##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146492_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2583307 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.259473225598043 31.0 31.0 34.0 28.0 34.0 2 31.299606666958283 31.0 31.0 34.0 28.0 34.0 3 31.48077986859479 33.0 31.0 34.0 28.0 34.0 4 35.05452700743659 37.0 35.0 37.0 32.0 37.0 5 35.00879337995833 37.0 35.0 37.0 32.0 37.0 6 35.176425798404914 37.0 35.0 37.0 32.0 37.0 7 35.04998631598954 37.0 35.0 37.0 32.0 37.0 8 35.04106983800222 37.0 35.0 37.0 32.0 37.0 9 36.67601605229266 39.0 37.0 39.0 32.0 39.0 10-11 36.67574179143246 39.0 37.0 39.0 32.0 39.0 12-13 36.64014458986098 39.0 37.0 39.0 32.0 39.0 14-15 38.03207032691043 40.0 38.0 41.0 32.5 41.0 16-17 38.02702950133298 40.0 38.0 41.0 32.5 41.0 18-19 38.03507868015687 40.0 38.0 41.0 32.0 41.0 20-21 38.148185639569746 40.0 38.0 41.0 33.0 41.0 22-23 38.15070798786207 40.0 38.0 41.0 32.5 41.0 24-25 38.20419543631477 40.0 38.0 41.0 33.0 41.0 26-27 38.25087823475878 40.0 38.0 41.0 33.0 41.0 28-29 38.21340185274147 40.0 38.0 41.0 33.0 41.0 30-31 38.1928928694886 40.0 38.0 41.0 33.0 41.0 32-33 38.21448883156357 40.0 38.0 41.0 33.0 41.0 34-35 38.159977114605425 40.0 38.0 41.0 33.0 41.0 36-37 38.06607867357616 40.0 38.0 41.0 32.0 41.0 38-39 38.03128664150254 40.0 38.0 41.0 32.0 41.0 40-41 37.97366534445964 40.0 38.0 41.0 32.0 41.0 42-43 37.844291057934655 40.0 38.0 41.0 32.0 41.0 44-45 37.69309145215803 40.0 37.5 41.0 31.5 41.0 46-47 37.588538450908075 40.0 37.0 41.0 31.0 41.0 48-49 37.48659508916285 40.0 37.0 41.0 31.0 41.0 50-51 37.37620170579804 40.0 37.0 41.0 31.0 41.0 52-53 37.27579958556997 40.0 36.5 41.0 31.0 41.0 54-55 37.107015542481015 40.0 36.0 41.0 30.0 41.0 56-57 36.99554950302074 39.0 35.5 41.0 30.0 41.0 58-59 36.79600392055609 39.0 35.0 41.0 30.0 41.0 60-61 36.626908648488154 39.0 35.0 41.0 30.0 41.0 62-63 36.40186648354222 38.5 35.0 40.5 30.0 41.0 64-65 36.159145622258606 38.0 35.0 40.0 30.0 41.0 66-67 35.894501698791515 37.0 35.0 40.0 29.5 41.0 68-69 35.599181398107156 37.0 35.0 40.0 29.0 41.0 70-71 35.310774522733844 36.0 34.5 39.0 29.0 41.0 72-73 34.98084528861649 36.0 34.0 39.0 29.0 41.0 74-75 34.65426215312388 35.5 34.0 38.5 29.0 40.0 76-77 34.333839919142406 35.0 34.0 37.0 29.0 39.5 78-79 34.0459757589787 35.0 34.0 37.0 28.0 39.0 80-81 33.75306128926992 35.0 34.0 36.5 28.0 39.0 82-83 33.50109336598399 35.0 34.0 36.0 28.0 37.5 84-85 33.28520458466609 35.0 34.0 36.0 28.0 37.0 86-87 33.092658170322 35.0 33.5 35.0 27.5 37.0 88-89 32.940596878342376 35.0 33.0 35.0 27.0 36.0 90-91 32.78840532697043 35.0 33.0 35.0 27.0 36.0 92-93 32.705137058816476 35.0 33.0 35.0 27.0 36.0 94-95 32.60787858353653 35.0 33.0 35.0 27.0 36.0 96-97 32.53955220962898 35.0 33.0 35.0 27.0 35.0 98-99 32.473332437840334 35.0 33.0 35.0 26.5 35.0 100 31.57056594512383 34.0 31.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11419210192736529 1101 2 0.2156643117049022 1101 3 0.24763362862620752 1101 4 0.2753142142802787 1101 5 0.16255963211852276 1101 6 0.26988275074130996 1101 7 0.2293166742569852 1101 8 0.2565132497783864 1101 9 0.39697085234934093 1101 10-11 0.43700202299061175 1101 12-13 0.40624916515845655 1101 14-15 0.47795820964923763 1101 16-17 0.4893060342872886 1101 18-19 0.3851186500762438 1101 20-21 0.38090618163725054 1101 22-23 0.38580701807021 1101 24-25 0.3562327279314488 1101 26-27 0.3544521676567953 1101 28-29 0.425004864165075 1101 30-31 0.4494899397925778 1101 32-33 0.429909563035217 1101 34-35 0.4123113981572999 1101 36-37 0.44887382643779716 1101 38-39 0.388688654123051 1101 40-41 0.38469554763246094 1101 42-43 0.3478887552334058 1101 44-45 0.23569734713968415 1101 46-47 0.3374050057980682 1101 48-49 0.37508509560876035 1101 50-51 0.34550309672675183 1101 52-53 0.24925385157051494 1101 54-55 0.2948131066687125 1101 56-57 0.19049251511678733 1101 58-59 0.23970470704189495 1101 60-61 