##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146494_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3645524
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29998622968879	31.0	31.0	34.0	28.0	34.0
2	31.526229699763327	31.0	31.0	34.0	30.0	34.0
3	31.716903249025382	33.0	31.0	34.0	30.0	34.0
4	35.295712495652204	37.0	35.0	37.0	33.0	37.0
5	35.4073864278496	37.0	35.0	37.0	33.0	37.0
6	35.400248633666926	37.0	35.0	37.0	33.0	37.0
7	35.32710935382678	37.0	35.0	37.0	33.0	37.0
8	35.30545320782417	37.0	35.0	37.0	33.0	37.0
9	36.96551497123596	39.0	38.0	39.0	33.0	39.0
10-11	36.9452232381408	39.0	38.0	39.0	33.0	39.0
12-13	36.91142507908328	39.0	38.0	39.0	33.0	39.0
14-15	38.34802774580554	41.0	39.0	41.0	34.0	41.0
16-17	38.36790595810095	41.0	39.0	41.0	33.5	41.0
18-19	38.395677274378116	41.0	39.0	41.0	33.5	41.0
20-21	38.476115367777034	41.0	39.0	41.0	34.0	41.0
22-23	38.506568877341095	41.0	39.0	41.0	34.0	41.0
24-25	38.564458634753194	41.0	39.0	41.0	34.0	41.0
26-27	38.59472012802549	41.0	39.0	41.0	34.0	41.0
28-29	38.563131253559156	41.0	39.0	41.0	34.0	41.0
30-31	38.54651388936131	41.0	39.0	41.0	34.0	41.0
32-33	38.55601746141296	41.0	39.0	41.0	34.0	41.0
34-35	38.511266144455504	40.5	38.5	41.0	33.5	41.0
36-37	38.42310611588348	40.0	38.0	41.0	33.0	41.0
38-39	38.382176471750014	40.0	38.0	41.0	33.0	41.0
40-41	38.32155253949775	40.0	38.0	41.0	33.0	41.0
42-43	38.215911622032934	40.0	38.0	41.0	33.0	41.0
44-45	38.09616038736819	40.0	38.0	41.0	33.0	41.0
46-47	37.99974434402297	40.0	38.0	41.0	33.0	41.0
48-49	37.89375203674424	40.0	37.5	41.0	32.5	41.0
50-51	37.77421997496108	40.0	37.0	41.0	32.0	41.0
52-53	37.651446952482004	40.0	37.0	41.0	32.0	41.0
54-55	37.47412265013205	40.0	36.0	41.0	32.0	41.0
56-57	37.3479565626231	40.0	36.0	41.0	31.5	41.0
58-59	37.12647893691003	39.0	35.0	41.0	31.0	41.0
60-61	36.942515259809014	39.0	35.0	41.0	31.0	41.0
62-63	36.705951462670384	39.0	35.0	41.0	31.0	41.0
64-65	36.4474415749286	38.0	35.0	40.5	31.0	41.0
66-67	36.17797263164363	37.0	35.0	40.0	31.0	41.0
68-69	35.87822066183078	37.0	35.0	40.0	30.0	41.0
70-71	35.59804681028022	36.0	35.0	39.0	30.0	41.0
72-73	35.276239026268925	36.0	35.0	39.0	30.0	41.0
74-75	34.95866547579991	35.0	34.5	38.5	30.0	40.5
76-77	34.64531751814005	35.0	34.0	37.0	29.5	39.5
78-79	34.355387181650705	35.0	34.0	37.0	29.0	39.0
80-81	34.076325653047405	35.0	34.0	36.5	29.0	39.0
82-83	33.823738233515954	35.0	34.0	36.0	29.0	37.5
84-85	33.60115157656348	35.0	34.0	36.0	29.0	37.0
86-87	33.40881310889738	35.0	34.0	35.0	29.0	37.0
88-89	33.26339738813954	35.0	34.0	35.0	29.0	36.0
90-91	33.10805881404154	35.0	34.0	35.0	28.5	36.0
92-93	33.02089411563331	35.0	34.0	35.0	28.0	36.0
94-95	32.92678350217966	35.0	34.0	35.0	28.0	36.0
96-97	32.85481428732879	35.0	34.0	35.0	28.0	35.5
98-99	32.78705626406519	35.0	34.0	35.0	27.5	35.0
100	31.91695048503315	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.21067647829486447
1101	2	0.28278927037218793
1101	3	0.36973216972706524
1101	4	0.3395021810374317
1101	5	0.33479062634033596
1101	6	0.34712348960454875
1101	7	0.27167302873382226
1101	8	0.40963637610820314
1101	9	0.463655568197332
1101	10-11	0.556511175666742
1101	12-13	0.46293376036686595
1101	14-15	0.5653275385238459
1101	16-17	0.5453513215298713
1101	18-19	0.4953082258783965
1101	20-21	0.5332991966285263
1101	22-23	0.5373908436286854
