FastQCFastQC Report
Mon 16 Jan 2017
ERR1146494_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146494_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3645524
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41350.040.7063181
GTATCAA67200.035.752461
CAACGCA74550.030.3840145
ATCAACG75850.029.8636683
AACGCAG84250.027.5551516
TATCAAC85700.027.4727822
TCAACGC86700.026.3970624
ACGCAGA92800.024.4070347
GTACATG120350.023.6745011
CGCAGAG96450.023.2410628
ACATGGG114500.023.0665443
TACATGG121100.022.624042
GTACTAG13100.020.457751
GCAGAGT107350.019.65559
CATGGGG89850.019.4567644
TGATCGC8900.019.0086969
AGAGTAC89550.018.8394710-11
CTGTGCG18800.018.4975289
TAGTACT19950.018.3737354
TCTAACG5500.017.9433022
Produced by FastQC (version 0.11.5)