##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146493_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3654631
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.354494612451983	31.0	31.0	34.0	28.0	34.0
2	31.397827030964276	31.0	31.0	34.0	28.0	34.0
3	31.589167552072972	33.0	31.0	34.0	28.0	34.0
4	35.088885854686836	37.0	35.0	37.0	32.0	37.0
5	35.16000110544676	37.0	35.0	37.0	32.0	37.0
6	35.11415653180855	37.0	35.0	37.0	32.0	37.0
7	35.10029247822831	37.0	35.0	37.0	32.0	37.0
8	35.10479443752324	37.0	35.0	37.0	32.0	37.0
9	36.71052809435481	39.0	37.0	39.0	32.0	39.0
10-11	36.68850411984137	39.0	37.0	39.0	32.0	39.0
12-13	36.637846201162304	39.0	37.0	39.0	32.0	39.0
14-15	38.03073990233214	40.0	38.0	41.0	33.0	41.0
16-17	38.043907715991025	40.0	38.0	41.0	32.5	41.0
18-19	38.06493938786159	40.0	38.0	41.0	32.5	41.0
20-21	38.163777410086	40.0	38.5	41.0	33.0	41.0
22-23	38.18237039526015	40.0	38.0	41.0	33.0	41.0
24-25	38.240647961449454	40.0	38.0	41.0	33.0	41.0
26-27	38.27665091222616	40.0	38.0	41.0	33.0	41.0
28-29	38.24225318506848	40.0	38.0	41.0	33.0	41.0
30-31	38.226097381650845	40.0	38.0	41.0	33.0	41.0
32-33	38.237421096685274	40.0	38.0	41.0	33.0	41.0
34-35	38.17741367049095	40.0	38.0	41.0	33.0	41.0
36-37	38.08480993019542	40.0	38.0	41.0	32.0	41.0
38-39	38.04575769756235	40.0	38.0	41.0	32.0	41.0
40-41	37.98306792122105	40.0	38.0	41.0	32.0	41.0
42-43	37.84712027014492	40.0	38.0	41.0	32.0	41.0
44-45	37.69230669252245	40.0	37.5	41.0	31.5	41.0
46-47	37.58596107240375	40.0	37.0	41.0	31.0	41.0
48-49	37.4748257758444	40.0	37.0	41.0	31.0	41.0
50-51	37.35535831114003	40.0	37.0	41.0	31.0	41.0
52-53	37.24489640677814	40.0	36.0	41.0	31.0	41.0
54-55	37.06987955829193	40.0	36.0	41.0	30.0	41.0
56-57	36.94735884416238	39.0	35.0	41.0	30.0	41.0
58-59	36.740508275664496	39.0	35.0	41.0	30.0	41.0
60-61	36.56335058176872	39.0	35.0	41.0	30.0	41.0
62-63	36.33207689093646	38.5	35.0	40.5	30.0	41.0
64-65	36.083351917060845	38.0	35.0	40.0	30.0	41.0
66-67	35.819882089327216	37.0	35.0	40.0	29.5	41.0
68-69	35.526756052799854	37.0	35.0	40.0	29.0	41.0
70-71	35.251980569310554	36.0	34.5	39.0	29.0	41.0
72-73	34.92863068802295	36.0	34.0	39.0	29.0	41.0
74-75	34.60812185963508	35.0	34.0	38.5	29.0	40.0
76-77	34.296407079127825	35.0	34.0	37.0	29.0	39.5
78-79	34.012595799685386	35.0	34.0	37.0	28.5	39.0
80-81	33.73441395314602	35.0	34.0	36.5	28.0	39.0
82-83	33.4830981568317	35.0	34.0	36.0	28.0	37.5
84-85	33.26157893915966	35.0	34.0	36.0	28.0	37.0
86-87	33.07074654048521	35.0	33.5	35.0	27.0	37.0
88-89	32.9241882422603	35.0	33.0	35.0	27.0	36.0
90-91	32.77268471153449	35.0	33.0	35.0	27.0	36.0
92-93	32.68909022552482	35.0	33.0	35.0	27.0	36.0
94-95	32.593998819579866	35.0	33.0	35.0	27.0	36.0
96-97	32.523453393789964	35.0	33.0	35.0	27.0	35.0
98-99	32.45539440233501	35.0	33.0	35.0	26.5	35.0
100	31.542783389075396	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2812510632785141
1101	2	0.26293388744914026
1101	3	0.3308963614179312
1101	4	0.35590027706146543
1101	5	0.2821166468167746
1101	6	0.32513580168303946
1101	7	0.30926793091508387
1101	8	0.3799959921111409
1101	9	0.5059105365891696
1101	10-11	0.5516018530877247
1101	12-13	0.5016971983376379
1101	14-15	0.5919083978290658
1101	16-17	0.5215134134959314
1101	18-19	0.5939458587215611
