##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146495_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1746859
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.502261487618636	31.0	31.0	34.0	28.0	34.0
2	31.523921507116487	33.0	31.0	34.0	28.0	34.0
3	31.68031249230762	33.0	31.0	34.0	28.0	34.0
4	35.18347903293855	37.0	35.0	37.0	32.0	37.0
5	35.17255714399388	37.0	35.0	37.0	32.0	37.0
6	35.29986965175781	37.0	35.0	37.0	33.0	37.0
7	35.182276875237214	37.0	35.0	37.0	32.0	37.0
8	35.166794228956086	37.0	35.0	37.0	32.0	37.0
9	36.77296679354201	39.0	37.0	39.0	33.0	39.0
10-11	36.757812164576535	39.0	37.5	39.0	33.0	39.0
12-13	36.716594470418045	39.0	38.0	39.0	33.0	39.0
14-15	38.141166802815796	40.5	39.0	41.0	33.0	41.0
16-17	38.16478404954263	40.5	38.5	41.0	33.0	41.0
18-19	38.208003050045825	40.0	38.5	41.0	33.0	41.0
20-21	38.323130830822635	41.0	39.0	41.0	33.0	41.0
22-23	38.352980406546834	40.5	39.0	41.0	33.0	41.0
24-25	38.41957650846462	41.0	39.0	41.0	33.0	41.0
26-27	38.46202641426698	41.0	39.0	41.0	33.0	41.0
28-29	38.438910066582366	40.0	39.0	41.0	33.0	41.0
30-31	38.423433717317764	40.0	38.0	41.0	33.0	41.0
32-33	38.439294470818766	40.0	38.0	41.0	33.0	41.0
34-35	38.37407684306518	40.0	38.0	41.0	33.0	41.0
36-37	38.277665799014116	40.0	38.0	41.0	33.0	41.0
38-39	38.23554591412358	40.0	38.0	41.0	33.0	41.0
40-41	38.17074331700498	40.0	38.0	41.0	33.0	41.0
42-43	38.039972316025505	40.0	38.0	41.0	32.5	41.0
44-45	37.88534878888336	40.0	38.0	41.0	32.0	41.0
46-47	37.78000628556741	40.0	37.5	41.0	32.0	41.0
48-49	37.66679022176375	40.0	37.0	41.0	32.0	41.0
50-51	37.536528134211174	40.0	37.0	41.0	31.0	41.0
52-53	37.41793584943032	40.0	36.0	41.0	31.0	41.0
54-55	37.246820722222	40.0	36.0	41.0	31.0	41.0
56-57	37.122078542114735	39.5	35.5	41.0	31.0	41.0
58-59	36.90905934594606	39.0	35.0	41.0	31.0	41.0
60-61	36.73603421913274	39.0	35.0	41.0	31.0	41.0
62-63	36.502266353495045	38.5	35.0	41.0	30.5	41.0
64-65	36.260218197347356	38.0	35.0	40.0	30.0	41.0
66-67	36.0073586362723	37.0	35.0	40.0	30.0	41.0
68-69	35.71976788052156	37.0	35.0	40.0	30.0	41.0
70-71	35.46942025658625	36.0	35.0	39.0	30.0	41.0
72-73	35.15515362144283	36.0	35.0	39.0	29.5	41.0
74-75	34.83482124201209	35.0	34.0	39.0	29.0	40.5
76-77	34.52051253134913	35.0	34.0	37.5	29.0	39.5
78-79	34.226096095906996	35.0	34.0	37.0	29.0	39.0
80-81	33.94288548760947	35.0	34.0	36.5	29.0	39.0
82-83	33.67227005728567	35.0	34.0	36.0	28.5	38.0
84-85	33.43802819804002	35.0	34.0	36.0	28.0	37.0
86-87	33.22836617036636	35.0	34.0	35.5	28.0	37.0
88-89	33.07650960953346	35.0	34.0	35.0	28.0	36.5
90-91	32.89712220619981	35.0	34.0	35.0	27.5	36.0
92-93	32.791801169985675	35.0	34.0	35.0	27.0	36.0
94-95	32.689133467555195	35.0	33.5	35.0	27.0	36.0
96-97	32.599843776744436	35.0	33.0	35.0	27.0	35.5
98-99	32.52166688897043	35.0	33.0	35.0	27.0	35.0
100	31.613824584582957	34.0	32.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4327093180306214
1101	2	0.44483366551888537
1101	3	0.33695816025581493
1101	4	0.4742858144902087
1101	5	0.32619482707139014
1101	6	0.367228264762808
1101	7	0.456283720129008
