##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146495_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1746859 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.520443836623336 34.0 31.0 34.0 31.0 34.0 2 32.6060987177557 34.0 31.0 34.0 31.0 34.0 3 32.72975094154709 34.0 31.0 34.0 31.0 34.0 4 36.19862450260725 37.0 37.0 37.0 35.0 37.0 5 36.229992231771426 37.0 37.0 37.0 35.0 37.0 6 36.304322787357194 37.0 37.0 37.0 35.0 37.0 7 36.288162353114934 37.0 37.0 37.0 35.0 37.0 8 36.29767600018089 37.0 37.0 37.0 35.0 37.0 9 38.11953740971653 39.0 39.0 39.0 37.0 39.0 10-11 38.083986171751704 39.0 38.0 39.0 36.0 39.0 12-13 38.0563585841788 39.0 38.0 39.0 35.0 39.0 14-15 39.50009788998425 41.0 39.0 41.0 37.0 41.0 16-17 39.500904480556244 41.0 39.0 41.0 37.0 41.0 18-19 39.56147118914578 41.0 39.5 41.0 37.0 41.0 20-21 39.59506777593383 41.0 40.0 41.0 37.0 41.0 22-23 39.55018579061046 41.0 39.5 41.0 37.0 41.0 24-25 39.5277984657033 41.0 39.0 41.0 36.5 41.0 26-27 39.448241958853 41.0 39.0 41.0 36.0 41.0 28-29 39.35440782570316 41.0 39.0 41.0 36.0 41.0 30-31 39.23172305263333 41.0 39.0 41.0 35.5 41.0 32-33 39.087745204392576 41.0 39.0 41.0 35.0 41.0 34-35 38.98750328446658 41.0 39.0 41.0 35.0 41.0 36-37 38.88858230687194 41.0 39.0 41.0 35.0 41.0 38-39 38.7755385523388 41.0 38.0 41.0 35.0 41.0 40-41 38.67520618435718 40.0 38.0 41.0 35.0 41.0 42-43 38.566032232710256 40.0 38.0 41.0 35.0 41.0 44-45 38.38569999066897 40.0 38.0 41.0 34.5 41.0 46-47 38.10595617619968 40.0 38.0 41.0 34.0 41.0 48-49 37.850732085417306 40.0 38.0 41.0 33.0 41.0 50-51 37.67985338255692 40.0 37.0 41.0 33.0 41.0 52-53 37.54724079047021 40.0 37.0 41.0 33.0 41.0 54-55 37.409221637235746 40.0 36.5 41.0 33.0 41.0 56-57 37.21365920203061 40.0 36.0 41.0 32.5 41.0 58-59 37.00750718861683 39.5 35.5 41.0 32.0 41.0 60-61 36.829568671541324 39.0 35.0 41.0 32.0 41.0 62-63 36.57923392786711 39.0 35.0 41.0 31.5 41.0 64-65 36.29220360658759 38.5 35.0 41.0 31.0 41.0 66-67 36.041388572288895 37.5 35.0 40.5 31.0 41.0 68-69 35.738658930114 37.0 35.0 40.0 31.0 41.0 70-71 35.47716301086693 36.5 35.0 39.5 31.0 41.0 72-73 35.164845874795844 36.0 35.0 39.0 30.5 41.0 74-75 34.841495507078704 35.5 35.0 39.0 30.0 41.0 76-77 34.54001839873739 35.0 35.0 37.5 30.0 39.5 78-79 34.24767625778612 35.0 35.0 37.0 30.0 39.0 80-81 33.985283013683414 35.0 35.0 37.0 30.0 39.0 82-83 33.725136087114066 35.0 34.0 36.0 29.0 38.0 84-85 33.51198951947467 35.0 34.0 36.0 29.0 37.0 86-87 33.32530616380601 35.0 34.0 36.0 29.0 37.0 88-89 33.16948276878672 35.0 34.0 35.0 29.0 36.5 90-91 33.071772821962156 35.0 34.0 35.0 29.0 36.0 92-93 32.986464562967015 35.0 34.0 35.0 29.0 36.0 94-95 32.90905390761361 35.0 34.0 35.0 29.0 36.0 96-97 32.85603503202033 35.0 34.0 35.0 29.0 36.0 98-99 32.80206816921114 35.0 34.0 35.0 29.0 35.0 100 32.2537617518071 35.0 33.0 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31805701384360674 1101 2 0.3700709398882722 1101 3 0.36473350067596044 1101 4 0.2526505060398989 1101 5 0.1945113363083948 1101 6 0.23843456760419457 1101 7 0.2092197456287508 1101 8 0.2143804776894669 1101 9 0.17907007924574714 1101 10-11 0.23891369833103226 1101 12-13 0.2903357319026654 1101 14-15 0.38117743391655523 1101 16-17 0.3750043253721742 1101 18-19 0.3459206738628282 1101 20-21 0.36725275745435937 1101 22-23 0.34710587399094095 1101 24-25 0.3251093844506556 1101 26-27 0.4131924359132313 1101 28-29 0.4885107252764769 1101 30-31 0.4935315021337985 1101 32-33 0.44852699614218494 1101 34-35 0.4699500442986846 1101 36-37 0.47919792864149 1101 38-39 0.5411154580366144 1101 40-41 0.4908222767135726 1101 42-43 0.45667832451305657 1101 44-45 0.5396939423713576 