.0	36.5
88-89	32.22183185760715	35.0	32.0	35.0	25.0	36.0
90-91	32.073078983992446	35.0	32.0	35.0	25.0	36.0
92-93	31.992593545676122	35.0	32.0	35.0	25.0	36.0
94-95	31.906160753039742	35.0	32.0	35.0	25.0	35.0
96-97	31.842700591131962	35.0	32.0	35.0	25.0	35.0
98-99	31.77813658658141	35.0	32.0	35.0	24.0	35.0
100	30.79413884394165	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.34372983176362837
1101	2	0.4066588364363355
1101	3	0.5048811339128072
1101	4	0.4041815463516514
1101	5	0.4155108284498752
1101	6	0.5164697356640033
1101	7	0.5351632959045389
1101	8	0.5225329319395584
1101	9	0.7339728581574576
1101	10-11	0.7356427130787324
1101	12-13	0.6717812089959239
1101	14-15	0.7450870783408376
1101	16-17	0.7588273340984131
1101	18-19	0.6855209656251944
1101	20-21	0.7278511850234253
1101	22-23	0.7975387483127605
1101	24-25	0.8040652454166022
1101	26-27	0.7054772191389844
1101	28-29	0.7788052158720902
1101	30-31	0.7351048304655023
1101	32-33	0.7599699527840116
1101	34-35	0.7688251135683899
1101	36-37	0.7969471200864362
1101	38-39	0.7924167435369682
1101	40-41	0.7594001495149172
1101	42-43	0.7308204871016102
1101	44-45	0.6696043496360957
1101	46-47	0.6926460334621183
1101	48-49	0.7092790873899446
1101	50-51	0.6084031130469754
1101	52-53	0.6412540591160507
1101	54-55	0.5969869498262383
1101	56-57	0.583690288972754
1101	58-59	0.6485034154309943
1101	60-61	0.6856528859269986
1101	62-63	0.6921103400499504
1101	64-65	0.6569488737453284
1101	66-67	0.5351213541072468
1101	68-69	0.5313284701549961
1101	70-71	0.5504342982773522
1101	72-73	0.5584281732167611
1101	74-75	0.5650102213340276
1101	76-77	0.482895899717775
1101	78-79	0.5021775999205715
1101	80-81	0.5221461518406372
1101	82-83	0.42582548574256407
1101	84-85	0.5416621128360006
1101	86-87	0.4776142434350703
1101	88-89	0.509920101959338
1101	90-91	0.5599615283346395
1101	92-93	0.5416359024308832
1101	94-95	0.47246107197352316
1101	96-97	0.43516868848609036
1101	98-99	0.39289931487474306
1101	100	0.2777912814554284
1102	1	0.3319339297949675
1102	2	0.28524624005989807
1102	3	0.36586288217659657
1102	4	0.2966412260853417
1102	5	0.3784850579166701
1102	6	0.30586191841094745
1102	7	0.29578077471607145
1102	8	0.3657847638093088
1102	9	0.4415674170738271
1102	10-11	0.5012580395628845
1102	12-13	0.48592592192327544
1102	14-15	0.5368721708430826
1102	16-17	0.5774046935275265
1102	18-19	0.5398643622847743
1102	20-21	0.49289824924886716
1102	22-23	0.4437198499217416
1102	24-25	0.5241672657345688
1102	26-27	0.49953086470327435
1102	28-29	0.5579501679469274
1102	30-31	0.5558029019801722
1102	32-33	0.5348588448615317
1102	34-35	0.493001967918012
1102	36-37	0.5108428804797853
1102	38-39	0.4984271894992034
1102	40-41	0.5591412962749445
1102	42-43	0.5111934615223959
1102	44-45	0.3575093698201357
1102	46-47	0.3781488171388645
1102	48-49	0.4293663762227169
1102	50-51	0.36408544865699355
1102	52-53	0.4142728656744339
1102	54-55	0.42857627619559935
1102	56-57	0.42260274186070035
1102	58-59	0.3924233297205362
1102	60-61	0.4150163709755077
1102	62-63	0.3414822909194939
1102	64-65	0.3593437445100278
1102	66-67	0.34161530845877053
1102	68-69	0.37241706951599696
1102	70-71	0.3331236074280923
1102	72-73	0.316386044995447
1102	74-75	0.29457248577374884
1102	76-77	0.4112816541802715
1102	78-79	0.3200823331276368
1102	80-81	0.3145262432906364
1102	82-83	0.3242993421392839
1102	84-85	0.3732673185985007
1102	86-87	0.33602405613476094