1101	20-21	0.5674399661613236
1101	22-23	0.49348830034102065
1101	24-25	0.5008261372890743
1101	26-27	0.4733934513779232
1101	28-29	0.5129221596798814
1101	30-31	0.4699800613789904
1101	32-33	0.47978451373052167
1101	34-35	0.38222999710814065
1101	36-37	0.4771096855015884
1101	38-39	0.48845261060053247
1101	40-41	0.4896943587320379
1101	42-43	0.44153580712528395
1101	44-45	0.3919181499413824
1101	46-47	0.31689440777891775
1101	48-49	0.3590304387277641
1101	50-51	0.3931957763211642
1101	52-53	0.3696391089382445
1101	54-55	0.35142216047032804
1101	56-57	0.30556555411760655
1101	58-59	0.32080224620634823
1101	60-61	0.36049561411478237
1101	62-63	0.3523296156111826
1101	64-65	0.2975436436927552
1101	66-67	0.31088953279018483
1101	68-69	0.34748550559410063
1101	70-71	0.32491912410079493
1101	72-73	0.3193980913515446
1101	74-75	0.3107464865589904
1101	76-77	0.32705189235294796
1101	78-79	0.33448555684609005
1101	80-81	0.33794780935028257
1101	82-83	0.2727173428249756
1101	84-85	0.3323904077455495
1101	86-87	0.19091396915867875
1101	88-89	0.22383586091032726
1101	90-91	0.24842554524822447
1101	92-93	0.227688220841749
1101	94-95	0.22525712956742439
1101	96-97	0.1904302726993805
1101	98-99	0.2026435705935299
1101	100	0.23052049605590597
1102	1	0.20449957238555427
1102	2	0.24697408983008273
1102	3	0.2710117913406265
1102	4	0.2362088139263392
1102	5	0.20705970038107324
1102	6	0.2477357544707388
1102	7	0.22963739900665558
1102	8	0.2745110898631893
1102	9	0.33586145834978254
1102	10-11	0.34118982574380396
1102	12-13	0.3841320266275261
1102	14-15	0.38093068302280386
1102	16-17	0.39131137614758416
1102	18-19	0.38795680899760754
1102	20-21	0.3836498989352677
1102	22-23	0.40202525880395257
1102	24-25	0.3661635093520559
1102	26-27	0.38095256178486636
1102	28-29	0.49803687188117607
1102	30-31	0.3775403750444042
1102	32-33	0.40448918842090364
1102	34-35	0.3682434096966958
1102	36-37	0.405785737079313
1102	38-39	0.41023247588817213
1102	40-41	0.38911182119450416
1102	42-43	0.411674385512832
1102	44-45	0.340771732057469
1102	46-47	0.3222201857780007
1102	48-49	0.2974058009141345
1102	50-51	0.27977393604784595
1102	52-53	0.26517953889102586
1102	54-55	0.24054585807115103
1102	56-57	0.20000050148551907
1102	58-59	0.2332082154557895
1102	60-61	0.2516228039282282
1102	62-63	0.19719249740008138
1102	64-65	0.18005282890003826
1102	66-67	0.18461245774754076
1102	68-69	0.2744846605055642
1102	70-71	0.2656909983706228
1102	72-73	0.2301006031179611
1102	74-75	0.16791756616127174
1102	76-77	0.23237178329850394
1102	78-79	0.21120548671483164
1102	80-81	0.2140134117155199
1102	82-83	0.216853772905921
1102	84-85	0.18517592215347634
1102	86-87	0.2148032178614656
1102	88-89	0.19723212793013545
1102	90-91	0.2721703138034641
1102	92-93	0.25754318051053104
1102	94-95	0.20656985693592134
1102	96-97	0.22652856180398118
1102	98-99	0.19575460385430432
1102	100	0.1923891457757314
1103	1	0.10384848616516962
1103	2	0.05881561175965189
1103	3	0.10609100925626791
1103	4	0.05668060918220874
1103	5	0.1385205677672019
1103	6	0.10919904084644827
1103	7	0.05947434881519342
1103	8	0.18182243707178714
1103	9	0.17989608450852046
1103	10-11	0.22292075743282425
1103	12-13	0.16247049846501227
1103	14-15	0.2648595808793246
1103	16-17	0.24788994077631799
