##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146491_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3280820
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.602818197889555	34.0	31.0	34.0	31.0	34.0
2	32.66465334885791	34.0	31.0	34.0	31.0	34.0
3	32.76562901957438	34.0	31.0	34.0	31.0	34.0
4	36.23351692564664	37.0	37.0	37.0	35.0	37.0
5	36.247151626727465	37.0	37.0	37.0	35.0	37.0
6	36.31894069165635	37.0	37.0	37.0	35.0	37.0
7	36.30854786303424	37.0	37.0	37.0	35.0	37.0
8	36.31731396419188	37.0	37.0	37.0	35.0	37.0
9	38.14135002834657	39.0	39.0	39.0	37.0	39.0
10-11	38.10646027517511	39.0	38.5	39.0	37.0	39.0
12-13	38.08692095268866	39.0	38.5	39.0	37.0	39.0
14-15	39.57415569888016	41.0	39.5	41.0	37.0	41.0
16-17	39.568899238604985	41.0	39.0	41.0	37.0	41.0
18-19	39.61705046299401	41.0	40.0	41.0	37.0	41.0
20-21	39.647907230509446	41.0	40.0	41.0	37.0	41.0
22-23	39.60906511177083	41.0	40.0	41.0	37.0	41.0
24-25	39.603629123207	41.0	40.0	41.0	37.0	41.0
26-27	39.5376107192714	41.0	40.0	41.0	37.0	41.0
28-29	39.45308307069574	41.0	39.0	41.0	36.0	41.0
30-31	39.358597850537365	41.0	39.0	41.0	36.0	41.0
32-33	39.24739424899873	41.0	39.0	41.0	35.0	41.0
34-35	39.17277647051652	41.0	39.0	41.0	35.0	41.0
36-37	39.09141799915875	41.0	39.0	41.0	35.0	41.0
38-39	38.99992303753329	41.0	39.0	41.0	35.0	41.0
40-41	38.91860632402875	41.0	39.0	41.0	35.0	41.0
42-43	38.83188440694704	41.0	39.0	41.0	35.0	41.0
44-45	38.70395861400503	41.0	39.0	41.0	35.0	41.0
46-47	38.49700958906615	40.5	38.0	41.0	35.0	41.0
48-49	38.313199748843275	40.0	38.0	41.0	34.0	41.0
50-51	38.186537664364394	40.0	38.0	41.0	34.0	41.0
52-53	38.06877487945087	40.0	38.0	41.0	34.0	41.0
54-55	37.945154869819135	40.0	37.0	41.0	34.0	41.0
56-57	37.77463454258387	40.0	37.0	41.0	33.5	41.0
58-59	37.59229582848191	40.0	36.5	41.0	33.0	41.0
60-61	37.42416865295871	39.5	36.0	41.0	33.0	41.0
62-63	37.18283264549716	39.0	35.5	41.0	33.0	41.0
64-65	36.89802640803214	39.0	35.0	41.0	33.0	41.0
66-67	36.64355587932285	38.5	35.0	41.0	32.0	41.0
68-69	36.320851037240686	37.0	35.0	40.0	32.0	41.0
70-71	36.021679183862574	37.0	35.0	39.5	32.0	41.0
72-73	35.680791692320824	36.5	35.0	39.0	32.0	41.0
74-75	35.34013493577825	36.0	35.0	39.0	31.5	41.0
76-77	35.01693555269719	35.5	35.0	38.0	31.0	40.0
78-79	34.70520952079053	35.0	35.0	37.0	31.0	39.0
80-81	34.416123408172346	35.0	35.0	37.0	31.0	39.0
82-83	34.1485736797508	35.0	35.0	36.0	31.0	38.0
84-85	33.92741570704885	35.0	34.5	36.0	31.0	37.0
86-87	33.732225785017164	35.0	34.0	36.0	31.0	37.0
88-89	33.568282014862135	35.0	34.0	35.0	30.5	36.5
90-91	33.47508625892307	35.0	34.0	35.0	30.5	36.0
92-93	33.38707213440543	35.0	34.0	35.0	30.0	36.0
94-95	33.308040825159566	35.0	34.0	35.0	30.0	36.0
96-97	33.26046460945739	35.0	34.0	35.0	30.0	36.0
98-99	33.206267030803275	35.0	34.0	35.0	30.0	35.0
100	32.67316615967959	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2213350849670448
1101	2	0.22927893509952213
1101	3	0.20146836914813093
1101	4	0.14190900230965298
1101	5	0.07610147160280434
1101	6	0.07356375211837474
1101	7	0.14120251312768062
1101	8	0.14913033008444643
1101	9	0.13295918968034925
1101	10-11	0.15200113651361846
1101	12-13	0.14718459170655507
1101	14-15	0.2353805743204802
1101	16-17	0.2421438307886845
1101	18-19	0.2258596988734638
1101	20-21	0.2536183288065317
1101	22-23	0.26508349403275844