1101	8	0.5070438590819677
1101	9	0.4871753970118675
1101	10-11	0.48423665115503667
1101	12-13	0.38547876067697473
1101	14-15	0.5485722777655511
1101	16-17	0.4920885116543232
1101	18-19	0.4699461830665612
1101	20-21	0.43569424142975066
1101	22-23	0.4762969102507455
1101	24-25	0.4710032597214493
1101	26-27	0.5143662578958157
1101	28-29	0.5534847013087898
1101	30-31	0.5261007836815708
1101	32-33	0.5316414902580391
1101	34-35	0.4820415244789089
1101	36-37	0.4792611729083518
1101	38-39	0.49563950410728097
1101	40-41	0.5152533121447362
1101	42-43	0.5042747984368816
1101	44-45	0.33632767998466306
1101	46-47	0.3766070155538017
1101	48-49	0.34257773483208354
1101	50-51	0.36637121644855597
1101	52-53	0.31632082713355913
1101	54-55	0.456023423567089
1101	56-57	0.32704151420947625
1101	58-59	0.4467025320667517
1101	60-61	0.4269284174188783
1101	62-63	0.38966296771897646
1101	64-65	0.3491815614958753
1101	66-67	0.34542436918620467
1101	68-69	0.2822936027296308
1101	70-71	0.24379346920738243
1101	72-73	0.1502121566816612
1101	74-75	0.16032538740788738
1101	76-77	0.25397695915668095
1101	78-79	0.27714739328454385
1101	80-81	0.19416328821186823
1101	82-83	0.21395523730380717
1101	84-85	0.2641179171924435
1101	86-87	0.2545012733686107
1101	88-89	0.19985261808374588
1101	90-91	0.23330602816816537
1101	92-93	0.2386397149487749
1101	94-95	0.07710936389742784
1101	96-97	0.1285348845199863
1101	98-99	0.22069594538935888
1101	100	0.10846105467717138
1102	1	0.2810993001415305
1102	2	0.2865152397585966
1102	3	0.32264689013059566
1102	4	0.4532661364937809
1102	5	0.40580126714294806
1102	6	0.4097148479470647
1102	7	0.29349302245459086
1102	8	0.3174195299227591
1102	9	0.47957253475784256
1102	10-11	0.4726087441782951
1102	12-13	0.40359146192921
1102	14-15	0.464046338588453
1102	16-17	0.4236627513680986
1102	18-19	0.354561567681948
1102	20-21	0.45157080672492356
1102	22-23	0.49016095318454944
1102	24-25	0.45781005757476123
1102	26-27	0.48238951370977645
1102	28-29	0.5282163649939378
1102	30-31	0.5258771700858631
1102	32-33	0.49004936145660594
1102	34-35	0.5426408089154009
1102	36-37	0.5678121568081735
1102	38-39	0.4983228672557587
1102	40-41	0.42312451071360613
1102	42-43	0.44456996838321317
1102	44-45	0.39804503239969335
1102	46-47	0.3669916309384149
1102	48-49	0.47428614270328495
1102	50-51	0.45581665473119415
1102	52-53	0.4115802189045823
1102	54-55	0.3782058922611853
1102	56-57	0.29081611170500565
1102	58-59	0.3778295445891118
1102	60-61	0.34709836375161274
1102	62-63	0.3046898013504631
1102	64-65	0.3078130462901498
1102	66-67	0.28907374306813693
1102	68-69	0.2816227619425149
1102	70-71	0.27040348709648043
1102	72-73	0.2562050010466024
1102	74-75	0.24082628186226884
1102	76-77	0.14082847972913015
1102	78-79	0.15773773317720696
1102	80-81	0.18857294831920512
1102	82-83	0.17549369884227417
1102	84-85	0.06577265780067165
1102	86-87	0.21268553097146992
1102	88-89	0.24569340520360328
1102	90-91	0.2257031659141404
1102	92-93	0.1798293392779371
1102	94-95	0.21552617963982357
1102	96-97	0.17929517074539092
1102	98-99	0.13192134789382948
