##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146491_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3280820
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.313183289543467	31.0	31.0	34.0	30.0	34.0
2	31.542593010284016	31.0	31.0	34.0	30.0	34.0
3	31.746673087825606	33.0	31.0	34.0	30.0	34.0
4	35.34754908833767	37.0	35.0	37.0	33.0	37.0
5	35.46328143573863	37.0	35.0	37.0	33.0	37.0
6	35.468710566260874	37.0	35.0	37.0	33.0	37.0
7	35.39787309270243	37.0	35.0	37.0	33.0	37.0
8	35.37925213818497	37.0	35.0	37.0	33.0	37.0
9	37.06274407008004	39.0	38.0	39.0	34.0	39.0
10-11	37.04923692857274	39.0	38.0	39.0	34.0	39.0
12-13	37.022453532958224	39.0	38.0	39.0	34.0	39.0
14-15	38.47208838034393	41.0	39.0	41.0	34.0	41.0
16-17	38.483750556263374	41.0	39.0	41.0	34.0	41.0
18-19	38.50087188568712	41.0	39.0	41.0	34.0	41.0
20-21	38.58303777714108	41.0	39.0	41.0	34.0	41.0
22-23	38.600703787467765	41.0	39.0	41.0	34.0	41.0
24-25	38.654317060978656	41.0	39.0	41.0	34.0	41.0
26-27	38.68092976755811	41.0	39.0	41.0	34.0	41.0
28-29	38.642256051840704	41.0	39.0	41.0	34.0	41.0
30-31	38.62379435628898	41.0	39.0	41.0	34.0	41.0
32-33	38.63531601855634	41.0	39.0	41.0	34.0	41.0
34-35	38.58735575252528	41.0	39.0	41.0	34.0	41.0
36-37	38.500536908455814	40.0	38.5	41.0	33.0	41.0
38-39	38.467164001682505	40.0	38.0	41.0	33.0	41.0
40-41	38.40938987814022	40.0	38.0	41.0	33.0	41.0
42-43	38.30734633414817	40.0	38.0	41.0	33.0	41.0
44-45	38.197482031931045	40.0	38.0	41.0	33.0	41.0
46-47	38.105047823410004	40.0	38.0	41.0	33.0	41.0
48-49	38.00389673922983	40.0	38.0	41.0	33.0	41.0
50-51	37.90135926384258	40.0	37.5	41.0	33.0	41.0
52-53	37.79798937460757	40.0	37.0	41.0	32.0	41.0
54-55	37.63067251479813	40.0	37.0	41.0	32.0	41.0
56-57	37.51935019293957	40.0	36.0	41.0	32.0	41.0
58-59	37.317221456830914	39.5	36.0	41.0	31.5	41.0
60-61	37.14560460494633	39.0	35.5	41.0	31.0	41.0
62-63	36.92107018976963	39.0	35.0	41.0	31.0	41.0
64-65	36.67644978999152	39.0	35.0	41.0	31.0	41.0
66-67	36.407478618150336	38.0	35.0	40.0	31.0	41.0
68-69	36.103472302656044	37.0	35.0	40.0	31.0	41.0
70-71	35.8099638200206	37.0	35.0	39.5	30.0	41.0
72-73	35.46817000018288	36.0	35.0	39.0	30.0	41.0
74-75	35.12880011704391	36.0	35.0	39.0	30.0	40.5
76-77	34.794981285166514	35.0	34.0	37.5	30.0	39.5
78-79	34.490830798397965	35.0	34.0	37.0	29.5	39.0
80-81	34.18422558994398	35.0	34.0	37.0	29.0	39.0
82-83	33.92005428520919	35.0	34.0	36.0	29.0	38.0
84-85	33.685629659658254	35.0	34.0	36.0	29.0	37.0
86-87	33.48056522454752	35.0	34.0	35.5	29.0	37.0
88-89	33.31859687517145	35.0	34.0	35.0	29.0	36.5
90-91	33.157976664370494	35.0	34.0	35.0	29.0	36.0
92-93	33.06573128059449	35.0	34.0	35.0	29.0	36.0
94-95	32.965781268097615	35.0	34.0	35.0	28.0	36.0
96-97	32.89563188471175	35.0	34.0	35.0	28.0	35.5
98-99	32.82433355075865	35.0	34.0	35.0	28.0	35.0
100	31.958831938356873	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.07560951405414329
1101	2	0.11575262553602172
1101	3	0.2408150385508101
1101	4	0.19406445945323725
1101	5	0.2185829316121044
1101	6	0.27973865794204755
1101	7	0.2567472250970937
1101	8	0.26918968295834844
1101	9	0.38116243968691776
1101	10-11	0.38345326099410215
1101	12-13	0.39535664351181765
1101	14-15	0.472803426793277
1101	16-17	0.48930291362945866
1101	18-19	0.421867385409584