1101 46-47 0.5227823120193307 1101 48-49 0.4747671717087698 1101 50-51 0.4754981932680096 1101 52-53 0.4529466355935696 1101 54-55 0.3632884000489085 1101 56-57 0.412506612800577 1101 58-59 0.40378021237706463 1101 60-61 0.35535199178640653 1101 62-63 0.3342575350262251 1101 64-65 0.3969042670942855 1101 66-67 0.36103444134091234 1101 68-69 0.34107040118831833 1101 70-71 0.32244694559477693 1101 72-73 0.31722577562720744 1101 74-75 0.3284331341789155 1101 76-77 0.40088240963186195 1101 78-79 0.35219415137597565 1101 80-81 0.34708192060201526 1101 82-83 0.2800566874234107 1101 84-85 0.2662552549515951 1101 86-87 0.36473087321872555 1101 88-89 0.3632152934895174 1101 90-91 0.3069295553383995 1101 92-93 0.2182701355540857 1101 94-95 0.22287989242674655 1101 96-97 0.2732363361718768 1101 98-99 0.2944758199240667 1101 100 0.2968068864438038 1102 1 0.2997206989956638 1102 2 0.2412695087612633 1102 3 0.2547156115883027 1102 4 0.26606732178230175 1102 5 0.14173852772162832 1102 6 0.18879234935732825 1102 7 0.139005076576872 1102 8 0.2210888855606612 1102 9 0.14329190393268476 1102 10-11 0.1906131616584048 1102 12-13 0.22414610757350317 1102 14-15 0.3127516736303235 1102 16-17 0.29897570283192465 1102 18-19 0.24574178298626492 1102 20-21 0.2605890723023023 1102 22-23 0.32630980959022793 1102 24-25 0.28016305171362177 1102 26-27 0.2904285718702937 1102 28-29 0.3107379166897246 1102 30-31 0.3039071729745828 1102 32-33 0.3174894290581065 1102 34-35 0.3221414575366168 1102 36-37 0.32736429715669857 1102 38-39 0.4456324526698836 1102 40-41 0.3770029564989059 1102 42-43 0.3059627610067892 1102 44-45 0.3274846400457747 1102 46-47 0.22425816175099555 1102 48-49 0.2609925742132404 1102 50-51 0.2796126834290149 1102 52-53 0.4039752581338192 1102 54-55 0.3474118347537356 1102 56-57 0.26849945716551105 1102 58-59 0.26402171506042293 1102 60-61 0.2773108468847951 1102 62-63 0.31256701624268146 1102 64-65 0.32355900770251367 1102 66-67 0.29417397622463426 1102 68-69 0.2751043904548638 1102 70-71 0.24731277743735092 1102 72-73 0.22845117813167803 1102 74-75 0.17905925224689412 1102 76-77 0.17816326830806872 1102 78-79 0.19141597606290617 1102 80-81 0.24869193849109905 1102 82-83 0.21878656219979575 1102 84-85 0.2163894231090211 1102 86-87 0.15386325246738153 1102 88-89 0.11232083910669388 1102 90-91 0.20988125480172926 1102 92-93 0.17399464360418193 1102 94-95 0.11934679761458256 1102 96-97 0.15024885853323866 1102 98-99 0.18714129398489376 1102 100 0.2024419490556113 1103 1 0.22784613658603092 1103 2 0.27362287567007115 1103 3 0.22058552944324816 1103 4 0.10406723682093144 1103 5 0.17756508381265235 1103 6 0.11871456250651136 1103 7 0.18912441582918404 1103 8 0.16383003817173147 1103 9 0.21852165362706444 1103 10-11 0.22502369497978236 1103 12-13 0.24502083990586954 1103 14-15 0.2397185292573667 1103 16-17 0.26395195298336205 1103 18-19 0.23354786024132324 1103 20-21 0.26544976712894197 1103 22-23 0.25943774095402716 1103 24-25 0.2135773343486207 1103 26-27 0.2533205151727458 1103 28-29 0.2592517221250432 1103 30-31 0.2538725984186243 1103 32-33 0.25362719548672885 1103 34-35 0.24715931515709144 1103 36-37 0.1974506981460209 1103 38-39 0.18103896133987263 1103 40-41 0.2214611153861057 1103 42-43 0.24982414496451355 1103 44-45 0.3589687263098824 1103 46-47 0.26730879400516017 1103 48-49 0.2948028712161417 1103 50-51 0.26996111156507396 1103 52-53 0.20576216086721644 1103 54-55 0.3067477908759102 1103 56-57 0.31150190531155175 1103 58-59 0.24650767676802587 1103 60-61 0.22934189554406004 1103 62-63 0.23868445487775602 1103 64-65 0.30531394765528574 1103 66-67 0.3065901358764833 1103 68-69 0.2560400038995283 