1102	100	0.04227143034801273
1103	1	0.24663605930595267
1103	2	0.2816114742393836
1103	3	0.264360246214693
1103	4	0.1555532492705609
1103	5	0.1254728127947331
1103	6	0.1974974821584894
1103	7	0.26564772136881487
1103	8	0.30825196250827247
1103	9	0.2235642714783026
1103	10-11	0.252463420908164
1103	12-13	0.23866984665630753
1103	14-15	0.32305078804666465
1103	16-17	0.23781723630555263
1103	18-19	0.29365960837844796
1103	20-21	0.2610996172815305
1103	22-23	0.27027562742851075
1103	24-25	0.25866238528018926
1103	26-27	0.2604719986094608
1103	28-29	0.33278752249815113
1103	30-31	0.2593091136715202
1103	32-33	0.29644797501297404
1103	34-35	0.3174039004392455
1103	36-37	0.31539193789105724
1103	38-39	0.28410674599386
1103	40-41	0.36924995774413105
1103	42-43	0.30597280640751734
1103	44-45	0.31481396737750345
1103	46-47	0.29239397807482703
1103	48-49	0.23453165745115712
1103	50-51	0.3263459561757003
1103	52-53	0.16060689992027477
1103	54-55	0.27669475996217585
1103	56-57	0.2749094559259788
1103	58-59	0.34327662231145695
1103	60-61	0.22846790950724483
1103	62-63	0.14682214283851636
1103	64-65	0.17397116411268598
1103	66-67	0.16814225182308462
1103	68-69	0.15421630991342994
1103	70-71	0.10349825992708617
1103	72-73	0.1864253656567314
1103	74-75	0.0739767122545274
1103	76-77	0.16684416139462144
1103	78-79	0.20801578498161177
1103	80-81	0.18359523494424224
1103	82-83	0.2440120906180141
1103	84-85	0.27652562004163883
1103	86-87	0.1892240003767256
1103	88-89	0.1866476157473116
1103	90-91	0.25483252431422443
1103	92-93	0.173364408655182
1103	94-95	0.24237711806957662
1103	96-97	0.2902639584568263
1103	98-99	0.23377645572396233
1103	100	0.25298859868007995
1104	1	0.07908624029204248
1104	2	0.10993055794786244
1104	3	0.07957474143098153
1104	4	0.08549025495764795
1104	5	0.1610236130080338
1104	6	0.11942251371396395
1104	7	0.05091795760386475
1104	8	0.15049554853689528
1104	9	0.09609254747962837
1104	10-11	0.08522186689535971
1104	12-13	0.14032319678520366
1104	14-15	0.16095205649836686
1104	16-17	0.10843004124915723
1104	18-19	0.0599357817445636
1104	20-21	0.11615875349802707
1104	22-23	0.11035525328975382
1104	24-25	0.14710772369009817
1104	26-27	0.11265587799920951
1104	28-29	0.14913247458814283
1104	30-31	0.1466145185420089
1104	32-33	0.12604331045232442
1104	34-35	0.12729296513243327
1104	36-37	0.24736988933480575
1104	38-39	0.2455902961423746
1104	40-41	0.1416568048538025
1104	42-43	0.21651709141313802
1104	44-45	0.13758810818106326
1104	46-47	0.10276598823234906
1104	48-49	0.10708435409106443
1104	50-51	0.12036725534638038
1104	52-53	0.15213293833503627
1104	54-55	0.14349557612178643
1104	56-57	0.011431450355892991
1104	58-59	0.036591490726131326
1104	60-61	0.09476974276822148
1104	62-63	0.13159323215572272
1104	64-65	0.18053001912735311
1104	66-67	0.07610797253627766
1104	68-69	0.04359364194773008
1104	70-71	0.06673672454687107
1104	72-73	0.08034110935082595
1104	74-75	0.030129637938145493
1104	76-77	0.045154388065533624
1104	78-79	0.01925882828888348
1104	80-81	0.044446618592104414
1104	82-83	0.04981498107395055