1101	20-21	0.4310094661722701
1101	22-23	0.38306689393517246
1101	24-25	0.37937861801978556
1101	26-27	0.4033548520897483
1101	28-29	0.4308493943789884
1101	30-31	0.44067603210079653
1101	32-33	0.3807986666319749
1101	34-35	0.43878465752183615
1101	36-37	0.3902210233604819
1101	38-39	0.34804732532744254
1101	40-41	0.35645214816887716
1101	42-43	0.33375879328646363
1101	44-45	0.3329777985051763
1101	46-47	0.29345335413074025
1101	48-49	0.3000550302687799
1101	50-51	0.3378065876392071
1101	52-53	0.2507339720865218
1101	54-55	0.3623399620160228
1101	56-57	0.22838114296747136
1101	58-59	0.31699475254340825
1101	60-61	0.27984665745799475
1101	62-63	0.2829144487883255
1101	64-65	0.25320891911894705
1101	66-67	0.21967616525204647
1101	68-69	0.23307677705634688
1101	70-71	0.2148899727204352
1101	72-73	0.17140620857347955
1101	74-75	0.1872569205894834
1101	76-77	0.15660074756025466
1101	78-79	0.19646923853092346
1101	80-81	0.14967154691644424
1101	82-83	0.20039644214702435
1101	84-85	0.1882910193490872
1101	86-87	0.20712532481274337
1101	88-89	0.16531038476092874
1101	90-91	0.21707070773158677
1101	92-93	0.23340270592966306
1101	94-95	0.24244025172774286
1101	96-97	0.23030442333724466
1101	98-99	0.22586841245622935
1101	100	0.2008222109844091
1102	1	0.2127430537034094
1102	2	0.17618818636466216
1102	3	0.2679656773366652
1102	4	0.25415445389857894
1102	5	0.26176209114346705
1102	6	0.28281451872231145
1102	7	0.22715026428944185
1102	8	0.32693398559258213
1102	9	0.41031112410289694
1102	10-11	0.3731197062423135
1102	12-13	0.33367760364880894
1102	14-15	0.5108861221086869
1102	16-17	0.5086401525028563
1102	18-19	0.38154898758332223
1102	20-21	0.4209215675230453
1102	22-23	0.4202802338214653
1102	24-25	0.4203187953593357
1102	26-27	0.37894914627680976
1102	28-29	0.44703917943670035
1102	30-31	0.43595153170019074
1102	32-33	0.38821450521919587
1102	34-35	0.4336883532903144
1102	36-37	0.4262089624537708
1102	38-39	0.36976137298488254
1102	40-41	0.38120302040402265
1102	42-43	0.32453977011304147
1102	44-45	0.2885446809588572
1102	46-47	0.2938439561329673
1102	48-49	0.2817640096192662
1102	50-51	0.3309861311332156
1102	52-53	0.2887998690360689
1102	54-55	0.27679068424976805
1102	56-57	0.2658121416799091
1102	58-59	0.2666094940213597
1102	60-61	0.25948783796394537
1102	62-63	0.329465600292302
1102	64-65	0.22977791848668971
1102	66-67	0.24885924632192058
1102	68-69	0.31356278737725773
1102	70-71	0.24825872660805715
1102	72-73	0.22632726585302976
1102	74-75	0.22035338096581825
1102	76-77	0.22944950083353888
1102	78-79	0.24508938876270747
1102	80-81	0.26009544487964575
1102	82-83	0.2477026402000817
1102	84-85	0.21110312018791433
1102	86-87	0.2399107754441019
1102	88-89	0.1586491927616862
1102	90-91	0.24184525766337117
1102	92-93	0.22033877678869374
1102	94-95	0.23344600668789894
1102	96-97	0.1981437119312517
1102	98-99	0.21564596689574245
1102	100	0.1625540937068557
1103	1	0.0772386920683914
1103	2	0.07059811061127164
1103	3	0.11990896018447472
1103	4	0.11707409288605675
1103	5	0.2171258228891091
1103	6	0.24724895363413424
1103	7	0.22294561215765896
1103	8	0.2608732665796012
1103	9	0.38314911107404725
1103	10-11	0.35337364409674876
1103	12-13	0.34846249421887876
1103	14-15	0.36454042425544486
