##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146492_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2583307
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51628319824163	34.0	31.0	34.0	31.0	34.0
2	32.58500944719307	34.0	31.0	34.0	31.0	34.0
3	32.67806575060572	34.0	31.0	34.0	31.0	34.0
4	36.17564308074882	37.0	37.0	37.0	35.0	37.0
5	36.19117240033802	37.0	37.0	37.0	35.0	37.0
6	36.26715098128097	37.0	37.0	37.0	35.0	37.0
7	36.2545806595964	37.0	37.0	37.0	35.0	37.0
8	36.264980507543235	37.0	37.0	37.0	35.0	37.0
9	38.07283029078619	39.0	38.0	39.0	37.0	39.0
10-11	38.03430273676338	39.0	38.0	39.0	35.0	39.0
12-13	38.0120889619391	39.0	38.0	39.0	35.0	39.0
14-15	39.47361115035882	41.0	39.0	41.0	37.0	41.0
16-17	39.47001324271564	41.0	39.0	41.0	36.5	41.0
18-19	39.52062646832142	41.0	39.5	41.0	37.0	41.0
20-21	39.55514346533339	41.0	40.0	41.0	37.0	41.0
22-23	39.515595900913056	41.0	39.0	41.0	37.0	41.0
24-25	39.508290536122885	41.0	39.0	41.0	37.0	41.0
26-27	39.440964236925765	41.0	39.0	41.0	36.0	41.0
28-29	39.35189139347356	41.0	39.0	41.0	36.0	41.0
30-31	39.251625145598254	41.0	39.0	41.0	36.0	41.0
32-33	39.13133301616881	41.0	39.0	41.0	35.0	41.0
34-35	39.05195433605065	41.0	39.0	41.0	35.0	41.0
36-37	38.96872593927087	41.0	39.0	41.0	35.0	41.0
38-39	38.878763925464526	40.5	39.0	41.0	35.0	41.0
40-41	38.7921394940671	40.0	38.0	41.0	35.0	41.0
42-43	38.70202941423532	40.0	38.0	41.0	35.0	41.0
44-45	38.56871715208452	40.0	38.0	41.0	35.0	41.0
46-47	38.3391664250513	40.0	38.0	41.0	34.0	41.0
48-49	38.14284790773996	40.0	38.0	41.0	34.0	41.0
50-51	38.01332458743773	40.0	38.0	41.0	33.5	41.0
52-53	37.902077840535405	40.0	37.5	41.0	33.0	41.0
54-55	37.77532635493962	40.0	37.0	41.0	33.0	41.0
56-57	37.603638088697934	40.0	37.0	41.0	33.0	41.0
58-59	37.41496287510543	40.0	36.0	41.0	33.0	41.0
60-61	37.241371815273986	39.0	36.0	41.0	33.0	41.0
62-63	36.99499052958088	39.0	35.0	41.0	32.5	41.0
64-65	36.70616945643704	39.0	35.0	41.0	32.0	41.0
66-67	36.44350168214618	38.0	35.0	40.5	32.0	41.0
68-69	36.12507824273305	37.0	35.0	40.0	31.5	41.0
70-71	35.83052536922635	37.0	35.0	39.5	31.0	41.0
72-73	35.49652848074193	36.0	35.0	39.0	31.0	41.0
74-75	35.15121257365075	36.0	35.0	39.0	31.0	40.5
76-77	34.833423011666824	35.0	35.0	37.5	31.0	39.5
78-79	34.532565815832186	35.0	35.0	37.0	31.0	39.0
80-81	34.24911866843546	35.0	35.0	37.0	31.0	39.0
82-83	33.98895117769588	35.0	34.0	36.0	30.0	38.0
84-85	33.772989814992954	35.0	34.0	36.0	30.0	37.0
86-87	33.58583764918378	35.0	34.0	35.5	30.0	37.0
88-89	33.42134132722127	35.0	34.0	35.0	30.0	36.0
90-91	33.331856415052485	35.0	34.0	35.0	30.0	36.0
92-93	33.251634436015536	35.0	34.0	35.0	30.0	36.0
94-95	33.167492481536264	35.0	34.0	35.0	30.0	36.0
96-97	33.120359872055474	35.0	34.0	35.0	30.0	35.5
98-99	33.06223592472749	35.0	34.0	35.0	29.0	35.0
100	32.528589517235076	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.26955669619317746
1101	2	0.3052254012168376
1101	3	0.30449712122676686
1101	4	0.2102049886999211
1101	5	0.12863624114685024
1101	6	0.15486901309487422
1101	7	0.21509372168564767
1101	8	0.18396640259427954
1101	9	0.1983434061641347
1101	10-11	0.2403315181567649
1101	12-13	0.2320159385041336
1101	14-15	0.34247404414950466
1101	16-17	0.36374922833704204
1101	18-19	0.39369264188841413
1101	20-21	0.3311353346771213
1101	22-23	0.3084161253952047
1101	24-25	0.31482507948076943
1101	26-27	0.3391264526667399