1104	84-85	0.03304037211201205
1104	86-87	-0.04273160800516962
1104	88-89	-0.014005290071764875
1104	90-91	0.10158390508264148
1104	92-93	0.09745075784625357
1104	94-95	0.05959939071252052
1104	96-97	0.022242529528725186
1104	98-99	-0.04160715788175651
1104	100	-0.04544162809288821
1105	1	0.1904226780808287
1105	2	0.20796701469264178
1105	3	0.22961135678373168
1105	4	0.2277728143883735
1105	5	0.1621648207809656
1105	6	0.19779325516105217
1105	7	0.0879263168127764
1105	8	0.1383601983838716
1105	9	0.20890096092978183
1105	10-11	0.3676630108737484
1105	12-13	0.2499447277168585
1105	14-15	0.3550450472808748
1105	16-17	0.2694103556242098
1105	18-19	0.22538531069729828
1105	20-21	0.2761178199261636
1105	22-23	0.30233681728815753
1105	24-25	0.2709892488897765
1105	26-27	0.2117486337139951
1105	28-29	0.25561739905108993
1105	30-31	0.29968718826383167
1105	32-33	0.2791496927333341
1105	34-35	0.2570769487723865
1105	36-37	0.29437163820518464
1105	38-39	0.24194080704240406
1105	40-41	0.16840650758933862
1105	42-43	0.24247961007424834
1105	44-45	0.2113436588326394
1105	46-47	0.19423861806795628
1105	48-49	0.20999030977016986
1105	50-51	0.2726754948798913
1105	52-53	0.19224218375762092
1105	54-55	0.2844055408348609
1105	56-57	0.1990785663593826
1105	58-59	0.2169900718874871
1105	60-61	0.1757002127480689
1105	62-63	0.25554987864114764
1105	64-65	0.20717239378983265
1105	66-67	0.17014200398448764
1105	68-69	0.1320140721935985
1105	70-71	0.14867581072068248
1105	72-73	0.10618149318212033
1105	74-75	0.09178949042938456
1105	76-77	0.128360915887761
1105	78-79	0.15846635058978364
1105	80-81	0.18524808407622118
1105	82-83	0.16507052478223727
1105	84-85	0.19244104813666496
1105	86-87	0.17492002029471365
1105	88-89	0.19328386714939683
1105	90-91	0.13129119474178452
1105	92-93	0.16007388606725215
1105	94-95	0.14907511608048196
1105	96-97	0.05989318409612565
1105	98-99	0.09418535746163315
1105	100	-0.07660993474031841
1106	1	0.17481155772208368
1106	2	0.22473688597295904
1106	3	0.20428261922594615
1106	4	0.18198885842374324
1106	5	0.10373293724195776
1106	6	0.1807474233761326
1106	7	0.05456215687651422
1106	8	0.1254196331022186
1106	9	0.17978461038001825
1106	10-11	0.2273011438955237
1106	12-13	0.25470646241025463
1106	14-15	0.2773756957943547
1106	16-17	0.21040649123315092
1106	18-19	0.22927904419119471
1106	20-21	0.26444369938900536
1106	22-23	0.27312905678788724
1106	24-25	0.23428587244849552
1106	26-27	0.1945121940890857
1106	28-29	0.2410633930768853
1106	30-31	0.28295350336667724
1106	32-33	0.26097736310747877
1106	34-35	0.21648007483536702
1106	36-37	0.2959454312083807
1106	38-39	0.244968663320158
1106	40-41	0.26652667218389325
1106	42-43	0.20627072842543726
1106	44-45	0.24709069209988854
1106	46-47	0.2519182968335656
1106	48-49	0.3014432905998845
1106	50-51	0.23948156827657385
1106	52-53	0.22091485778343412
1106	54-55	0.2557032883741073
1106	56-57	0.12585864534639057
1106	58-59	0.2878040704167475
1106	60-61	0.2983673316920843
1106	62-63	0.27176893023695214
1106	64-65	0.1475838833126133
1106	66-67	0.11713493127723495
1106	68-69	0.1759047723245999