1103	16-17	0.36903347567884737
1103	18-19	0.35284982870200565
1103	20-21	0.3283280743328234
1103	22-23	0.32560250786233524
1103	24-25	0.304048235935511
1103	26-27	0.2878337604064001
1103	28-29	0.2892731598616365
1103	30-31	0.30326439053315113
1103	32-33	0.30837092961557033
1103	34-35	0.31831973949204695
1103	36-37	0.34441350707163565
1103	38-39	0.23140956566322757
1103	40-41	0.2879566520320296
1103	42-43	0.29766060865473065
1103	44-45	0.20534835467207557
1103	46-47	0.1874461140253274
1103	48-49	0.2727104584802902
1103	50-51	0.27819093637822334
1103	52-53	0.2347315546034281
1103	54-55	0.31121239737750983
1103	56-57	0.2777792888543402
1103	58-59	0.2370205214361505
1103	60-61	0.2535901552446589
1103	62-63	0.19715538538158484
1103	64-65	0.19189641138407865
1103	66-67	0.22764051530419493
1103	68-69	0.18999879902055028
1103	70-71	0.13864252622552442
1103	72-73	0.18447485528202634
1103	74-75	0.17197765702476886
1103	76-77	0.15715590247209832
1103	78-79	0.20544347144719666
1103	80-81	0.18628091142315384
1103	82-83	0.17748292063991045
1103	84-85	0.15269539516586406
1103	86-87	0.1753987589848549
1103	88-89	0.11674736552694043
1103	90-91	0.21151503512209047
1103	92-93	0.21505223962401487
1103	94-95	0.19582266897033662
1103	96-97	0.1980960209287872
1103	98-99	0.1330394507150956
1103	100	0.11153277415538199
1104	1	0.1359585243641348
1104	2	0.10069995804796861
1104	3	0.18259909744454106
1104	4	0.15662986777910248
1104	5	0.18821884606737882
1104	6	0.11849191918073387
1104	7	0.1426464165556709
1104	8	0.1928106575823989
1104	9	0.24127684329854304
1104	10-11	0.32732046290657024
1104	12-13	0.27700700842894577
1104	14-15	0.37030164575777746
1104	16-17	0.4005822280672007
1104	18-19	0.2902158225731455
1104	20-21	0.37718633377868827
1104	22-23	0.3533401787427266
1104	24-25	0.32292941466702985
1104	26-27	0.3270096775946385
1104	28-29	0.3639039448416099
1104	30-31	0.3185359484999424
1104	32-33	0.33601495598497166
1104	34-35	0.3527478521855798
1104	36-37	0.31906724468248626
1104	38-39	0.3154206407244473
1104	40-41	0.2654191692187098
1104	42-43	0.295086635048591
1104	44-45	0.24628128741809263
1104	46-47	0.23468907512589965
1104	48-49	0.2650668989440561
1104	50-51	0.20162279374068248
1104	52-53	0.22763430102743598
1104	54-55	0.25902545069986616
1104	56-57	0.24699997756665226
1104	58-59	0.24461878703977646
1104	60-61	0.17972742198090685
1104	62-63	0.25378808437756106
1104	64-65	0.2322149583324915
1104	66-67	0.21756025334409657
1104	68-69	0.20002904314095815
1104	70-71	0.21435786469624674
1104	72-73	0.1707827495420915
1104	74-75	0.16030682776232652
1104	76-77	0.13761782277922663
1104	78-79	0.14747418462378192
1104	80-81	0.19013339658730644
1104	82-83	0.25590755876083904
1104	84-85	0.24729024510679665
1104	86-87	0.21663402178413804
1104	88-89	0.1909038146604658
1104	90-91	0.28606745881987194
1104	92-93	0.2488479045222718
1104	94-95	0.21146213593577556
1104	96-97	0.21340703741355327
1104	98-99	0.171858018226402
1104	100	0.2382051762010029
1105	1	-0.006877426067447345
1105	2	0.006532935600390033
1105	3	0.12198947308978703
1105	4	0.15616187630073597
1105	5	0.14234541759145714
1105	6	0.07397166984186754
1105	7	0.11202488215278095
1105	8	0.19717311363246637
1105	9	0.25068300159882995
1105	10-11	0.2288307558443421
1105	12-13	0.2589731570928677
