##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146493_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3654631
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.508648342336066	34.0	31.0	34.0	31.0	34.0
2	32.59763489118327	34.0	31.0	34.0	31.0	34.0
3	32.72299884721604	34.0	31.0	34.0	31.0	34.0
4	36.19304739657711	37.0	37.0	37.0	35.0	37.0
5	36.21293257787175	37.0	37.0	37.0	35.0	37.0
6	36.28670774149292	37.0	37.0	37.0	35.0	37.0
7	36.269700826157276	37.0	37.0	37.0	35.0	37.0
8	36.28061984917219	37.0	37.0	37.0	35.0	37.0
9	38.09395613401189	39.0	39.0	39.0	37.0	39.0
10-11	38.06060502414608	39.0	38.0	39.0	36.0	39.0
12-13	38.03635592758886	39.0	38.0	39.0	35.0	39.0
14-15	39.491956780315164	41.0	39.0	41.0	37.0	41.0
16-17	39.49084093578804	41.0	39.0	41.0	37.0	41.0
18-19	39.544374520984476	41.0	39.5	41.0	37.0	41.0
20-21	39.57886350222499	41.0	40.0	41.0	37.0	41.0
22-23	39.54041119335987	41.0	39.5	41.0	37.0	41.0
24-25	39.52573802936603	41.0	39.0	41.0	37.0	41.0
26-27	39.45106044905765	41.0	39.0	41.0	36.0	41.0
28-29	39.36168713065697	41.0	39.0	41.0	36.0	41.0
30-31	39.25462803221447	41.0	39.0	41.0	36.0	41.0
32-33	39.12516037870854	41.0	39.0	41.0	35.0	41.0
34-35	39.0421433791811	41.0	39.0	41.0	35.0	41.0
36-37	38.95129645099601	41.0	39.0	41.0	35.0	41.0
38-39	38.85376239078583	41.0	39.0	41.0	35.0	41.0
40-41	38.76357886746979	40.0	38.0	41.0	35.0	41.0
42-43	38.66472689034816	40.0	38.0	41.0	35.0	41.0
44-45	38.5155231814101	40.0	38.0	41.0	35.0	41.0
46-47	38.273800145623454	40.0	38.0	41.0	34.0	41.0
48-49	38.056559061639874	40.0	38.0	41.0	34.0	41.0
50-51	37.90711073156223	40.0	38.0	41.0	33.0	41.0
52-53	37.77745222431484	40.0	37.0	41.0	33.0	41.0
54-55	37.64112655969919	40.0	37.0	41.0	33.0	41.0
56-57	37.45072881502948	40.0	36.5	41.0	33.0	41.0
58-59	37.24865588345308	39.5	36.0	41.0	33.0	41.0
60-61	37.06648290894484	39.0	35.5	41.0	32.5	41.0
62-63	36.812505147578506	39.0	35.0	41.0	32.0	41.0
64-65	36.52006030157354	38.5	35.0	41.0	32.0	41.0
66-67	36.261735589721646	37.5	35.0	40.5	31.5	41.0
68-69	35.94606582716558	37.0	35.0	40.0	31.0	41.0
70-71	35.65668175528528	36.5	35.0	39.5	31.0	41.0
72-73	35.3282942929122	36.0	35.0	39.0	31.0	41.0
74-75	34.99598824067327	35.5	35.0	39.0	31.0	40.5
76-77	34.68299795519712	35.0	35.0	37.0	31.0	39.5
78-79	34.39267876839003	35.0	35.0	37.0	30.0	39.0
80-81	34.130046918553475	35.0	35.0	36.5	30.0	39.0
82-83	33.87628272183977	35.0	34.0	36.0	30.0	38.0
84-85	33.66389534264882	35.0	34.0	36.0	30.0	37.0
86-87	33.473536452790995	35.0	34.0	35.5	30.0	37.0
88-89	33.318892249313265	35.0	34.0	35.0	30.0	36.0
90-91	33.23243345224182	35.0	34.0	35.0	30.0	36.0
92-93	33.14649727975273	35.0	34.0	35.0	29.5	36.0
94-95	33.070684564324004	35.0	34.0	35.0	29.0	36.0
96-97	33.02393401686791	35.0	34.0	35.0	29.0	35.5
98-99	32.97003596259103	35.0	34.0	35.0	29.0	35.0
100	32.42199609208153	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.27141123361418806
1101	2	0.23221318723535234
1101	3	0.291065732945917
1101	4	0.2023180668893545
1101	5	0.17185917884139457
1101	6	0.1763632771944046
1101	7	0.1718231308858904
1101	8	0.17399129501483657
1101	9	0.17544455262351022
1101	10-11	0.1967798828159104
1101	12-13	0.251444104142692
1101	14-15	0.30379941782332764
1101	16-17	0.3405264261112677
1101	18-19	0.30862834880567647