1106	70-71	0.1375628937866864
1106	72-73	0.09603317935744116
1106	74-75	0.1310583241045009
1106	76-77	0.15431135124142514
1106	78-79	0.16895379375749542
1106	80-81	0.08853627778098883
1106	82-83	0.08532234424932028
1106	84-85	0.13553347665518345
1106	86-87	0.09862307672250381
1106	88-89	0.11083122647168153
1106	90-91	0.1718537702808689
1106	92-93	0.13342890815510344
1106	94-95	-0.010481109849678205
1106	96-97	0.04897968559974686
1106	98-99	0.16663757648931465
1106	100	-0.04916334977516357
1107	1	0.11761574790632423
1107	2	0.1271702769734624
1107	3	0.11463294961876258
1107	4	0.06602068523459792
1107	5	0.028197848751482013
1107	6	0.056124980771222965
1107	7	0.04294847121116163
1107	8	0.05819228289577438
1107	9	0.15354159497825748
1107	10-11	0.06764915683228168
1107	12-13	0.07009553563596427
1107	14-15	0.22438470187596948
1107	16-17	0.21180682804091333
1107	18-19	0.1445223599924219
1107	20-21	0.13831017291362002
1107	22-23	0.08676318514947923
1107	24-25	0.15439421507288387
1107	26-27	0.1166132848777508
1107	28-29	0.13753213056347136
1107	30-31	0.16952862948319591
1107	32-33	0.14631703040652866
1107	34-35	0.1485015706383095
1107	36-37	0.08132166212392633
1107	38-39	0.050867427768146456
1107	40-41	0.1719033255847222
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2216	50-51	-0.359302158801988
2216	52-53	-0.2702593474487216
2216	54-55	-0.21440762529947932
2216	56-57	-0.25535177960030353
2216	58-59	-0.2567203663878601
2216	60-61	-0.386171009581183
2216	62-63	-0.3464838136180788
2216	64-65	-0.26752731102695293
2216	66-67	-0.2808008854745694
2216	68-69	-0.2934646770455629
2216	70-71	-0.27912072019729095
2216	72-73	-0.29337464763452914
2216	74-75	-0.3040545772363501
2216	76-77	-0.254056497872547
2216	78-79	-0.21381513256170592
2216	80-81	-0.22398885562737547
2216	82-83	-0.24732159368760875
2216	84-85	-0.18781915208077749
2216	86-87	-0.18254027915919835
2216	88-89	-0.20958361164846195
2216	90-91	-0.17201117048941228
2216	92-93	-0.1388794360904555
2216	94-95	-0.23068485034499275
2216	96-97	-0.09571443909509014
2216	98-99	-0.1145248591760577
2216	100	-0.024940955574734858
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	46.0
1	28.0
2	18.0
3	30.0
4	23.0
5	28.0
6	84.0
7	466.0
8	1447.0
9	1837.0
10	1822.0
11	1932.0
12	2192.0
13	2537.0
14	3267.0
15	3866.0
16	4214.0
17	4266.0
18	4910.0
19	5286.0
20	6064.0
21	6890.0
22	7772.0
23	9072.0
24	11411.0
25	14009.0
26	15807.0
27	14470.0
28	15108.0
29	18903.0
30	21996.0
31	23587.0
32	29894.0
33	40494.0
34	61351.0
35	96964.0
36	184213.0
37	409892.0
38	532623.0
39	187676.0
40	364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.15377741626904	20.653293389163522	13.182836226994393	24.010092967573048
2	14.98842085677072	21.499878340275096	40.170724376315	23.340976426639187
3	17.796663540668323	26.85616170915391	30.672742324987034	24.674432425190737
4	11.942130544057617	18.267179636913248	40.01694633850311	29.773743480526022
5	12.642960197865616	38.991091467045294	35.6139788393716	12.751969495717491
6	29.03326461055598	39.124463037699435	18.90027143684041	12.942000914904177