1102	88-89	0.3248971438005839
1102	90-91	0.29431611709662064
1102	92-93	0.29407338927777715
1102	94-95	0.1855813727254052
1102	96-97	0.2704064489177007
1102	98-99	0.33306164818894857
1102	100	0.33321629436939304
1103	1	0.2728900828974439
1103	2	0.23773265714265435
1103	3	0.23702326476892566
1103	4	0.30537473170694795
1103	5	0.2732194975467195
1103	6	0.2703403367296886
1103	7	0.3559795499711669
1103	8	0.30550083928842753
1103	9	0.4444899973716332
1103	10-11	0.5056680985294832
1103	12-13	0.49346411912312504
1103	14-15	0.5591839119612203
1103	16-17	0.46567481157777024
1103	18-19	0.4083690241339326
1103	20-21	0.39626495173089893
1103	22-23	0.43395840831833965
1103	24-25	0.4355794033966518
1103	26-27	0.5223982706177281
1103	28-29	0.5451700239364072
1103	30-31	0.4614913118483557
1103	32-33	0.42693741744381697
1103	34-35	0.4353638154288362
1103	36-37	0.33486934054097617
1103	38-39	0.3290790524832161
1103	40-41	0.34125690732480507
1103	42-43	0.3607183227671271
1103	44-45	0.30829938529331
1103	46-47	0.3033628900008907
1103	48-49	0.2919590162830161
1103	50-51	0.2400847089410405
1103	52-53	0.2624075407963318
1103	54-55	0.2909874985762144
1103	56-57	0.18323879224566042
1103	58-59	0.29903360474973795
1103	60-61	0.22799932239536247
1103	62-63	0.24964705211530003
1103	64-65	0.2916660254101373
1103	66-67	0.2550874146126887
1103	68-69	0.2571395941010195
1103	70-71	0.2718592959140409
1103	72-73	0.1880078722343157
1103	74-75	0.16724829120972373
1103	76-77	0.2630900373156777
1103	78-79	0.25143344422313874
1103	80-81	0.2872582882073118
1103	82-83	0.24412735817894315
1103	84-85	0.35857380328567956
1103	86-87	0.34047244690141554
1103	88-89	0.21539196930018534
1103	90-91	0.2660009138268933
1103	92-93	0.2284900202461202
1103	94-95	0.27784601547302756
1103	96-97	0.1465445522485318
1103	98-99	0.14396151499325782
1103	100	0.09628304905289653
1104	1	0.010667980054062554
1104	2	0.056781222693949474
1104	3	0.20877005329154485
1104	4	0.13195938510913408
1104	5	0.0874871195425655
1104	6	0.13208468544721796
1104	7	0.20928656026983106
1104	8	0.1273603288991083
1104	9	0.25375552650716315
1104	10-11	0.26192337824529943
1104	12-13	0.19981589424919832
1104	14-15	0.2733764148207243
1104	16-17	0.36947987786659553
1104	18-19	0.2934919880718212
1104	20-21	0.3505349016141679
1104	22-23	0.3862045734595725
1104	24-25	0.264563571523837
1104	26-27	0.3543186658213884
1104	28-29	0.3864623148238451
1104	30-31	0.3030034090286975
1104	32-33	0.3211191762075387
1104	34-35	0.3220538057367719
1104	36-37	0.322428707266063
1104	38-39	0.29508289797416154
1104	40-41	0.3267564965869738
1104	42-43	0.38672521241855407
1104	44-45	0.24104389741063414
1104	46-47	0.21358231577373488
1104	48-49	0.2155476785759447
1104	50-51	0.28605197973925556
1104	52-53	0.19557819061267878
1104	54-55	0.29658741083277107
1104	56-57	0.23878715522597815
1104	58-59	0.2384765233833548
1104	60-61	0.241906659045938
1104	62-63	0.26422383546732675
1104	64-65	0.2904102644483686
1104	66-67	0.27867549493376487
1104	68-69	0.24832204899260546
1104	70-71	0.24245669002314685
1104	72-73	0.25386640382978953
1104	74-75	0.2353850635490531
1104	76-77	0.24445015030775608
1104	78-79	0.2538366746146039
1104	80-81	0.260343835831641
1104	82-83	0.27312365818095685
1104	84-85	0.19592806969896515
1104	86-87	0.1960446259607238
1104	88-89	0.22222678106586358
1104	90-91	0.20156078194311533
1104	92-93	0.31373842150228626
1104	94-95	0.23795363549912807