1101	20-21	0.30074169321384403
1101	22-23	0.31806873526478796
1101	24-25	0.2934891038303391
1101	26-27	0.33223321383390214
1101	28-29	0.3595209294470081
1101	30-31	0.37917575225227296
1101	32-33	0.36060261457343046
1101	34-35	0.368829020788219
1101	36-37	0.4068090757965237
1101	38-39	0.4484913355807336
1101	40-41	0.4260498236199268
1101	42-43	0.42490492481874753
1101	44-45	0.4946344285260196
1101	46-47	0.4307896460383418
1101	48-49	0.38161833442431004
1101	50-51	0.3488141677342611
1101	52-53	0.4429379482786828
1101	54-55	0.43866700201860453
1101	56-57	0.43037161712938143
1101	58-59	0.4229939895484094
1101	60-61	0.3258581508198475
1101	62-63	0.33688857120089466
1101	64-65	0.4520764652255309
1101	66-67	0.3326775104896811
1101	68-69	0.41344587485745166
1101	70-71	0.42958834310516636
1101	72-73	0.45104363092640654
1101	74-75	0.39239228841644547
1101	76-77	0.38279624114144184
1101	78-79	0.33977303375208123
1101	80-81	0.3648751812171511
1101	82-83	0.3154119850049213
1101	84-85	0.33997041452396815
1101	86-87	0.3118025975431564
1101	88-89	0.33239511445601977
1101	90-91	0.38278385869778475
1101	92-93	0.32874433667412717
1101	94-95	0.27729419306825775
1101	96-97	0.325199349323988
1101	98-99	0.3154915031965402
1101	100	0.3303406579515311
1102	1	0.20604778203782814
1102	2	0.25930776054407545
1102	3	0.22310150501827763
1102	4	0.20172459306920842
1102	5	0.16212089602760926
1102	6	0.13628082101050154
1102	7	0.15364300942006537
1102	8	0.14422579984211126
1102	9	0.1847054537776529
1102	10-11	0.19403603011863169
1102	12-13	0.22154123696045502
1102	14-15	0.27628956436194585
1102	16-17	0.2943106717945412
1102	18-19	0.31953022713226886
1102	20-21	0.3066737575076033
1102	22-23	0.3172531711252873
1102	24-25	0.2997067191868368
1102	26-27	0.3284439300628179
1102	28-29	0.3778886255399172
1102	30-31	0.3688391380193039
1102	32-33	0.28553376924833884
1102	34-35	0.34815100157092616
1102	36-37	0.32349053437219055
1102	38-39	0.36049002411812126
1102	40-41	0.4251433911828215
1102	42-43	0.3685018271679539
1102	44-45	0.4171513119136492
1102	46-47	0.412501587830576
1102	48-49	0.38412233759841286
1102	50-51	0.3358292707227122
1102	52-53	0.31087484886934647
1102	54-55	0.3116480289943695
1102	56-57	0.26028194005766636
1102	58-59	0.3149506701040963
1102	60-61	0.3821811649342024
1102	62-63	0.3234317294754163
1102	64-65	0.33570087049832154
1102	66-67	0.3216324221217093
1102	68-69	0.3525410084389691
1102	70-71	0.2884985768586219
1102	72-73	0.25048236878134844
1102	74-75	0.2636932991669312
1102	76-77	0.2371524980788422
1102	78-79	0.15305082615960686
1102	80-81	0.18806383489970813
1102	82-83	0.23652996899971157
1102	84-85	0.22018400538194527
1102	86-87	0.23221355576963987
1102	88-89	0.18107602452408855
1102	90-91	0.25701799024753313
1102	92-93	0.23144232739736026
1102	94-95	0.29857023174883324
1102	96-97	0.2098332661767799
1102	98-99	0.17433052187419662
1102	100	0.1719264207120048
1103	1	0.22740361771265327
1103	2	0.20685038303404468
1103	3	0.22106368820695366
1103	4	0.18142788616058425
1103	5	0.14703420107372978
1103	6	0.11721756807115469
1103	7	0.130397859467557
1103	8	0.1385962804312726
1103	9	0.13537217558000947
1103	10-11	0.19430372956193054
1103	12-13	0.17920068883835683
1103	14-15	0.19069455859044382
1103	16-17	0.2533011669224834