7	25.540242545259694	33.56047144276875	23.59097279151458	17.30831322045697
8	24.92762301796987	35.541062666415534	21.290139384012775	18.24117493160182
9	25.87456425646366	15.858001851462763	21.208122391192212	37.059311500881364
10-11	23.86889585617955	27.00400490401083	29.21043765405541	19.91666158575421
12-13	24.450500143838763	25.128216871642866	28.496477023725525	21.924805960792845
14-15	22.178548691454377	26.417810556303646	26.07106543186128	25.332575320380702
16-17	21.62305445410212	28.989964973589927	27.05174725565386	22.33523331665409
18-19	21.335641671690393	29.14878305569725	28.256677836230494	21.25889743638186
20-21	22.801404563773144	28.14944114288691	27.459925936082445	21.5892283572575
22-23	22.868902245291565	28.06786194957554	26.939033472997366	22.124202332135532
24-25	22.46669605390459	28.225573766737845	27.452527207048355	21.855202972309208
26-27	22.35659094018728	28.268191157502798	27.040639031262863	22.334578871047057
28-29	22.0268624742307	28.58473083425266	27.264220560595714	22.12418613092093
30-31	22.486353478192044	28.19821256255759	27.275811026624353	22.039622932626017
32-33	22.421296041844148	28.453739454230675	26.77892586587522	22.34603863804996
34-35	22.666082689957033	28.405571824841342	27.19376289973063	21.734582585471003
36-37	22.55271686868227	28.03809968775112	27.132815561886225	22.27636788168038
38-39	22.49232542433826	28.056124387243496	27.343762301293406	22.107787887124836
40-41	22.449165383878757	27.7455215982168	27.581137707151044	22.224175310753402
42-43	22.263548198507454	27.87957373198665	27.276623570713227	22.58025449879267
44-45	22.30353174874717	28.32393608477594	27.247974268536364	22.124557897940527
46-47	22.30435448612139	28.07443154894413	26.99303242135884	22.62818154357564
48-49	22.502385783735328	27.845224266534345	27.262299921289895	22.390090028440433
50-51	22.478219240367732	27.81643804552113	27.091892071425544	22.613450642685592
52-53	22.21540144219413	28.02967421072297	27.057531720683333	22.69739262639957
54-55	22.18997109138056	27.934843362012884	27.145955313046905	22.729230233559655
56-57	22.685695305072905	27.781105753261482	26.888937885851078	22.64426105581454
58-59	22.18664966972503	27.867596802536255	27.382657106459668	22.56309642127905
60-61	22.29243262435717	28.134241601667643	27.03392548305444	22.539400290920753
62-63	22.496332157103932	27.816851805403324	26.86756481573742	22.81925122175532
64-65	22.108856490589798	27.839474658497387	27.120548912463033	22.931119938449783
66-67	22.57221199671511	27.732596562320676	26.844456865222217	22.850734575741992
68-69	22.222880819638803	27.75252199858545	27.09996649743577	22.924630684339988
70-71	22.398700219884816	27.576974718257368	27.043541431207036	22.98078363065078
72-73	22.60993435793181	27.74596448026801	27.04447482766273	22.599626334137454
74-75	22.62153130735817	27.985516749658533	27.031277292107514	22.36167465087578
76-77	22.555545947208945	27.74368087359342	26.919132018873338	22.7816411603243
78-79	22.53243536550097	27.88344744053397	26.809665753236377	22.774451440728683