1101	28-29	0.3525653066677279
1101	30-31	0.4005323968168284
1101	32-33	0.3226894597903538
1101	34-35	0.3552479039890315
1101	36-37	0.41399837910702786
1101	38-39	0.4337052582188008
1101	40-41	0.32958507525769676
1101	42-43	0.3600208583615512
1101	44-45	0.41449503265539533
1101	46-47	0.4289697485951294
1101	48-49	0.47804168465916064
1101	50-51	0.4371837932190772
1101	52-53	0.4554508769242318
1101	54-55	0.4054662042593762
1101	56-57	0.4275223572179172
1101	58-59	0.4138352507484129
1101	60-61	0.3169875418460322
1101	62-63	0.40202068778569355
1101	64-65	0.3795879768506012
1101	66-67	0.3808015238880813
1101	68-69	0.3791506888840104
1101	70-71	0.3566020257958087
1101	72-73	0.35662684049534477
1101	74-75	0.36465587217133333
1101	76-77	0.3227326055921864
1101	78-79	0.32302458572106474
1101	80-81	0.3225403467231587
1101	82-83	0.2849866253184814
1101	84-85	0.22439396223047225
1101	86-87	0.3291329850189797
1101	88-89	0.20401746697792333
1101	90-91	0.28278704995782533
1101	92-93	0.31456730478331707
1101	94-95	0.27843213227598085
1101	96-97	0.21805869358285435
1101	98-99	0.24566722524088647
1101	100	0.13241900146339702
1102	1	0.33115282367627685
1102	2	0.3012504734404615
1102	3	0.2901156488058376
1102	4	0.22780614744918637
1102	5	0.15983493445160946
1102	6	0.1941248503598203
1102	7	0.2058771735216851
1102	8	0.21642842766856774
1102	9	0.31555008827573516
1102	10-11	0.3081018134179061
1102	12-13	0.2693196868939367
1102	14-15	0.39199341793680276
1102	16-17	0.34033928562302407
1102	18-19	0.43983230157820685
1102	20-21	0.40900366655179
1102	22-23	0.40925555161852856
1102	24-25	0.4021794300817021
1102	26-27	0.4297517208877579
1102	28-29	0.4438998417461235
1102	30-31	0.43356873802877516
1102	32-33	0.42172096862901753
1102	34-35	0.4713677416069544
1102	36-37	0.5190106780640633
1102	38-39	0.48016092320182935
1102	40-41	0.46431992553134194
1102	42-43	0.515664581912155
1102	44-45	0.6097124946235937
1102	46-47	0.5979502829448293
1102	48-49	0.5583399912430735
1102	50-51	0.508050888648711
1102	52-53	0.5628299409143125
1102	54-55	0.5376091992829117
1102	56-57	0.5716178840582842
1102	58-59	0.5376948482264439
1102	60-61	0.5387704321629769
1102	62-63	0.49409947694510237
1102	64-65	0.49134552814479093
1102	66-67	0.4633200541902909
1102	68-69	0.44364911859535994
1102	70-71	0.43182994256689256
1102	72-73	0.44995073532133745
1102	74-75	0.4514314255123679
1102	76-77	0.4648860399070358
1102	78-79	0.4666459322707297
1102	80-81	0.3922191691807484
1102	82-83	0.3790096613890981
1102	84-85	0.3748512607549088
1102	86-87	0.3834941682972257
1102	88-89	0.4101729174530746
1102	90-91	0.37748794194244084
1102	92-93	0.3871965584455168
1102	94-95	0.4226575685735554
1102	96-97	0.3653737344006984
1102	98-99	0.3708858523008658
1102	100	0.43091767039774
1103	1	0.2728190491110851
1103	2	0.1931463409054217
1103	3	0.2034460209200688
1103	4	0.14526585390032665
1103	5	0.13391578322394082
1103	6	0.12663757188303748
1103	7	0.16073464257827652
1103	8	0.15737602685933894
1103	9	0.1808981555184488
1103	10-11	0.19321691194086554
1103	12-13	0.2115038135451357
1103	14-15	0.26290845506752447
1103	16-17	0.22296055869639986
1103	18-19	0.24930402871746793
1103	20-21	0.23560614263716673
1103	22-23	0.24367366270237767
1103	24-25	0.26887158037112613
1103	26-27	0.26031302569918324
1103	28-29	0.2775273532988791
1103	30-31	0.25082707250136593
1103	32-33	0.2490756102548346