1103	18-19	0.2669106814834663
1103	20-21	0.2870162504050384
1103	22-23	0.30600349413050765
1103	24-25	0.28106561365002847
1103	26-27	0.30708798346587685
1103	28-29	0.3165122666108502
1103	30-31	0.2996254658120918
1103	32-33	0.2765721937354044
1103	34-35	0.2966409433306083
1103	36-37	0.26816899244469283
1103	38-39	0.3021315011109209
1103	40-41	0.28921549925681234
1103	42-43	0.2561755445178022
1103	44-45	0.22748380496016551
1103	46-47	0.3291159953893299
1103	48-49	0.27354298959330237
1103	50-51	0.32482058312483275
1103	52-53	0.26530065474959486
1103	54-55	0.2963252695856795
1103	56-57	0.24499296752659205
1103	58-59	0.2676944529636671
1103	60-61	0.23323004005967363
1103	62-63	0.23840724053356155
1103	64-65	0.20902126557983536
1103	66-67	0.24807089122608517
1103	68-69	0.2009914219921285
1103	70-71	0.21132657534085553
1103	72-73	0.22836236844059954
1103	74-75	0.1484646727057779
1103	76-77	0.1406361891552379
1103	78-79	0.1046414626644463
1103	80-81	0.12730735314346475
1103	82-83	0.1322469376348394
1103	84-85	0.15838838302544644
1103	86-87	0.0767833589707223
1103	88-89	0.08189691919882591
1103	90-91	0.1442918333564549
1103	92-93	0.12645174601637166
1103	94-95	0.09327040393545616
1103	96-97	0.09847477901961099
1103	98-99	0.08897752598309694
1103	100	0.11244328051922281
1104	1	0.13833513891157878
1104	2	0.08581596332246022
1104	3	0.10751154548546538
1104	4	0.07996268636046011
1104	5	0.09800563313737598
1104	6	0.0614813366965663
1104	7	0.06968821681677184
1104	8	0.046413058820078845
1104	9	0.10115996480161726
1104	10-11	0.10481686095362619
1104	12-13	0.12540357984226347
1104	14-15	0.18825880218645352
1104	16-17	0.18649272627799007
1104	18-19	0.17278819018081748
1104	20-21	0.18547333250430853
1104	22-23	0.232235106107872
1104	24-25	0.2037219194184985
1104	26-27	0.16048144657602137
1104	28-29	0.20159912063937924
1104	30-31	0.2049758467044427
1104	32-33	0.19047118688931164
1104	34-35	0.2372197417822477
1104	36-37	0.19833859510043794
1104	38-39	0.23237452874716524
1104	40-41	0.2410728082348541
1104	42-43	0.16499840718375225
1104	44-45	0.17069298126025245
1104	46-47	0.15942345770751842
1104	48-49	0.07965771001185118
1104	50-51	0.07963483328679644
1104	52-53	0.12086184888347873
1104	54-55	0.1064254785848675
1104	56-57	0.12629055381662369
1104	58-59	0.07248327270244914
1104	60-61	0.07726917328042049
1104	62-63	0.09075246681970839
1104	64-65	0.045447476273373866
1104	66-67	0.04088131663892369
1104	68-69	0.04515130973798165
1104	70-71	0.060049839304106456
1104	72-73	0.04101481629349735
1104	74-75	-0.010587956064256332
1104	76-77	-0.0112828102750413
1104	78-79	0.006761460085208171
1104	80-81	0.03635608864281892
1104	82-83	0.11188567915466763
1104	84-85	0.0828598788966417
1104	86-87	0.059096461775290265
1104	88-89	0.03891066526431075
1104	90-91	-2.8890662257197164E-5
1104	92-93	-4.208540453092269E-4
1104	94-95	-1.3860868732962217E-4
1104	96-97	-0.028210523155436817
1104	98-99	0.004205513388598092
1104	100	-0.018460390957081074
1105	1	0.11782305983078345
1105	2	0.11346173745865684
1105	3	0.14154809266454293
1105	4	0.09348927665209317
1105	5	0.08440407154262886
1105	6	0.09612174434597875
1105	7	0.09400727924872854
1105	8	0.10745100506476746
1105	9	0.11312546966775017
1105	10-11	0.10852249992617402