1103 70-71 0.2802681506930895 1103 72-73 0.23247602618457108 1103 74-75 0.25892509982305967 1103 76-77 0.22843719004606555 1103 78-79 0.25849887475592226 1103 80-81 0.27937380852223725 1103 82-83 0.23615860487557683 1103 84-85 0.22911297770377814 1103 86-87 0.2918493612840152 1103 88-89 0.1883665719220744 1103 90-91 0.14948375360991406 1103 92-93 0.15969497699887114 1103 94-95 0.17105719578444933 1103 96-97 0.18883134893967934 1103 98-99 0.226093130330419 1103 100 0.1519611306573836 1104 1 0.2159598463689747 1104 2 0.20243562076489496 1104 3 0.16650602532072156 1104 4 0.1418856937937747 1104 5 0.1178342065394844 1104 6 0.10832722566463104 1104 7 0.13431484395942306 1104 8 0.11163165219369375 1104 9 0.15991048085687964 1104 10-11 0.17614842940533748 1104 12-13 0.13620183656680496 1104 14-15 0.18887557984198367 1104 16-17 0.19852213698740684 1104 18-19 0.23318884040556753 1104 20-21 0.1710239965757907 1104 22-23 0.21751998820678864 1104 24-25 0.18726582759886412 1104 26-27 0.2759792228981581 1104 28-29 0.305431240790611 1104 30-31 0.2498489337263834 1104 32-33 0.1851261197029288 1104 34-35 0.21435182742482084 1104 36-37 0.1819462351831973 1104 38-39 0.2291858904810269 1104 40-41 0.1962792454139759 1104 42-43 0.16294161807720542 1104 44-45 0.1277418188484276 1104 46-47 0.1867398222853538 1104 48-49 0.14419733050129224 1104 50-51 0.08880152487891024 1104 52-53 0.10195576696086306 1104 54-55 0.06535743280009854 1104 56-57 0.0768336924904176 1104 58-59 0.10829870827028287 1104 60-61 0.08515643136421858 1104 62-63 0.09185919256547947 1104 64-65 0.13579753826186902 1104 66-67 0.11716653219939843 1104 68-69 0.10223727308895292 1104 70-71 0.007144084007975948 1104 72-73 0.0635875370165877 1104 74-75 -0.032690219717359525 1104 76-77 0.03301367238793773 1104 78-79 0.025524342566058067 1104 80-81 -0.006931206711506377 1104 82-83 -0.010179210556160001 1104 84-85 0.060678092785124704 1104 86-87 0.07214179660850562 1104 88-89 0.06794776526027135 1104 90-91 0.04024230967222309 1104 92-93 0.02672669576350728 1104 94-95 0.060884213831300826 1104 96-97 0.045927694919619455 1104 98-99 0.01364614954144372 1104 100 0.019731755982462573 1105 1 0.16215292881952337 1105 2 0.14033825789010024 1105 3 0.20429972821619913 1105 4 0.11926883292994006 1105 5 0.09726803966081121 1105 6 0.11460445399368524 1105 7 0.14748415746631593 1105 8 0.11888763224121135 1105 9 0.1702181585701652 1105 10-11 0.19652696037763917 1105 12-13 0.1819055264273146 1105 14-15 0.15088729775870746 1105 16-17 0.19191489365584147 1105 18-19 0.14270240749380747 1105 20-21 0.16559752935585692 1105 22-23 0.19258833770180672 1105 24-25 0.21116259455607178 1105 26-27 0.27131082816307384 1105 28-29 0.25176712060726203 1105 30-31 0.2055533915305574 1105 32-33 0.12933551501672724 1105 34-35 0.21939340262281348 1105 36-37 0.24227548726128845 1105 38-39 0.27374870718458766 1105 40-41 0.21546185890191083 1105 42-43 0.2538265786117577 1105 44-45 0.28853525020304716 1105 46-47 0.3564342894604522 1105 48-49 0.36497226428497953 1105 50-51 0.3781201145863591 1105 52-53 0.3695265771380676 1105 54-55 0.4525498558898775 1105 56-57 0.4308542121456824 1105 58-59 0.4544389381280354 1105 60-61 0.42376755088719875 1105 62-63 0.4161074234090023 1105 64-65 0.34765529100442194 1105 66-67 0.39376896208019474 1105 68-69 0.37149187083789315 1105 70-71 0.3899743696311475 1105 72-73 0.41496450683807495 1105 74-75 0.39594087805225087 1105 76-77 0.4069875934229117 1105 78-79 0.38572689230630175 1105 80-81 0.39778154137068356 1105 82-83 0.4035014128869392 1105 84-85 0.41037345003870485 1105 86-87 0.39075400697955587 1105 88-89 0.405126728422573 1105 90-91 0.38874911099281917 