1101	24-25	0.5313120568802887
1101	26-27	0.5187299480925773
1101	28-29	0.536747765074459
1101	30-31	0.49818511008304256
1101	32-33	0.5036374836917688
1101	34-35	0.4962754014789965
1101	36-37	0.5494132477098645
1101	38-39	0.5150806361716036
1101	40-41	0.5377859915047409
1101	42-43	0.5190108743148159
1101	44-45	0.5044660728754735
1101	46-47	0.47846875126036537
1101	48-49	0.43586494775261286
1101	50-51	0.4365696372059631
1101	52-53	0.39917627296181735
1101	54-55	0.4578439364787954
1101	56-57	0.420433596784477
1101	58-59	0.40495591343604076
1101	60-61	0.42538877905916195
1101	62-63	0.44096969226712446
1101	64-65	0.4042182413722344
1101	66-67	0.4147055145742371
1101	68-69	0.35346782106934427
1101	70-71	0.3636944874209078
1101	72-73	0.3590390364310352
1101	74-75	0.44137610898411594
1101	76-77	0.41852802226982533
1101	78-79	0.46202579073928973
1101	80-81	0.4763524409894231
1101	82-83	0.44037788511756304
1101	84-85	0.472287399353867
1101	86-87	0.475240292747074
1101	88-89	0.4445054578194316
1101	90-91	0.5038256137528592
1101	92-93	0.4676614926562195
1101	94-95	0.42837772948998776
1101	96-97	0.3833366783108687
1101	98-99	0.3564442313607472
1101	100	0.28742465137904105
1102	1	0.274035114346475
1102	2	0.278191363033816
1102	3	0.3563583927686125
1102	4	0.3340746096668141
1102	5	0.35497434261713323
1102	6	0.40923634571655754
1102	7	0.37929248625418666
1102	8	0.4178790479498389
1102	9	0.5661927084679022
1102	10-11	0.5719339754668269
1102	12-13	0.48848607911498476
1102	14-15	0.5803034090268824
1102	16-17	0.5948400805440244
1102	18-19	0.5232923923009736
1102	20-21	0.5801035868337934
1102	22-23	0.5684938296353508
1102	24-25	0.5039494064386432
1102	26-27	0.4716922500726213
1102	28-29	0.5231024563815012
1102	30-31	0.5291171358354632
1102	32-33	0.5252975847939183
1102	34-35	0.5413518542740121
1102	36-37	0.5866125997997855
1102	38-39	0.5396174870485879
1102	40-41	0.5277462097968808
1102	42-43	0.4752556807495125
1102	44-45	0.4861513193253799
1102	46-47	0.45749197311379675
1102	48-49	0.46409219758280074
1102	50-51	0.43681943288706293
1102	52-53	0.3732565811245365
1102	54-55	0.3974725999948703
1102	56-57	0.40562620885084044
1102	58-59	0.42509692343588057
1102	60-61	0.4395143982213412
1102	62-63	0.40663494929628996
1102	64-65	0.3125930729234483
1102	66-67	0.3531652514296866
1102	68-69	0.3483151375699691
1102	70-71	0.3281839027963045
1102	72-73	0.36289942708895495
1102	74-75	0.3265802175081518
1102	76-77	0.3090159964435202
1102	78-79	0.33383481421522276
1102	80-81	0.30608415907645536
1102	82-83	0.3385426279144994
1102	84-85	0.3438046867016098
1102	86-87	0.3131115208886044
1102	88-89	0.31217805402140186
1102	90-91	0.3718992119846334
1102	92-93	0.31461438177640133
1102	94-95	0.30944538821565715
1102	96-97	0.3251480245037115
1102	98-99	0.34785840016819947
1102	100	0.2741762002248862
1103	1	0.1724176239865649
1103	2	0.17809847811415835
1103	3	0.1902262266665531
1103	4	0.1810527063071916
1103	5	0.21541737039228082
1103	6	0.2479451688466341
1103	7	0.17631176855161357
1103	8	0.25247014540180857
1103	9	0.362049790811497
1103	10-11	0.31528236253251407
1103	12-13	0.29494105711930985
1103	14-15	0.3714815832061973
1103	16-17	0.3741931454125691
1103	18-19	0.3052048436772097
1103	20-21	0.3238742202820717
1103	22-23	0.30648102947533573
1103	24-25	0.2988868135686431
1103	26-27	0.33174247663463774