1104	96-97	0.15299866488694747
1104	98-99	0.1889149012860294
1104	100	0.044934687415583596
1105	1	0.1673752486098934
1105	2	0.09369792526261023
1105	3	0.15639489430927966
1105	4	0.20073067121124666
1105	5	0.2000464402837494
1105	6	0.14711627576460984
1105	7	0.23675152233052899
1105	8	0.1790573834241016
1105	9	0.24809660298974023
1105	10-11	0.24450584804124986
1105	12-13	0.20571529583686043
1105	14-15	0.3355882111502666
1105	16-17	0.3106735692853704
1105	18-19	0.32631442059840765
1105	20-21	0.3677365663672134
1105	22-23	0.3689224745019999
1105	24-25	0.3019321238524526
1105	26-27	0.43396324640710304
1105	28-29	0.38683888377525477
1105	30-31	0.32485170541905006
1105	32-33	0.3239354864996855
1105	34-35	0.37807274549769687
1105	36-37	0.29966747647019076
1105	38-39	0.37124312323940245
1105	40-41	0.28362558177673236
1105	42-43	0.3566107039597739
1105	44-45	0.22913266774851593
1105	46-47	0.2564773287293747
1105	48-49	0.1909325857220523
1105	50-51	0.23849809067743877
1105	52-53	0.21506087211012925
1105	54-55	0.27366139504688647
1105	56-57	0.24635807110812635
1105	58-59	0.15011907559241422
1105	60-61	0.18651857740789524
1105	62-63	0.11252706953251135
1105	64-65	0.11995575553608262
1105	66-67	0.13929913830226326
1105	68-69	0.25525913839577896
1105	70-71	0.1825895239580504
1105	72-73	0.09912628958930725
1105	74-75	0.1802581972466939
1105	76-77	0.13164437355699476
1105	78-79	0.14324900195007473
1105	80-81	0.16282575256512644
1105	82-83	0.23982488695669701
1105	84-85	0.24340160701058267
1105	86-87	0.20874329452934148
1105	88-89	0.1822343668647548
1105	90-91	0.16508191189733168
1105	92-93	0.31435390189155044
1105	94-95	0.31658250234980656
1105	96-97	0.2259583426553995
1105	98-99	0.17683134206217588
1105	100	0.07400899320161258
1106	1	0.3339911636946198
1106	2	0.23658953328670407
1106	3	0.27693174336733506
1106	4	0.2859125182443307
1106	5	0.28875597295142796
1106	6	0.2877652517230942
1106	7	0.17211712928065026
1106	8	0.2687955076127082
1106	9	0.4257271397815856
1106	10-11	0.3586405907871608
1106	12-13	0.45120453834343976
1106	14-15	0.43716227048439293
1106	16-17	0.4396261671510757
1106	18-19	0.40534780165782536
1106	20-21	0.3402972043181194
1106	22-23	0.34612094485323297
1106	24-25	0.44501023904137327
1106	26-27	0.3738685137785893
1106	28-29	0.4484158369293141
1106	30-31	0.46910047736794525
1106	32-33	0.3904701833771398
1106	34-35	0.33912680451516053
1106	36-37	0.36115276325346457
1106	38-39	0.30541585246666614
1106	40-41	0.3975970572713976
1106	42-43	0.40332030450523604
1106	44-45	0.3478451639950606
1106	46-47	0.41536732461673864
1106	48-49	0.32396584591823796
1106	50-51	0.3403192063610305
1106	52-53	0.3592466258784981
1106	54-55	0.46339710510619625
1106	56-57	0.33342795537022596
1106	58-59	0.28421780548606534
1106	60-61	0.3289557726191745
1106	62-63	0.2823070224303379
1106	64-65	0.35179556582941274
1106	66-67	0.3095151237102698
1106	68-69	0.3519125281350881
1106	70-71	0.30532777676958744
1106	72-73	0.3150046685767265
1106	74-75	0.25943266378487095
1106	76-77	0.27433603210536717
1106	78-79	0.27882258457204045
1106	80-81	0.29030580993675414
1106	82-83	0.3474961903976208
1106	84-85	0.29191004741015547
1106	86-87	0.18906994346782824
1106	88-89	0.2245018519212536
1106	90-91	0.3288145100812656
1106	92-93	0.3398635281145772
1106	94-95	0.33243815873705884
1106	96-97	0.30014457533059513
1106	98-99	0.34685502012454705
1106	100	0.19910572493298062
1107	1	0.22923258975029626