1105 92-93 0.2933590754469009 1105 94-95 0.31739044496627855 1105 96-97 0.2767443703727466 1105 98-99 0.3480153423858141 1105 100 0.3987086040706558 1106 1 0.14081979729083116 1106 2 0.2088066707962426 1106 3 0.2082179268517521 1106 4 0.15823920761004473 1106 5 0.1486811808559878 1106 6 0.11003191115136701 1106 7 0.061109463516579865 1106 8 0.11295227874273195 1106 9 0.1425525673208483 1106 10-11 0.13422140318758125 1106 12-13 0.1882394808028991 1106 14-15 0.24511073954945317 1106 16-17 0.18720515722172593 1106 18-19 0.18298217858190213 1106 20-21 0.21390123757906565 1106 22-23 0.20445172875608364 1106 24-25 0.16396485233465086 1106 26-27 0.1664323058513375 1106 28-29 0.17538214972471877 1106 30-31 0.2432562988075162 1106 32-33 0.186264326331802 1106 34-35 0.23660899574439043 1106 36-37 0.25864416168484894 1106 38-39 0.31049228197759504 1106 40-41 0.26088214171579693 1106 42-43 0.20438606729502595 1106 44-45 0.20264655511234508 1106 46-47 0.2046603385587673 1106 48-49 0.19672013581764958 1106 50-51 0.12423330370489083 1106 52-53 0.06250509721569841 1106 54-55 0.16609223218986813 1106 56-57 0.14891847035156758 1106 58-59 0.16158002191335186 1106 60-61 0.08006692366704726 1106 62-63 0.15180121549337855 1106 64-65 0.21281467943566668 1106 66-67 0.22186539155404006 1106 68-69 0.030526540854260986 1106 70-71 0.002762291308712861 1106 72-73 0.03549599984732765 1106 74-75 0.08006152219504514 1106 76-77 0.05357406896673922 1106 78-79 0.12046127324067157 1106 80-81 0.028507839840379745 1106 82-83 0.09390786778250515 1106 84-85 0.03547103527415629 1106 86-87 0.11330797267675763 1106 88-89 0.1824098453351084 1106 90-91 0.15901883485295798 1106 92-93 0.0901681287135645 1106 94-95 0.05825967650280717 1106 96-97 0.015881139482537776 1106 98-99 -0.014860213271830958 1106 100 0.04791292843784589 1107 1 0.09761803602422248 1107 2 0.05348275039371231 1107 3 0.0927405821593652 1107 4 0.025690816735348676 1107 5 0.08658127986043951 1107 6 0.09709687168160741 1107 7 0.10499263763612987 1107 8 0.09069087653269747 1107 9 0.12914052332148884 1107 10-11 0.14783692355742062 1107 12-13 0.16442505684450026 1107 14-15 0.1495589349314983 1107 16-17 0.2270995731777532 1107 18-19 0.12001532845513907 1107 20-21 0.13668280926783183 1107 22-23 0.16435795255462438 1107 24-25 0.1431400554304716 1107 26-27 0.08971161802043781 1107 28-29 0.16687442989334755 1107 30-31 0.1725950130294578 1107 32-33 0.10758925572860534 1107 34-35 0.058107097511772565 1107 36-37 0.10747566367907524 1107 38-39 0.050458567030283064 1107 40-41 0.06177266925391933 1107 42-43 -0.0762241567868287 1107 44-45 -0.09339090566408714 1107 46-47 -0.015930646909922075 1107 48-49 0.044870613272628646 1107 50-51 0.025713928249984974 1107 52-53 -0.054218268426375005 1107 54-55 0.06809296408350463 1107 56-57 4.5395585395624494E-4 1107 58-59 0.07774232087948008 1107 60-61 0.16790239174191157 1107 62-63 0.04153806928891868 1107 64-65 0.023464927785560974 1107 66-67 0.06424358609422853 1107 68-69 0.034447499448532426 1107 70-71 -0.05407316340816237 1107 72-73 -0.07878347479221048 1107 74-75 -0.07799558290469832 1107 76-77 -0.06872004478804428 1107 78-79 -0.041102770403298905 1107 80-81 -0.02229182229564941 1107 82-83 -0.006081818606048728 1107 84-85 -0.04412736760719582 1107 86-87 0.029516713959502283 1107 88-89 0.032988991896189646 1107 90-91 -0.0290574032386246 1107 92-93 0.0562893130268165 1107 94-95 0.08999896145266462 1107 96-97 0.03909204631303709 1107 98-99 -1.6463722090520605E-4 1107 100 -0.06731946798258548 1108 1 0.057441307377246176 1108 2 0.03547823744297318 1108 3 0.024191237272845 1108 4 0.04816207221253421 1108 5 -0.026992019889775065 