FastQCFastQC Report
Mon 16 Jan 2017
ERR1146492_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146492_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583307
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100390.3886104129319512No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88920.3442099603337892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49570.1918858269652039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48950.059.12321
GTATCAA96000.045.7347031
ATCAACG120000.036.2046553
TCAACGC120350.036.0993654
CAACGCA121250.035.7926375
TATCAAC123950.035.3444982
AACGCAG126950.034.209356
ACGCAGA142300.030.3510787
CGCAGAG144900.029.7091778
GCAGAGT156600.027.0993889
GTACATG78150.024.4473921
ACATGGG74350.023.5253283
TACATGG79700.023.2034662
GAGTACT113400.023.16659712-13
TGGTATC17100.022.840242
CAGAGTA152200.022.57180210-11
AGAGTAC145250.022.47085810-11
GTGGTAT18200.021.977741
CATGGGG53650.021.384244
GTACTTT122650.021.1703614-15
Produced by FastQC (version 0.11.5)