FastQCFastQC Report
Mon 16 Jan 2017
ERR1146495_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146495_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746859
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25750.032.325911
GTATCAA36350.030.920621
GTACTAG9550.030.531141
CAACGCA39100.027.4000975
ATCAACG40900.026.080833
TACATGG55500.025.5701242
GCATCGT9050.025.4428868
TATCAAC44100.024.934242
GTACATG60150.024.6280291
AACGCAG44800.024.543246
ACATGGG57500.023.8634873
TAACGCC2653.6379788E-1123.0510924
TAGGCAT13800.022.8133855
GTCCTAC9100.022.221931
TCAACGC48250.022.2040184
ACGCAGA48850.021.8351087
TAGTACT13400.021.741074
CGCAGAG48450.021.7256688
CTGTGCG12150.021.6586139
CTAGTAC13700.021.2662073
Produced by FastQC (version 0.11.5)