1105	12-13	0.13164298262156393
1105	14-15	0.20119166065780547
1105	16-17	0.14435923977631404
1105	18-19	0.14269811233375407
1105	20-21	0.14038258886731114
1105	22-23	0.17155121888400515
1105	24-25	0.18141101137943139
1105	26-27	0.1590551975986756
1105	28-29	0.21300928609213798
1105	30-31	0.18820260927572008
1105	32-33	0.16104576070561905
1105	34-35	0.19939264860411043
1105	36-37	0.20132380730993304
1105	38-39	0.1835511171114561
1105	40-41	0.13366621069072693
1105	42-43	0.09697105573854259
1105	44-45	0.12725567704465135
1105	46-47	0.08425276487351141
1105	48-49	0.08909058933857494
1105	50-51	0.09519315361011849
1105	52-53	0.07357696954579751
1105	54-55	0.02658358739111577
1105	56-57	0.06490643925182837
1105	58-59	0.06973721239040742
1105	60-61	0.07724773912762117
1105	62-63	0.14424449159781716
1105	64-65	0.1273194576221499
1105	66-67	0.0696550172459851
1105	68-69	0.08426279291460048
1105	70-71	0.0449586716142889
1105	72-73	0.03400941747221253
1105	74-75	0.0648062037291055
1105	76-77	-0.017031484782009443
1105	78-79	0.02206189533163183
1105	80-81	0.08948010580202492
1105	82-83	0.01986671971082643
1105	84-85	0.003954087187914013
1105	86-87	0.037862007372098105
1105	88-89	-0.023396622698768965
1105	90-91	-0.07815780526492233
1105	92-93	-0.02289231565735861
1105	94-95	0.007051806808419769
1105	96-97	-0.06532837931725766
1105	98-99	-0.038213041557469296
1105	100	-0.04968105766302955
1106	1	0.11030347958849518
1106	2	0.15616768972010675
1106	3	0.16216978628456502
1106	4	0.06367333676307396
1106	5	0.11672982516672192
1106	6	0.06825307373418354
1106	7	0.06586748633689865
1106	8	0.09925957899505988
1106	9	0.1331081150953679
1106	10-11	0.11679519766855861
1106	12-13	0.09502940778879321
1106	14-15	0.12493889304129624
1106	16-17	0.1519676951077571
1106	18-19	0.12434212846483916
1106	20-21	0.07021811703547343
1106	22-23	0.15018012820046067
1106	24-25	0.1558878954220546
1106	26-27	0.11011485920582231
1106	28-29	0.14132929622905976
1106	30-31	0.16606319425910954
1106	32-33	0.10420442508044658
1106	34-35	0.07056787275048038
1106	36-37	0.09882227606131266
1106	38-39	0.14345420226722894
1106	40-41	0.1498145308431802
1106	42-43	0.12740019942804537
1106	44-45	0.10270610542402636
1106	46-47	0.054383926155772144
1106	48-49	0.06762453722060258
1106	50-51	0.09559844641155024
1106	52-53	0.13926584648433504
1106	54-55	0.14880379367451724
1106	56-57	0.13730387738088012
1106	58-59	0.05546782106864612
1106	60-61	0.054060298801339
1106	62-63	0.046992297687076245
1106	64-65	0.06518827524537585
1106	66-67	0.0646992693105446
1106	68-69	0.06534186255839103
1106	70-71	0.026323673062911723
1106	72-73	0.004627865643442419
1106	74-75	0.009351055813397124
1106	76-77	-0.018746190652791483
1106	78-79	0.013530916213497335
1106	80-81	0.06671105204667072
1106	82-83	0.06577988696074044
1106	84-85	0.07428081339914883
1106	86-87	0.04164688334954292
1106	88-89	0.01432593119817227
1106	90-91	-0.020773629047425857
1106	92-93	-0.0368649028305299
1106	94-95	-0.06577716584214244
1106	96-97	-0.053973733324561124
1106	98-99	0.07087703971167514
1106	100	0.018286189347193726
1107	1	0.024877828500621035
1107	2	-0.0017743994665764262
1107	3	0.01292952216171983
1107	4	9.189376147276107E-4
1107	5	-0.027608789232012043
1107	6	-0.02014820374092352
