FastQCFastQC Report
Mon 16 Jan 2017
ERR1146493_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146493_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3654631
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142340.38947844529310893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121760.3331663306090273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68180.18655782211665145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85250.051.1230431
GTATCAA151400.042.277871
ATCAACG189650.033.4950263
CAACGCA190350.033.3224455
TATCAAC193300.033.08842
TCAACGC195250.032.6065944
AACGCAG198900.032.0668646
ACGCAGA218450.028.9787337
CGCAGAG222200.028.4262168
GCAGAGT240900.025.8099339
GTACATG118500.024.7042731
GAGTACT164950.023.34858112-13
TACATGG122000.023.339082
GTACTAG15100.023.0650751
ACATGGG121300.022.2499433
CAGAGTA235950.021.790210-11
GTACTTT176200.021.56442814-15
AGAGTAC224850.021.48642210-11
AGTACTT170350.021.03592312-13
CATGGGG84450.020.7119544
Produced by FastQC (version 0.11.5)