0.2768592059173045 1101 62-63 0.2912036406078471 1101 64-65 0.30603579879298337 1101 66-67 0.22582046029894087 1101 68-69 0.22123705590757936 1101 70-71 0.16988409700601892 1101 72-73 0.1689221910388241 1101 74-75 0.1843727721116437 1101 76-77 0.105045360587674 1101 78-79 0.17509769175467937 1101 80-81 0.21973073373795415 1101 82-83 0.21786980198404393 1101 84-85 0.273851675886263 1101 86-87 0.16361609226185436 1101 88-89 0.09227583510541137 1101 90-91 0.18577284021043994 1101 92-93 0.14439537872129193 1101 94-95 0.12314795415371549 1101 96-97 0.14990492883040218 1101 98-99 0.20705385649821295 1101 100 0.1667010490994194 1102 1 0.329807589237344 1102 2 0.31050502332090346 1102 3 0.31957683970878037 1102 4 0.38273593510704984 1102 5 0.4210077539940116 1102 6 0.35748300292507906 1102 7 0.2684765120955106 1102 8 0.3607513056055822 1102 9 0.4868693170134506 1102 10-11 0.4687588314526252 1102 12-13 0.5495639426242036 1102 14-15 0.5921269063217238 1102 16-17 0.6949941554182217 1102 18-19 0.553112260340626 1102 20-21 0.5843914433985518 1102 22-23 0.547035426385932 1102 24-25 0.6372662229413777 1102 26-27 0.6213311763222578 1102 28-29 0.5995821909274781 1102 30-31 0.5348784134499525 1102 32-33 0.5746953119634526 1102 34-35 0.5703991860995643 1102 36-37 0.568026091179739 1102 38-39 0.6050818315799731 1102 40-41 0.5609940562115696 1102 42-43 0.5833952978442198 1102 44-45 0.5114929703931281 1102 46-47 0.4930774257660886 1102 48-49 0.4990450443722949 1102 50-51 0.4753951895797357 1102 52-53 0.4473336303685329 1102 54-55 0.43162663752072206 1102 56-57 0.4222179384880391 1102 58-59 0.47767780583099295 1102 60-61 0.4306876009964853 1102 62-63 0.47159349996610445 1102 64-65 0.4134122344325206 1102 66-67 0.4189179205712037 1102 68-69 0.45262729995294393 1102 70-71 0.464536885866913 1102 72-73 0.4254883708373072 1102 74-75 0.3230286474316557 1102 76-77 0.4179436849581819 1102 78-79 0.4658729741601775 1102 80-81 0.3791717775085246 1102 82-83 0.38898020511614106 1102 84-85 0.4136541152713349 1102 86-87 0.3595891834675129 1102 88-89 0.2821235338250858 1102 90-91 0.36558192027396075 1102 92-93 0.4741358717619377 1102 94-95 0.3832494808720597 1102 96-97 0.3266176826902907 1102 98-99 0.3006160263816895 1102 100 0.17526576632853974 1103 1 0.051359498449020435 1103 2 0.04776549613645287 1103 3 0.13316381297796198 1103 4 0.13569884251799635 1103 5 0.03110781516492267 1103 6 0.024115420919372355 1103 7 0.05501761807849448 1103 8 0.13089493040675393 1103 9 0.30541440276594045 1103 10-11 0.24323396274535014 1103 12-13 0.22786978082825726 1103 14-15 0.29208738272955515 1103 16-17 0.1841414264686847 1103 18-19 0.11439834423960349 1103 20-21 0.16857958897681158 1103 22-23 0.1846672064525876 1103 24-25 0.18637281780557657 1103 26-27 0.21502690373937128 1103 28-29 0.21801893794260252 1103 30-31 0.19799550458167658 1103 32-33 0.198547633844683 1103 34-35 0.16003010401903595 1103 36-37 0.10409628501133028 1103 38-39 0.11621287710199368 1103 40-41 0.0926797531658039 1103 42-43 0.07983047072567473 1103 44-45 0.13850441308169792 1103 46-47 0.10411224585499923 1103 48-49 0.10328228330845235 1103 50-51 0.03222335618729488 1103 52-53 0.013163693052966607 1103 54-55 0.09003584145276022 1103 56-57 0.06591228687825179 1103 58-59 0.13448197566786746 1103 60-61 0.06672234872905136 1103 62-63 0.061421823033633416 1103 64-65 0.08734417383637805 1103 66-67 0.10215871722653702 1103 68-69 0.09030219589173782 1103 70-71 0.09596053365867618 1103 72-73 0.14606278832186348 1103 74-75 0.09838733488405893 1103 76-77 0.07324943990986554 1103 78-79 0.042052037909861895 1103 80-81 0.041927469952831586 1103 82-83 0.07264317979294788 1103 84-85 0.06521172616339754 1103 86-87 0.08236451087147856 1103 88-89 -0.017299683914586694 1103 90-91 0.049597823688962706 1103 92-93 0.12492842065823595 1103 94-95 -0.03472689248125249 1103 96-97 -0.013073674062788143 1103 98-99 -0.0475285950643638 1103 100 -0.09909819156040456 1104 1 0.10671116663193914 1104 2 0.05666564131823293 1104 3 0.057393953078456406 1104 4 0.025656354599924214 1104 5 0.07892943941483566 1104 6 0.11936713372187313 1104 7 0.13059597190196115 1104 8 0.14267290597248206 1104 9 0.16282409267623876 1104 10-11 0.24514003077450042 1104 12-13 0.13893272478570395 1104 14-15 0.19896245085926978 1104 16-17 0.21889790252541985 1104 18-19 0.13671642636998627 1104 20-21 0.19481865831202327 1104 22-23 0.12836625774291122 1104 24-25 0.1619324120097545 1104 26-27 0.18076291656253574 1104 28-29 0.1405354935607761 1104 30-31 0.18704947394977012 1104 32-33 0.1482284935435274 1104 34-35 0.13932997062946129 1104 36-37 0.19152279680283613 1104 38-39 0.07228299413105077 1104 40-41 0.1237953069467963 1104 42-43 0.11288114296752383 1104 44-45 0.046260260585626156 1104 46-47 0.06402828033266417 1104 48-49 0.044467788026672395 1104 50-51 0.07009260750567137 1104 52-53 -0.023073416648806244 1104 54-55 0.07894025779087599 1104 56-57 0.050005005534167424 1104 58-59 0.10178178535039706 1104 60-61 0.0702090652138807 1104 62-63 -0.04213764855660429 1104 64-65 0.0570034415965921 1104 66-67 -0.035394451319298526 1104 68-69 -0.04211686600843478 1104 70-71 -0.05802176833351069 1104 72-73 0.06540135182768125 1104 74-75 -0.04895469528053553 1104 76-77 -0.012453478098471749 1104 78-79 0.041556968507094894 1104 80-81 9.539669180682608E-4 1104 82-83 0.016647319264428972 1104 84-85 -0.0015155906104880046 1104 86-87 -0.030176079580300552 1104 88-89 0.037308285396903784 1104 90-91 0.023850461257026723 1104 92-93 0.10274620696685588 1104 94-95 0.04428156271922745 1104 96-97 -0.03284743884077557 1104 98-99 -1.0296377853791228E-4 1104 100 -0.03149263683635084 1105 1 -0.024558135402919845 1105 2 0.0598396362773137 1105 3 0.08976184811054466 1105 4 0.18244044617404143 1105 5 0.052863473100920544 1105 6 0.13760091589601586 1105 7 0.11236419238868933 1105 8 0.14759967422746456 1105 9 0.2652464000041306 1105 10-11 0.24901974117193504 1105 12-13 0.24229416880210408 1105 14-15 0.2750432034859216 1105 16-17 0.2572220447579596 1105 18-19 0.2223638899666156 1105 20-21 0.24160025991112377 1105 22-23 0.25077979341619994 1105 24-25 0.21931498765859914 1105 26-27 0.14952696995703008 1105 28-29 0.2507252323270137 1105 30-31 0.21353900303741824 1105 32-33 0.25460472097994113 1105 34-35 0.2150898524813698 1105 36-37 0.27582269215267985 1105 38-39 0.22806186120799765 1105 40-41 0.22421495340594788 1105 42-43 0.20901031103326773 1105 44-45 0.13676462182470317 1105 46-47 0.024186566197371917 1105 48-49 0.05979181521746568 1105 50-51 0.046064126107829395 1105 52-53 0.10219402202137218 1105 54-55 0.11925504492694472 1105 56-57 0.022724870439112976 1105 58-59 0.0817260268214497 1105 60-61 0.10313659269984754 1105 62-63 0.1756973370832 1105 64-65 0.14009005823678677 1105 66-67 0.07443903633018323 1105 68-69 0.07440740034456894 1105 70-71 0.0994707088934561 1105 72-73 0.1512624325836427 1105 74-75 0.21204875797435818 1105 76-77 0.21385454315910124 1105 78-79 0.12552008305417672 1105 80-81 0.17240921823976407 1105 82-83 0.126965228326263 1105 84-85 0.19652231352925753 1105 86-87 0.13006527220688469 1105 88-89 0.1018653936080085 1105 90-91 0.13762729941598906 1105 92-93 0.1121379076286928 1105 94-95 0.10658411982787896 1105 96-97 0.08776648352407079 1105 98-99 0.043571175705899634 1105 100 -0.01996888116186568 1106 1 -0.02161740956809055 1106 2 -0.0028985948065027856 1106 3 0.02548366101401811 1106 4 0.08184045347852731 1106 5 0.07362181291476588 1106 6 0.031980644329280494 1106 7 0.001039959382545419 1106 8 -0.01599754721532065 1106 9 0.06967933542085092 1106 10-11 0.007510321495949768 1106 12-13 0.12166249955659225 1106 14-15 0.1912338533807656 1106 16-17 0.09292009579497318 1106 18-19 0.09481800751726155 1106 20-21 0.00984912838306684 1106 22-23 0.06592048949858054 1106 