1103	28-29	0.3578340594618581
1103	30-31	0.314251180548176
1103	32-33	0.31776707997417475
1103	34-35	0.32270135468542094
1103	36-37	0.29209980439255645
1103	38-39	0.3092629747244757
1103	40-41	0.3074593369176526
1103	42-43	0.2592356826161435
1103	44-45	0.2472371492973835
1103	46-47	0.28581814654027937
1103	48-49	0.23905640689091712
1103	50-51	0.29795948441434206
1103	52-53	0.2849527727632548
1103	54-55	0.29426239566086565
1103	56-57	0.2337996078283311
1103	58-59	0.3165349490249696
1103	60-61	0.23417660796813067
1103	62-63	0.25701299034453484
1103	64-65	0.27806003462541184
1103	66-67	0.22082981754162745
1103	68-69	0.2899221611890255
1103	70-71	0.22161946922741294
1103	72-73	0.2261145451408666
1103	74-75	0.1857743235578937
1103	76-77	0.12184720209809541
1103	78-79	0.09647882861575852
1103	80-81	0.16647917265638057
1103	82-83	0.12864735766200397
1103	84-85	0.21311776228890977
1103	86-87	0.19365987394643724
1103	88-89	0.09393176233322009
1103	90-91	0.13402165782066788
1103	92-93	0.11387147484582272
1103	94-95	0.20221702355129167
1103	96-97	0.13821074611895767
1103	98-99	0.049086135519445406
1103	100	0.10472915353779655
1104	1	0.09585631619002655
1104	2	0.10675318054265404
1104	3	0.17270587105124235
1104	4	0.19489819351573345
1104	5	0.22730389456096844
1104	6	0.23885139097432528
1104	7	0.16839981368374168
1104	8	0.1985347677563709
1104	9	0.2583199400570919
1104	10-11	0.25956117410071755
1104	12-13	0.17128140677530723
1104	14-15	0.2361340937775367
1104	16-17	0.28351912330758466
1104	18-19	0.19443871498479837
1104	20-21	0.2788271184070652
1104	22-23	0.22966507507548783
1104	24-25	0.23314274970384474
1104	26-27	0.27309257338670534
1104	28-29	0.27867565378137016
1104	30-31	0.292664969565692
1104	32-33	0.24714258908146292
1104	34-35	0.2917037547714898
1104	36-37	0.32474297044371525
1104	38-39	0.26388221081192853
1104	40-41	0.2502788728547358
1104	42-43	0.16685858353240235
1104	44-45	0.11044408024620367
1104	46-47	0.09064444644641867
1104	48-49	0.16550611390788106
1104	50-51	0.14682248020366018
1104	52-53	0.14256708423700815
1104	54-55	0.11916016518220118
1104	56-57	0.1534524381797766
1104	58-59	0.19829464195673552
1104	60-61	0.2233058814326725
1104	62-63	0.235266765361132
1104	64-65	0.21789446041684357
1104	66-67	0.16804391305226574
1104	68-69	0.13096045993900418
1104	70-71	0.12800966739688846
1104	72-73	0.11243754982453424
1104	74-75	0.08554005364067052
1104	76-77	0.07708746851210435
1104	78-79	0.1316161555812556
1104	80-81	0.08693610125022389
1104	82-83	0.03604622658240686
1104	84-85	0.06738355459246748
1104	86-87	0.11959712713940718
1104	88-89	0.05715302141410916
1104	90-91	0.1530672901945067
1104	92-93	0.11980661848161844
1104	94-95	0.05549930609709719
1104	96-97	0.09356064257465135
1104	98-99	0.05951338405700568
1104	100	0.019067374796879477
1105	1	0.11338657360061433
1105	2	0.14931469306159073
1105	3	0.19628875211014218
1105	4	0.17548852009394977
1105	5	0.12003175092171148
1105	6	0.11921921040427463
1105	7	0.11559671675683347
1105	8	0.11865342301504
1105	9	0.1799582008758307
1105	10-11	0.26188784180845914
1105	12-13	0.19418336481994913
1105	14-15	0.33434899102608284
1105	16-17	0.25772121623548117
1105	18-19	0.2501709794801812
1105	20-21	0.2730578226584015
1105	22-23	0.27080914902475683
1105	24-25	0.2974593244544721
1105	26-27	0.32358247266717655
