FastQCFastQC Report
Mon 16 Jan 2017
ERR1146496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2455966
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90570.36877546350397356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82640.3364867428946492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47150.1919814850857056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93000.032.1255841
GTATCAA136800.029.7158341
GTACATG80600.025.91841
CAACGCA162250.024.8055275
ATCAACG163300.024.5596543
TATCAAC165350.024.4397132
TCAACGC164700.024.4365314
TACATGG83200.024.1441382
AACGCAG168600.024.0576636
GTACTAG8650.023.9329831
ACATGGG81750.023.7531783
GAGTACT106100.022.52373512-13
CATGGGG60750.022.2123744
ACGCAGA182800.021.9813697
CGCAGAG185250.021.665298
GTACTTT113700.020.87352414-15
ATGGGGG36550.020.8394875
AGTACTT110500.020.60612712-13
GCAGAGT196450.020.2148029
AGAGTAC159050.020.13710410-11
Produced by FastQC (version 0.11.5)