1103	18-19	0.15215071399574498
1103	20-21	0.19307218725327147
1103	22-23	0.13078655688736518
1103	24-25	0.1808340555716157
1103	26-27	0.22762824360982847
1103	28-29	0.26692402486354183
1103	30-31	0.2089136849462534
1103	32-33	0.23265092582689562
1103	34-35	0.27369818683729363
1103	36-37	0.28364402262444344
1103	38-39	0.2246488995318643
1103	40-41	0.19920596479055064
1103	42-43	0.2669002548937769
1103	44-45	0.0930837483482847
1103	46-47	0.09570969715984745
1103	48-49	0.029005196716909154
1103	50-51	0.09797289270551346
1103	52-53	0.0714311406064354
1103	54-55	0.059196467415155496
1103	56-57	0.06012329404660477
1103	58-59	0.039758796652201056
1103	60-61	0.0013720533847916272
1103	62-63	0.0724690484285162
1103	64-65	0.13363278706553672
1103	66-67	0.09177403791034067
1103	68-69	0.07812282616251309
1103	70-71	0.05790358673561258
1103	72-73	0.04902050918379075
1103	74-75	0.06414621853383551
1103	76-77	0.060463284818403906
1103	78-79	0.1386764410923007
1103	80-81	0.11683933462305163
1103	82-83	0.058957999233896885
1103	84-85	0.10656763447864392
1103	86-87	0.04437544093951118
1103	88-89	-0.002969164658637169
1103	90-91	0.05310704755346052
1103	92-93	0.013226053460869025
1103	94-95	-0.05410497726656871
1103	96-97	0.0778320253782212
1103	98-99	-0.01738739562603797
1103	100	-0.11690835515778986
1104	1	0.15728772992953566
1104	2	0.051217083811611275
1104	3	0.13877865732682437
1104	4	0.04468659968414812
1104	5	0.05762766276266973
1104	6	0.06450290451985552
1104	7	0.001557633324807739
1104	8	0.11320894987302665
1104	9	0.11114824790433175
1104	10-11	0.14010040986182304
1104	12-13	0.20166121333853937
1104	14-15	0.17090529991464365
1104	16-17	0.15566700780421172
1104	18-19	0.12520831285198852
1104	20-21	0.137448524013017
1104	22-23	0.1953707825162425
1104	24-25	0.21649799332912067
1104	26-27	0.2229867494424127
1104	28-29	0.25453976233066555
1104	30-31	0.23607604536092452
1104	32-33	0.21780735786360594
1104	34-35	0.23658525791250895
1104	36-37	0.23401315519194554
1104	38-39	0.20442885103139474
1104	40-41	0.22157337303899283
1104	42-43	0.1713428092429936
1104	44-45	0.12384180729232241
1104	46-47	0.11001814032971424
1104	48-49	0.15303726649240446
1104	50-51	0.14049889891184364
1104	52-53	0.14315076333039656
1104	54-55	0.13782090038658623
1104	56-57	0.11966767026130753
1104	58-59	0.18062010765996206
1104	60-61	0.13281608978640946
1104	62-63	0.16044345503513568
1104	64-65	0.14576221924905752
1104	66-67	0.09847902867086589
1104	68-69	0.10576062858336144
1104	70-71	0.07498172371005296
1104	72-73	0.08628327641788758
1104	74-75	0.08509872803930563
1104	76-77	0.05446989975886396
1104	78-79	0.09244054777987287
1104	80-81	0.10794323217001534
1104	82-83	0.13964323507602927
1104	84-85	0.09227760542684393
1104	86-87	0.07863043560593752
1104	88-89	0.08139535928653885
1104	90-91	0.11482130135194524
1104	92-93	0.13381548285638445
1104	94-95	0.08143812599593758
1104	96-97	0.06215171988988999
1104	98-99	0.010682286258806073
1104	100	-0.024724670563053053
1105	1	0.12848381700629474
1105	2	0.11070019545483945
1105	3	0.11639684873732037
1105	4	0.15396624507567225
1105	5	0.12465231643711405
1105	6	0.15531259960967247
1105	7	0.0979791407434405
1105	8	0.15264277492627798
1105	9	0.19163621192335256
1105	10-11	0.2767163943069946
