FastQCFastQC Report
Mon 16 Jan 2017
ERR1146493_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146493_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3654631
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52750.035.294351
GTATCAA76600.032.468291
CAACGCA84600.028.7725185
ATCAACG85500.028.6360993
TCAACGC90950.027.1777464
AACGCAG90750.026.8219156
TATCAAC94800.026.2730082
GTACTAG14550.024.8805661
GTACATG120350.024.6891
ACGCAGA100900.023.8427167
TACATGG123000.023.3441662
CGCAGAG103600.023.1312568
ACATGGG125600.021.738463
TGATCGC8800.020.8243149
CATGGGG87900.020.101544
GTGATCG9400.019.9949788
GCAGAGT114900.019.6704319
CTGTGCG16500.019.0800579
ATTGCGT5300.017.7321246
GTATAAG20950.017.7286451
Produced by FastQC (version 0.11.5)