1105	14-15	0.3093217716721455
1105	16-17	0.28303682412697384
1105	18-19	0.28939879754778985
1105	20-21	0.22677703724129827
1105	22-23	0.26543088517512814
1105	24-25	0.3047155702636246
1105	26-27	0.2960445978177475
1105	28-29	0.280781502276767
1105	30-31	0.32790566153026646
1105	32-33	0.25761654741258866
1105	34-35	0.24147464594502566
1105	36-37	0.25960650163138865
1105	38-39	0.3116924369084302
1105	40-41	0.3314123348990492
1105	42-43	0.3179599620764648
1105	44-45	0.25284072326241613
1105	46-47	0.22838962662789442
1105	48-49	0.23879601270390083
1105	50-51	0.1887321445183261
1105	52-53	0.22161232600265635
1105	54-55	0.2817402146692274
1105	56-57	0.22269834757927498
1105	58-59	0.3435454809667462
1105	60-61	0.20471112970979277
1105	62-63	0.1809063587819324
1105	64-65	0.20760641830766957
1105	66-67	0.19119998828671925
1105	68-69	0.24490711660693165
1105	70-71	0.17117556540836176
1105	72-73	0.13986178897401658
1105	74-75	0.17927024730749963
1105	76-77	0.1280769072616934
1105	78-79	0.2052926097272092
1105	80-81	0.1605803453175625
1105	82-83	0.22070812782022387
1105	84-85	0.19159745354879476
1105	86-87	0.14241463123742903
1105	88-89	0.15371773637346564
1105	90-91	0.18190970449472843
1105	92-93	0.16518389094771635
1105	94-95	0.08531073031014103
1105	96-97	0.12334407547747617
1105	98-99	0.11356366636418613
1105	100	0.18589300416982368
1106	1	0.09799583322931582
1106	2	0.08384358163019101
1106	3	0.08448066015121114
1106	4	0.13271559540024924
1106	5	0.09332040067534564
1106	6	0.14342472349551372
1106	7	-0.0041645845914857205
1106	8	0.0574077152403234
1106	9	0.12876713578577892
1106	10-11	0.13922300455926973
1106	12-13	0.12589644391280785
1106	14-15	0.20114446875469127
1106	16-17	0.15718190993779046
1106	18-19	0.10582374916535287
1106	20-21	0.1475976539183037
1106	22-23	0.2068517753489587
1106	24-25	0.216480508095934
1106	26-27	0.19192668819157888
1106	28-29	0.22848130559248148
1106	30-31	0.23224233707689734
1106	32-33	0.20504868975712043
1106	34-35	0.23297652840447824
1106	36-37	0.23456639980349792
1106	38-39	0.19432649215353592
1106	40-41	0.2102399893088318
1106	42-43	0.20425215574503142
1106	44-45	0.16041015029129113
1106	46-47	0.1504709469835248
1106	48-49	0.17873396330728042
1106	50-51	0.12105729345107363
1106	52-53	0.10035386203279018
1106	54-55	0.13799491687470322
1106	56-57	0.060651159123558784
1106	58-59	0.10864498239494935
1106	60-61	0.11875568098730582
1106	62-63	0.13114602339343406
1106	64-65	0.05967617734970787
1106	66-67	0.10565920113949545
1106	68-69	0.10723190276993932
1106	70-71	0.07804705501973785
1106	72-73	0.02190705317290309
1106	74-75	0.08186420861100885
1106	76-77	0.07505369473067702
1106	78-79	0.11523076537434918
1106	80-81	0.12784558204057817
1106	82-83	0.10763863848960398
1106	84-85	0.1141994273315774
1106	86-87	0.10070352527870341
1106	88-89	0.11106090549828451
1106	90-91	0.11933634220291367
1106	92-93	0.12206650023998122
1106	94-95	0.11389966646424199
1106	96-97	0.15897216394913016
1106	98-99	0.11915173975723548
1106	100	0.10597420149921177
1107	1	0.03533075381713502
1107	2	0.05714042712247647
1107	3	0.08319091953680413
1107	4	0.09066838138770805
1107	5	0.08052640500882546
1107	6	0.14315521395278097
1107	7	0.11033804762266897
1107	8	0.08381207127501966
1107	9	0.19496063207603953