24-25 0.04372615212812292 1106 26-27 0.0960554213959739 1106 28-29 0.10767846220380761 1106 30-31 0.024497989209656623 1106 32-33 0.08854947141130509 1106 34-35 0.1285501147126098 1106 36-37 0.12394294367660308 1106 38-39 0.09990979506137165 1106 40-41 0.05850844875645578 1106 42-43 0.05718801861667089 1106 44-45 -0.013928703562768874 1106 46-47 0.029391684329702628 1106 48-49 -0.05500349868413679 1106 50-51 0.007765876602441324 1106 52-53 0.03648177176592782 1106 54-55 0.08894942323010468 1106 56-57 -0.05444284869785321 1106 58-59 -0.024368145887009973 1106 60-61 -0.10100467527076518 1106 62-63 -0.04102369014206886 1106 64-65 -0.010492474942971342 1106 66-67 -0.05557923825989519 1106 68-69 -0.012255934761441267 1106 70-71 0.026480245708540906 1106 72-73 -0.008032042637658776 1106 74-75 -0.052759093819290115 1106 76-77 -0.05532152804008916 1106 78-79 -0.03507528197886245 1106 80-81 -0.03307436135290942 1106 82-83 -0.10279520662947306 1106 84-85 -0.029584094202100175 1106 86-87 -0.11586236255429583 1106 88-89 0.00926637989249457 1106 90-91 -0.01511050408785053 1106 92-93 0.01416040546184405 1106 94-95 -0.036340945890266596 1106 96-97 -0.06666527159374169 1106 98-99 -0.07628784315838999 1106 100 0.004291383207270627 1107 1 0.10781854610821995 1107 2 0.11443462972176377 1107 3 0.180511286506988 1107 4 0.20705570287467623 1107 5 0.18283665753352096 1107 6 0.19082222556564687 1107 7 0.1432837650341554 1107 8 0.1604069612606267 1107 9 0.2581266054144109 1107 10-11 0.32955703213001897 1107 12-13 0.31408362081814545 1107 14-15 0.35441808854177026 1107 16-17 0.30361021446295666 1107 18-19 0.26468290178890186 1107 20-21 0.24177223110886104 1107 22-23 0.1983230039666921 1107 24-25 0.2979242376663862 1107 26-27 0.31420647383127687 1107 28-29 0.29598616715266246 1107 30-31 0.3215712950711236 1107 32-33 0.3170829863307034 1107 34-35 0.20955189082843617 1107 36-37 0.30026655305064054 1107 38-39 0.2614598401760091 1107 40-41 0.2744098648853708 1107 42-43 0.2914520157820988 1107 44-45 0.28517314023205387 1107 46-47 0.2713856329641331 1107 48-49 0.2790628242036277 1107 50-51 0.29746584241059537 1107 52-53 0.2566187632608177 1107 54-55 0.29418959913350307 1107 56-57 0.320185882209465 1107 58-59 0.3072183869776097 1107 60-61 0.22575507751060542 1107 62-63 0.2649658524510272 1107 64-65 0.19652818589839427 1107 66-67 0.19738510073947424 1107 68-69 0.2656505205076982 1107 70-71 0.19263738192542945 1107 72-73 0.16480550604627098 1107 74-75 0.17139878740221803 1107 76-77 0.17060972068968994 1107 78-79 0.1308067409158653 1107 80-81 0.2078206843240693 1107 82-83 0.17913967861360192 1107 84-85 0.2447216111013546 1107 86-87 0.1890181999284195 1107 88-89 0.12724894655885066 1107 90-91 0.18234729096344182 1107 92-93 0.18999763365001598 1107 94-95 0.16282510275799922 1107 96-97 0.10734041643056713 1107 98-99 0.053659757055719126 1107 100 0.04657469411717585 1108 1 0.2001618089122985 1108 2 0.19441959446067258 1108 3 0.20939498530315248 1108 4 0.14217438952129413 1108 5 0.11096237055367908 1108 6 0.13025155496769258 1108 7 0.17652860406878546 1108 8 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56-57 -0.21328647658656763 2214 58-59 -0.19423305953434067 2214 60-61 -0.20795516260760394 2214 62-63 -0.16273888994278707 2214 64-65 -0.18938531150305238 2214 66-67 -0.253120862969368 2214 68-69 -0.2277882265006781 2214 70-71 -0.22810364441532016 2214 72-73 -0.18114270198318394 2214 74-75 -0.17091901461254366 2214 76-77 -0.12115105619838573 2214 78-79 -0.0926883236489644 2214 80-81 -0.13974962000981606 2214 82-83 -0.14104038234305705 2214 84-85 -0.08803627988197604 2214 86-87 -0.05637259377805748 2214 88-89 -0.1641707011952036 2214 90-91 -0.09045508089538146 2214 92-93 -0.09448341115511028 2214 94-95 -0.10451479964066124 2214 96-97 -0.0947367497749454 2214 98-99 -0.11630524879955573 2214 100 -0.049843432930472886 2215 1 -0.2862638087267051 