1103	34-35	0.31968818898371154
1103	36-37	0.26820330888477883
1103	38-39	0.27839064422808946
1103	40-41	0.24868353100229967
1103	42-43	0.2703665954787198
1103	44-45	0.21106182137754104
1103	46-47	0.29834264163756075
1103	48-49	0.26260064809912365
1103	50-51	0.24470607931449706
1103	52-53	0.2584584000798813
1103	54-55	0.25714755895935326
1103	56-57	0.25925824473682013
1103	58-59	0.27474467893812005
1103	60-61	0.27368694229873824
1103	62-63	0.2848794732915323
1103	64-65	0.30519579340317193
1103	66-67	0.23302660407437514
1103	68-69	0.25014640387023235
1103	70-71	0.22737873313476342
1103	72-73	0.1840748157359684
1103	74-75	0.22491995669037834
1103	76-77	0.22158602511228764
1103	78-79	0.21988570912714778
1103	80-81	0.20692655924973735
1103	82-83	0.2165322003921517
1103	84-85	0.18818822155326131
1103	86-87	0.21194351893728935
1103	88-89	0.2225853769101107
1103	90-91	0.20107400948036513
1103	92-93	0.16659349635882847
1103	94-95	0.1456352383824111
1103	96-97	0.19407569382768486
1103	98-99	0.13142187324982046
1103	100	0.15891014601832865
1104	1	0.16129627943745106
1104	2	0.14882484403950968
1104	3	0.14426446580940677
1104	4	0.14212207326541915
1104	5	0.07745855648528277
1104	6	0.08894105920164463
1104	7	0.1017712589466413
1104	8	0.1447615708953336
1104	9	0.13271025341629183
1104	10-11	0.14835542111493538
1104	12-13	0.1248331212627889
1104	14-15	0.20432499478028632
1104	16-17	0.22058135570887316
1104	18-19	0.22616694336644372
1104	20-21	0.20672762849284965
1104	22-23	0.2413890397879257
1104	24-25	0.18819243210631953
1104	26-27	0.2473211487159901
1104	28-29	0.2519102290742268
1104	30-31	0.20476911833677036
1104	32-33	0.20844602054076233
1104	34-35	0.19064136219012795
1104	36-37	0.2680531693723225
1104	38-39	0.1977192766307283
1104	40-41	0.18129669514091518
1104	42-43	0.20246021434612516
1104	44-45	0.1690826182023457
1104	46-47	0.1857078230021827
1104	48-49	0.256847215964612
1104	50-51	0.26964835770975526
1104	52-53	0.23841802037079418
1104	54-55	0.28773347997064036
1104	56-57	0.27511313364686885
1104	58-59	0.23430800285802178
1104	60-61	0.2347599502953912
1104	62-63	0.24743991812988497
1104	64-65	0.24166386772161275
1104	66-67	0.15821142034036484
1104	68-69	0.29664840346266885
1104	70-71	0.18714658304153886
1104	72-73	0.2011073223479798
1104	74-75	0.22003627805962367
1104	76-77	0.1729899312106582
1104	78-79	0.1357859605013516
1104	80-81	0.15330173005298775
1104	82-83	0.2465619796260725
1104	84-85	0.16063981055322785
1104	86-87	0.17229671744846797
1104	88-89	0.21857778579379783
1104	90-91	0.22045612126455438
1104	92-93	0.2500342732807539
1104	94-95	0.13968275409740727
1104	96-97	0.0988978778164622
1104	98-99	0.13341618734136773
1104	100	0.13595780264551394
1105	1	0.1440237744803099
1105	2	0.17932002864144891
1105	3	0.18773110737605947
1105	4	0.10932627636103831
1105	5	0.06993010924393417
1105	6	0.13706010448425587
1105	7	0.10312016230067655
1105	8	0.12505294025030622
1105	9	0.08519645675178822
1105	10-11	0.0955820807144363
1105	12-13	0.13113961159739063
1105	14-15	0.16043957457419822
1105	16-17	0.18520646538237884
1105	18-19	0.18815632881880617
1105	20-21	0.13577446650273117
1105	22-23	0.22952305162276332
1105	24-25	0.19928060904185685
1105	26-27	0.16492400370932359
1105	28-29	0.23221350313547617
1105	30-31	0.23214170960859803
1105	32-33	0.20612115379002915
1105	34-35	0.22165189818895925
1105	36-37	0.16648978103925316