1105	28-29	0.31869143886677165
1105	30-31	0.29194911571448756
1105	32-33	0.27266527312131217
1105	34-35	0.2244717108198273
1105	36-37	0.23154532857200394
1105	38-39	0.2354891480304957
1105	40-41	0.23524137585695826
1105	42-43	0.22950041871180815
1105	44-45	0.17468930933313231
1105	46-47	0.13860265911996805
1105	48-49	0.16433399026824702
1105	50-51	0.13700006583900404
1105	52-53	0.06735619787044556
1105	54-55	0.15686617721301843
1105	56-57	0.12344924646016153
1105	58-59	0.11416041074107852
1105	60-61	0.08088589587988793
1105	62-63	0.09041463467140431
1105	64-65	0.12826455834190398
1105	66-67	0.1083578431047485
1105	68-69	0.11266560531977632
1105	70-71	0.11276635242849409
1105	72-73	0.09189433649802936
1105	74-75	0.1151736177458389
1105	76-77	0.12992812179638236
1105	78-79	0.1343552583016674
1105	80-81	0.15468183398046875
1105	82-83	0.1701566682999598
1105	84-85	0.17434588682171182
1105	86-87	0.13284196445302854
1105	88-89	0.15401155198109961
1105	90-91	0.160528790932851
1105	92-93	0.13738664662719913
1105	94-95	0.1243925054056021
1105	96-97	0.09122415770358572
1105	98-99	0.12259333868801292
1105	100	0.02490447746431812
1106	1	0.1071549560687508
1106	2	0.1502712541305513
1106	3	0.1546381663593266
1106	4	0.17673396629190563
1106	5	0.17328684161439156
1106	6	0.17058263246740069
1106	7	0.12170742791851552
1106	8	0.1620177091163697
1106	9	0.2598399706649417
1106	10-11	0.28055681749189887
1106	12-13	0.21395531623770125
1106	14-15	0.26243766036669314
1106	16-17	0.2276492400835295
1106	18-19	0.24373732298034412
1106	20-21	0.22218034836298983
1106	22-23	0.21986530524168302
1106	24-25	0.2082372795370091
1106	26-27	0.21944821204096598
1106	28-29	0.2705298239115379
1106	30-31	0.22940515353163704
1106	32-33	0.22376176119673374
1106	34-35	0.22480084892328023
1106	36-37	0.2261514668557183
1106	38-39	0.22210628731941995
1106	40-41	0.20418275949028697
1106	42-43	0.19449634733724963
1106	44-45	0.13916852910367794
1106	46-47	0.2126304041829883
1106	48-49	0.21228735505935248
1106	50-51	0.21745951373186045
1106	52-53	0.1771426469941204
1106	54-55	0.20072923895304484
1106	56-57	0.07988646011389022
1106	58-59	0.19629131547829104
1106	60-61	0.21053744593272938
1106	62-63	0.21114290755708254
1106	64-65	0.16113380289457524
1106	66-67	0.13486798394224309
1106	68-69	0.1503427764645835
1106	70-71	0.1719537989559896
1106	72-73	0.1734345140903244
1106	74-75	0.18649827604711078
1106	76-77	0.17573427423498345
1106	78-79	0.17868557892839476
1106	80-81	0.13223219351103666
1106	82-83	0.07103277871254221
1106	84-85	0.10118367286388263
1106	86-87	0.0980460142266466
1106	88-89	0.12420302638636826
1106	90-91	0.10243296673661462
1106	92-93	0.08742063311296988
1106	94-95	0.049864067895590836
1106	96-97	0.089351417556081
1106	98-99	0.08455350372521053
1106	100	0.062354598978906495
1107	1	0.08853778604637697
1107	2	0.15719863708037707
1107	3	0.16050066236444138
1107	4	0.19816952877192762
1107	5	0.20534256029448272
1107	6	0.22142105928107725
1107	7	0.15817799559189183
1107	8	0.21884497022215754
1107	9	0.26787768239393017
1107	10-11	0.3073593852052312
1107	12-13	0.2595131272396287
1107	14-15	0.36106229583329963
1107	16-17	0.32196526557090266
1107	18-19	0.2466086476847167
1107	20-21	0.2699495787480899
1107	22-23	0.2710244465408209
1107	24-25	0.2412196870553842
1107	26-27	0.27147811376675435
