FastQCFastQC Report
Mon 16 Jan 2017
ERR1146491_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146491_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3280820
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54800.040.584231
GGTATCA31950.039.7346731
ATCAACG63400.034.4689873
CAACGCA63300.034.225425
TCAACGC66600.032.6711924
TATCAAC68300.032.3401452
AACGCAG69550.031.4852586
ACGCAGA77600.027.7934827
CGCAGAG79750.026.9292268
TACATGG101750.023.5559482
GTACATG106750.023.0361841
ACATGGG99300.022.622653
GTACTAG9700.022.2978421
GCAGAGT94450.022.1409599
CATGGGG71700.020.384474
ATTGCGT4550.019.6227876
AGAGTAC77800.018.63419210-11
CTAGTAC10200.017.508473
TAGTACT12750.017.3239084
CAGAGTA88050.016.94530510-11
Produced by FastQC (version 0.11.5)