1107	10-11	0.2057993123067945
1107	12-13	0.1547064102677922
1107	14-15	0.15097638497910282
1107	16-17	0.1560140138425794
1107	18-19	0.15301354695740343
1107	20-21	0.12313099185050902
1107	22-23	0.14246815510613686
1107	24-25	0.13825587715894017
1107	26-27	0.21106636183325378
1107	28-29	0.12744323139617109
1107	30-31	0.1487462767591694
1107	32-33	0.11877589754191575
1107	34-35	0.06994078987190022
1107	36-37	0.07434394010115142
1107	38-39	0.06506782709415404
1107	40-41	0.06080067321049398
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2216	42-43	-0.5211486030120724
2216	44-45	-0.4206811594155013
2216	46-47	-0.4427438047975656
2216	48-49	-0.462118736726822
2216	50-51	-0.39940628899591957
2216	52-53	-0.44132600448607207
2216	54-55	-0.3467460705241763
2216	56-57	-0.36800409036960957
2216	58-59	-0.3806399290685576
2216	60-61	-0.3177170889867611
2216	62-63	-0.36585820131993074
2216	64-65	-0.3512065563374449
2216	66-67	-0.40191828294370424
2216	68-69	-0.4310328854217502
2216	70-71	-0.40335655665825243
2216	72-73	-0.35595179173345315
2216	74-75	-0.3567885998396463
2216	76-77	-0.31966311010904036
2216	78-79	-0.30087318989831857
2216	80-81	-0.2904887307193107
2216	82-83	-0.2743818019093922
2216	84-85	-0.27940318265581254
2216	86-87	-0.22999898201689462
2216	88-89	-0.32927047106547747
2216	90-91	-0.31426834338165577
2216	92-93	-0.286524031365893
2216	94-95	-0.31218808982413293
2216	96-97	-0.3430412579152389
2216	98-99	-0.2884746776208189
2216	100	-0.33639578123821323
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	92.0
1	53.0
2	44.0
3	42.0
4	43.0
5	46.0
6	94.0
7	442.0
8	1441.0
9	1695.0
10	1790.0
11	2353.0
12	3245.0
13	3932.0
14	4700.0
15	5702.0
16	6358.0
17	6807.0
18	7680.0
19	8736.0
20	10079.0
21	11230.0
22	12975.0
23	15006.0
24	17950.0
25	22754.0
26	26000.0
27	24477.0
28	26465.0
29	34285.0
30	38772.0
31	42583.0
32	54930.0
33	73906.0
34	108207.0
35	169716.0
36	321251.0
37	747149.0
38	1095091.0
39	371767.0
40	932.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.03866688869736	19.554011100314565	13.38707817981192	24.02024383117616
2	15.024042198676902	20.826817522546992	40.376017914440624	23.773122364335485
3	18.050856370306807	26.01817148135221	30.501049409906976	25.42992273843401
4	12.338906811417974	17.604573968968538	39.440880091523276	30.615639128090216
5	13.100531061827962	38.28543940709832	35.6204973221327	12.99353220894102
6	29.93546499044321	38.41869411868711	18.603763576891772	13.042077313977913
7	26.432641087759894	33.07352279557805	23.09447548772771	17.399360628934346
8	25.126545661600773	35.57519674550712	21.18566106429428	18.112596528597827
9	25.953926923087472	16.060649197959744	20.62073949931277	37.36468437964
10-11	24.056475877103072	26.537785240535495	29.406311950622243	19.999426931739187
12-13	24.858898278735797	24.451550503381686	28.660155483184234	22.02939573469828
14-15	22.30435834952516	25.867652178260258	26.026669414247138	25.801320057967448
16-17	22.225949664591155	28.647780778021716	26.70621684549245	22.420052711894684
18-19	22.158062897103946	28.335962720868174	27.8135805643477	21.692393817680184
20-21	23.21953385993729	27.497923004785278	27.420086170724968	21.86245696455246
22-23	23.046545305798478	27.322075910336057	27.109745700587577	22.521633083277884