1101	24-25	0.2729207872283155
1101	26-27	0.32079272042077633
1101	28-29	0.30272971407968186
1101	30-31	0.3035404821013259
1101	32-33	0.29371243790252777
1101	34-35	0.29812032804145616
1101	36-37	0.32323876594981016
1101	38-39	0.31663348342068076
1101	40-41	0.30475256184186605
1101	42-43	0.2950303586644978
1101	44-45	0.3174003580762843
1101	46-47	0.3556751333438726
1101	48-49	0.29234619346289037
1101	50-51	0.3204698517063562
1101	52-53	0.32081247927318657
1101	54-55	0.23696852055740436
1101	56-57	0.28551380983689256
1101	58-59	0.2934034746456504
1101	60-61	0.1542432259071731
1101	62-63	0.2071468092869324
1101	64-65	0.21307465343159038
1101	66-67	0.19109186394221211
1101	68-69	0.18493262886769912
1101	70-71	0.10828865540288035
1101	72-73	0.11200392166451678
1101	74-75	0.12710720844130918
1101	76-77	0.18119427264684873
1101	78-79	0.15798983684435086
1101	80-81	0.16780733100149092
1101	82-83	0.22046765317738704
1101	84-85	0.18770780502040907
1101	86-87	0.13105455316917158
1101	88-89	0.19142569645655527
1101	90-91	0.16210378677962467
1101	92-93	0.11394854065537885
1101	94-95	0.0860363749116857
1101	96-97	0.1511640145474047
1101	98-99	0.10273499704172906
1101	100	0.13553830740581674
1102	1	0.22148851004185843
1102	2	0.2342418123108274
1102	3	0.21997136917628524
1102	4	0.14529491292827146
1102	5	0.07220153098902671
1102	6	0.10250365684444773
1102	7	0.10806413682892924
1102	8	0.12618312238880947
1102	9	0.16613228260186474
1102	10-11	0.15179435662916063
1102	12-13	0.17658933121349207
1102	14-15	0.26298956851751853
1102	16-17	0.22967720354218812
1102	18-19	0.2413738696549288
1102	20-21	0.26472452839543337
1102	22-23	0.25824815897416187
1102	24-25	0.2464872786800214
1102	26-27	0.24975139132126856
1102	28-29	0.27206421721921714
1102	30-31	0.2238695924854639
1102	32-33	0.24971119834311395
1102	34-35	0.28335984394135494
1102	36-37	0.2551194725946644
1102	38-39	0.22737024694832542
1102	40-41	0.2631921248411757
1102	42-43	0.25470236177203986
1102	44-45	0.2312856153184626
1102	46-47	0.2928755624861239
1102	48-49	0.3269878635212322
1102	50-51	0.26498932885161963
1102	52-53	0.2962819056073869
1102	54-55	0.2804340522304827
1102	56-57	0.27197472691887725
1102	58-59	0.19873148433687504
1102	60-61	0.23260560281514842
1102	62-63	0.23241467122932846
1102	64-65	0.28357107553965477
1102	66-67	0.19729710029682224
1102	68-69	0.2015670106750349
1102	70-71	0.21551550437501277
1102	72-73	0.20416191653477256
1102	74-75	0.21985802564181256
1102	76-77	0.16770278509876846
1102	78-79	0.16395405664503926
1102	80-81	0.19430931288869857
1102	82-83	0.17929069887150462
1102	84-85	0.17365973161698633
1102	86-87	0.1927726292347316
1102	88-89	0.20688067299643365
1102	90-91	0.1467799974778501
1102	92-93	0.14491056866929597
1102	94-95	0.22216961187039885
1102	96-97	0.20993725708636646
1102	98-99	0.19370526745382932
1102	100	0.26008795121646244
1103	1	0.18255623210203709
1103	2	0.17912701504778994
1103	3	0.21218800932006587
1103	4	0.14165659145075438
1103	5	0.09200532434809361
1103	6	0.09328297802577623
1103	7	0.1386027983349294
1103	8	0.12353313418606149
1103	9	0.10302620944554519
1103	10-11	0.14373473409089144
1103	12-13	0.13893848750109328
1103	14-15	0.23441565081044047
1103	16-17	0.21122831574655976
1103	18-19	0.20017856997972672
1103	20-21	0.21335948849396402
1103	22-23	0.1993371160807058