2215 2 -0.27268976059342265 2215 3 -0.3579124342400277 2215 4 -0.34937288662624866 2215 5 -0.30484810188389844 2215 6 -0.34095145393589377 2215 7 -0.3175409458658649 2215 8 -0.2847482338145255 2215 9 -0.42561487121264463 2215 10-11 -0.37689878897763407 2215 12-13 -0.40252019976428244 2215 14-15 -0.4963694741716296 2215 16-17 -0.5287099402413489 2215 18-19 -0.5986450502939391 2215 20-21 -0.5273749037596858 2215 22-23 -0.49104315721213254 2215 24-25 -0.5295794561042229 2215 26-27 -0.48933872256564115 2215 28-29 -0.43640413320422056 2215 30-31 -0.4520769342964357 2215 32-33 -0.5313563001641199 2215 34-35 -0.5203170866460027 2215 36-37 -0.47162626282182174 2215 38-39 -0.4942050592535381 2215 40-41 -0.5073644416956284 2215 42-43 -0.557060175514863 2215 44-45 -0.31970982384054736 2215 46-47 -0.3462367227781513 2215 48-49 -0.39716874893041876 2215 50-51 -0.33542859710688333 2215 52-53 -0.3627659856434846 2215 54-55 -0.3534970175296621 2215 56-57 -0.3840971358683092 2215 58-59 -0.39089179731612944 2215 60-61 -0.3850111459617622 2215 62-63 -0.38654033240631946 2215 64-65 -0.4298063754927881 2215 66-67 -0.4372342980232986 2215 68-69 -0.35155649052409643 2215 70-71 -0.38498886498116036 2215 72-73 -0.37936076292479726 2215 74-75 -0.3817417518991988 2215 76-77 -0.4386108064671035 2215 78-79 -0.42458604965530355 2215 80-81 -0.33221370362239355 2215 82-83 -0.3063069251895243 2215 84-85 -0.31896618643420993 2215 86-87 -0.323724981482286 2215 88-89 -0.33840708433140776 2215 90-91 -0.3657845193920295 2215 92-93 -0.3503895546478688 2215 94-95 -0.43115867518738327 2215 96-97 -0.4044638634575719 2215 98-99 -0.3770500358095745 2215 100 -0.2359865971735502 2216 1 -0.04239128235536427 2216 2 -0.0011103002245285154 2216 3 -0.07200576048213847 2216 4 -0.10403172077719347 2216 5 -0.037555973620378325 2216 6 -0.09889425665274132 2216 7 -0.16478182019447019 2216 8 -0.10186056603968296 2216 9 -0.24141625126317479 2216 10-11 -0.3168845243942471 2216 12-13 -0.3066843081833355 2216 14-15 -0.40101259092833885 2216 16-17 -0.3917211705206043 2216 18-19 -0.33555045878875944 2216 20-21 -0.36570417812087186 2216 22-23 -0.32519555758421603 2216 24-25 -0.415327176269237 2216 26-27 -0.4447787976258226 2216 28-29 -0.4474357418508532 2216 30-31 -0.4875687698074458 2216 32-33 -0.4139409054342167 2216 34-35 -0.42155696402808474 2216 36-37 -0.3294917363012644 2216 38-39 -0.35591077042583663 2216 40-41 -0.41975785089682915 2216 42-43 -0.27776443284959385 2216 44-45 -0.24447689496701486 2216 46-47 -0.16203942901055512 2216 48-49 -0.16431089030174917 2216 50-51 -0.2131536869621229 2216 52-53 -0.15901405455155526 2216 54-55 -0.17307703198589763 2216 56-57 -0.2012581518023424 2216 58-59 -0.16489008508698788 2216 60-61 -0.14191601885021754 2216 62-63 -0.14018746090598455 2216 64-65 -0.160007635143117 2216 66-67 -0.17279115067028528 2216 68-69 -0.17983018331945289 2216 70-71 -0.13811998039080464 2216 72-73 -0.20423592215217212 2216 74-75 -0.19583659255545172 2216 76-77 -0.1739152139769473 2216 78-79 -0.19608598189169157 2216 80-81 -0.15261941602675932 2216 82-83 -0.16020125399091256 2216 84-85 -0.16308869381120417 2216 86-87 -0.18343093872550043 2216 88-89 -0.19803796256840656 2216 90-91 -0.16100727858957242 2216 92-93 -0.16893827018510876 2216 94-95 -0.19272666023285012 2216 96-97 -0.1726080421865035 2216 98-99 -0.1467851622092553 2216 100 -0.17075051284275844 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 63.0 1 48.0 2 39.0 3 27.0 4 30.0 5 40.0 6 68.0 7 270.0 8 931.0 9 1588.0 10 2171.0 11 3128.0 12 4398.0 13 5284.0 14 5970.0 15 6472.0 16 6978.0 17 7685.0 18 8204.0 19 9176.0 20 10261.0 21 11405.0 22 12672.0 23 14489.0 24 16821.0 25 20148.0 26 22798.0 27 22034.0 28 23672.0 29 30553.0 30 34084.0 31 37943.0 32 48414.0 33 65791.0 34 95518.0 35 146666.0 36 276654.0 37 607442.0 38 795169.0 39 227840.0 40 363.