1107	28-29	0.32291489601923473
1107	30-31	0.2779693663392493
1107	32-33	0.28552820015487157
1107	34-35	0.3234037621942889
1107	36-37	0.30526407771381514
1107	38-39	0.26335885902763323
1107	40-41	0.21893100673381838
1107	42-43	0.2620304037450438
1107	44-45	0.24690612498095987
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2216	46-47	-0.22264871739982084
2216	48-49	-0.22776417608429256
2216	50-51	-0.22684975535330665
2216	52-53	-0.18743702242190352
2216	54-55	-0.16483195077464785
2216	56-57	-0.19068975238381114
2216	58-59	-0.12425829015613488
2216	60-61	-0.1518706160897878
2216	62-63	-0.120063709948937
2216	64-65	-0.11548497867852348
2216	66-67	-0.11737138280760462
2216	68-69	-0.11901506086640268
2216	70-71	-0.15240993354110088
2216	72-73	-0.14188759503552717
2216	74-75	-0.217334936424308
2216	76-77	-0.17158898275000922
2216	78-79	-0.17679703444957795
2216	80-81	-0.12875665883264276
2216	82-83	-0.12468191675696261
2216	84-85	-0.11761209873447598
2216	86-87	-0.11322866620852068
2216	88-89	-0.17479981181518411
2216	90-91	-0.1725215960375479
2216	92-93	-0.13676392336239473
2216	94-95	-0.17229859839390826
2216	96-97	-0.15965048814604188
2216	98-99	-0.22532350300335224
2216	100	-0.29430293645368977
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	100.0
1	50.0
2	54.0
3	49.0
4	31.0
5	52.0
6	117.0
7	601.0
8	1628.0
9	2020.0
10	2218.0
11	2748.0
12	3710.0
13	4638.0
14	5753.0
15	6954.0
16	7295.0
17	8156.0
18	9049.0
19	10373.0
20	12010.0
21	13464.0
22	14987.0
23	17447.0
24	21513.0
25	27173.0
26	30516.0
27	28763.0
28	30329.0
29	38584.0
30	44325.0
31	48282.0
32	61489.0
33	83773.0
34	125631.0
35	198234.0
36	372770.0
37	864704.0
38	1145636.0
39	399524.0
40	774.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.73724631236859	19.93522284986523	13.26152950928564	24.06600132848054
2	15.112956106109527	20.967623794400108	40.005135553088365	23.914284546401998
3	17.778666028735437	26.289835494947212	30.626871682547275	25.304626793770073
4	12.389534936355279	17.679951782598117	39.2769364940934	30.653576786953206
5	13.05019006316418	38.51854432685597	35.44705445990019	12.98421115007967
6	29.84629701060223	38.609803565461625	18.36392157981631	13.179977844119831
7	26.449976559634674	33.27333111868286	23.16763962241947	17.109052699262996
8	25.601066355618844	35.229841717295606	21.0580271159157	18.11106481116985
9	26.37007759100291	15.73324649929717	20.64649293275556	37.25018297694436
10-11	24.249109531469806	26.627971794697352	28.969607754921995	20.15331091891085
12-13	25.027590283940576	24.499486325561236	28.387748075949748	22.08517531454844
14-15	22.469852125731194	25.881241071202	25.68105767720599	25.967849125860816
16-17	22.28089506498402	28.578880712000593	26.6098068798248	22.53041734319059
18-19	22.29156807959937	28.29010590479953	27.726578799014064	21.691747216587043
20-21	23.26849079484312	27.510348008849284	27.15424235786327	22.066918838444323
22-23	23.313049960464223	27.2494415957503	26.948530798471786	22.48897764531369
24-25	22.898293540075212	27.518247053876095	27.190133382418253	22.39332602363044
26-27	22.712356421123463	27.6904112771938	26.872764525773253	22.72446777590949
28-29	22.58961828593249	27.860770626457015	26.999084298824734	22.55052678878576
30-31	22.867860313377527	27.632278499764624	26.855487288995793	22.644373897862057