1103	24-25	0.23055979117589942
1103	26-27	0.17902715115607037
1103	28-29	0.22090243929864073
1103	30-31	0.2521769739649571
1103	32-33	0.2867708200446941
1103	34-35	0.2958429638365345
1103	36-37	0.24832988446085125
1103	38-39	0.29201294302946224
1103	40-41	0.27597059427793624
1103	42-43	0.30434822470307665
1103	44-45	0.2573952364343626
1103	46-47	0.3280027573180959
1103	48-49	0.3559498458437602
1103	50-51	0.38289163174135865
1103	52-53	0.3944070844486447
1103	54-55	0.36127302063226097
1103	56-57	0.37047866226325965
1103	58-59	0.3566368641329518
1103	60-61	0.2769610066240702
1103	62-63	0.3186481090645188
1103	64-65	0.32764420299845654
1103	66-67	0.31044280091111176
1103	68-69	0.3492420952219817
1103	70-71	0.26892116487866247
1103	72-73	0.3221270230363089
1103	74-75	0.2731298166648557
1103	76-77	0.27161876024589304
1103	78-79	0.23135483007597202
1103	80-81	0.2835516764715109
1103	82-83	0.2914569036884629
1103	84-85	0.2872562481657681
1103	86-87	0.29683706354414596
1103	88-89	0.20622126121893558
1103	90-91	0.1858683170309945
1103	92-93	0.20807243820679133
1103	94-95	0.19373714609496773
1103	96-97	0.189648343738412
1103	98-99	0.20850762380768373
1103	100	0.24238402616329324
1104	1	0.18936810611488397
1104	2	0.16032530910600684
1104	3	0.17127508370992928
1104	4	0.11784365372518124
1104	5	0.09244603709119303
1104	6	0.08285481420290353
1104	7	0.10876826846372012
1104	8	0.08782762144207368
1104	9	0.12274379760754073
1104	10-11	0.14303281707383064
1104	12-13	0.11412936113491412
1104	14-15	0.18512445917431108
1104	16-17	0.20080765459964312
1104	18-19	0.21517364044375142
1104	20-21	0.1937324881971847
1104	22-23	0.20032691355291377
1104	24-25	0.16938547233552015
1104	26-27	0.17125104434465044
1104	28-29	0.1818098851765626
1104	30-31	0.23573083039597265
1104	32-33	0.2231970342833236
1104	34-35	0.20413219810786387
1104	36-37	0.2807797915943979
1104	38-39	0.28108348930208393
1104	40-41	0.27249213140395057
1104	42-43	0.23986022398282358
1104	44-45	0.22690852950888996
1104	46-47	0.23134911751989762
1104	48-49	0.2135001498966531
1104	50-51	0.25950317874868034
1104	52-53	0.2504310107601455
1104	54-55	0.16694565498296043
1104	56-57	0.18427919556458505
1104	58-59	0.20574082232248259
1104	60-61	0.2589882074368006
1104	62-63	0.2423923000481949
1104	64-65	0.1775476129924698
1104	66-67	0.17234326763313135
1104	68-69	0.24164205120628424
1104	70-71	0.20784966048654496
1104	72-73	0.1550696614662357
1104	74-75	0.18692851597872817
1104	76-77	0.15753807146688814
1104	78-79	0.1334371283178939
1104	80-81	0.15696291536894336
1104	82-83	0.14288808080829796
1104	84-85	0.16647789277456582
1104	86-87	0.12046332357689238
1104	88-89	0.10465336867058994
1104	90-91	0.13638816911156937
1104	92-93	0.15523942776640354
1104	94-95	0.09027329954525953
1104	96-97	0.09363104201190708
1104	98-99	0.13361063276977347
1104	100	0.16951748154015434
1105	1	0.14943991327521644
1105	2	0.18446799590167018
1105	3	0.18470428663476923
1105	4	0.1466375552483541
1105	5	0.047350598713279624
1105	6	0.056882736411495216
1105	7	0.0978686618778184
1105	8	0.10533085435609024
1105	9	0.10510333661051874
1105	10-11	0.12549354278135638
1105	12-13	0.12956982503045822
1105	14-15	0.19742077014797133
1105	16-17	0.16763060352565873
1105	18-19	0.1172961616486532
1105	20-21	0.14655152032580787
1105	22-23	0.15421162533235844
1105	24-25	0.12327213229758627