1105	38-39	0.1685594217924944
1105	40-41	0.19373277410004164
1105	42-43	0.20565723990520013
1105	44-45	0.1978233606243336
1105	46-47	0.31468474589595274
1105	48-49	0.2795972209156332
1105	50-51	0.2901800819718403
1105	52-53	0.19958230954524225
1105	54-55	0.19133918185765708
1105	56-57	0.18536755835917518
1105	58-59	0.13686620185174547
1105	60-61	0.12135048914689861
1105	62-63	0.19411095059724914
1105	64-65	0.17858394441907421
1105	66-67	0.14942240934108497
1105	68-69	0.1956334104308155
1105	70-71	0.15016110834082497
1105	72-73	0.137899896303459
1105	74-75	0.15977231845641882
1105	76-77	0.13523699236146314
1105	78-79	0.12944264345855316
1105	80-81	0.1178081264405435
1105	82-83	0.16123861331336542
1105	84-85	0.16145122198187778
1105	86-87	0.12297585312673931
1105	88-89	0.12645182554572187
1105	90-91	0.07567408753573801
1105	92-93	0.12666891740013142
1105	94-95	0.16930322093546124
1105	96-97	0.13229525179402657
1105	98-99	0.13607727860016894
1105	100	0.0915309964512403
1106	1	0.15565617684732302
1106	2	0.14565901761077527
1106	3	0.1487237877413179
1106	4	0.10193715987489327
1106	5	0.04100565995358352
1106	6	0.1062302519191789
1106	7	0.10126197098179546
1106	8	0.0825405782896027
1106	9	0.09890997104053412
1106	10-11	0.10860534284680057
1106	12-13	0.13129950869314655
1106	14-15	0.14906572447483057
1106	16-17	0.12771544233491738
1106	18-19	0.1647654770705742
1106	20-21	0.15504340272828188
1106	22-23	0.16853088346268663
1106	24-25	0.14953156731094452
1106	26-27	0.13827574970543566
1106	28-29	0.10260666292478504
1106	30-31	0.195718465113913
1106	32-33	0.1459050878286945
1106	34-35	0.1415029221984625
1106	36-37	0.13058553401204165
1106	38-39	0.16084538906302726
1106	40-41	0.1775125721932156
1106	42-43	0.11663865175646748
1106	44-45	0.0341759208039889
1106	46-47	0.1183230951878258
1106	48-49	0.11534081367420868
1106	50-51	0.1489487451697613
1106	52-53	0.11841148421789427
1106	54-55	0.08351598261519655
1106	56-57	-0.005304940320229434
1106	58-59	0.058207408892208434
1106	60-61	0.06661501862630814
1106	62-63	0.045524681437427716
1106	64-65	0.05258547836223926
1106	66-67	0.03802502007316377
1106	68-69	0.10984349650800596
1106	70-71	0.08105716899304127
1106	72-73	0.098666315597292
1106	74-75	0.05395629543123448
1106	76-77	0.06594843645048343
1106	78-79	0.04842759531572227
1106	80-81	-0.00924648443049847
1106	82-83	-0.015096594245527228
1106	84-85	0.07443791711824588
1106	86-87	0.03714006343550835
1106	88-89	0.04327393535005086
1106	90-91	0.022967394929217733
1106	92-93	0.0024750838160443323
1106	94-95	0.02910698399557532
1106	96-97	-0.02954755555358446
1106	98-99	-0.012311680215859155
1106	100	0.004367036454908657
1107	1	0.14909926235598192
1107	2	0.15158684997430782
1107	3	0.1899852894372387
1107	4	0.08974479139442337
1107	5	0.015217591359039773
1107	6	0.040115531355105816
1107	7	0.060752098049761116
1107	8	0.04320169871679269
1107	9	0.0763081854535983
1107	10-11	0.14523040285165933
1107	12-13	0.1398688940488313
1107	14-15	0.16182516183425832
1107	16-17	0.12670709281472625
1107	18-19	0.17499456246817147
1107	20-21	0.1298904766472333
1107	22-23	0.14795929184473522
1107	24-25	0.14286715144332618
1107	26-27	0.20159162912801065
1107	28-29	0.22152474877874795
1107	30-31	0.2318956203350453
1107	32-33	0.25183129234444124
1107	34-35	0.1873157735422737
1107	36-37	0.20931952789922548
1107	38-39	0.1798193995923114