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.62095110827063 19.043159128102335 12.997317684881232 24.3385720787458 2 15.105530120547229 20.755353933754385 40.185958171993306 23.95315777370508 3 18.446387319546 25.74023451035018 30.120917584559653 25.69246058554417 4 12.165741086654808 17.315166285014122 38.80316600627549 31.71592662205558 5 13.102890249497193 38.461514637078274 35.15368787008879 13.28190724333574 6 30.52975204627829 37.98605642625461 18.33938447886977 13.144807048597318 7 26.843192027364125 32.85785735410934 22.51013844650663 17.7888121720199 8 25.558434459512558 35.92368878570773 20.484608187107337 18.033268567672373 9 26.086305134035896 16.109703249643648 20.036281790118437 37.767709826202015 10-11 24.34960064836209 26.527079898781498 28.952282400635355 20.17103705222106 12-13 24.913032403123943 24.39399476452357 28.416996174432263 22.275976657920225 14-15 22.40237875535006 25.893993162685007 25.658997964213754 26.044630117751176 16-17 22.391189762607283 28.741167627840134 26.30311061711963 22.564531992432947 18-19 22.373502670049685 28.359647988578057 27.415161948946576 21.851687392425685 20-21 23.241241265654754 27.527992779278872 27.185906663701264 22.044859291365118 22-23 23.149890689281744 27.45660717724789 26.77691613124254 22.61658600222783 24-25 22.796355525205577 27.565869902345852 27.05214621565981 22.585628356788757 26-27 22.647042712450734 27.76430272965053 26.820500938865994 22.768153619032745 28-29 22.602527735113338 27.8728331867096 26.8990967397269 22.625542338450163 30-31 22.845237834842234 27.662792649116057 26.874910353539512 22.617059162502198 32-33 22.65795932336068 27.87015097189135 26.716041486927857 22.755848217820116 34-35 22.911609550777122 27.820544685740657 26.853159550506117 22.414686212976104 36-37 22.914606989972427 27.609208276894886 26.822241585145896 22.65394314798679 38-39 22.826633627907427 27.709600921471644 26.879734399349548 22.58403105127138 40-41 22.835174144320046 27.496768078722983 27.012376738839468 22.655681038117507 42-43 22.715423655147916 27.474544294717973 26.95409503140642 22.855937018727694 44-45 22.738189389883964 27.6025064058941 26.96057144350664 22.69873276071529 46-47 22.82359086573116 27.561605124304144 26.786179294498215 22.828624715466482 48-49 22.767978842043306 27.565169914191014 26.84992720175955 22.816924042006136 50-51 22.721624494426838 27.66893258959947 26.85957021682772 22.74987269914597 52-53 22.81314684009508 27.65747153008134 26.768335336232457 22.761046293591132 54-55 22.6628845818731 27.57786512285321 26.89960694716053 22.859643348113153 56-57 22.792068744313372 27.619420861031085 26.80695567785562 22.781554716799928 58-59 22.79716150018394 27.538656649950628 26.924468023312098 22.739713826553334 60-61 22.67402798016829 27.642972680862893 26.929566294612346 22.75343304435647 62-63 22.712967224284114 27.601105231455996 26.938787285152777 22.747140259107113 64-65 22.586661100756782 27.542405534104685 26.87083694272005 23.000096422418483 66-67 22.65197092864755 27.69260080795063 26.714629699620808 22.940798563781012 68-69 22.649962968520825 27.52386715138372 26.933958108441725 22.892211771653734 70-71 22.659527461722586 27.405570376727116 26.92196430500796 23.012937856542333 72-73 22.685823952118533 27.56230015989145 26.87208335576099 22.87979253222903 74-75 22.73425469489136 27.780354075618202 26.755802914570587 22.729588314919848 76-77 22.811382601504583 27.500634606347536 26.729812983300416 22.95816980884747 78-79 22.73670489659757 27.59064691409467 26.77810033522266 22.894547854085108 80-81 22.769284926890958 27.687272638553605 26.738916301942776 22.804526132612658 82-83 22.895116144342417 27.57793040633481 26.880897833872385 22.64605561545039 84-85 22.855075570074323 27.746195454662505 26.6582171770534 22.74051179820977 86-87 22.740266984506285 27.791605169463157 26.788617484780346 22.67951036125021 88-89 22.875616675569553 27.485458353960983 