1105	26-27	0.1466683058603664
1105	28-29	0.17059144187426512
1105	30-31	0.16517632773017255
1105	32-33	0.1238765223674605
1105	34-35	0.15400142040999754
1105	36-37	0.09198257816078126
1105	38-39	0.11918856334370531
1105	40-41	0.1069078702486479
1105	42-43	0.13762033665430096
1105	44-45	0.2055793510313677
1105	46-47	0.16876876389624584
1105	48-49	0.1854373870553374
1105	50-51	0.1384714544953951
1105	52-53	0.11160765129027794
1105	54-55	0.06824354849862146
1105	56-57	0.11117592359187967
1105	58-59	0.13577550234893465
1105	60-61	0.17116508531260166
1105	62-63	0.09325478662771758
1105	64-65	0.16686361320248722
1105	66-67	0.12797355103906227
1105	68-69	0.1585525376793555
1105	70-71	0.12395728189574129
1105	72-73	0.09101486621667476
1105	74-75	0.06394523542450514
1105	76-77	0.06888362180591656
1105	78-79	0.06900514590665807
1105	80-81	0.0912541130759763
1105	82-83	0.09516107536217788
1105	84-85	0.1210911535784831
1105	86-87	0.11362128246120307
1105	88-89	0.1065897671604219
1105	90-91	0.09901257066906766
1105	92-93	0.08471733145538707
1105	94-95	0.08335124039224695
1105	96-97	0.0920052360998369
1105	98-99	0.1043594785309665
1105	100	0.12198410643969027
1106	1	0.09080339119594072
1106	2	0.07800643608646851
1106	3	0.10836775619602435
1106	4	0.04927015941452595
1106	5	0.04941365440159018
1106	6	0.06929860932221743
1106	7	0.0437386862149296
1106	8	0.02032917457451333
1106	9	0.04072685102903506
1106	10-11	0.050237277511506306
1106	12-13	0.04440300615823389
1106	14-15	0.10764977248389584
1106	16-17	0.08567839764910445
1106	18-19	0.07476267248205914
1106	20-21	0.07013502282024575
1106	22-23	0.09690757224810653
1106	24-25	0.10817207882847413
1106	26-27	0.09526935536896985
1106	28-29	0.11143868269500956
1106	30-31	0.13404279850118428
1106	32-33	0.14826505821304892
1106	34-35	0.12288760692727152
1106	36-37	0.15670953084772066
1106	38-39	0.09866841463966125
1106	40-41	0.1351181063486564
1106	42-43	0.05312874265987233
1106	44-45	-0.017343279648542875
1106	46-47	0.012153026945306067
1106	48-49	0.06392782334113889
1106	50-51	0.05154977648034986
1106	52-53	0.10277909784247186
1106	54-55	0.047327034431837944
1106	56-57	0.06809055125606989
1106	58-59	0.027808290423593007
1106	60-61	0.01416623198040412
1106	62-63	-0.05452750737034506
1106	64-65	-0.00690703115542135
1106	66-67	-0.024658027908309066
1106	68-69	0.012015647940856411
1106	70-71	-0.01415341334456599
1106	72-73	-0.09624623808593213
1106	74-75	-0.04015169664764784
1106	76-77	-0.09315915032264854
1106	78-79	-0.07086545570750502
1106	80-81	-0.0934497800741454
1106	82-83	-0.03438888512746274
1106	84-85	-0.05695922725086433
1106	86-87	-0.04475530721941112
1106	88-89	-0.0014657713081547286
1106	90-91	0.010065848924490695
1106	92-93	-0.0770415339270798
1106	94-95	-0.05946363190491866
1106	96-97	-0.01882549081184237
1106	98-99	-0.09460852044697532
1106	100	-0.06849618491172293
1107	1	0.09014400488381824
1107	2	0.038602676492573096
1107	3	0.10116923081658769
1107	4	0.033107294463860626
1107	5	0.04317999373876802
1107	6	0.0067804884023559
1107	7	0.010611107616867343
1107	8	0.05793500492146819
1107	9	-0.0055522280293658355
1107	10-11	0.06986233809656284
1107	12-13	0.0645135179167724
1107	14-15	0.07877236451312086
1107	16-17	0.07064553302858201
1107	18-19	0.04121149630670118
1107	20-21	0.03603024732439053
1107	22-23	0.049275678607003215