1107	40-41	0.23034793068194404
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2216	26-27	-0.3634020826068607
2216	28-29	-0.33444576585192465
2216	30-31	-0.39296134158706764
2216	32-33	-0.4067053047800897
2216	34-35	-0.37693233496964496
2216	36-37	-0.3621299271231493
2216	38-39	-0.3573570016987446
2216	40-41	-0.3368942951764282
2216	42-43	-0.30665177046814307
2216	44-45	-0.34187695380242644
2216	46-47	-0.36102444100598774
2216	48-49	-0.38459748667966664
2216	50-51	-0.4457945444961524
2216	52-53	-0.4528759883655695
2216	54-55	-0.4982917772099782
2216	56-57	-0.4121777833205016
2216	58-59	-0.48276662948895677
2216	60-61	-0.37014632714248563
2216	62-63	-0.3731094078160808
2216	64-65	-0.3571598812272754
2216	66-67	-0.413476570185459
2216	68-69	-0.3357543332567019
2216	70-71	-0.37627205235742167
2216	72-73	-0.36784769420300023
2216	74-75	-0.2963106052960285
2216	76-77	-0.3239480474658123
2216	78-79	-0.3440717180269317
2216	80-81	-0.23671566446211045
2216	82-83	-0.23991467980503245
2216	84-85	-0.21325409815142393
2216	86-87	-0.27571893894091914
2216	88-89	-0.3361828689267341
2216	90-91	-0.3230845243278395
2216	92-93	-0.31467552701045776
2216	94-95	-0.33462685965353955
2216	96-97	-0.29773302867045004
2216	98-99	-0.3195102376383261
2216	100	-0.48353784259388277
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	15.0
8	53.0
9	107.0
10	186.0
11	192.0
12	171.0
13	178.0
14	250.0
15	436.0
16	802.0
17	1529.0
18	2659.0
19	4359.0
20	6377.0
21	9481.0
22	13903.0
23	17559.0
24	18198.0
25	16184.0
26	13979.0
27	14360.0
28	16801.0
29	20192.0
30	25336.0
31	32347.0
32	41810.0
33	56004.0
34	80996.0
35	128139.0
36	233108.0
37	576407.0
38	918784.0
39	331345.0
40	1060.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.657480118351124	18.967187875629897	13.437838369346702	23.937493636672276
2	15.470836555463777	20.630326200995853	39.98495994743343	23.913877296106946
3	18.128957634775393	25.854160069802244	30.721542772068883	25.295339523353483
4	12.179582217676801	17.592101906587175	38.49163107598129	31.736684799754734
5	12.664529095365884	38.133149006532754	35.302532170060836	13.89978972804052
6	30.134349129663075	38.33067190049532	17.820412318853204	13.714566650988408
7	26.18436460270855	33.5136966018337	22.45580757982507	17.84613121563269
8	25.55611857204738	35.40698027760541	20.312490927326873	18.72441022302034
9	26.14540199829134	15.161612615147948	20.256593583341044	38.43639180321967
10-11	24.369867770265014	26.154227894710154	28.83480360638515	20.641100728639685
12-13	25.50407288022678	24.131162111200876	28.670479350692734	21.694285657879607
14-15	22.543487862650473	25.236450797369418	26.790389218161064	25.429672121819046
16-17	21.616013892270644	28.24093303660773	27.33207861086584	22.81097446025579
18-19	21.554697138203085	26.903558113689158	29.949247224584614	21.592497523523143
20-21	22.381234595810717	26.041775135514282	30.125068371664693	21.451921897010305
22-23	22.189694870429093	25.981130307947677	29.841547387523537	21.987627434099693
24-25	21.927571906862017	26.105995144982767	30.017357596290335	21.949075351864877
26-27	21.834454828636318	26.314855338525383	29.780935831474924	22.069754001363368
28-29	21.753025678346148	26.420689775307217	29.85625003629072	21.970034510055918
30-31	22.03868458326414	26.225088578459438	29.779704510078766	21.95652232819766
32-33	21.969118948989788	26.38169031695136	29.569997990916153	22.079192743142695
