FastQCFastQC Report
Mon 16 Jan 2017
ERR1146495_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146495_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746859
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76350.437070192843269No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67430.3860071133388556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37990.21747605273236137No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT20650.11821217396481341No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46400.051.7397771
GTATCAA81950.040.4960371
CAACGCA98200.033.1786775
ATCAACG98150.033.1476753
TATCAAC101650.032.4597852
AACGCAG105900.031.01936
TCAACGC108200.030.1991584
ACGCAGA115300.028.1643357
CGCAGAG115750.028.0142428
GTACTAG9150.027.2663571
GCAGAGT119650.026.5127189
TACATGG50350.023.9318082
GAGTACT88700.023.52461612-13
ACATGGG49400.023.222033
GCTATTG10950.023.175578
GTACATG53400.023.0957661
CAGAGTA117850.022.88998410-11
AGAGTAC112350.022.06544110-11
GGGCTAT13300.021.90746
CATGGGG37500.021.8150024
Produced by FastQC (version 0.11.5)