32-33	22.777570346773125	27.871914265671837	26.444403354511415	22.90611203304362
34-35	23.190634727572085	27.712959113282317	26.79020933265315	22.306196826492453
36-37	23.18486698112625	27.38102995230978	26.78914449113859	22.644958575425374
38-39	22.92030593294486	27.492811091591946	26.92207445516977	22.664808520293423
40-41	22.915158546239812	27.267105395103204	27.086096648930692	22.731639409726295
42-43	22.881952232691912	27.349078340223578	26.841594420310884	22.92737500677363
44-45	22.743853033087202	27.595697761104958	26.947848565010325	22.712600640797515
46-47	22.972068406467255	27.548020995924865	26.634103656780646	22.845806940827234
48-49	22.97412395105442	27.415407968556465	26.706973543330076	22.90349453705904
50-51	22.89488050271494	27.50061293147529	26.655405465661225	22.949101100148546
52-53	22.95577310495951	27.449365251229686	26.7049698252184	22.889891818592407
54-55	22.68390600367435	27.518018432104846	26.762197857682857	23.03587770653795
56-57	22.968906417394052	27.453736125502733	26.668218949226365	22.90913850787685
58-59	22.85067857782327	27.326623589111747	26.846477384283666	22.97622044878132
60-61	22.685057126831552	27.56490363243008	26.847045090078076	22.902994150660287
62-63	22.799891118561952	27.337692432438214	26.750725990586478	23.111690458413356
64-65	22.515625951839855	27.49220829041193	26.713298015672876	23.27886774207534
66-67	22.79341739702043	27.383295810046782	26.582320337227987	23.2409664557048
68-69	22.730318754388954	27.367236438387515	26.763615398188467	23.13882940903507
70-71	22.785931760834995	27.159308415395316	26.770254342985023	23.284505480784667
72-73	22.869380936413183	27.299378252730467	26.81219928621723	23.019041524639118
74-75	22.879539688123778	27.683542719842112	26.646973852252575	22.789943739781535
76-77	23.03754701712415	27.37475752239685	26.516313401941904	23.07138205853709
78-79	22.871058580499	27.426635113174918	26.645129140092344	23.05717716623374
80-81	22.94298378492301	27.4922091450751	26.69269289706741	22.872114172934477
82-83	23.16370405881438	27.25089273390204	26.945786704857632	22.639616502425948
84-85	23.176171054420607	27.537541258030885	26.52900475414899	22.75728293339952
86-87	22.9376058233888	27.71302665764994	26.53141303970529	22.817954479255974
88-89	23.09053085280345	27.32419339813487	26.48245209546293	23.102823653598755
90-91	23.019871293126098	27.307370152655054	26.71067570079802	22.962082853420828
92-93	23.276085021679922	27.304449546212332	26.683282703630262	22.736182728477484
94-95	22.75241401793349	27.186089856240116	26.98077347362829	23.08072265219811
96-97	22.98393209918296	27.149076417779966	26.837426671757704	23.029564811279375
98-99	22.775428670209994	27.489977801619464	26.64938906434787	23.085204463822677
100	23.047154422467734	27.042555731009475	26.542791016088565	23.367498830434226
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1441.0
1	1047.5
2	740.5
3	824.5
4	769.5
5	670.5
6	624.5
7	647.5
8	690.5
9	683.0
10	599.0
11	529.0
12	517.0
13	532.0
14	578.0
15	700.5
16	818.5
17	939.0
18	1187.5
19	1474.5
20	1944.0
21	2826.5
22	4342.5
23	6019.0
24	8101.0
25	11500.0
26	15729.5
27	20559.0
28	26095.0
29	33603.0
30	42246.0
31	51387.5
32	63272.0
33	70693.5
34	74131.5
35	80083.0
36	87378.0
37	98689.0
38	105593.5
39	105207.5
40	105191.5
41	106393.5
42	109714.5
43	114256.5
44	119477.5
45	131131.5
46	141558.5
47	146035.5