1107	7	0.01906520970370451
1107	8	0.007029042051954093
1107	9	0.0018157500269353477
1107	10-11	0.0032524307799306484
1107	12-13	-0.0015687928916960914
1107	14-15	-0.02119116102178964
1107	16-17	0.010101440350275936
1107	18-19	0.017154591500862182
1107	20-21	-0.012227628541019442
1107	22-23	0.0024013937079274683
1107	24-25	0.007844603210060086
1107	26-27	0.016719322019191907
1107	28-29	0.052667809737876325
1107	30-31	0.08075304473329226
1107	32-33	0.027846429380446125
1107	34-35	0.039319603534430314
1107	36-37	0.04266485995650271
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2216	26-27	-0.4006510224330242
2216	28-29	-0.3569114738844519
2216	30-31	-0.4026080210210523
2216	32-33	-0.45296396638971004
2216	34-35	-0.4319722218809048
2216	36-37	-0.4776731127257676
2216	38-39	-0.4121782663615221
2216	40-41	-0.39437197273984026
2216	42-43	-0.4919836172135419
2216	44-45	-0.46483417917479386
2216	46-47	-0.48381170138553387
2216	48-49	-0.49234067441823015
2216	50-51	-0.4196791814592089
2216	52-53	-0.41704157840260336
2216	54-55	-0.4786947348995483
2216	56-57	-0.43884223426186253
2216	58-59	-0.37555458365085315
2216	60-61	-0.4030211546322491
2216	62-63	-0.3870305538831502
2216	64-65	-0.33023541588887184
2216	66-67	-0.337869037785147
2216	68-69	-0.37417670080928644
2216	70-71	-0.37657458577054825
2216	72-73	-0.307253775575262
2216	74-75	-0.2952873967237366
2216	76-77	-0.3348929096654558
2216	78-79	-0.33891255795822417
2216	80-81	-0.335266265710473
2216	82-83	-0.26535117387130214
2216	84-85	-0.25398764200262036
2216	86-87	-0.24681653253833957
2216	88-89	-0.25653951966816635
2216	90-91	-0.2752732870403136
2216	92-93	-0.30259966859280496
2216	94-95	-0.2923927205332433
2216	96-97	-0.2287581794485547
2216	98-99	-0.2678915269755038
2216	100	-0.3579155321389891
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	21.0
8	77.0
9	170.0
10	286.0
11	292.0
12	250.0
13	289.0
14	388.0
15	687.0
16	1372.0
17	2544.0
18	4509.0
19	7059.0
20	10462.0
21	15109.0
22	22346.0
23	27451.0
24	26967.0
25	23201.0
26	20504.0
27	21191.0
28	24144.0
29	29187.0
30	35512.0
31	45704.0
32	59274.0
33	78120.0
34	114132.0
35	183107.0
36	332301.0
37	834564.0
38	1263311.0
39	468879.0
40	1221.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.29952115297629	19.28815732692864	13.627944153942517	23.784377366152558
2	15.348406777024373	20.89210955725639	39.81485386760471	23.944629798114523
3	17.859545884014658	26.279771587556233	30.92282994360285	24.937852584826263
4	12.199042803500545	17.939704446221793	38.926611195494154	30.934641554783504
5	12.689142706355042	38.060420552535675	35.27562456305359	13.974812178055696
6	29.504969134966352	38.584507310110745	18.018864078102943	13.891659476819962
7	25.575079050453287	33.66966487423617	22.896557460841432	17.858698614469105
8	25.662125670142892	34.52991560570684	20.724007430572332	19.08395129357793
9	26.371526974953145	14.814053730732324	20.669528606307995	38.144890688006534
10-11	24.142902525590134	26.20104464718873	28.81939380473706	20.836659022484074
12-13	25.57934029454684	24.15848549415796	28.5460830382055	21.716091173089705
14-15	22.717022341115563	25.029746517396635	26.95984640850735	25.293384732980456
16-17	21.5142376891128	27.916375141566963	27.6455817290446	22.92380544027564
