FastQCFastQC Report
Mon 16 Jan 2017
ERR1146494_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146494_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3645524
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129360.35484610717142445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115640.3172109139865764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64900.17802653335981328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74750.059.3066941
GTATCAA139950.044.690591
CAACGCA174650.035.2625475
ATCAACG174850.035.2490963
TATCAAC181450.034.3122372
TCAACGC183750.033.6953054
AACGCAG187200.033.2665526
ACGCAGA207000.029.6969137
CGCAGAG212400.028.919788
GCAGAGT229500.026.580679
GTGGTAT25350.026.3617611
GTACTAG13400.026.3402861
CAGAGTA217950.023.16986310-11
GAGTACT160700.023.045412-13
TGGTATC28000.022.689342
AGAGTAC210800.022.651510-11
GTACATG114050.022.1586361
TGATCGC7200.021.540489
GTACTTT172150.021.33515714-15
AGTACTT167500.021.16987812-13
Produced by FastQC (version 0.11.5)