1107	24-25	0.06041018344416926
1107	26-27	0.07089011048126537
1107	28-29	0.16836537982472777
1107	30-31	0.14501021829158844
1107	32-33	0.15225599611525098
1107	34-35	0.14068243051990947
1107	36-37	0.1462630172516839
1107	38-39	0.15103914158705578
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2216	32-33	-0.340539912742976
2216	34-35	-0.385512608688785
2216	36-37	-0.37323482786865725
2216	38-39	-0.34177993750120805
2216	40-41	-0.28868120152765897
2216	42-43	-0.3895836838471709
2216	44-45	-0.393763344799666
2216	46-47	-0.37835183031918973
2216	48-49	-0.31783748330337147
2216	50-51	-0.3712528743443144
2216	52-53	-0.3458154440711496
2216	54-55	-0.43782730305199635
2216	56-57	-0.39562416710580095
2216	58-59	-0.4352073194496029
2216	60-61	-0.42392574191085686
2216	62-63	-0.37949244032799356
2216	64-65	-0.34743160097086445
2216	66-67	-0.3405405530766572
2216	68-69	-0.3598392278654501
2216	70-71	-0.3069153039516692
2216	72-73	-0.3858792056332945
2216	74-75	-0.3549929175975066
2216	76-77	-0.33648017393600327
2216	78-79	-0.3285706554918377
2216	80-81	-0.25565822870051846
2216	82-83	-0.30512994971633844
2216	84-85	-0.33665935769665367
2216	86-87	-0.32798514345922314
2216	88-89	-0.2832917517988065
2216	90-91	-0.2749981921448139
2216	92-93	-0.33400693015696703
2216	94-95	-0.2551713458869642
2216	96-97	-0.238338065454883
2216	98-99	-0.24734290641497836
2216	100	-0.45042557816235274
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	15.0
8	70.0
9	172.0
10	232.0
11	208.0
12	205.0
13	187.0
14	285.0
15	519.0
16	991.0
17	1788.0
18	3241.0
19	5335.0
20	7886.0
21	11329.0
22	16478.0
23	20779.0
24	21026.0
25	18524.0
26	16410.0
27	16934.0
28	19635.0
29	23811.0
30	29715.0
31	37736.0
32	49642.0
33	65705.0
34	94663.0
35	151723.0
36	282286.0
37	711181.0
38	1196700.0
39	473668.0
40	1741.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.00278179294267	19.372173169811298	13.72070750084283	23.904337536403204
2	15.161670215946742	20.872238121098587	40.03615384146006	23.929937821494605
3	17.8083427695337	26.151660949065853	30.92820207235724	25.111794209043207
4	12.178266409007504	17.77595235337507	39.0286574697789	31.017123767838527
5	12.709514923097565	38.01837486430776	35.625038115798844	13.647072096795835
6	29.66954618611003	38.490325058525166	18.16712592664846	13.67300282871635
7	25.66478065385503	33.56430208432711	23.06521633774981	17.705700924068047
8	25.338573893112088	34.92873732786316	20.98286404008754	18.749824738937217
9	26.04394633049055	15.107869374119884	20.699032558933435	38.149151736456126
10-11	24.010628440450862	26.21942075456746	29.24118055851891	20.528770246462773
12-13	25.229805353539664	24.198035856889437	28.908336940155205	21.663821849415697
14-15	22.394508080296998	25.21592162934876	27.021552538694593	25.368017751659643
16-17	21.584954371163306	28.16449850951896	27.54005096286904	22.71049615644869
18-19	21.434153656707775	27.059408928255742	29.96758432343134	21.538853091605148
20-21	22.409397650587355	26.20162032662566	29.921848806091162	21.467133216695828
22-23	22.230140339139588	26.115333062566247	29.60204096406291	22.05248563423126
24-25	21.851503587517755	26.305847318658138	29.908010802177504	21.9346382916466
26-27	21.885763315268743	26.549109673801063	29.5691321072171	21.995994903713097
28-29	21.77737909689942	26.562265921669876	29.678880535792963	21.981474445637744
