FastQCFastQC Report
Mon 16 Jan 2017
ERR1146490_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146490_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4184006
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176020.4206972934551241No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156520.37409124174296116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92980.22222721477932872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98250.053.122681
GTATCAA172400.041.4122921
ATCAACG217850.032.3903663
CAACGCA218200.032.3811155
TATCAAC224950.031.651362
TCAACGC224900.031.4582524
AACGCAG229650.030.9226386
ACGCAGA254200.027.7136827
CGCAGAG254850.027.5876778
GCAGAGT279800.024.7077459
GTACATG126000.023.867741
TACATGG123000.023.5675812
ACATGGG121500.022.7893413
GAGTACT197400.022.53417412-13
AGAGTAC255700.021.9636910-11
CAGAGTA275050.021.29848910-11
TGATCGC7100.021.181659
GTACTTT208600.021.02013614-15
GTACTAG16150.020.9817141
AGTACTT205750.020.91157212-13
Produced by FastQC (version 0.11.5)