1108 6 -0.017768867183868053 1108 7 -0.038081284225008005 1108 8 0.021247348611268535 1108 9 -0.08146013555509768 1108 10-11 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2214 60-61 -0.27961988234273605 2214 62-63 -0.25601999304346634 2214 64-65 -0.23798080225963503 2214 66-67 -0.2813338176643114 2214 68-69 -0.2282602075800284 2214 70-71 -0.22645729160194605 2214 72-73 -0.3674512431878014 2214 74-75 -0.3215857437527845 2214 76-77 -0.1985467014872455 2214 78-79 -0.20727077162903385 2214 80-81 -0.14522251301509215 2214 82-83 -0.1290396102546083 2214 84-85 -0.20421005235417056 2214 86-87 -0.2008806144521813 2214 88-89 -0.1463843595161194 2214 90-91 -0.21113033265253733 2214 92-93 -0.18454587877516104 2214 94-95 -0.10480195374508128 2214 96-97 -0.09412034886332066 2214 98-99 -0.20045045501053949 2214 100 -0.19922200578118776 2215 1 -0.2925065289685449 2215 2 -0.308595670385472 2215 3 -0.35391229643275324 2215 4 -0.2686205903951233 2215 5 -0.18401491825134286 2215 6 -0.20209387583631155 2215 7 -0.20345398895065614 2215 8 -0.23535776516978046 2215 9 -0.2940124831232609 2215 10-11 -0.2753286471131986 2215 12-13 -0.2887254460619104 2215 14-15 -0.34443693562625555 2215 16-17 -0.3965751045789432 2215 18-19 -0.4000895269899516 2215 20-21 -0.4217139675894259 2215 22-23 -0.3796141623093945 2215 24-25 -0.40138168614805636 2215 26-27 -0.42313664079889435 2215 28-29 -0.40755203064555445 2215 30-31 -0.3951631009592518 2215 32-33 -0.39155469004791854 2215 34-35 -0.3922191302864846 2215 36-37 -0.36910537546151545 2215 38-39 -0.4110967631447764 2215 40-41 -0.44147919670812996 2215 42-43 -0.4492102451640534 2215 44-45 -0.3808845915589032 2215 46-47 -0.29743666482511344 2215 48-49 -0.2781762254634472 2215 50-51 -0.2759049722546152 2215 52-53 -0.3528113426993329 2215 54-55 -0.3866907136841249 2215 56-57 -0.2735765803336889 2215 58-59 -0.21122975559384827 2215 60-61 -0.27482806349954814 2215 62-63 -0.26663109730968415 2215 64-65 -0.25449140548006 2215 66-67 -0.3332580990244054 2215 68-69 -0.2648868059836076 2215 70-71 -0.30110015345938734 2215 72-73 -0.37524900681621176 2215 74-75 -0.25844055194435356 2215 76-77 -0.29261553881457303 2215 78-79 -0.30422610433459596 2215 80-81 -0.2649346536951427 2215 82-83 -0.18150540170455542 2215 84-85 -0.19077020645215015 2215 86-87 -0.2357218888334316 2215 88-89 -0.36410313435939656 2215 90-91 -0.3376212959336229 2215 92-93 -0.2869215377178591 2215 94-95 -0.20892892617289505 2215 96-97 -0.16418462236157438 2215 98-99 -0.18373001319257298 2215 100 -0.2237733967113158 2216 1 -0.35185182995530084 2216 2 -0.3783909576222513 2216 3 -0.36557583058474563 2216 4 -0.26151608846495833 2216 5 -0.2642534718221654 2216 6 -0.29639635200473435 2216 7 -0.3533904771294658 2216 8 -0.2737693217711197 2216 9 -0.4116895839240655 2216 10-11 -0.3690285708163117 2216 12-13 -0.36825858076142026 2216 14-15 -0.45007323930029486 2216 16-17 -0.5492571953481189 2216 18-19 -0.49212394536571935 2216 20-21 -0.43484287418672807 2216 22-23 -0.5089701324550475 2216 24-25 -0.4863825063224212 2216 26-27 -0.4812038654598183 2216 28-29 -0.48096271882698005 2216 30-31 -0.4495568577093181 2216 32-33 -0.48341447676122584 2216 34-35 -0.5234577107369773 2216 36-37 -0.5361780801277618 2216 38-39 -0.5077231241000604 2216 40-41 -0.5119928257315465 2216 42-43 -0.4750477189994555 2216 44-45 -0.4166026679950221 2216 46-47 -0.40449733222648376 2216 48-49 -0.44874087415657726 2216 50-51 -0.434896116439198 2216 52-53 -0.3680183402339807 2216 54-55 -0.39995100325391775 2216 56-57 -0.3381829724556056 2216 58-59 -0.4360622918417647 2216 60-61 -0.39950335371376156 2216 62-63 -0.5209110807132191 2216 64-65 -0.35410080605666394 