26.737286962441186 22.90163800802828 90-91 22.869397580470753 27.56281887180925 26.71555961802858 22.852223929691423 92-93 22.946468971977417 27.57667820180417 26.826252096160953 22.65060073005745 94-95 22.784298121540758 27.50682772448232 26.92626057559097 22.782613578385956 96-97 22.85936059674178 27.36706430993987 26.93808742059542 22.83548767272293 98-99 22.744097561580055 27.564441632345808 26.744613342461466 22.946847463612666 100 22.880299300151247 27.39864893644209 26.66046609601798 23.06058566738869 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1034.0 1 830.5 2 687.5 3 736.0 4 698.5 5 637.5 6 551.5 7 497.0 8 543.5 9 571.5 10 509.0 11 455.5 12 446.5 13 476.0 14 521.5 15 576.5 16 682.0 17 780.0 18 961.5 19 1227.5 20 1577.0 21 2262.5 22 3419.5 23 4895.0 24 6750.0 25 9181.5 26 12544.0 27 16501.0 28 20365.0 29 24686.5 30 29604.5 31 35101.5 32 42036.0 33 47541.5 34 51711.0 35 56439.5 36 61216.0 37 67224.5 38 71941.0 39 74418.0 40 76746.0 41 79175.0 42 81574.0 43 85506.0 44 90513.5 45 96976.5 46 101767.5 47 103513.5 48 105388.0 49 104959.5 50 101406.0 51 98749.0 52 96405.5 53 92817.0 54 89143.5 55 82569.0 56 75337.0 57 68005.0 58 60396.0 59 53283.5 60 44321.0 61 35854.5 62 28790.5 63 21769.5 64 15327.0 65 10752.5 66 8071.5 67 6012.0 68 4216.0 69 2997.5 70 2257.0 71 1658.5 72 1234.0 73 943.0 74 697.5 75 480.5 76 329.0 77 238.5 78 177.0 79 136.5 80 102.5 81 80.0 82 60.0 83 50.0 84 39.0 85 26.5 86 19.5 87 19.0 88 20.5 89 15.5 90 10.0 91 7.5 92 6.0 93 8.0 94 7.5 95 2.0 96 0.5 97 2.0 98 4.5 99 3.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.061239333923532896 2 0.009793648219123782 3 0.011922702179802866 4 0.009367837426987964 5 0.012077542467852252 6 0.012580773404012764 7 0.0027484151128766344 8 0.005729090657827351 9 0.0074710438983829645 10-11 0.007064588142253321 12-13 0.006232321593987861 14-15 0.010664624839401587 16-17 0.010316234191290467 18-19 0.008090405050580516 20-21 0.007180718358290363 22-23 0.002883900364919849 24-25 0.004219397849345819 26-27 0.0053226349016977075 28-29 0.006367806846031075 30-31 0.009425902535006485 32-33 0.010703334911413936 34-35 0.012116252539864598 36-37 0.018522769457908022 38-39 0.018658254709951236 40-41 0.01850341442190185 42-43 0.02456154069183415 44-45 0.028045447172945374 46-47 0.030600311925760276 48-49 0.03201322955421094 50-51 0.032593880634396145 52-53 0.030987412645883745 54-55 0.037045538915816044 56-57 0.04035525007287171 58-59 0.037045538915816044 60-61 0.037858450428075334 62-63 0.03334872703863691 64-65 0.0356132662513592 66-67 0.0375874799239889 68-69 0.0404326702168964 70-71 0.040258474892840845 72-73 0.03600036697148268 74-75 0.03892297740841487 76-77 0.038613296832316096 78-79 0.03845845654426671 80-81 0.04267785439361253 82-83 0.038826202228384 84-85 0.034935839991143136 86-87 0.03377453783077272 88-89 0.03137451336600722 90-91 0.03338743711064925 92-93 0.03512939035120487 94-95 0.038884267336402524 96-97 0.03420034862290854 98-99 0.03154870869006277 100 0.02899384393724788 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2583307.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.61233904663798 #Duplication Level Percentage of deduplicated Percentage of total 1 75.28131411093783 49.39383105323305 2 14.47135762134499 18.989992454336722 3 4.9988560274435585 9.8395990955387 4 2.205564944120187 5.788490996119716 5 1.1096550482358964 3.6403531624833536 6 0.6160549111981265 2.425248222292676 7 0.36799441845237785 1.6901482185537453 8 0.2327783058407599 1.2218503300420747 9 0.156051763645137 0.9215029102599491 >10 0.5478586788736717 5.471007740843863 >50 0.010543715167024733 0.44821193262128783 >100 0.001970454740531706 0.16976388367485698 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.8710072012346966E-5 2 0.0 0.0 0.0 0.0 3.8710072012346966E-5 3 0.0 0.0 0.0 3.8710072012346966E-5 7.742014402469393E-5 4 0.0 0.0 0.0 