80-81	22.453734791476897	27.979730846116336	27.042356930567895	22.524177431838872
82-83	22.818298437714756	27.59306134603931	27.40974481147203	22.178895404773904
84-85	22.949956921371268	27.85871584168253	26.78577308600751	22.4055541509387
86-87	22.345454545454547	27.89726556907659	27.110064423765213	22.647215461703652
88-89	22.866126798490857	27.48271264145341	26.911043723824875	22.740116836230857
90-91	22.743947448442288	27.493161047663932	27.037984889880985	22.724906614012795
92-93	23.00952256602187	27.502340053847725	27.008645242682693	22.479492137447703
94-95	22.395434094079032	27.70228034724205	27.200579328520675	22.70170623015824
96-97	22.485981372634356	27.6457582199092	27.006931432419677	22.861328975036766
98-99	22.349265831164217	27.887220626303293	26.86534258683172	22.898170955700774
100	22.711192195720837	27.666458228828102	26.5675212618048	23.054828313646265
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1266.0
1	883.0
2	516.5
3	555.0
4	513.0
5	430.5
6	382.5
7	380.0
8	426.0
9	441.0
10	356.5
11	273.0
12	264.5
13	271.0
14	287.5
15	318.5
16	381.0
17	460.0
18	566.0
19	732.5
20	1028.0
21	1489.0
22	2060.5
23	2836.5
24	3820.5
25	5188.5
26	7452.0
27	10315.5
28	12897.5
29	16285.5
30	20923.0
31	25790.0
32	30801.5
33	35285.5
34	39646.0
35	43391.0
36	47356.0
37	53119.0
38	55929.0
39	55744.0
40	55842.0
41	55680.5
42	56227.5
43	58475.0
44	61362.5
45	66461.0
46	70787.5
47	72875.0
48	73500.0
49	71247.5
50	68241.0
51	67777.5
52	66643.0
53	63122.0
54	59022.0
55	52221.0
56	46596.5
57	39819.5
58	32927.0
59	30031.0
60	25131.5
61	18969.0
62	14428.0
63	10739.0
64	7531.5
65	4885.0
66	3487.0
67	2529.5
68	1931.0
69	1569.5
70	1249.5
71	971.5
72	770.5
73	665.0
74	558.5
75	476.0
76	382.5
77	278.0
78	211.5
79	165.0
80	135.5
81	103.0
82	80.5
83	70.5
84	52.5
85	38.0
86	32.5
87	24.5
88	18.5
89	13.5
90	10.5
91	7.5
92	7.5
93	9.0
94	6.5
95	4.5
96	4.5
97	3.5
98	5.0
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06234046365505172
2	0.010533191287905893
3	0.012422296247149885
4	0.00967450721552226
5	0.012536787456801035
6	0.013051997900231217
7	0.0029195258461043507
8	0.005839051692208701
9	0.007556419836975966
10-11	0.007184323405609726
12-13	0.006382884938051669
14-15	0.01070492810238262
16-17	0.010132472054126864
18-19	0.008472349514185174
20-21	0.00712707780078415
22-23	0.002747789031627624
24-25	0.00394994673296471
26-27	0.005152104434301795
28-29	0.0062970165308133055
30-31	0.009645884413109472
32-33	0.01070492810238262
34-35	0.012250559432673157
36-37	0.01920590041898058
38-39	0.019778356467236337
40-41	0.019320391628631733
42-43	0.025531539752206674
44-45	0.029452863682758595
46-47	0.03185717908543277
48-49	0.03305933678676985
50-51	0.03394664366156627
52-53	0.032143407109560644
54-55	0.038182818418658865
56-57	0.041159589869588786
58-59	0.03792521319694377
60-61	0.03886976567656577
62-63	0.03440460850017088
64-65	0.03678030110043226
66-67	0.038984256886216914
68-69	0.041445817893716666
70-71	0.04107372146235042
72-73	0.037009283519734565
74-75	0.040300905797205154
76-77	0.03961395853929825
78-79	0.0397284497489494
80-81	0.043363545655373446