30-31	22.082951314551014	26.358655895362617	29.598840654276255	21.959552135810114
32-33	21.95988459379604	26.541293258466524	29.347442261464153	22.151379886273283
34-35	22.253559678295424	26.561628444994994	29.32143451514795	21.863377361561632
36-37	22.362242548944334	26.289417483910523	29.28884429940501	22.059495667740137
38-39	22.158062627817742	26.451778677390493	29.316069628865815	22.074089065925953
40-41	22.25379850083883	26.36181528227537	29.286526813233976	22.097859403651828
42-43	22.257931729646586	26.350416034896423	29.06830952075785	22.323342714699137
44-45	22.31479786716687	26.58905959079194	28.82578504849655	22.27035749354464
46-47	22.550655981897137	26.62346489750845	28.343346621730568	22.482532498863847
48-49	22.61131683711834	26.732911829548527	28.16322151382303	22.4925498195101
50-51	22.547846261020005	26.94897531680207	27.969353155157993	22.533825267019925
52-53	22.55899994086747	27.046061803859224	27.900222082282006	22.494716172991307
54-55	22.40945302618791	27.074364718399146	27.837828899591997	22.678353355820942
56-57	22.524950937861433	27.061771888068982	27.818937429218614	22.594339744850974
58-59	22.396732920752203	26.901113578889568	28.089965384212682	22.612188116145546
60-61	22.439157316179564	26.989373790675074	28.019517452684685	22.551951440460673
62-63	22.39386829115741	26.897352521441537	28.030878004085615	22.67790118331544
64-65	22.287581769075793	26.82364032930604	28.04909627082262	22.839681630795543
66-67	22.348814316145884	26.940726399676652	27.88346367552012	22.826995608657345
68-69	22.416185365770392	26.82932442301807	27.90973672627604	22.844753484935495
70-71	22.363603693837543	26.660715812472198	27.85543844013044	23.12024205355982
72-73	22.446539184835444	26.73654203640406	27.83380917900276	22.983109599757732
74-75	22.399528880169868	26.960118804131078	27.672483322126357	22.9678689935727
76-77	22.5015134029895	26.735460205578953	27.627407804343363	23.13561858708819
78-79	22.412960409096403	26.853870476072615	27.585782272357147	23.147386842473836
80-81	22.403125561026073	26.970186407723197	27.503239282630343	23.12344874862039
82-83	22.499834644644483	26.842828286769166	27.6892495853924	22.96808748319395
84-85	22.514920451942512	26.86467739499524	27.507231052767217	23.113171100295034
86-87	22.337455982789663	26.930546915267193	27.57079863290233	23.16119846904081
88-89	22.434200409684852	26.738116403716212	27.527432593339245	23.30025059325969
90-91	22.413923958034882	26.754515639382838	27.554285209185508	23.277275193396775
92-93	22.4551026877427	26.70589974457605	27.54575075743259	23.293246810248657
94-95	22.202775861335862	26.640782889762754	27.73012962157778	23.4263116273236
96-97	22.331023333276438	26.56504129470545	27.66774221472271	23.4361931572954
98-99	22.243841255302627	26.705935514252992	27.471357998538465	23.57886523190591
100	22.30011981822743	26.517959170361642	27.547676590151866	23.634244421259062
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	300.0
1	199.5
2	323.0
3	510.5
4	318.5
5	262.0
6	571.0
7	1341.0
8	2664.0
9	3344.0
10	2433.0
11	1562.5
12	1591.0
13	1795.5
14	2032.0
15	2373.5
16	2790.5
17	3157.0
18	3586.5
19	4063.5
20	4616.5
21	5620.5
22	7017.5
23	8730.5
24	10924.0
25	13788.0
26	17691.5
27	21924.0
28	26522.0
29	32106.5
30	38395.0
31	45334.0
32	53095.0
33	59374.5
34	64170.0
35	70101.5