34-35	22.140930048277873	26.384691212645034	29.64363640023544	21.830742338841652
36-37	22.164902958979813	26.242631002570754	29.528055126623464	22.06441091182597
38-39	22.141715464278292	26.390214180449806	29.48049947179739	21.98757088347452
40-41	22.190391377451903	26.19842614419263	29.559568508715785	22.051613969639682
42-43	22.1547370886294	26.27334963041244	29.279154255144128	22.292759025814032
44-45	22.270345535583637	26.47397082949134	29.09926241457612	22.156421220348903
46-47	22.417601389852344	26.536506075958776	28.598135869834508	22.447756664354376
48-49	22.519664403978734	26.73594410526599	28.31424490798439	22.430146582770888
50-51	22.47166306208868	26.97257256156783	28.151844412297656	22.403919964045834
52-53	22.500842441475722	27.04342623706313	28.03376191878071	22.421969402680446
54-55	22.37550967319366	26.931764470886353	28.07302983463227	22.619696021287723
56-57	22.525421619423497	26.97677200353736	27.95742317867938	22.540383198359763
58-59	22.460716952631948	26.887001164042506	28.166325299362544	22.485956583963002
60-61	22.33889872035648	26.900095364052806	28.2524571195643	22.508548796026414
62-63	22.376408141429447	26.85627308837095	28.240307577843836	22.52701119235577
64-65	22.255462827686433	26.746002804288967	28.218390003662147	22.78014436436246
66-67	22.302854668618004	26.890322990241327	28.082957134968222	22.723865206172448
68-69	22.33495876545872	26.733884056293515	28.20221193743212	22.728945240815644
70-71	22.297200389970776	26.68113927674241	28.113393467699183	22.908266865587635
72-73	22.412855669861642	26.723022455148644	27.995450553708878	22.868671321280836
74-75	22.462852506333192	26.974767112003022	27.804972561334402	22.757407820329387
76-77	22.51332786082777	26.7470419146053	27.760874862792186	22.97875536177475
78-79	22.419954724366775	26.903988250632526	27.745222367773163	22.930834657227532
80-81	22.44791703965441	27.00192563290496	27.631138620214056	22.91901870722657
82-83	22.582260997746296	26.872371097146207	27.76193027373493	22.78343763137256
84-85	22.554348509825303	26.977733364378842	27.53680412045889	22.931114005336966
86-87	22.407275793111616	27.04443453466217	27.655008736866638	22.893280935359574
88-89	22.509148171933376	26.72158473086587	27.58840136058372	23.180865736617037
90-91	22.496319639903426	26.83790583155622	27.544713036429663	23.121061492110695
92-93	22.57910112890183	26.818047564613885	27.643230169701084	22.959621136783202
94-95	22.3682710510735	26.758748913456454	27.712333011975154	23.16064702349489
96-97	22.457296359673496	26.594255367701408	27.700650931357718	23.247797341267376
98-99	22.376282811344133	26.759683674816348	27.52274134040648	23.341292173433036
100	22.450591997943704	26.597093779008596	27.552930647245404	23.399383575802293
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	368.0
1	259.0
2	427.0
3	671.0
4	424.0
5	308.5
6	700.5
7	1812.0
8	3479.0
9	4219.5
10	3047.0
11	1772.0
12	1595.0
13	1735.0
14	1967.5
15	2227.5
16	2511.0
17	2893.0
18	3337.5
19	3686.5
20	4149.5
21	4944.5
22	6127.0
23	7517.5
24	9218.0
25	11459.5
26	14559.5
27	18376.0
28	22044.5
29	26422.0
30	31156.0
31	36083.5
32	42555.5
33	47444.0
34	50868.5
35	55110.0
36	59969.0
37	65906.5
38	70293.5
39	72638.0
40	74921.0
41	76828.5
42	78819.0
43	83036.0
44	88304.5
45	94455.5
46	99763.5
47	101888.5
48	103154.5
49	102401.5
50	99378.5
51	97203.5
52	94397.5
53	90999.5