24-25	22.61743228593632	27.50829589581577	27.40886554758247	22.46540627066544
26-27	22.64733347365129	27.73700411611194	27.063737018747386	22.551925391489387
28-29	22.52114698181131	27.817738164548665	27.155452796888387	22.50566205675164
30-31	22.826062603080906	27.642975058478243	27.03486533352375	22.496097004917097
32-33	22.648699243138644	27.78258994825566	26.868128526663448	22.70058228194225
34-35	22.971542076594517	27.70265069444942	26.94277489254755	22.38303233640851
36-37	23.024556014406553	27.453558866084542	26.975169609904842	22.546715509604066
38-39	22.782165161589678	27.565765917098034	27.094739290626368	22.557329630685917
40-41	22.858469442832487	27.438108037948627	27.196504816897534	22.506917702321356
42-43	22.74947709053647	27.384205718017462	27.124827989806597	22.741489201639467
44-45	22.760764506455573	27.586897110729286	26.985846724605445	22.666491658209704
46-47	22.85182452374564	27.52053503914921	26.816672561406936	22.81096787569822
48-49	22.799301585549596	27.44116904078084	27.05288829256327	22.706641081106298
50-51	22.74639795505655	27.543941144607913	26.947057192282404	22.76260370805313
52-53	22.810509997168996	27.566233255029683	26.914858083084596	22.70839866471672
54-55	22.696585359904013	27.57427455859103	26.929613926279632	22.799526155225326
56-57	22.743237690702724	27.614814227549623	26.94891648301218	22.693031598735473
58-59	22.783883789111634	27.423385184880267	27.065943397808546	22.726787628199556
60-61	22.745677509572246	27.489889566593778	27.013083875051723	22.751349048782252
62-63	22.738274371698417	27.42523268311647	26.99058595745005	22.845906987735066
64-65	22.62258172318155	27.414694073194113	27.00809060107184	22.954633602552498
66-67	22.74713897180601	27.515404950044157	26.84301015399538	22.894445924154454
68-69	22.71749353818823	27.450072184151075	26.959884700512387	22.87254957714831
70-71	22.71534100706657	27.259174579438568	26.940463134810035	23.08502127868483
72-73	22.801309188837134	27.37362132072687	26.922146928211717	22.90292256222428
74-75	22.7448190041834	27.590513154482398	26.865511443098427	22.799156398235773
76-77	22.85218000091782	27.364110229423304	26.841516198097548	22.942193571561322
78-79	22.809513601989536	27.477781297900904	26.79665271864462	22.916052381464937
80-81	22.79779995737013	27.531590469084044	26.823333966580993	22.84727560696483
82-83	22.951745941043264	27.359695734708865	26.99823891186067	22.6903194123872
84-85	22.962605311249902	27.500458512996996	26.788257859476854	22.748678316276244
86-87	22.81506447920548	27.545475722803143	26.890804601205854	22.74865519678552
88-89	22.90370391148835	27.346970759042843	26.83118433758193	22.91814099188688
90-91	22.94772760292901	27.376199261176904	26.802956572990222	22.87311656290386
92-93	23.005628259819268	27.334313022842196	26.834375606493516	22.82568311084502
94-95	22.76070142934746	27.335692735196044	27.04781379254512	22.855792042911375
96-97	22.879003602664973	27.274739679213262	27.003781342278177	22.84247537584359
98-99	22.804224479920823	27.418537012526293	26.80472329979715	22.972515207755734
100	22.912488981888373	27.275005663408464	26.77848557256221	23.03401978214095
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	979.0
1	746.5
2	575.5
3	645.5
4	587.0
5	485.0
6	449.0