18-19	21.388493120098858	26.721316050785976	30.354227280401226	21.535963548713948
20-21	22.395899887020057	25.885308256839064	30.19597600961629	21.522815846524587
22-23	22.29361756969483	25.810024651050412	29.904044507062448	21.992313272192305
24-25	21.94909691293047	25.961157227638033	30.251508291808392	21.838237567623107
26-27	21.82641694879729	26.199115587866462	29.903607231482464	22.070860231853775
28-29	21.66029119226844	26.382164541964574	30.013612869155015	21.94393139661197
30-31	22.081615135437158	26.03586110051952	29.88227120759333	22.000252556449993
32-33	21.9930262523864	26.253508285395544	29.621653806487945	22.13181165573011
34-35	22.29227748780638	26.26490084346333	29.74672841575286	21.696093252977434
36-37	22.314266114704303	26.042091645125304	29.55454459137355	22.089097648796844
38-39	22.176231502089692	26.069883943586742	29.73349765583229	22.020386898491275
40-41	22.21439640734899	25.88552447256812	29.782848686911723	22.117230433171166
42-43	22.2681597432716	25.95386938904283	29.414328757868674	22.363642109816897
44-45	22.31943280389229	26.331818260175027	29.18294405776377	22.16580487816891
46-47	22.51262341062412	26.32500492185436	28.558056059750147	22.604315607771372
48-49	22.653301234990842	26.504527716013946	28.30907442178382	22.533096627211393
50-51	22.75193718376297	26.77259992997859	27.9481058989686	22.527356987289842
52-53	22.588647509387215	26.84456253091162	28.000881088006874	22.565908871694297
54-55	22.430451500203034	26.839914496143464	27.940152773019662	22.78948123063385
56-57	22.72509241095945	26.800628468403787	27.72129035223837	22.752988768398392
58-59	22.511983082899647	26.660413235420073	28.05754342490834	22.77006025677194
60-61	22.485390870155783	26.84253169461528	27.996732305139542	22.67534513008939
62-63	22.44128923371558	26.757451144649764	27.98229459149926	22.818965030135395
64-65	22.34345752803387	26.70857158649963	27.964104717422956	22.983866168043544
66-67	22.470299338015096	26.654305528799842	27.92138600900159	22.954009124183465
68-69	22.415521728066025	26.72338684741236	27.941860560570696	22.919230863950922
70-71	22.44722277024396	26.4567763169236	27.90614407921722	23.18985683361522
72-73	22.55560493229883	26.558596360265124	27.829652242394324	23.05614646504172
74-75	22.653759274537247	26.901856480854402	27.53052063280973	22.91386361179862
76-77	22.68489761339637	26.622911263484127	27.480652524762743	23.211538598356757
78-79	22.469261299815713	26.738653667506252	27.578637304544007	23.213447728134035
80-81	22.527488088050536	26.893622703374636	27.54587778210562	23.0330114264692
82-83	22.853565393653984	26.58346979286107	27.723915275016626	22.839049538468316
84-85	22.898076083256626	26.780947335816396	27.331591614796363	22.989384966130615
86-87	22.54999205118109	26.96056045056259	27.44163501048396	23.04781248777236
88-89	22.739154936206152	26.612317246906553	27.319284736170495	23.329243080716797
90-91	22.622960463849964	26.60667427345586	27.42061439872162	23.34975086397255
92-93	22.73691926763605	26.614643174646087	27.471268645179226	23.17716891253864
94-95	22.420228255633504	26.584096531783448	27.62989309042323	23.36578212215982
96-97	22.45978893852922	26.487619446331323	27.53131137347291	23.52128024166655
98-99	22.440104016670468	26.68343363368943	27.336280937496284	23.540181412143824