82-83	0.04001467777307728
84-85	0.036265090657002085
86-87	0.03486257333877548
88-89	0.03254412634333967
90-91	0.03503431015325221
92-93	0.03655131868112996
94-95	0.04004330057549006
96-97	0.03497706454842663
98-99	0.03231514392403737
100	0.030626398581682895
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1746859.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.551873272619495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.59932537044558	41.33722752222761
2	15.367949150319221	17.99644422219103
3	6.139704477664281	10.784735955226003
4	2.9542515863142245	6.919078579892224
5	1.6695784762362855	4.8878473679640075
6	1.0004560993006315	3.5147147244641888
7	0.611586239568826	2.5066663996158263
8	0.44017668840230906	2.0618535741514656
9	0.28192858017132605	1.4856701848308924
>10	0.9219362322286113	7.835645197626248
>50	0.009763054606401741	0.38118377180867324
>100	0.0033440447422681896	0.28893250000183907
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	2.8622802412787753E-5	0.0
20-21	0.0	0.0	0.0	8.586840723836326E-5	0.0
22-23	0.0	0.0	0.0	6.583244554941183E-4	0.0
24-25	0.0	0.0	0.0	0.0020322189713079305	0.0
26-27	0.0	0.0	0.0	0.003119885462993865	0.0
28-29	0.0	0.0	0.0	0.01018971765895244	0.0
30-31	0.0	0.0	0.0	0.02879453922726448	0.0
32-33	0.0	0.0	0.0	0.06279842849365633	0.0
34-35	0.0	0.0	0.0	0.11652342862245894	0.0
36-37	0.0	0.0	0.0	0.197697696265125	0.0
38-39	0.0	0.0	0.0	0.3066933278530208	0.0
40-41	0.0	0.0	0.0	0.4199537570004219	0.0
42-43	0.0	0.0	0.0	0.5304091515113698	0.0
44-45	0.0	0.0	0.0	0.6600990692437111	0.0
46-47	0.0	0.0	0.0	0.8161792108006427	0.0
48-49	0.0	0.0	0.0	0.9800447546138527	0.0
50-51	0.0	0.0	0.0	1.1493200080830794	0.0
52-53	0.0	0.0	0.0	1.3118116573804754	0.0
54-55	0.0	0.0	0.0	1.47788115697947	0.0
56-57	0.0	0.0	0.0	1.6728310642129673	0.0
58-59	0.0	0.0	0.0	1.8840673460193411	0.0
60-61	0.0	0.0	0.0	2.1062089155449866	0.0
62-63	0.0	0.0	0.0	2.326089283680022	0.0
64-65	0.0	0.0	0.0	2.547343546330872	0.0
66-67	0.0	0.0	0.0	2.789091735509277	0.0
68-69	0.0	0.0	0.0	3.057172902907447	0.0
70-71	0.0	0.0	0.0	3.3460056020548885	0.0
72-73	0.0	0.0	0.0	3.6298293107800914	0.0
74-75	0.0	0.0	0.0	3.908271932651691	0.0
76-77	0.0	0.0	0.0	4.215194242924014	0.0
78-79	0.0	0.0	0.0	4.537744603313719	0.0
80-81	0.0	0.0	0.0	4.890377529039265	0.0
82-83	0.0	0.0	0.0	5.2495936993197505	0.0
84-85	0.0	0.0	0.0	5.607521843491661	0.0
86-87	0.0	0.0	0.0	5.977872283910722	0.0
88	0.0	0.0	0.0	6.271885710294878	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2575	0.0	32.32591	1
GTATCAA	3635	0.0	30.92062	1
GTACTAG	955	0.0	30.53114	1
CAACGCA	3910	0.0	27.400097	5
ATCAACG	4090	0.0	26.08083	3
TACATGG	5550	0.0	25.570124	2
GCATCGT	905	0.0	25.442886	8
TATCAAC	4410	0.0	24.93424	2
GTACATG	6015	0.0	24.628029	1
AACGCAG	4480	0.0	24.54324	6
ACATGGG	5750	0.0	23.863487	3
TAACGCC	265	3.6379788E-11	23.051092	4
TAGGCAT	1380	0.0	22.813385	5
GTCCTAC	910	0.0	22.22193	1
TCAACGC	4825	0.0	22.204018	4
ACGCAGA	4885	0.0	21.835108	7
TAGTACT	1340	0.0	21.74107	4
CGCAGAG	4845	0.0	21.725668	8
CTGTGCG	1215	0.0	21.658613	9
CTAGTAC	1370	0.0	21.266207	3
>>END_MODULE