3.8710072012346966E-5 7.742014402469393E-5 5 0.0 0.0 0.0 3.8710072012346966E-5 7.742014402469393E-5 6 0.0 0.0 0.0 3.8710072012346966E-5 1.1613021603704089E-4 7 0.0 0.0 0.0 3.8710072012346966E-5 1.1613021603704089E-4 8 0.0 0.0 0.0 1.1613021603704089E-4 1.1613021603704089E-4 9 0.0 0.0 0.0 1.935503600617348E-4 1.1613021603704089E-4 10-11 0.0 0.0 0.0 2.1290539606790828E-4 1.5484028804938786E-4 12-13 0.0 0.0 0.0 2.516154680802553E-4 6.387161882037249E-4 14-15 0.0 0.0 0.0 2.7097050408642873E-4 7.742014402469394E-4 16-17 0.0 0.0 0.0 3.0968057609877573E-4 9.677518003086741E-4 18-19 0.0 0.0 0.0 3.0968057609877573E-4 0.001122592088358062 20-21 0.0 0.0 0.0 3.483906481111227E-4 0.0014903377724753582 22-23 0.0 0.0 0.0 4.8387590015433707E-4 0.0016258230245185725 24-25 0.0 0.0 0.0 8.903316562839801E-4 0.0017032431685432665 26-27 0.0 0.0 0.0 0.0014516277004630112 0.0018580834565926543 28-29 0.0 0.0 0.0 0.003774232021203829 0.0018967935286050013 30-31 0.0 0.0 0.0 0.009754938147111435 0.0019355036006173483 32-33 3.8710072012346966E-5 0.0 0.0 0.023980889611648945 0.0021096989246729096 34-35 3.8710072012346966E-5 0.0 0.0 0.047574678503174414 0.0021290539606790833 36-37 3.8710072012346966E-5 0.0 0.0 0.08353633540264474 0.0021290539606790833 38-39 3.8710072012346966E-5 0.0 0.0 0.13635622866349217 0.0023419593567469916 40-41 3.8710072012346966E-5 0.0 0.0 0.19134388595703106 0.002380669428759338 42-43 3.8710072012346966E-5 0.0 0.0 0.24503475583815632 0.0024193795007716856 44-45 3.8710072012346966E-5 0.0 0.0 0.31260318653570796 0.0024967996447963796 46-47 3.8710072012346966E-5 0.0 0.0 0.3894039694082043 0.002593574824827247 48-49 3.8710072012346966E-5 0.0 0.0 0.47133383682233665 0.002845190292907502 50-51 3.8710072012346966E-5 0.0 0.0 0.558837954606247 0.0029806755449507164 52-53 3.8710072012346966E-5 0.0 0.0 0.6406516918043423 0.003135515833000104 54-55 3.8710072012346966E-5 0.0 0.0 0.727420318220018 0.003135515833000104 56-57 3.8710072012346966E-5 0.0 0.0 0.8280665054521201 0.0031935809410186247 58-59 3.8710072012346966E-5 0.0 0.0 0.9382160153632534 0.003251646049037145 60-61 3.8710072012346966E-5 0.0 0.0 1.0571140015491771 0.003290356121049492 62-63 3.8710072012346966E-5 0.0 0.0 1.1733216377302427 0.00344519640909888 64-65 3.8710072012346966E-5 0.0 0.0 1.2859485922501661 0.00344519640909888 66-67 3.8710072012346966E-5 0.0 0.0 1.4149499072313123 0.003483906481111227 68-69 3.8710072012346966E-5 0.0 0.0 1.559628801377459 0.003522616553123574 70-71 3.8710072012346966E-5 0.0 0.0 1.7125722958982421 0.0036193917331544413 72-73 3.8710072012346966E-5 0.0 0.0 1.8658061159591175 0.0038710072012346965 74-75 3.8710072012346966E-5 0.0 0.0 2.0184786399758137 0.004122622669314952 76-77 3.8710072012346966E-5 0.0 0.0 2.1841771032246653 0.004316173029376686 78-79 3.8710072012346966E-5 0.0 0.0 2.366501542402819 0.0044516582814199 80-81 3.8710072012346966E-5 0.0 0.0 2.565064856790153 0.004548433461450768 82-83 3.8710072012346966E-5 0.0 0.0 2.770325013635623 0.004954889217580412 84-85 3.8710072012346966E-5 0.0 0.0 2.967901221186642 0.005032309361605106 86-87 3.8710072012346966E-5 0.0 0.0 3.1700258621991115 0.0053226349016977075 88 3.8710072012346966E-5 0.0 0.0 3.3294532937819623 0.00561296044179031 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2985 0.0 41.10795 1 GTATCAA 4545 0.0 32.89447 1 ATCAACG 4940 0.0 29.20379 3 CAACGCA 4955 0.0 28.83087 5 GTACATG 7935 0.0 28.558128 1 TCAACGC 5070 0.0 28.27015 4 TACATGG 8150 0.0 27.2729 2 AACGCAG 5345 0.0 26.900448 6 ACATGGG 7835 0.0 26.210196 3 TATCAAC 5620 0.0 26.004704 2 ACGCAGA 6110 0.0 23.146976 7 GTACTAG 725 0.0 22.69654 1 CGCAGAG 6245 0.0 22.121218 8 CATGGGG 5760 0.0 22.10971 4 CTAACGC 310 1.2732926E-11 21.222383 3 TAACGCC 345 6.184564E-11 19.069757 4 TCTAACG 325 5.638867E-10 18.796968 2 GCAGAGT 7060 0.0 18.569221 9 GTGATCG 355 9.276846E-11 18.530787 8 TGATCGC 370 1.6552804E-10 17.779541 9 >>END_MODULE