100	22.593653050424585	26.39625174369002	27.363797903248667	23.646297302636725
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	534.0
1	362.5
2	459.5
3	702.0
4	442.0
5	336.5
6	791.5
7	1990.0
8	3882.5
9	4736.0
10	3403.0
11	2038.0
12	1896.5
13	2113.0
14	2456.0
15	2830.0
16	3298.0
17	3864.5
18	4434.0
19	5034.5
20	5817.5
21	6879.0
22	8203.5
23	9744.5
24	11778.5
25	14571.0
26	18826.0
27	24488.5
28	29982.5
29	36547.5
30	44746.0
31	52643.0
32	61631.5
33	69158.0
34	74372.0
35	80242.0
36	86283.5
37	96361.0
38	102107.5
39	102061.5
40	103321.0
41	104995.5
42	108702.0
43	113859.0
44	120393.0
45	129933.0
46	136647.0
47	141353.0
48	147881.5
49	146550.5
50	140962.0
51	138480.0
52	135474.0
53	132197.5
54	127711.0
55	118275.0
56	107965.0
57	95800.0
58	83985.0
59	75813.0
60	64598.0
61	51841.5
62	41309.5
63	31407.0
64	21944.0
65	14579.0
66	10029.0
67	7102.0
68	5080.0
69	3784.5
70	2901.0
71	2248.5
72	1627.5
73	1183.5
74	826.5
75	607.5
76	451.0
77	308.0
78	226.0
79	153.0
80	103.5
81	70.0
82	51.0
83	38.5
84	26.5
85	14.5
86	10.0
87	10.5
88	7.0
89	5.0
90	2.5
91	1.0
92	1.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00623865993584578
2	0.09174660861794255
3	3.830756100957935E-4
4	0.0
5	0.039319975121975374
6	0.004706357495462606
7	8.20876307348129E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	4.104381536740645E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.472508715654193E-4
24-25	0.0
26-27	0.0
28-29	2.7362543578270964E-5
30-31	6.840635894567742E-5
32-33	0.001860652963322426
34-35	0.001532302440383174
36-37	9.30326481661213E-4
38-39	7.93513763769858E-4
40-41	0.0016280713429071224
42-43	8.071950355589935E-4
44-45	6.019759587219612E-4
46-47	3.4203179472838707E-4
48-49	7.93513763769858E-4
50-51	9.440077534503484E-4
52-53	0.0016007087993288515
54-55	0.001614390071117987
56-57	8.756013945046708E-4
58-59	0.0028457045321401806
60-61	0.0018332904197441546
62-63	0.0011355455584982452
64-65	0.0045832260493603874
66-67	0.004569544777571251
68-69	0.003858118644536206
70-71	0.0019701031376355097
72-73	0.005171520736293213
74-75	0.003721305926644852
76-77	0.0040770189931623735
78-79	9.850515688177549E-4
80-81	6.840635894567742E-5
82-83	1.7785653325876129E-4
84-85	8.20876307348129E-5
86-87	0.0
88-89	2.0521907683703224E-4
90-91	2.7362543578270964E-5
92-93	0.0
94-95	1.3681271789135482E-5
96-97	2.3258162041530324E-4
98-99	6.703823176676387E-4
100	6.840635894567741E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3654631.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.968141179670255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.80924142545621	36.4469761428958
2	16.158591048984036	17.117810638942853
3	6.2132293135012	9.87309622377592
4	3.145980278819687	6.665469102279184
5	1.7499756676833769	4.634647911342041
6	1.064345477801558	3.3825840919281918
7	0.704903956992173	2.613621661844874
8	0.47887670407700045	2.0292167095364593
9	0.34462121857263683	1.6428550822978887
>10	1.2808794408651976	10.781435945530019
>50	0.03410056983544941	1.2144792954012418
>100	0.013371428183544769	1.2772997181988477
>500	0.0011129590892434075	0.42834975413408616
>1k	5.136734258046139E-4	0.3853605982036077
>5k	8.561223763410232E-5	0.3091772838964695