1107	2	0.21778288017012315
1107	3	0.23167924272194895
1107	4	0.20964457619700738
1107	5	0.25603722632228454
1107	6	0.22906560853395064
1107	7	0.13934361569965859
1107	8	0.14806778435443846
1107	9	0.25013186922223696
1107	10-11	0.29414523302489215
1107	12-13	0.25124865490327153
1107	14-15	0.24489750779876118
1107	16-17	0.16028176165201558
1107	18-19	0.2352260826754602
1107	20-21	0.2633874738896935
1107	22-23	0.26414129452545154
1107	24-25	0.2859364080393121
1107	26-27	0.2936788640810377
1107	28-29	0.28303683750257846
1107	30-31	0.2449706659056332
1107	32-33	0.24938544019249065
1107	34-35	0.24522113911260845
1107	36-37	0.246924013739779
1107	38-39	0.3032467251503945
1107	40-41	0.239231674287538
1107	42-43	0.1770420453640753
1107	44-45	0.18426366440773734
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2216	72-73	-0.25698579223648466
2216	74-75	-0.2680096417565352
2216	76-77	-0.2287160233159824
2216	78-79	-0.31660471752631736
2216	80-81	-0.24115607617486745
2216	82-83	-0.2831041863135795
2216	84-85	-0.18448776242620113
2216	86-87	-0.1941818944411935
2216	88-89	-0.24605030626558744
2216	90-91	-0.2605510619815625
2216	92-93	-0.17426613739888808
2216	94-95	-0.23517063645943992
2216	96-97	-0.20010180692580448
2216	98-99	-0.195656402777594
2216	100	-0.2242060770383958
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	61.0
1	30.0
2	31.0
3	37.0
4	20.0
5	37.0
6	87.0
7	378.0
8	1296.0
9	2065.0
10	2842.0
11	4608.0
12	6882.0
13	8272.0
14	9295.0
15	9107.0
16	9801.0
17	10255.0
18	11218.0
19	12101.0
20	12938.0
21	14161.0
22	15680.0
23	17588.0
24	20127.0
25	24133.0
26	26463.0
27	26013.0
28	27912.0
29	33864.0
30	38755.0
31	43981.0
32	55427.0
33	74759.0
34	110142.0
35	170313.0
36	314008.0
37	592623.0
38	607557.0
39	140957.0
40	142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.75138237695501	19.63460106222611	13.364892106965746	24.249124453853135
2	15.05001537211722	20.794911523794205	39.62658989430906	24.528483209779516
3	18.13681714508403	26.017703295311605	30.362979857889865	25.482499701714502
4	12.173556825229289	17.313740125721317	38.951334195799326	31.561368853250066
5	13.02706737462877	38.716131168182265	35.09144284267498	13.165358614513984
6	30.314521189672522	37.923224366484355	18.13058674488466	13.631667698958463
7	26.589945364269425	33.14285342004689	22.688568192053264	17.57863302363042
8	25.96332737066833	34.78162435047603	20.703043402310495	18.552004876545144
9	26.382152024914003	15.710470851141459	20.204877580006254	37.70249954393829
10-11	24.438577645882265	26.33377256276529	28.76779042078071	20.45985937057174
12-13	25.18074813266122	24.356975195193325	28.088578444516514	22.37369822762894
14-15	22.582710114159514	25.713591128994	25.59093923376364	26.112759523082847
16-17	22.440624324923583	28.49423625989798	26.325969702417662	22.739169712760773
18-19	22.38766799841762	28.28607663645439	27.33473288325404	21.991522481873954
20-21	23.504194239901036	27.344884586888597	26.67243801786662	22.47848315534375
22-23	23.387155881119423	27.11090797301204	26.535316847930098	22.966619297938443
24-25	23.076207370501344	27.444653850781663	26.66794794299162	22.811190835725377
26-27	22.90447981927294	27.450732086301404	26.63270840577916	23.012079688646494
28-29	22.792915079206242	27.775801758709	26.60410275851131	22.827180403573447
30-31	23.035345837525853	27.391690484790267	26.56325142124871	23.00971225643517
32-33	22.872591249703756	27.692759069182575	26.26505965222618	23.169590028887495