7	441.0
8	444.0
9	462.5
10	424.0
11	378.5
12	383.5
13	413.5
14	461.0
15	517.5
16	595.5
17	755.5
18	934.0
19	1160.5
20	1622.5
21	2419.0
22	3687.0
23	5556.0
24	7866.0
25	10909.0
26	15130.5
27	19723.5
28	24757.5
29	30577.5
30	37144.5
31	44624.0
32	52899.0
33	59988.5
34	65340.0
35	71483.5
36	79027.0
37	87520.0
38	94004.0
39	97978.0
40	101636.5
41	105340.0
42	110054.0
43	116393.0
44	121979.0
45	127938.5
46	133314.5
47	134386.0
48	134583.5
49	133397.0
50	128662.5
51	125388.5
52	121822.0
53	116493.5
54	111112.5
55	102216.5
56	92520.0
57	82372.0
58	72551.5
59	63787.0
60	52388.5
61	41797.5
62	33501.0
63	25357.5
64	18230.0
65	12993.0
66	9426.5
67	6946.0
68	5142.5
69	3785.5
70	2916.0
71	2172.5
72	1608.0
73	1327.0
74	1020.0
75	750.0
76	561.5
77	381.5
78	259.0
79	173.0
80	118.5
81	87.0
82	64.0
83	46.5
84	41.0
85	30.5
86	19.5
87	17.0
88	15.5
89	13.0
90	10.0
91	9.0
92	8.0
93	4.5
94	6.0
95	7.0
96	6.0
97	5.0
98	6.5
99	8.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06077748855469059
2	0.010302302473162198
3	0.012344474856895531
4	0.0104547033973214
5	0.012527355965886576
6	0.013106479477691552
7	0.0029260977438567187
8	0.005669314378722392
9	0.0075895660231283645
10-11	0.007117123158234831
12-13	0.00633987844502289
14-15	0.010911906169799014
16-17	0.010546143951816925
18-19	0.008275370181844783
20-21	0.0073000042672258765
22-23	0.002468894971379106
24-25	0.004008144305387068
26-27	0.005349272437988064
28-29	0.006400838814686572
30-31	0.009951780347596027
32-33	0.011110027371205979
34-35	0.01266451679762986
36-37	0.01969019940136917
38-39	0.019644479124121407
40-41	0.01976639986344877
42-43	0.02604531793880798
44-45	0.029824860857956242
46-47	0.03230899592175127
48-49	0.03369584433160003
50-51	0.03415304710407764
52-53	0.032507117123158236
54-55	0.038557433812278635
56-57	0.0407672472125871
58-59	0.038557433812278635
60-61	0.039227997878579134
62-63	0.03489981163245774
64-65	0.03694198401619107
66-67	0.038816515383349286
68-69	0.041468291463719435
70-71	0.041590212203046796
72-73	0.03755158771282789
74-75	0.03994428222212739
76-77	0.04006620296145476
78-79	0.038496473442614955
80-81	0.04355618412470053
82-83	0.040035722776622915
84-85	0.03646954115129754
86-87	0.03529605403527167
88-89	0.03264427795490152
90-91	0.03488457154004182
92-93	0.03686578355411147
94-95	0.04012716333111844
96-97	0.03549417523667863
98-99	0.03288811943355625
100	0.030266823538017934
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3280820.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.722547828271786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.34551624825372	43.930040705456
2	15.083004081612048	18.317568734794122
3	5.750512947289041	10.475573924365648
4	2.6818547783091122	6.513962201774172
5	1.4349416236900054	4.356665568764717
6	0.8158207685050859	2.9723229380908514
7	0.5047007418195846	2.1452700453872784
8	0.33404292389004675	1.6227149938087295
9	0.23256920893881092	1.2709975421853255
>10	0.7978703673814835	7.4889815860517
>50	0.016316012884889965	0.6687845389504437
>100	0.0028502974261768468	0.23711722037093136
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.144055449552246E-5	0.0
3	0.0	0.0	0.0	9.144055449552246E-5	0.0
4	0.0	0.0	0.0	1.2192073932736329E-4	3.048018483184082E-5