>10k+	1.7122447526820463E-4	1.1976198397925724
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14234	0.38947844529310893	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12176	0.3331663306090273	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6818	0.18655782211665145	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.20876307348129E-5	0.0
3	0.0	0.0	0.0	8.20876307348129E-5	0.0
4	0.0	0.0	0.0	1.0945017431308387E-4	0.0
5	0.0	0.0	0.0	1.0945017431308387E-4	0.0
6	0.0	0.0	0.0	1.0945017431308387E-4	0.0
7	0.0	0.0	0.0	1.0945017431308387E-4	0.0
8	0.0	0.0	0.0	1.0945017431308387E-4	0.0
9	0.0	0.0	0.0	1.3681271789135483E-4	0.0
10-11	0.0	0.0	0.0	2.462628922044387E-4	0.0
12-13	0.0	0.0	0.0	2.462628922044387E-4	0.0
14-15	0.0	0.0	0.0	2.462628922044387E-4	0.0
16-17	0.0	0.0	0.0	2.7362543578270967E-4	0.0
18-19	0.0	0.0	0.0	2.7362543578270967E-4	0.0
20-21	0.0	0.0	0.0	3.5571306651752257E-4	0.0
22-23	0.0	0.0	0.0	6.019759587219612E-4	0.0
24-25	0.0	0.0	0.0	0.0011629081020765161	0.0
26-27	0.0	0.0	0.0	0.0020521907683703224	0.0
28-29	0.0	0.0	0.0	0.005198883279871483	0.0
30-31	0.0	0.0	0.0	0.013599184158400671	0.0
32-33	0.0	0.0	0.0	0.03200049471478789	0.0
34-35	0.0	0.0	0.0	0.06204456756372942	0.0
36-37	0.0	0.0	0.0	0.10862929800573573	0.0
38-39	0.0	0.0	0.0	0.1726166061635224	0.0
40-41	0.0	0.0	0.0	0.24318460605188322	0.0
42-43	0.0	0.0	0.0	0.3105238257980081	0.0
44-45	0.0	0.0	0.0	0.38912273222659144	0.0
46-47	0.0	0.0	0.0	0.4840297146278243	0.0
48-49	0.0	0.0	0.0	0.5840398114064047	0.0
50-51	0.0	0.0	0.0	0.6866903936402882	0.0
52-53	0.0	0.0	0.0	0.7821446269130864	0.0
54-55	0.0	0.0	0.0	0.8842205957318263	0.0
56-57	0.0	0.0	0.0	1.0041095804200204	1.3681271789135484E-5
58-59	0.0	0.0	0.0	1.137556705451248	2.7362543578270967E-5
60-61	0.0	0.0	0.0	1.2740684353632419	2.7362543578270967E-5
62-63	0.0	0.0	0.0	1.4133848259920083	2.7362543578270967E-5
64-65	0.0	0.0	0.0	1.55389148726643	2.7362543578270967E-5
66-67	0.0	0.0	0.0	1.7038245448035658	2.7362543578270967E-5
68-69	0.0	0.0	0.0	1.8709412797078557	2.7362543578270967E-5
70-71	0.0	0.0	0.0	2.049427151468917	2.7362543578270967E-5
72-73	0.0	0.0	0.0	2.229664226018988	2.7362543578270967E-5
74-75	0.0	0.0	0.0	2.4053454370632767	2.7362543578270967E-5
76-77	0.0	0.0	0.0	2.597663074603154	2.7362543578270967E-5
78-79	0.0	0.0	0.0	2.806767632628301	2.7362543578270967E-5
80-81	0.0	0.0	0.0	3.02975868151942	2.7362543578270967E-5
82-83	0.0	0.0	0.0	3.2597545415665765	2.7362543578270967E-5
84-85	0.0	0.0	0.0	3.4865353027432864	2.7362543578270967E-5
86-87	0.0	0.0	0.0	3.7259301965095792	2.7362543578270967E-5
88	0.0	0.0	0.0	3.9119681302982436	2.7362543578270967E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8525	0.0	51.123043	1
GTATCAA	15140	0.0	42.27787	1
ATCAACG	18965	0.0	33.495026	3
CAACGCA	19035	0.0	33.322445	5
TATCAAC	19330	0.0	33.0884	2
TCAACGC	19525	0.0	32.606594	4
AACGCAG	19890	0.0	32.066864	6
ACGCAGA	21845	0.0	28.978733	7
CGCAGAG	22220	0.0	28.426216	8
GCAGAGT	24090	0.0	25.809933	9
GTACATG	11850	0.0	24.704273	1
GAGTACT	16495	0.0	23.348581	12-13
TACATGG	12200	0.0	23.33908	2
GTACTAG	1510	0.0	23.065075	1
ACATGGG	12130	0.0	22.249943	3
CAGAGTA	23595	0.0	21.7902	10-11
GTACTTT	17620	0.0	21.564428	14-15
AGAGTAC	22485	0.0	21.486422	10-11
AGTACTT	17035	0.0	21.035923	12-13
CATGGGG	8445	0.0	20.711954	4
>>END_MODULE