34-35	23.29335021376771	27.55422785247239	26.47036306914965	22.682058864610255
36-37	23.369847298633765	27.232127131070115	26.516114857621194	22.881910712674927
38-39	23.141505390356716	27.38014104826123	26.54274971823945	22.935603843142605
40-41	23.071508346899527	27.14283241755165	26.773322907980994	23.01233632756783
42-43	22.98371709340689	27.253006336653502	26.451730616271014	23.311545953668592
44-45	23.006958957970728	27.400695916160977	26.58958113275862	23.002763993109667
46-47	23.158350899322294	27.372660919969753	26.268849539897012	23.200138640810945
48-49	23.126122733836656	27.307799065950615	26.33277581450324	23.23330238570949
50-51	23.021721625462334	27.325561267235337	26.422540213579083	23.230176893723247
52-53	23.03962796623251	27.356745372487897	26.4587145725037	23.1449120887759
54-55	22.779837542755256	27.347193554661047	26.512729076018054	23.36023982656564
56-57	23.211685373120954	27.244397737710464	26.293413332192824	23.250503556975765
58-59	23.03094189792153	27.089450273237176	26.523200771943646	23.35640705689765
60-61	22.849575472946963	27.29565371463717	26.593519165324214	23.261251647091647
62-63	22.937662182147427	27.246132371994474	26.555838720227825	23.26036672563027
64-65	22.563951963322424	27.338415493474056	26.513365516364956	23.584267026838564
66-67	22.880817960958918	27.16719472131362	26.454611162444568	23.497376155282893
68-69	22.92035131482367	27.090035625167513	26.522094039688703	23.46751902032011
70-71	22.93628112686016	26.925309384717277	26.462708866761115	23.675700621661452
72-73	22.928595809088105	26.950962111047843	26.642199450780858	23.478242629083198
74-75	22.79988366845183	27.408625153357242	26.489194526225923	23.30229665196501
76-77	23.029142656740504	26.970836977166755	26.416247905856515	23.583772460236222
78-79	22.929518352257936	27.031888405042093	26.60100859723033	23.43758464546964
80-81	23.027016568396828	27.091172007861587	26.54112567337753	23.340685750364056
82-83	23.093897380052734	26.91896376822072	26.86988163466722	23.11725721705932
84-85	23.04088734347434	27.215624635332645	26.537050610176937	23.206437411016076
86-87	22.924766438408472	27.297141257032305	26.537565868187258	23.240526436371965
88-89	23.033363269246042	26.923993328568024	26.384963618702002	23.65767978348393
90-91	23.001320464842724	26.939234179267153	26.574741894333485	23.484703461556634
92-93	23.16944148523988	26.98882815702776	26.652819557944735	23.188910799787628
94-95	22.733241848770234	26.895422879857474	26.908416457608553	23.462918813763743
96-97	22.763122791654364	26.78204201329844	26.893928131395928	23.560907063651268
98-99	22.71951378952629	27.145733215544105	26.500539867295693	23.634213127633917
100	22.782166918774145	26.742372221421235	26.53478065020107	23.940680209603542
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1491.0
1	1164.0
2	904.0
3	923.5
4	832.0
5	701.5
6	585.5
7	577.5
8	609.0
9	613.0
10	533.5
11	420.0
12	405.5
13	455.5
14	483.0
15	537.5
16	620.5
17	691.5
18	815.5
19	988.5
20	1282.5
21	1698.0
22	2420.5
23	3426.0
24	4655.5
25	6434.5
26	8991.5
27	12161.5
28	15946.0
29	20419.0
30	25769.5
31	31770.0
32	38467.0
33	43333.0
34	46080.0
35	49675.0
36	54975.0
37	61799.0
38	66282.0
39	67921.0
40	69352.0
41	71253.5
42	74193.0
43	78228.0
44	82458.0
45	88655.5
46	93963.5
47	98639.0
48	106267.0
49	104608.0
50	97020.5
51	95759.0
52	93636.0
53	90724.5
54	88934.0
55	83258.0
56	76354.0