2216 66-67 -0.35630318931595895 2216 68-69 -0.4172384147314929 2216 70-71 -0.40138947168772887 2216 72-73 -0.36863540336293 2216 74-75 -0.3634405312676918 2216 76-77 -0.3506770773927457 2216 78-79 -0.3618946139812991 2216 80-81 -0.38042709258353824 2216 82-83 -0.28516984080523144 2216 84-85 -0.36650970735882993 2216 86-87 -0.4199263300431255 2216 88-89 -0.3211669204910024 2216 90-91 -0.3522202272257573 2216 92-93 -0.33296562656962436 2216 94-95 -0.28760777001652116 2216 96-97 -0.2957327497105311 2216 98-99 -0.35571041033615813 2216 100 -0.3856396310978525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 10.0 8 43.0 9 101.0 10 234.0 11 214.0 12 149.0 13 154.0 14 222.0 15 387.0 16 832.0 17 1560.0 18 2819.0 19 4360.0 20 6404.0 21 9179.0 22 13528.0 23 15706.0 24 14829.0 25 11967.0 26 10264.0 27 10465.0 28 12048.0 29 13206.0 30 16296.0 31 20995.0 32 27061.0 33 36545.0 34 53670.0 35 87796.0 36 158591.0 37 394294.0 38 578565.0 39 243692.0 40 672.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.59950284359267 20.333662709948968 13.704204532573364 23.362629913884998 2 15.287386618229327 21.300989565725615 40.19138097993938 23.220242836105683 3 17.346794492724918 27.12231653149979 31.491373343950524 24.039515631824774 4 11.836444727364945 18.436233262100718 39.653744234652024 30.07357777588231 5 12.075932433237277 38.63613456542953 35.812548963912164 13.475384037421028 6 28.35842342332027 39.541165074777936 18.395016636401316 13.705394865500478 7 24.71716377795804 34.180320220464274 23.658062843080067 17.444453158497623 8 25.036136288046144 34.757298671501246 21.21590809561619 18.990656944836417 9 26.108804431267778 14.575704163873556 21.41838579988425 37.89710560497441 10-11 23.97271331000384 26.42895047625481 29.143451188676362 20.45488502506499 12-13 25.21677479407325 24.705313937759144 28.879692064442526 21.198219203725085 14-15 22.413034598967233 25.484634273468632 27.5429292069163 24.559401920647836 16-17 20.807632442000184 28.247242622329566 28.372810856514462 22.572314079155788 18-19 20.511443682632656 27.207261719463332 31.345517869501776 20.935776728402235 20-21 21.809430526447755 26.20574986303989 31.10706130260084 20.877758307911513 22-23 21.79148181011535 26.23562412342216 30.62632738930074 21.34656667716175 24-25 21.505370496416713 26.335382535167405 31.066760396803634 21.09248657161225 26-27 21.373848719329953 26.495727474283843 30.609024540618336 21.521399265767872 28-29 20.99779174889766 26.733147952823856 30.839893923803174 21.429166374475308 30-31 21.634330944946505 26.206489317519395 30.872330310091478 21.286849427442625 32-33 21.685970709758116 26.575806712454437 30.350392354139146 21.3878302236483 34-35 21.667199058410816 26.58775819568431 30.703976451110982 21.041066294793893 36-37 21.76311780738166 26.320138673023273 30.389823241126994 21.52692027846807 38-39 21.75564467492975 26.227820376787758 30.640546058595337 21.375988889687154 40-41 21.655895259502707 26.108642991610807 30.66910002481636 21.566361724070124 42-43 21.69019002830818 26.217065624025025 30.143201028139305 21.94954331952749 44-45 21.78255768456298 26.71289083575889 29.86814261904074 21.636408860637392 46-47 22.02392588956423 26.538416952776494 29.1752165680846 22.262440589574677 48-49 22.402655299134754 26.58439078648296 28.985546240038833 22.02740767434345 50-51 22.20103798621972 26.805599126195585 28.637760441115525 22.355602446469174 52-53 21.90593272976555 27.05576084747558 28.57882971202203 22.459476710736848 54-55 21.89207825258375 27.060725124733054 28.5808783393695 22.466318283313694 56-57 22.464071562343555 26.944703881092753 28.153200020265157 22.438024536298535 58-59 