1105	12-13	0.25439549933604155
1105	14-15	0.27584814673978286
1105	16-17	0.26085319303550136
1105	18-19	0.2366054248863989
1105	20-21	0.29084069766492604
1105	22-23	0.22761627568176124
1105	24-25	0.2722721090757858
1105	26-27	0.31283308450903036
1105	28-29	0.31844697185746895
1105	30-31	0.31760482946027935
1105	32-33	0.3462922549659737
1105	34-35	0.2627724678545036
1105	36-37	0.24945471629589377
1105	38-39	0.2507189618843526
1105	40-41	0.2710725489141481
1105	42-43	0.2622370447986597
1105	44-45	0.20634979069430415
1105	46-47	0.20644998861774155
1105	48-49	0.22714279190059727
1105	50-51	0.2210062713536587
1105	52-53	0.23317781280679384
1105	54-55	0.27706122770906205
1105	56-57	0.28552830860240164
1105	58-59	0.16155277922396039
1105	60-61	0.2018565570424542
1105	62-63	0.18176870072821316
1105	64-65	0.25335120966496305
1105	66-67	0.15676462519414258
1105	68-69	0.12552864738429292
1105	70-71	0.15830686730633658
1105	72-73	0.13407400947865256
1105	74-75	0.1391751276943083
1105	76-77	0.1692169132829875
1105	78-79	0.17053805392102106
1105	80-81	0.17091652228314302
1105	82-83	0.1507548465102957
1105	84-85	0.12453446197309859
1105	86-87	0.14387769447490228
1105	88-89	0.11873255377703629
1105	90-91	0.1485731080619601
1105	92-93	0.1631287607680605
1105	94-95	0.14579136268612558
1105	96-97	0.16980902563888378
1105	98-99	0.12785143621966455
1105	100	0.07043675561724427
1106	1	0.15443606545574795
1106	2	0.12814683960840867
1106	3	0.13193743738190378
1106	4	0.17921580058283126
1106	5	0.14570344957553516
1106	6	0.13108294941342535
1106	7	0.08208579691871165
1106	8	0.10388504495173834
1106	9	0.10686364455879982
1106	10-11	0.20245445758424552
1106	12-13	0.14247125811637318
1106	14-15	0.18491981004186897
1106	16-17	0.17601141832656708
1106	18-19	0.16436758626210235
1106	20-21	0.15095277021791986
1106	22-23	0.19635491006409467
1106	24-25	0.22927757013486172
1106	26-27	0.22666808054641052
1106	28-29	0.2034439248921629
1106	30-31	0.22281061601782426
1106	32-33	0.22207737092963242
1106	34-35	0.17871702366922904
1106	36-37	0.18520120545896646
1106	38-39	0.23237547579267215
1106	40-41	0.2255886405789198
1106	42-43	0.21135776295995612
1106	44-45	0.19602308687792913
1106	46-47	0.1761206646026494
1106	48-49	0.2063214950524923
1106	50-51	0.18885182035518255
1106	52-53	0.1649002705503122
1106	54-55	0.15971329432307613
1106	56-57	0.08374945590340133
1106	58-59	0.1776388272147713
1106	60-61	0.11568687544356493
1106	62-63	0.1018120398409934
1106	64-65	0.22880750766513813
1106	66-67	0.22363759428276353
1106	68-69	0.2071999238404416
1106	70-71	0.15134686962095856
1106	72-73	0.16229603126285497
1106	74-75	0.15663910980471485
1106	76-77	0.14775878966819533
1106	78-79	0.12624826124216781
1106	80-81	0.108684508851141
1106	82-83	0.10777883751074313
1106	84-85	0.20342680709538286
1106	86-87	0.1055814528707657
1106	88-89	0.06604295445974628
1106	90-91	0.11262697232590568
1106	92-93	0.09451471766597308
1106	94-95	0.07844902259726894
1106	96-97	0.04755324042322684
1106	98-99	0.12055591294875256
1106	100	0.06619784189514277
1107	1	0.04757704694070952
1107	2	0.06243946212008211
1107	3	0.09591079548545167
1107	4	0.12034795941835341
1107	5	0.15090001096879035
1107	6	0.10659992327249768
1107	7	0.16161081413957845
1107	8	0.19431560137703485