54	87858.0
55	81327.0
56	74499.0
57	67572.5
58	59708.0
59	52427.0
60	43650.5
61	35520.5
62	28308.5
63	20985.5
64	14655.5
65	10200.0
66	7533.0
67	5548.0
68	3949.0
69	2791.5
70	1999.5
71	1427.0
72	1036.5
73	805.5
74	583.5
75	411.0
76	285.5
77	177.0
78	123.5
79	86.5
80	57.0
81	38.0
82	30.5
83	21.5
84	12.5
85	9.5
86	7.0
87	5.0
88	3.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005651670513802657
2	0.08512344835515098
3	3.483906481111227E-4
4	0.0
5	0.036271337475569106
6	0.0043355280653828605
7	7.742014402469393E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.258107921358166E-4
24-25	0.0
26-27	0.0
28-29	7.742014402469393E-5
30-31	1.5484028804938786E-4
32-33	0.0015484028804938787
34-35	0.0012580773404012764
36-37	6.967812962222454E-4
38-39	6.967812962222454E-4
40-41	0.0012967874124136234
42-43	8.516215842716332E-4
44-45	5.032309361605106E-4
46-47	4.258107921358166E-4
48-49	8.709766202778067E-4
50-51	6.967812962222454E-4
52-53	0.001606467988512399
54-55	0.0013161424484197967
56-57	9.096866922901537E-4
58-59	0.0026322848968395934
60-61	0.0018774384925988278
62-63	0.0010064618723210211
64-65	0.004683918713493982
66-67	0.004548433461450768
68-69	0.0037355219491914823
70-71	0.0014129176284506642
72-73	0.0052452147576730135
74-75	0.0035226165531235735
76-77	0.0035032615171174003
78-79	7.354913682345924E-4
80-81	1.9355036006173483E-5
82-83	1.9355036006173484E-4
84-85	9.677518003086742E-5
86-87	3.8710072012346966E-5
88-89	1.5484028804938786E-4
90-91	0.0
92-93	0.0
94-95	1.9355036006173483E-5
96-97	3.290356121049492E-4
98-99	6.193611521975515E-4
100	8.129115122592864E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2583307.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.038692111276475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.47235409217731	45.581287542152715
2	15.386048664540514	19.090606718170907
3	5.378542715380755	10.010332665805668
4	2.35702750455326	5.849076146111599
5	1.2353278813153223	3.831906304269838
6	0.6696358233582351	2.4925998403201604
7	0.42683825728860914	1.853634105766928
8	0.2640788015906213	1.310648277199634
9	0.1813233268785288	1.0124155843928453
>10	0.6060384602184524	5.823568392480476
>50	0.015699218154519513	0.6721908527673508
>100	0.005789622116051705	0.6610013565443987
>500	8.637549518388454E-4	0.3705487632781219
>1k	2.5912648555165155E-4	0.4257425776203415
>5k	8.637549518388386E-5	0.4764887350786755
>10k+	8.637549518388386E-5	0.5379521380403535
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10039	0.3886104129319512	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8892	0.3442099603337892	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4957	0.1918858269652039	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5484028804938786E-4
2	0.0	0.0	0.0	3.8710072012346966E-5	1.5484028804938786E-4
3	0.0	0.0	0.0	3.8710072012346966E-5	1.5484028804938786E-4
4	0.0	0.0	0.0	3.8710072012346966E-5	1.5484028804938786E-4
5	0.0	0.0	0.0	3.8710072012346966E-5	1.5484028804938786E-4
6	0.0	0.0	0.0	3.8710072012346966E-5	1.935503600617348E-4
7	0.0	0.0	0.0	1.1613021603704089E-4	1.935503600617348E-4
8	0.0	0.0	0.0	1.5484028804938786E-4	2.3226043207408178E-4
9	0.0	0.0	0.0	3.0968057609877573E-4	2.3226043207408178E-4
10-11	0.0	0.0	0.0	3.290356121049492E-4	2.709705040864288E-4
12-13	0.0	0.0	0.0	3.483906481111227E-4	8.903316562839801E-4
14-15	0.0	0.0	0.0	3.871007201234696E-4	0.0010645269803395414