48	148385.5
49	145330.5
50	142608.0
51	143518.5
52	140803.0
53	137184.0
54	133744.5
55	123065.0
56	112019.5
57	100154.0
58	86611.0
59	75740.0
60	62774.0
61	50070.5
62	40278.5
63	30299.0
64	21454.0
65	14806.5
66	10372.5
67	7601.0
68	5310.0
69	3811.0
70	2758.0
71	2008.0
72	1600.0
73	1278.0
74	920.5
75	640.0
76	459.5
77	333.5
78	251.5
79	187.5
80	156.5
81	113.0
82	84.5
83	68.0
84	50.5
85	39.5
86	33.0
87	24.5
88	20.5
89	15.0
90	12.5
91	13.5
92	10.0
93	8.0
94	5.0
95	3.5
96	6.0
97	5.5
98	5.0
99	7.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06210355493476384
2	0.009518521891503115
3	0.011191806719692424
4	0.010067139867958624
5	0.01152097750556573
6	0.012124457279666791
7	0.002825382578745881
8	0.00578791965160564
9	0.00702231009863054
10-11	0.006912586503339438
12-13	0.006007366842187845
14-15	0.010053424418547237
16-17	0.009833977227965033
18-19	0.0073789117833266225
20-21	0.007063456446864704
22-23	0.0025099272422839623
24-25	0.0038677567340113517
26-27	0.005061000832802088
28-29	0.0063091067292383755
30-31	0.009216782004452582
32-33	0.011054652225578545
34-35	0.012083310931432629
36-37	0.01773407608892439
38-39	0.018460994907727943
40-41	0.018447279458316555
42-43	0.024043182818162766
44-45	0.027513191519243873
46-47	0.03024256595211004
48-49	0.03154553364619188
50-51	0.031614110893248816
52-53	0.029899679716825344
54-55	0.03672997352369645
56-57	0.03917132351892348
58-59	0.03624993279429788
60-61	0.037086575208392536
62-63	0.032725062295571226
64-65	0.03472751790963384
66-67	0.03682598166957617
68-69	0.03930847801303736
70-71	0.03919875441774626
72-73	0.03505668869550715
74-75	0.03801922576836691
76-77	0.037662624083670826
78-79	0.03681226622016478
80-81	0.04136579542474553
82-83	0.037882071274253026
84-85	0.03383601369789364
86-87	0.03251933055440041
88-89	0.030571736737983347
90-91	0.03282107044145094
92-93	0.03438463167434915
94-95	0.03818381116130356
96-97	0.03315024122732425
98-99	0.03055802128857196
100	0.027677776912180527
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3645524.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.374055017999325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.65747083412825	37.87559151549573
2	14.902924853804953	16.206649118597998
3	6.276807557356104	10.238864383832242
4	3.1339713755878194	6.816269280041884
5	1.797587950297487	4.887107305458409
6	1.1441542779454557	3.7327384594851307
7	0.7695674419984958	2.929115170190105
8	0.5230329908490448	2.275153969652376
9	0.36487034996713413	1.7855532435194659
>10	1.397646287155771	11.898546242214797
>50	0.02702390209843538	0.9657085353669962
>100	0.004942178810998643	0.3887027761449164
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.7430898822775546E-5	0.0	0.0	0.0
6	0.0	2.7430898822775546E-5	0.0	0.0	5.486179764555109E-5
7	0.0	5.486179764555109E-5	0.0	0.0	5.486179764555109E-5
8	0.0	5.486179764555109E-5	0.0	0.0	5.486179764555109E-5
9	0.0	5.486179764555109E-5	0.0	0.0	5.486179764555109E-5
10-11	0.0	5.486179764555109E-5	0.0	4.114634823416332E-5	5.486179764555109E-5
12-13	0.0	5.486179764555109E-5	0.0	5.486179764555109E-5	9.600814587971442E-5
14-15	0.0	5.486179764555109E-5	0.0	5.486179764555109E-5	1.2343904470248996E-4
16-17	0.0	5.486179764555109E-5	0.0	1.2343904470248996E-4	1.3715449411387772E-4
18-19	0.0	5.486179764555109E-5	0.0	1.6458539293665326E-4	1.6458539293665326E-4
