FastQCFastQC Report
Mon 16 Jan 2017
ERR1146492_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146492_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583307
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29850.041.107951
GTATCAA45450.032.894471
ATCAACG49400.029.203793
CAACGCA49550.028.830875
GTACATG79350.028.5581281
TCAACGC50700.028.270154
TACATGG81500.027.27292
AACGCAG53450.026.9004486
ACATGGG78350.026.2101963
TATCAAC56200.026.0047042
ACGCAGA61100.023.1469767
GTACTAG7250.022.696541
CGCAGAG62450.022.1212188
CATGGGG57600.022.109714
CTAACGC3101.2732926E-1121.2223833
TAACGCC3456.184564E-1119.0697574
TCTAACG3255.638867E-1018.7969682
GCAGAGT70600.018.5692219
GTGATCG3559.276846E-1118.5307878
TGATCGC3701.6552804E-1017.7795419
Produced by FastQC (version 0.11.5)