21.761153187810926 27.123528031096683 28.68173985722546 22.433578923866936 60-61 21.921762559296916 27.403884822937176 28.28720245634433 22.387150161421573 62-63 22.037040058368305 27.104823943369695 28.16064564640635 22.697490351855652 64-65 21.670813348920127 27.054869021707397 28.409505958971316 22.86481167040116 66-67 22.060613797783983 26.965809384750976 28.17635072065437 22.797226096810675 68-69 21.838029602222814 26.726355046343457 28.50681634273522 22.928799008698505 70-71 21.997488019720304 26.56722552694053 28.35234587930473 23.082940574034435 72-73 22.251387205223363 26.567531168286564 28.354889817906503 22.82619180858357 74-75 22.24359469771782 26.8723862845955 28.139106191625235 22.744912826061444 76-77 22.155111686516925 26.67398105034588 27.92879954132918 23.24210772180802 78-79 22.0827738718437 26.820662526436973 27.892824972586265 23.20373862913306 80-81 21.958990954333167 26.990251356935897 28.014318274777267 23.036439413953676 82-83 22.32545851674623 26.54712997154029 28.43094348673946 22.696468024974017 84-85 22.505934944875282 26.732296919553235 27.753387941886487 23.008380193685003 86-87 21.776915022906827 26.899566593525865 28.099835189903704 23.2236831936636 88-89 22.238381412091208 26.513576390956096 27.889362374460173 23.358679822492523 90-91 22.104423455019393 26.445072683334107 28.020472179057432 23.43003168258906 92-93 22.273205793942154 26.545330790865208 27.901135695554135 23.280327719638507 94-95 21.69433823794594 26.641617898181824 28.148264971586144 23.5157788922861 96-97 21.939195799532072 26.45357107896314 27.947514759398757 23.659718362106027 98-99 21.730292163119948 26.71171729447786 27.842953843476497 23.715036698925694 100 21.996418692410558 26.31688618586162 27.676306123944382 24.010388997783437 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 426.0 1 269.5 2 275.0 3 391.5 4 225.5 5 181.0 6 415.0 7 1013.5 8 1877.0 9 2250.5 10 1573.0 11 914.0 12 878.0 13 956.5 14 1167.0 15 1401.5 16 1675.0 17 2019.5 18 2326.0 19 2760.0 20 3278.0 21 3853.0 22 4558.5 23 5350.0 24 6221.5 25 7460.5 26 9667.0 27 12371.0 28 14696.5 29 17836.0 30 22164.0 31 26560.0 32 31196.5 33 34904.0 34 38616.0 35 42185.0 36 45815.5 37 50992.0 38 53546.5 39 53753.0 40 54241.5 41 54078.5 42 54275.0 43 56192.0 44 59422.5 45 64948.0 46 69255.0 47 71380.5 48 71924.5 49 69632.5 50 67190.0 51 66992.5 52 65512.0 53 61816.0 54 58215.5 55 51668.5 56 46043.0 57 39529.0 58 32730.0 59 30207.0 60 25313.0 61 18856.0 62 13986.0 63 10303.5 64 7226.5 65 4494.0 66 3121.0 67 2243.0 68 1666.0 69 1381.5 70 1107.0 71 820.0 72 652.5 73 566.5 74 478.5 75 397.0 76 334.0 77 245.5 78 162.5 79 114.5 80 83.5 81 66.0 82 54.0 83 37.0 84 29.0 85 25.0 86 14.0 87 7.5 88 5.0 89 3.0 90 1.0 91 1.5 92 2.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007155700603196938 2 0.094398002357374 3 3.43473628953453E-4 4 0.0 5 0.038640783257263465 6 0.004980367619825069 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.8622802412787753E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.152104434301795E-4 24-25 0.0 26-27 0.0 28-29 8.586840723836326E-5 30-31 2.0035961688951427E-4 32-33 0.0018032365520056282 34-35 0.0014311401206393876 36-37 9.159296772092081E-4 38-39 7.728156651452694E-4 40-41 0.0015742541327033264 42-43 8.014384675580571E-4 44-45 5.438332458429673E-4 46-47 3.720964313662408E-4 48-49 7.155700603196938E-4 50-51 8.586840723836325E-4 52-53 0.001373894515813812 54-55 0.0016314997375289021 56-57 0.0010590436892731468 58-59 0.0028050346364531997 60-61 0.0020322189713079305 62-63 0.0011735348989242978 64-65 0.004636893990871616 66-67 0.004493779978807677 68-69 0.00369234151124962 70-71 0.001717368144767265 72-73 0.005237972841540158 74-75 0.0036637187088368324 76-77 0.004150306349854224 78-79 8.873068747964203E-4 80-81 2.8622802412787753E-5 82-83 2.0035961688951427E-4 84-85 1.1449120965115101E-4 86-87 0.0 88-89 1.717368144767265E-4 90-91 2.8622802412787753E-5 92-93 0.0 94-95 0.0 96-97 3.43473628953453E-4 98-99 5.438332458429673E-4 100 6.297016530813305E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1746859.