20-21	0.0	8.229269646832663E-5	0.0	2.468780894049799E-4	1.6458539293665326E-4
22-23	0.0	8.229269646832663E-5	0.0	4.937561788099598E-4	1.6458539293665326E-4
24-25	0.0	8.229269646832663E-5	0.0	0.001028658705854083	1.6458539293665326E-4
26-27	0.0	8.229269646832663E-5	0.0	0.001933878367005676	1.6458539293665326E-4
28-29	0.0	8.229269646832663E-5	0.0	0.006034797741010621	1.6458539293665326E-4
30-31	0.0	8.229269646832663E-5	0.0	0.016581978338367817	1.6458539293665326E-4
32-33	0.0	8.229269646832663E-5	0.0	0.038403258351885766	1.6458539293665326E-4
34-35	0.0	8.229269646832663E-5	0.0	0.07182780856743777	1.9201629175942883E-4
36-37	0.0	8.229269646832663E-5	0.0	0.12306872756838248	1.9201629175942883E-4
38-39	0.0	8.229269646832663E-5	0.0	0.19279807237587793	2.468780894049799E-4
40-41	0.0	8.229269646832663E-5	0.0	0.2666420520067897	2.468780894049799E-4
42-43	0.0	8.229269646832663E-5	0.0	0.3400197063577143	2.7430898822775545E-4
44-45	0.0	9.600814587971442E-5	0.0	0.43023993258582305	3.01739887050531E-4
46-47	0.0	1.0972359529110219E-4	0.0	0.5377004787240462	3.01739887050531E-4
48-49	0.0	1.0972359529110219E-4	0.0	0.6484527327210026	3.1545533646191873E-4
50-51	0.0	1.0972359529110219E-4	0.0	0.7643757111460521	3.2917078587330653E-4
52-53	0.0	1.0972359529110219E-4	0.0	0.8730843631807115	3.2917078587330653E-4
54-55	0.0	1.7830084234804103E-4	0.0	0.9870597477893439	3.2917078587330653E-4
56-57	0.0	1.9201629175942883E-4	0.0	1.1170273464116545	3.428862352846943E-4
58-59	0.0	1.9201629175942883E-4	0.0	1.2636180697205668	3.566016846960821E-4
60-61	0.0	1.9201629175942883E-4	0.0	1.4219355022762161	3.566016846960821E-4
62-63	0.0	1.9201629175942883E-4	0.0	1.5743552915849683	3.8403258351885766E-4
64-65	0.0	1.9201629175942883E-4	0.0	1.7250057879196516	3.8403258351885766E-4
66-67	0.0	1.9201629175942883E-4	0.0	1.8927868805691583	3.977480329302454E-4
68-69	0.0	1.9201629175942883E-4	0.0	2.081196009133392	4.114634823416332E-4
70-71	0.0	2.057317411708166E-4	0.0	2.276641163245668	4.114634823416332E-4
72-73	0.0	2.1944719058220437E-4	0.0	2.478217123244834	4.114634823416332E-4
74-75	0.0	2.1944719058220437E-4	0.0	2.6774340259452414	4.25178931753021E-4
76-77	0.0	2.468780894049799E-4	0.0	2.892396264569922	4.3889438116440874E-4
78-79	0.0	2.468780894049799E-4	0.0	3.129879271128101	4.3889438116440874E-4
80-81	0.0	2.468780894049799E-4	0.0	3.375454392838999	4.800407293985721E-4
82-83	0.0	2.468780894049799E-4	0.0	3.629930841217888	5.760488752782865E-4
84-85	0.0	2.468780894049799E-4	0.0	3.883323769093277	5.760488752782865E-4
86-87	0.0	2.468780894049799E-4	0.0	4.144507072234335	6.309106729238375E-4
88	0.0	2.468780894049799E-4	0.0	4.349937073518101	6.583415717466131E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4135	0.0	40.706318	1
GTATCAA	6720	0.0	35.75246	1
CAACGCA	7455	0.0	30.384014	5
ATCAACG	7585	0.0	29.863668	3
AACGCAG	8425	0.0	27.555151	6
TATCAAC	8570	0.0	27.472782	2
TCAACGC	8670	0.0	26.397062	4
ACGCAGA	9280	0.0	24.407034	7
GTACATG	12035	0.0	23.674501	1
CGCAGAG	9645	0.0	23.241062	8
ACATGGG	11450	0.0	23.066544	3
TACATGG	12110	0.0	22.62404	2
GTACTAG	1310	0.0	20.45775	1
GCAGAGT	10735	0.0	19.6555	9
CATGGGG	8985	0.0	19.456764	4
TGATCGC	890	0.0	19.008696	9
AGAGTAC	8955	0.0	18.83947	10-11
CTGTGCG	1880	0.0	18.497528	9
TAGTACT	1995	0.0	18.373735	4
TCTAACG	550	0.0	17.943302	2
>>END_MODULE