FastQCFastQC Report
Mon 16 Jan 2017
ERR1146491_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146491_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3280820
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100750.30708786218079626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85460.26048365957291164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47620.145146640169226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58900.059.6164741
GTATCAA110000.043.4754181
ATCAACG139400.033.931853
CAACGCA140550.033.6542135
TCAACGC141800.033.3907054
TATCAAC143750.033.068652
AACGCAG149250.031.805386
ACGCAGA165250.028.5530647
CGCAGAG168050.027.9934238
GCAGAGT188900.024.7045969
TACATGG95850.024.0606252
GTACATG97550.023.9331
ACATGGG95900.022.6024063
GAGTACT132750.022.03765112-13
AGAGTAC168000.021.9594210-11
CAGAGTA181800.021.4816510-11
CATGGGG71100.021.4263274
GTACTAG8600.020.7983021
AGTACTT137750.020.43599112-13
GTACTTT146100.019.8309414-15
Produced by FastQC (version 0.11.5)