1107	9	0.13386203032010258
1107	10-11	0.2139659973035677
1107	12-13	0.16748479128132487
1107	14-15	0.18547277377020066
1107	16-17	0.1603204963318774
1107	18-19	0.13121702023487103
1107	20-21	0.14733346614961818
1107	22-23	0.13716705651450667
1107	24-25	0.11667158353582607
1107	26-27	0.20050141040078273
1107	28-29	0.15467104201492532
1107	30-31	0.13634066729773053
1107	32-33	0.11777909510355755
1107	34-35	0.12212659847040896
1107	36-37	0.18119156177432671
1107	38-39	0.19161478320076952
1107	40-41	0.09080303165607262
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2216	56-57	-0.2591972373946376
2216	58-59	-0.19704487701080353
2216	60-61	-0.19067322103622786
2216	62-63	-0.20870668693811467
2216	64-65	-0.24048268592679278
2216	66-67	-0.28558116366677666
2216	68-69	-0.2526136030765045
2216	70-71	-0.24936150512944266
2216	72-73	-0.2747731748586517
2216	74-75	-0.2777543425897804
2216	76-77	-0.24339740470963278
2216	78-79	-0.28929156112448595
2216	80-81	-0.30540923409492393
2216	82-83	-0.20676555551176534
2216	84-85	-0.24330497085384906
2216	86-87	-0.1608557979941807
2216	88-89	-0.25705624390398896
2216	90-91	-0.24673307800954802
2216	92-93	-0.25510602451640807
2216	94-95	-0.22948953891149415
2216	96-97	-0.21921701217561207
2216	98-99	-0.26005186977373285
2216	100	-0.20024193310453953
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	103.0
1	52.0
2	46.0
3	35.0
4	43.0
5	63.0
6	133.0
7	550.0
8	1816.0
9	2911.0
10	3565.0
11	4608.0
12	5878.0
13	7090.0
14	7939.0
15	8793.0
16	9606.0
17	10375.0
18	11361.0
19	12687.0
20	14098.0
21	15957.0
22	17375.0
23	20446.0
24	24511.0
25	29749.0
26	33430.0
27	31929.0
28	33688.0
29	42990.0
30	48933.0
31	53541.0
32	68316.0
33	92139.0
34	134907.0
35	209785.0
36	395564.0
37	863787.0
38	1102227.0
39	333141.0
40	464.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.00308862612949	19.500480912312423	13.264199507561125	24.232230953996968
2	15.060486756455665	20.76999762743376	40.04785085725717	24.121664758853406
3	18.10109386818631	26.06666652071541	30.311877768740963	25.52036184235732
4	12.189975954177479	17.623505414072785	39.3613449241477	30.825173707602033
5	13.139486134250491	38.330828442136465	35.3523796283931	13.177305795219945
6	29.816916717932102	38.24106832058813	18.532126461064905	13.40988850041487
7	26.337819822423032	32.959468363629995	23.12577711813947	17.576934695807502
8	25.55534606873351	35.17582618151708	20.984513876708096	18.284313873041317
9	26.21144584857649	15.890550855098809	20.485155729861184	37.41284756646352
10-11	24.160382553847942	26.600271591340547	28.993846438014582	20.24549941679693
12-13	24.958512460491235	24.42638833267363	28.32997278220386	22.285126424631276
14-15	22.514024085386627	25.719183339723156	25.803756120614658	25.963036454275557
16-17	22.281500029075634	28.467768804238887	26.55641186149052	22.69431930519496
18-19	22.197980128567625	28.257717566899537	27.73789751176785	21.806404792764987
20-21	23.294220891459382	27.38064762585485	27.2124794954874	22.11265198719837
22-23	23.27203015754507	27.27067130035327	26.820560116775248	22.636738425326406
24-25	22.815172608734972	27.44606058267719	27.201183967080528	22.537582841507312
26-27	22.69415244108002	27.606836928586752	26.884949519051855	22.814061111281376
