FastQCFastQC Report
Mon 16 Jan 2017
ERR1146490_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146490_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4184006
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55800.036.566031
GTATCAA85000.033.6284521
CAACGCA94050.028.9304455
ATCAACG95650.028.545793
TCAACGC101100.026.9598454
AACGCAG102200.026.9439546
TATCAAC108200.025.7559952
TATTCGC1901.3371391E-824.7332829
GTACATG129100.024.0711841
CGCAGAG113000.023.746148
ACGCAGA113800.023.74247
TACATGG129500.023.4430262
GTACTAG14700.023.0270021
ACATGGG127850.022.6795983
CATGGGG91450.020.914684
GCAGAGT130500.019.6255269
TACCCCG3707.2759576E-1219.0513115
GTCCTAC13200.018.8766171
GCATCGT12750.018.0601448
AGAGTAC109550.017.73774710-11
Produced by FastQC (version 0.11.5)