36	77448.5
37	85854.5
38	92346.0
39	95844.5
40	98847.0
41	102521.5
42	107467.0
43	113432.0
44	119093.5
45	125533.5
46	131105.5
47	132643.5
48	132895.5
49	131738.5
50	126802.5
51	122952.0
52	119661.5
53	114739.5
54	109776.0
55	101263.0
56	91700.0
57	81893.5
58	71874.0
59	63161.5
60	52387.0
61	41549.5
62	33082.5
63	24838.5
64	17410.5
65	12401.0
66	8919.5
67	6531.0
68	4802.5
69	3585.5
70	2712.5
71	1941.5
72	1420.5
73	1178.0
74	946.0
75	710.0
76	499.5
77	319.5
78	199.0
79	106.5
80	65.5
81	43.5
82	32.0
83	25.5
84	17.0
85	9.5
86	4.5
87	2.0
88	1.5
89	1.0
90	1.5
91	1.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006156997336031846
2	0.09631738406861699
3	4.5720277247761234E-4
4	0.0
5	0.0408434476746667
6	0.005486433269731347
7	3.048018483184082E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.334032345572144E-4
24-25	0.0
26-27	0.0
28-29	1.524009241592041E-5
30-31	1.2192073932736329E-4
32-33	0.0018440511823263695
34-35	0.0014020885022646777
36-37	8.077248980437817E-4
38-39	7.772447132119409E-4
40-41	0.0015849696112557226
42-43	7.772447132119409E-4
44-45	5.94363604220896E-4
46-47	3.9624240281393064E-4
48-49	8.22964990459702E-4
50-51	7.772447132119409E-4
52-53	0.001645929980919404
54-55	0.0015392493340079614
56-57	9.601258222029859E-4
58-59	0.0027889369121134352
60-61	0.0019507318292378126
62-63	0.001158247023609951
64-65	0.004983510220005974
66-67	0.004892069665510451
68-69	0.003901463658475625
70-71	0.001783090812662688
72-73	0.005166391328997019
74-75	0.0038405032888119434
76-77	0.00408434476746667
78-79	8.686852677074634E-4
80-81	3.048018483184082E-5
82-83	2.1336129382288577E-4
84-85	1.371608317432837E-4
86-87	3.048018483184082E-5
88-89	1.676410165751245E-4
90-91	0.0
92-93	0.0
94-95	1.524009241592041E-5
96-97	3.6576221798208984E-4
98-99	5.334032345572144E-4
100	5.181631421412939E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3280820.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.59194442685583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.33929100431531	40.50976538544968
2	16.248519809761184	18.715676998048647
3	6.126616659217337	10.585312984868821
4	2.8400806195282953	6.542630608306556
5	1.5151148911247923	4.362920630498038
6	0.8776304542428938	3.032666660884378
7	0.529133663350404	2.1331685573838577
8	0.355705614807665	1.6388622400258905
9	0.2515566231747733	1.3038871555880152
>10	0.8859685925285002	7.950747990392911
>50	0.021246769966491642	0.8428344059836662
>100	0.007681664547376187	0.7552194491536217
>500	0.0010498463697094724	0.41161141535551526
>1k	2.4227223916359152E-4	0.34863719429419954
>5k	8.07574130545305E-5	0.3974724469634777
>10k+	8.07574130545305E-5	0.46858587680283614
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10075	0.30708786218079626	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8546	0.26048365957291164	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4762	0.145146640169226	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.048018483184082E-5	3.048018483184082E-5
2	0.0	0.0	0.0	6.096036966368164E-5	3.048018483184082E-5
3	0.0	0.0	0.0	6.096036966368164E-5	3.048018483184082E-5
4	0.0	0.0	0.0	1.2192073932736329E-4	6.096036966368164E-5
5	0.0	0.0	0.0	1.2192073932736329E-4	6.096036966368164E-5
6	0.0	0.0	0.0	1.2192073932736329E-4	9.144055449552246E-5
7	0.0	0.0	0.0	1.2192073932736329E-4	9.144055449552246E-5