5	0.0	0.0	0.0	1.524009241592041E-4	3.048018483184082E-5
6	0.0	0.0	0.0	1.524009241592041E-4	3.048018483184082E-5
7	0.0	0.0	0.0	1.524009241592041E-4	3.048018483184082E-5
8	0.0	0.0	0.0	1.524009241592041E-4	6.096036966368164E-5
9	0.0	0.0	0.0	2.1336129382288575E-4	9.144055449552246E-5
10-11	0.0	0.0	0.0	3.3528203315024905E-4	1.2192073932736329E-4
12-13	0.0	0.0	0.0	3.810023103980102E-4	1.2192073932736329E-4
14-15	0.0	0.0	0.0	4.572027724776123E-4	2.1336129382288575E-4
16-17	0.0	0.0	0.0	4.572027724776123E-4	3.6576221798208984E-4
18-19	0.0	0.0	0.0	4.572027724776123E-4	4.572027724776123E-4
20-21	0.0	0.0	0.0	5.486433269731348E-4	4.572027724776123E-4
22-23	0.0	0.0	0.0	7.924848056278613E-4	5.791235118049755E-4
24-25	0.0	0.0	0.0	0.0011887272084417921	6.400838814686572E-4
26-27	0.0	0.0	0.0	0.0021488530306447776	6.400838814686572E-4
28-29	0.0	0.0	0.0	0.006248437890527368	6.400838814686572E-4
30-31	0.0	0.0	0.0	0.014721929273779116	6.705640663004981E-4
32-33	0.0	0.0	0.0	0.03351296322260898	7.620046207960205E-4
34-35	0.0	0.0	0.0	0.06233197798111448	7.77244713211941E-4
36-37	0.0	0.0	0.0	0.10916478197523789	7.924848056278613E-4
38-39	0.0	0.0	0.0	0.170231832285831	8.077248980437817E-4
40-41	0.0	0.0	0.0	0.2362519126315982	8.229649904597021E-4
42-43	0.0	0.0	0.0	0.30054986253436644	8.382050828756226E-4
44-45	0.0	0.0	0.0	0.3804536670710371	8.686852677074634E-4
46-47	0.0	0.0	0.0	0.47671009076999044	9.448857297870654E-4
48-49	0.0	0.0	0.0	0.5769746587743309	0.0010972866539462696
50-51	0.0	0.0	0.0	0.6784736742643608	0.0010972866539462696
52-53	0.0	0.0	0.0	0.7729622472430673	0.0012039673008577125
54-55	0.0	0.0	0.0	0.874887985320743	0.0013258880401850756
56-57	0.0	0.0	0.0	0.9958638389183192	0.0013411281326009962
58-59	0.0	0.0	0.0	1.1268219530483234	0.001447808779512439
60-61	0.0	0.0	0.0	1.2637389433129522	0.0015849696112557226
62-63	0.0	0.0	0.0	1.3986442413786797	0.0016459299809194043
64-65	0.0	0.0	0.0	1.5370852408849007	0.0016459299809194043
66-67	0.0	0.0	0.0	1.6921074609396431	0.0016764101657512451
68-69	0.0	0.0	0.0	1.8649910693058442	0.0017221304429990065
70-71	0.0	0.0	0.0	2.04159326022153	0.0018288110899104492
72-73	0.0	0.0	0.0	2.221974994056364	0.002164093123060698
74-75	0.0	0.0	0.0	2.3999487932894823	0.0021793332154766186
76-77	0.0	0.0	0.0	2.5893679019269573	0.0022098134003084597
78-79	0.0	0.0	0.0	2.7978218859919166	0.00222505349272438
80-81	0.0	0.0	0.0	3.0234057339323703	0.002301253954803982
82-83	0.0	0.0	0.0	3.255725702720661	0.0024688949713791066
84-85	0.0	0.0	0.0	3.486171140141794	0.0024688949713791066
86-87	0.0	0.0	0.0	3.722438292865808	0.0024841350637950267
88	0.0	0.0	0.0	3.9175876762516686	0.002529855341042788
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5480	0.0	40.58423	1
GGTATCA	3195	0.0	39.734673	1
ATCAACG	6340	0.0	34.468987	3
CAACGCA	6330	0.0	34.22542	5
TCAACGC	6660	0.0	32.671192	4
TATCAAC	6830	0.0	32.340145	2
AACGCAG	6955	0.0	31.485258	6
ACGCAGA	7760	0.0	27.793482	7
CGCAGAG	7975	0.0	26.929226	8
TACATGG	10175	0.0	23.555948	2
GTACATG	10675	0.0	23.036184	1
ACATGGG	9930	0.0	22.62265	3
GTACTAG	970	0.0	22.297842	1
GCAGAGT	9445	0.0	22.140959	9
CATGGGG	7170	0.0	20.38447	4
ATTGCGT	455	0.0	19.622787	6
AGAGTAC	7780	0.0	18.634192	10-11
CTAGTAC	1020	0.0	17.50847	3
TAGTACT	1275	0.0	17.323908	4
CAGAGTA	8805	0.0	16.945305	10-11
>>END_MODULE