28-29	22.588976218508147	27.81077969334374	26.98209769409215	22.61814639405596
30-31	22.917658938519395	27.549539478680153	26.824130318331985	22.708671264468467
32-33	22.66871105624621	27.784154712821053	26.628479855597426	22.91865437533531
34-35	23.10763077651431	27.71576132281297	26.822729844500103	22.35387805617262
36-37	23.087938575935446	27.390086187727363	26.76826468944161	22.75371054689558
38-39	22.829214024351863	27.474500058840757	26.96522648217125	22.73105943463613
40-41	22.930686300562705	27.21425261901289	27.12738779070229	22.727673289722116
42-43	22.818545071325143	27.270398391997432	26.935343117593263	22.975713419084165
44-45	22.811387437689667	27.56994324071888	26.9863524549501	22.63231686664135
46-47	22.87783064030073	27.391773700958698	26.725289872566105	23.00510578617447
48-49	22.909290621406637	27.394437073911497	26.81232767938793	22.883944625293935
50-51	22.962422526475496	27.500601832444648	26.617156966797324	22.91981867428253
52-53	22.847708040177487	27.51391888685035	26.73214562846638	22.906227444505785
54-55	22.71998843770671	27.415144496503054	26.79946059055015	23.065406475240092
56-57	22.973459408692587	27.406403608493363	26.63075984374072	22.989377139073333
58-59	22.829304304636533	27.29225156117477	26.90848457616673	22.96995955802197
60-61	22.719475750518892	27.504853591589345	26.869455665511747	22.906214992380015
62-63	22.815698743193987	27.410091673810722	26.771232620241815	23.002976962753475
64-65	22.671697355042774	27.377785162252156	26.783666485506775	23.166850997198292
66-67	22.883372834847357	27.321671433445776	26.69762187518434	23.097333856522525
68-69	22.745639933355715	27.430312090205472	26.749730675901862	23.07431730053695
70-71	22.797490546160837	27.176839794637253	26.728979176985966	23.296690482215944
72-73	22.914661350208625	27.2885584974391	26.717911526521366	23.07886862583091
74-75	22.940379266557155	27.575222222435126	26.608332564602794	22.87606594640493
76-77	22.995777125746503	27.300387961345656	26.5958821296075	23.107952783300334
78-79	22.846694199323203	27.324174790168993	26.690846133053213	23.138284877454588
80-81	22.93152132404973	27.509714525045613	26.69927881884515	22.859485332059506
82-83	23.161916164845383	27.225566785001504	26.915235521364462	22.69728152878865
84-85	23.185322066225282	27.469460889025626	26.542667164474885	22.802549880274203
86-87	22.877918450624268	27.684928042253315	26.64749545217406	22.789658054948358
88-89	23.12880389501332	27.24501654855136	26.537351115042814	23.088828441392508
90-91	23.079202300101496	27.207448177097305	26.636378018965328	23.076971503835875
92-93	23.199017333524942	27.249245550286382	26.75670793044692	22.795029185741754
94-95	22.847471879778773	27.243195243839065	26.93450684379729	22.97482603258487
96-97	22.849229668463124	27.163559807187536	26.804195385962288	23.183015138387052
98-99	22.825676241037158	27.4059723499731	26.593761876497968	23.174589532491773
100	23.066642361818175	27.108965642578593	26.606610699826362	23.217781295776867
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1474.0
1	1122.0
2	815.0
3	877.5
4	813.0
5	715.5
6	666.0
7	629.5
8	679.5
9	704.5
10	617.5
11	549.5
12	529.0
13	560.0
14	617.5
15	672.5
16	755.0
17	859.5
18	1101.5
19	1419.0
20	1889.5
21	2608.0
22	3814.0
23	5487.0
24	7558.0
25	10559.5