57	69300.0
58	62018.5
59	54673.0
60	44866.5
61	36223.0
62	30314.0
63	23587.5
64	16826.0
65	11979.0
66	8889.5
67	6785.0
68	4940.0
69	3530.5
70	2662.5
71	2122.5
72	1666.0
73	1292.0
74	973.5
75	750.0
76	574.5
77	405.0
78	286.5
79	202.0
80	144.0
81	102.5
82	85.5
83	71.5
84	53.0
85	39.5
86	30.5
87	21.5
88	19.5
89	21.0
90	17.5
91	16.0
92	13.0
93	9.0
94	5.5
95	4.0
96	6.5
97	4.5
98	0.5
99	4.0
100	8.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06099432972606298
2	0.008998495907516635
3	0.01079005165380954
4	0.008835627203308188
5	0.011360092118539099
6	0.01079005165380954
7	0.002605899267335134
8	0.0052525157107223795
9	0.006433313816233612
10-11	0.00610757640781672
12-13	0.0053543086508526586
14-15	0.009588894960272251
16-17	0.009181723199751137
18-19	0.007186581573197675
20-21	0.006412955228207557
22-23	0.0022801618589182423
24-25	0.0033184498482470848
26-27	0.004397455013628039
28-29	0.005313591474800547
30-31	0.008265586738578629
32-33	0.009446384844089861
34-35	0.010708617301705317
36-37	0.017162289705964985
38-39	0.017243724058069208
40-41	0.01683655229754809
42-43	0.023168073173651427
44-45	0.02612006843742951
46-47	0.028522381824504086
48-49	0.030232503218692766
50-51	0.03106720532776105
52-53	0.028705609116738588
54-55	0.034955695640737694
56-57	0.037907690904515776
58-59	0.035199998697050365
60-61	0.0358718321019102
62-63	0.031718680144594835
64-65	0.03428386223587786
66-67	0.03605505939414471
68-69	0.03908848901002701
70-71	0.03794840808056789
72-73	0.034365296587982076
74-75	0.03721549891162988
76-77	0.036849044327160886
78-79	0.03554609469349331
80-81	0.04035072146764247
82-83	0.036543665506770046
84-85	0.03328629142260112
86-87	0.03169832155656878
88-89	0.02935708393357237
90-91	0.03135222556012583
92-93	0.03340844295075746
94-95	0.036930478679265105
96-97	0.03220728625722017
98-99	0.029845690046197706
100	0.027036204898602017
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2455966.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.76090006203033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.94649345899506	44.322945989375484
2	14.520778347131255	17.645911239458727
3	5.553791474326951	10.12360106210808
4	2.655273395530239	6.4534720569272315
5	1.4365479987497303	4.364297469317101
6	0.8467334239366936	3.086897097059892
7	0.543878206022802	2.3132570525447473
8	0.37809850043064064	1.8378884158615751
9	0.2506599152733589	1.3707289855333396
>10	0.8535240639004719	7.841303998743293
>50	0.012641060836609059	0.5066149988789211
>100	0.001580154866174577	0.13308163419155383
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	8.143435210422294E-5	0.0
10-11	0.0	0.0	0.0	1.4251011618239014E-4	8.143435210422294E-5
12-13	0.0	4.071717605211147E-5	0.0	1.6286870420844588E-4	3.2573740841689177E-4
14-15	0.0	4.071717605211147E-5	0.0	1.6286870420844588E-4	3.6645458446900325E-4
16-17	0.0	4.071717605211147E-5	0.0	1.6286870420844588E-4	4.071717605211147E-4
18-19	0.0	4.071717605211147E-5	0.0	2.2394446828661306E-4	4.071717605211147E-4
20-21	0.0	4.071717605211147E-5	0.0	4.275303485471704E-4	4.886061126253376E-4
22-23	0.0	4.071717605211147E-5	0.0	7.329091689380065E-4	5.700404647295606E-4
24-25	0.0	4.071717605211147E-5	0.0	0.0011400809294591211	5.700404647295606E-4
26-27	4.071717605211147E-5	4.071717605211147E-5	0.0	0.002076575978657685	6.10757640781672E-4
28-29	4.071717605211147E-5	4.071717605211147E-5	0.0	0.005171081358618157	6.10757640781672E-4