16-17	0.0	0.0	0.0	4.6452086414816357E-4	0.001238722304395103
18-19	0.0	0.0	0.0	4.6452086414816357E-4	0.0013354974844259704
20-21	0.0	0.0	0.0	4.8387590015433707E-4	0.0014903377724753582
22-23	0.0	0.0	0.0	5.806510801852045E-4	0.0015871129525062255
24-25	0.0	0.0	0.0	0.0010064618723210211	0.0015871129525062255
26-27	0.0	0.0	0.0	0.0015484028804938785	0.001761308276561787
28-29	0.0	0.0	0.0	0.003754876985197655	0.0018193733845803073
30-31	0.0	0.0	0.0	0.009600097859062047	0.0018387284205864808
32-33	3.8710072012346966E-5	0.0	0.0	0.02388411443161808	0.0020129237446420423
34-35	3.8710072012346966E-5	0.0	0.0	0.04720693281905712	0.0020903438886667363
36-37	3.8710072012346966E-5	0.0	0.0	0.08334278504258302	0.0021290539606790833
38-39	3.8710072012346966E-5	0.0	0.0	0.1363949387355045	0.002245184176716124
40-41	3.8710072012346966E-5	0.0	0.0	0.19124711077700018	0.002283894248728471
42-43	3.8710072012346966E-5	0.0	0.0	0.2453057263422427	0.002322604320740818
44-45	3.8710072012346966E-5	0.0	0.0	0.31304835236384987	0.002400024464765512
46-47	3.8710072012346966E-5	0.0	0.0	0.38992655538037096	0.0025548647528149
48-49	3.8710072012346966E-5	0.0	0.0	0.47195319797453417	0.0026516399328457667
50-51	3.8710072012346966E-5	0.0	0.0	0.559844416478568	0.0029032554009260224
52-53	3.8710072012346966E-5	0.0	0.0	0.6421226745408115	0.003174225905012451
54-55	3.8710072012346966E-5	0.0	0.0	0.7291816264965798	0.003290356121049492
56-57	3.8710072012346966E-5	0.0	0.0	0.8301181392687744	0.0033484212290680127
58-59	3.8710072012346966E-5	0.0	0.0	0.9410418506201548	0.003367776265074186
60-61	3.8710072012346966E-5	0.0	0.0	1.060559197958276	0.0033871313010803593
62-63	3.8710072012346966E-5	0.0	0.0	1.177599100687607	0.0037935870572100025
64-65	3.8710072012346966E-5	0.0	0.0	1.2907099311076848	0.003812942093216176
66-67	3.8710072012346966E-5	0.0	0.0	1.4204660924930719	0.0038322971292223495
68-69	3.8710072012346966E-5	0.0	0.0	1.5660353182955027	0.0038322971292223495
70-71	3.8710072012346966E-5	0.0	0.0	1.7201207599406496	0.0038710072012346965
72-73	5.8065108018520446E-5	0.0	0.0	1.8744191069818648	0.004122622669314952
74-75	7.742014402469393E-5	0.0	0.0	2.0281755130149066	0.004354883101389033
76-77	7.742014402469393E-5	0.0	0.0	2.1954610892162645	0.004374238137395207
78-79	7.742014402469393E-5	0.0	0.0	2.378850055374758	0.004451658281419901
80-81	7.742014402469393E-5	0.0	0.0	2.578110151058314	0.004451658281419901
82-83	7.742014402469393E-5	0.0	0.0	2.783854183803938	0.004587143533463116
84-85	7.742014402469393E-5	0.0	0.0	2.9827078237313644	0.004606498569469289
86-87	7.742014402469393E-5	0.0	0.0	3.1853550507160007	0.004703273749500156
88	7.742014402469393E-5	0.0	0.0	3.345595393811111	0.004800048929531024
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4895	0.0	59.1232	1
GTATCAA	9600	0.0	45.734703	1
ATCAACG	12000	0.0	36.204655	3
TCAACGC	12035	0.0	36.099365	4
CAACGCA	12125	0.0	35.792637	5
TATCAAC	12395	0.0	35.344498	2
AACGCAG	12695	0.0	34.20935	6
ACGCAGA	14230	0.0	30.351078	7
CGCAGAG	14490	0.0	29.709177	8
GCAGAGT	15660	0.0	27.099388	9
GTACATG	7815	0.0	24.447392	1
ACATGGG	7435	0.0	23.525328	3
TACATGG	7970	0.0	23.203466	2
GAGTACT	11340	0.0	23.166597	12-13
TGGTATC	1710	0.0	22.84024	2
CAGAGTA	15220	0.0	22.571802	10-11
AGAGTAC	14525	0.0	22.470858	10-11
GTGGTAT	1820	0.0	21.97774	1
CATGGGG	5365	0.0	21.38424	4
GTACTTT	12265	0.0	21.17036	14-15
>>END_MODULE