26	15277.0
27	21027.0
28	26542.5
29	33495.0
30	42256.0
31	51155.0
32	61118.0
33	69069.5
34	74976.5
35	81816.0
36	88646.5
37	98652.5
38	104796.5
39	104909.0
40	106021.0
41	108227.5
42	111541.5
43	116741.0
44	123242.5
45	132537.0
46	140173.0
47	145089.5
48	151069.0
49	149449.5
50	143417.5
51	141004.5
52	137907.0
53	134528.5
54	130000.0
55	120389.0
56	110005.5
57	97471.0
58	85277.0
59	76663.0
60	65190.5
61	52628.5
62	42451.5
63	32503.5
64	22826.0
65	15436.0
66	10808.5
67	7783.0
68	5594.5
69	4229.0
70	3325.0
71	2524.0
72	1812.5
73	1383.5
74	1013.5
75	734.0
76	590.5
77	445.5
78	317.5
79	250.0
80	204.5
81	161.0
82	125.0
83	98.5
84	76.0
85	53.5
86	42.0
87	28.0
88	24.5
89	23.0
90	15.5
91	15.0
92	11.5
93	7.5
94	8.0
95	9.0
96	6.5
97	3.5
98	4.5
99	8.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06014287078503958
2	0.009850515688177549
3	0.012121606805174037
4	0.009795790601021006
5	0.01223105697948712
6	0.01223105697948712
7	0.0028183419885619097
8	0.005417783628497651
9	0.00705953624319391
10-11	0.006662779361308981
12-13	0.006033440859008748
14-15	0.01045249164689951
16-17	0.01008309730859285
18-19	0.007921456365909445
20-21	0.006909042253513419
22-23	0.0024489476502552515
24-25	0.0037623497420122575
26-27	0.0050073454748235866
28-29	0.005841903053960851
30-31	0.009535846437027432
32-33	0.011013423790254065
34-35	0.012272100794854529
36-37	0.018839111253639563
38-39	0.019181143048367946
40-41	0.018551804546067717
42-43	0.024776783210124362
44-45	0.028552814223925756
46-47	0.03089231169986792
48-49	0.03267087703245553
50-51	0.03298554628360565
52-53	0.031248024766385444
54-55	0.037664541235489984
56-57	0.04027766414721486
58-59	0.0375550910611769
60-61	0.03854014262999466
62-63	0.03401164166779081
64-65	0.03613223879510681
66-67	0.03818442956347713
68-69	0.041166946813508666
70-71	0.040647058485521526
72-73	0.03615960133868508
74-75	0.038909536968301316
76-77	0.039183162404084024
78-79	0.03812970447632059
80-81	0.04367061955092046
82-83	0.03934733766555365
84-85	0.03510614341092165
86-87	0.033847466406321185
88-89	0.03116593713565063
90-91	0.03357384097053848
92-93	0.03573548191322188
94-95	0.03919684367587316
96-97	0.03383378513453205
98-99	0.03131643112533112
100	0.028429682777823536
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3654631.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.64624577965177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.61986976610243	41.286166152433914
2	14.679164587035814	16.92397459248454
3	5.858732672420622	10.132018307749059
4	2.892129641615709	6.668816645887815
5	1.555771212945725	4.484218485918811
6	0.9555071693236491	3.304884067623025
7	0.6185835490671804	2.4961313513343173
8	0.40670593502357677	1.8756056232329756
9	0.2923691410214092	1.5168585025535246
>10	1.0927042792101656	9.823357616838269
>50	0.022816110336323015	0.8752313456578816
>100	0.005489392830642247	0.5002065306957292
>500	7.827153332695206E-5	0.03293803834832449
>1k	7.827153332695203E-5	0.07959273924170464
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.4725087156541934E-5	0.0
3	0.0	0.0	0.0	5.4725087156541934E-5	0.0
4	0.0	0.0	0.0	1.0945017431308387E-4	0.0
5	0.0	0.0	0.0	1.0945017431308387E-4	0.0
6	0.0	0.0	0.0	1.0945017431308387E-4	0.0
7	0.0	0.0	0.0	1.0945017431308387E-4	0.0