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.120681039620436 #Duplication Level Percentage of deduplicated Percentage of total 1 69.02378495555173 38.04638034682319 2 16.136066282490894 17.788619255827086 3 6.506750410779836 10.759695419910438 4 3.1727290234695693 6.995319381112501 5 1.6778265803930215 4.6241471888820405 6 1.0309244363852819 3.4095154220366153 7 0.6635994791275771 2.5604638658934604 8 0.45404659510443124 2.0021886036701635 9 0.29468407315332334 1.4618868123366462 >10 1.0081090857433779 8.241437550209467 >50 0.018389163864636526 0.6947233553318761 >100 0.010983499064660372 1.1337880758989616 >500 0.0012036656409262722 0.42851784578479396 >1k 6.018328204631361E-4 0.6608977575109394 >5k 3.0091641023156804E-4 1.1924191187717763 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7635 0.437070192843269 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6743 0.3860071133388556 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3799 0.21747605273236137 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 2065 0.11821217396481341 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.8622802412787753E-5 0.0 20-21 0.0 0.0 0.0 8.586840723836326E-5 0.0 22-23 0.0 0.0 0.0 6.583244554941183E-4 0.0 24-25 0.0 0.0 0.0 0.0020894645761335056 0.0 26-27 0.0 0.0 0.0 0.0031771310678194407 0.0 28-29 0.0 0.0 0.0 0.010361454473429167 0.0 30-31 0.0 0.0 0.0 0.029195258461043507 0.0 32-33 0.0 0.0 0.0 0.0633136389370865 0.0 34-35 0.0 0.0 0.0 0.11763971791655767 0.0 36-37 0.0 0.0 0.0 0.19918608199058996 0.0 38-39 0.0 0.0 0.0 0.3086969240219159 0.0 40-41 0.0 0.0 0.0 0.4227301688344623 0.0 42-43 0.0 0.0 0.0 0.5333000545550615 0.0 44-45 0.0 0.0 0.0 0.66347655992842 0.0 46-47 0.0 0.0 0.0 0.8201291575336074 0.0 48-49 0.0 0.0 0.0 0.9851109906409161 0.0 50-51 0.0 0.0 0.0 1.1555597790090673 0.0 52-53 0.0 0.0 0.0 1.3191390947981492 0.0 54-55 0.0 0.0 0.0 1.4866683573201958 0.0 56-57 0.0 0.0 0.0 1.683106650279158 0.0 58-59 0.0 0.0 0.0 1.8962320370447758 0.0 60-61 0.0 0.0 0.0 2.1194326502596947 0.0 62-63 0.0 0.0 0.0 2.3410017637370846 0.0 64-65 0.0 0.0 0.0 2.5636871665085734 0.0 66-67 0.0 0.0 0.0 2.807238592238984 0.0 68-69 0.0 0.0 0.0 3.0781534170760203 0.0 70-71 0.0 0.0 0.0 3.3701060016864557 0.0 72-73 0.0 0.0 0.0 3.656849236257763 0.0 74-75 0.0 0.0 0.0 3.937553059519973 0.0 76-77 0.0 0.0 0.0 4.247423518440813 0.0 78-79 0.0 0.0 0.0 4.571719869777699 0.0 80-81 0.0 0.0 0.0 4.926757110905918 0.0 82-83 0.0 0.0 0.0 5.288577956205968 0.0 84-85 0.0 0.0 0.0 5.648824547373314 0.0 86-87 0.0 0.0 0.0 6.022323496057782 0.0 88 0.0 0.0 0.0 6.318712615042198 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4640 0.0 51.739777 1 GTATCAA 8195 0.0 40.496037 1 CAACGCA 9820 0.0 33.178677 5 ATCAACG 9815 0.0 33.147675 3 TATCAAC 10165 0.0 32.459785 2 AACGCAG 10590 0.0 31.0193 6 TCAACGC 10820 0.0 30.199158 4 ACGCAGA 11530 0.0 28.164335 7 CGCAGAG 11575 0.0 28.014242 8 GTACTAG 915 0.0 27.266357 1 GCAGAGT 11965 0.0 26.512718 9 TACATGG 5035 0.0 23.931808 2 GAGTACT 8870 0.0 23.524616 12-13 ACATGGG 4940 0.0 23.22203 3 GCTATTG 1095 0.0 23.17557 8 GTACATG 5340 0.0 23.095766 1 CAGAGTA 11785 0.0 22.889984 10-11 AGAGTAC 11235 0.0 22.065441 10-11 GGGCTAT 1330 0.0 21.9074 6 CATGGGG 3750 0.0 21.815002 4 >>END_MODULE