8	0.0	0.0	0.0	1.2192073932736329E-4	9.144055449552246E-5
9	0.0	0.0	0.0	1.2192073932736329E-4	1.2192073932736329E-4
10-11	0.0	0.0	0.0	1.8288110899104492E-4	1.2192073932736329E-4
12-13	0.0	0.0	0.0	2.1336129382288575E-4	1.524009241592041E-4
14-15	0.0	0.0	0.0	2.4384147865472657E-4	2.2860138623880617E-4
16-17	0.0	0.0	0.0	2.4384147865472657E-4	2.4384147865472657E-4
18-19	0.0	0.0	0.0	2.4384147865472657E-4	2.743216634865674E-4
20-21	0.0	0.0	0.0	3.6576221798208984E-4	3.048018483184082E-4
22-23	0.0	0.0	0.0	6.400838814686572E-4	4.1148249522985107E-4
24-25	0.0	0.0	0.0	0.001005846099450747	4.8768295730945314E-4
26-27	0.0	0.0	0.0	0.001965971921653733	5.486433269731348E-4
28-29	0.0	0.0	0.0	0.006126517151200005	5.486433269731348E-4
30-31	0.0	0.0	0.0	0.014584768442035833	5.486433269731348E-4
32-33	0.0	0.0	0.0	0.033299601928786095	5.94363604220896E-4
34-35	0.0	0.0	0.0	0.06230149779628264	6.400838814686572E-4
36-37	0.0	0.0	0.0	0.10907334142074238	6.553239738845777E-4
38-39	0.0	0.0	0.0	0.17035375302515834	6.858041587164184E-4
40-41	0.0	0.0	0.0	0.23638907346334148	7.77244713211941E-4
42-43	0.0	0.0	0.0	0.3003974616102072	7.924848056278613E-4
44-45	0.0	0.0	0.0	0.3803012661468779	8.229649904597021E-4
46-47	0.0	0.0	0.0	0.4766796105851586	9.601258222029859E-4
48-49	0.0	0.0	0.0	0.5770813394212422	0.0010363262842825879
50-51	0.0	0.0	0.0	0.6786717954657677	0.0010363262842825879
52-53	0.0	0.0	0.0	0.773388969830713	0.0012039673008577122
54-55	0.0	0.0	0.0	0.875634749849123	0.0014020885022646777
56-57	0.0	0.0	0.0	0.9966106034466993	0.0014325686870965185
58-59	0.0	0.0	0.0	1.1282392816430038	0.0015087691491761205
60-61	0.0	0.0	0.0	1.2651867520924647	0.0016459299809194043
62-63	0.0	0.0	0.0	1.4001834907126876	0.0017678507202467677
64-65	0.0	0.0	0.0	1.5394474552093684	0.0017678507202467677
66-67	0.0	0.0	0.0	1.6947287568351814	0.001783090812662688
68-69	0.0	0.0	0.0	1.8683591297297628	0.0017983309050786083
70-71	1.524009241592041E-5	0.0	0.0	2.045647124804165	0.0018288110899104492
72-73	3.048018483184082E-5	0.0	0.0	2.227156625477777	0.0020726525685651758
74-75	3.048018483184082E-5	0.0	0.0	2.4058619491468596	0.0020726525685651758
76-77	3.048018483184082E-5	0.0	0.0	2.5957992209264757	0.0021336129382288575
78-79	3.048018483184082E-5	0.0	0.0	2.8046646874866648	0.002164093123060698
80-81	3.048018483184082E-5	0.0	0.0	3.0317268243914635	0.002255533677556221
82-83	3.048018483184082E-5	0.0	0.0	3.265006919001957	0.002377454416883584
84-85	3.048018483184082E-5	0.0	0.0	3.4958028785486555	0.002377454416883584
86-87	3.048018483184082E-5	0.0	0.0	3.732557714229979	0.002377454416883584
88	3.048018483184082E-5	0.0	0.0	3.928286221127645	0.002407934601715425
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5890	0.0	59.616474	1
GTATCAA	11000	0.0	43.475418	1
ATCAACG	13940	0.0	33.93185	3
CAACGCA	14055	0.0	33.654213	5
TCAACGC	14180	0.0	33.390705	4
TATCAAC	14375	0.0	33.06865	2
AACGCAG	14925	0.0	31.80538	6
ACGCAGA	16525	0.0	28.553064	7
CGCAGAG	16805	0.0	27.993423	8
GCAGAGT	18890	0.0	24.704596	9
TACATGG	9585	0.0	24.060625	2
GTACATG	9755	0.0	23.933	1
ACATGGG	9590	0.0	22.602406	3
GAGTACT	13275	0.0	22.037651	12-13
AGAGTAC	16800	0.0	21.95942	10-11
CAGAGTA	18180	0.0	21.48165	10-11
CATGGGG	7110	0.0	21.426327	4
GTACTAG	860	0.0	20.798302	1
AGTACTT	13775	0.0	20.435991	12-13
GTACTTT	14610	0.0	19.83094	14-15
>>END_MODULE