8	0.0	0.0	0.0	1.0945017431308387E-4	0.0
9	0.0	0.0	0.0	1.9153780504789676E-4	0.0
10-11	0.0	0.0	0.0	4.2411942546319997E-4	0.0
12-13	0.0	0.0	0.0	4.378006972523355E-4	0.0
14-15	0.0	0.0	0.0	4.378006972523355E-4	0.0
16-17	0.0	0.0	0.0	4.378006972523355E-4	0.0
18-19	0.0	0.0	0.0	4.378006972523355E-4	0.0
20-21	0.0	0.0	0.0	4.925257844088774E-4	2.7362543578270967E-5
22-23	0.0	0.0	0.0	7.387886766133161E-4	2.7362543578270967E-5
24-25	0.0	0.0	0.0	0.001381808450702684	2.7362543578270967E-5
26-27	0.0	0.0	0.0	0.002339497475942168	2.7362543578270967E-5
28-29	0.0	0.0	0.0	0.0054314649002867865	2.7362543578270967E-5
30-31	0.0	0.0	0.0	0.013859128322394245	2.7362543578270967E-5
32-33	0.0	0.0	0.0	0.032219395063414064	2.7362543578270967E-5
34-35	0.0	0.0	0.0	0.062236105368777314	2.7362543578270967E-5
36-37	0.0	0.0	0.0	0.1086156167339466	2.7362543578270967E-5
38-39	0.0	0.0	0.0	0.17239770581489622	2.7362543578270967E-5
40-41	0.0	0.0	0.0	0.24300674951862444	2.7362543578270967E-5
42-43	0.0	0.0	0.0	0.3099902561982318	2.7362543578270967E-5
44-45	0.0	0.0	0.0	0.3882060870167193	2.7362543578270967E-5
46-47	0.0	0.0	0.0	0.4830446630590065	2.7362543578270967E-5
48-49	0.0	0.0	0.0	0.5829453096632737	2.7362543578270967E-5
50-51	0.0	0.0	0.0	0.6850075972102245	2.7362543578270967E-5
52-53	0.0	0.0	0.0	0.780078754872927	2.7362543578270967E-5
54-55	0.0	0.0	0.0	0.8818263731687275	2.7362543578270967E-5
56-57	0.0	0.0	0.0	1.0008944815495737	2.7362543578270967E-5
58-59	0.0	0.0	0.0	1.1331650172069356	2.7362543578270967E-5
60-61	0.0	0.0	0.0	1.2688285082680029	2.7362543578270967E-5
62-63	2.7362543578270967E-5	0.0	0.0	1.4075018791226803	2.7362543578270967E-5
64-65	2.7362543578270967E-5	0.0	0.0	1.5473928831665904	2.7362543578270967E-5
66-67	2.7362543578270967E-5	0.0	0.0	1.6961493513298607	2.7362543578270967E-5
68-69	2.7362543578270967E-5	0.0	0.0	1.8622263095781761	2.7362543578270967E-5
70-71	2.7362543578270967E-5	0.0	0.0	2.0397271297704203	2.7362543578270967E-5
72-73	2.7362543578270967E-5	0.0	0.0	2.2187739336748358	2.7362543578270967E-5
74-75	2.7362543578270967E-5	0.0	0.0	2.3931964677145245	4.104381536740645E-5
76-77	2.7362543578270967E-5	0.0	0.0	2.58332510176814	5.4725087156541934E-5
78-79	2.7362543578270967E-5	0.0	0.0	2.790787907178591	5.4725087156541934E-5
80-81	2.7362543578270967E-5	0.0	0.0	3.0115489087680807	5.4725087156541934E-5
82-83	2.7362543578270967E-5	0.0	0.0	3.239807247298017	8.20876307348129E-5
84-85	2.7362543578270967E-5	0.0	0.0	3.465137793665079	8.20876307348129E-5
86-87	2.7362543578270967E-5	0.0	0.0	3.702165827411851	9.576890252394838E-5
88	2.7362543578270967E-5	0.0	0.0	3.8866030524011865	1.0945017431308387E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5275	0.0	35.29435	1
GTATCAA	7660	0.0	32.46829	1
CAACGCA	8460	0.0	28.772518	5
ATCAACG	8550	0.0	28.636099	3
TCAACGC	9095	0.0	27.177746	4
AACGCAG	9075	0.0	26.821915	6
TATCAAC	9480	0.0	26.273008	2
GTACTAG	1455	0.0	24.880566	1
GTACATG	12035	0.0	24.689	1
ACGCAGA	10090	0.0	23.842716	7
TACATGG	12300	0.0	23.344166	2
CGCAGAG	10360	0.0	23.131256	8
ACATGGG	12560	0.0	21.73846	3
TGATCGC	880	0.0	20.824314	9
CATGGGG	8790	0.0	20.10154	4
GTGATCG	940	0.0	19.994978	8
GCAGAGT	11490	0.0	19.670431	9
CTGTGCG	1650	0.0	19.080057	9
ATTGCGT	530	0.0	17.732124	6
GTATAAG	2095	0.0	17.728645	1
>>END_MODULE