30-31	4.071717605211147E-5	4.071717605211147E-5	0.0	0.012968420572597502	6.10757640781672E-4
32-33	4.071717605211147E-5	4.071717605211147E-5	0.0	0.029825331458171653	6.10757640781672E-4
34-35	4.071717605211147E-5	4.071717605211147E-5	0.0	0.058754885043196856	6.92191992885895E-4
36-37	4.071717605211147E-5	4.071717605211147E-5	0.0	0.10181329871830473	6.92191992885895E-4
38-39	4.071717605211147E-5	4.071717605211147E-5	0.0	0.16150467881070013	6.92191992885895E-4
40-41	4.071717605211147E-5	4.071717605211147E-5	0.0	0.22695753931446933	6.92191992885895E-4
42-43	4.071717605211147E-5	4.071717605211147E-5	0.0	0.28825724786092316	6.92191992885895E-4
44-45	4.071717605211147E-5	4.071717605211147E-5	0.0	0.3631361346207561	7.532677569640621E-4
46-47	4.071717605211147E-5	4.071717605211147E-5	0.0	0.45102415912923877	8.347021090682851E-4
48-49	4.071717605211147E-5	4.071717605211147E-5	0.0	0.5449383256934339	9.364950491985638E-4
50-51	4.071717605211147E-5	4.071717605211147E-5	0.0	0.6444511039647942	9.772122252506752E-4
52-53	4.071717605211147E-5	4.071717605211147E-5	0.0	0.7330313204661628	9.772122252506752E-4
54-55	4.071717605211147E-5	4.071717605211147E-5	0.0	0.8285538154844163	9.772122252506752E-4
56-57	4.071717605211147E-5	4.071717605211147E-5	0.0	0.9435391206555791	9.772122252506752E-4
58-59	4.071717605211147E-5	4.071717605211147E-5	0.0	1.0677875833785972	9.772122252506752E-4
60-61	4.071717605211147E-5	4.071717605211147E-5	0.0	1.19810290533338	9.772122252506752E-4
62-63	4.071717605211147E-5	4.071717605211147E-5	0.0	1.3246926056793946	0.0010179294013027867
64-65	4.071717605211147E-5	4.071717605211147E-5	0.0	1.4507936999127837	0.0010586465773548982
66-67	4.071717605211147E-5	4.071717605211147E-5	0.0	1.5930391544508353	0.0010586465773548982
68-69	4.071717605211147E-5	4.071717605211147E-5	0.0	1.7530576563356335	0.0010586465773548982
70-71	4.071717605211147E-5	4.071717605211147E-5	0.0	1.92278720470886	0.0010993637534070096
72-73	4.071717605211147E-5	4.071717605211147E-5	0.0	2.0910916519202627	0.0011807981055112326
74-75	4.071717605211147E-5	4.071717605211147E-5	0.0	2.2597218365400824	0.001221515281563344
76-77	4.071717605211147E-5	4.071717605211147E-5	0.0	2.437024779659002	0.001221515281563344
78-79	4.071717605211147E-5	4.071717605211147E-5	0.0	2.638289780884589	0.0012418738695893997
80-81	4.071717605211147E-5	4.071717605211147E-5	0.0	2.8481257476691453	0.0012622324576154556
82-83	4.071717605211147E-5	4.071717605211147E-5	0.0	3.066736265892932	0.0013436668097196785
84-85	4.071717605211147E-5	4.071717605211147E-5	0.0	3.2870161883348548	0.00138438398577179
86-87	4.071717605211147E-5	4.071717605211147E-5	0.0	3.5118157173185622	0.0014047425737978457
88	4.071717605211147E-5	4.071717605211147E-5	0.0	3.69284428204625	0.0014251011618239015
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	8805	0.0	28.835634	1
TACATGG	9260	0.0	26.644646	2
CATGGGG	6340	0.0	24.981075	4
ACATGGG	9435	0.0	24.905184	3
ATGGGGG	3895	0.0	21.598124	5
GTATCAA	8065	0.0	19.821644	1
GCATCGT	815	0.0	19.028313	8
GTACTAG	970	0.0	18.904186	1
GGTATCA	6300	0.0	18.80724	1
CGCCGTA	315	7.763447E-9	17.906187	94
CAACGCA	8670	0.0	17.725527	5
ATCAACG	8825	0.0	17.573605	3
TCAACGC	8995	0.0	17.346323	4
TATCAAC	9230	0.0	17.158915	2
GGGCTAT	1380	0.0	17.026873	6
CTAGTAC	1275	0.0	16.955448	3
AACGCAG	9090	0.0	16.90549	6
GTAAGGT	1095	0.0	16.73867	4
GCTATTG	1350	0.0	16.709036	8
GAGTACA	8110	0.0	16.638958	1
>>END_MODULE