##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146490_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4184006
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5238668395791	34.0	31.0	34.0	31.0	34.0
2	32.594522569996315	34.0	31.0	34.0	31.0	34.0
3	32.68406594063202	34.0	31.0	34.0	31.0	34.0
4	36.18165294218029	37.0	37.0	37.0	35.0	37.0
5	36.2000912522592	37.0	37.0	37.0	35.0	37.0
6	36.27467670935462	37.0	37.0	37.0	35.0	37.0
7	36.262823953885345	37.0	37.0	37.0	35.0	37.0
8	36.271269687471765	37.0	37.0	37.0	35.0	37.0
9	38.08074175801851	39.0	38.0	39.0	37.0	39.0
10-11	38.041549534106785	39.0	38.0	39.0	35.0	39.0
12-13	38.01618664026772	39.0	38.0	39.0	35.0	39.0
14-15	39.466606405440146	41.0	39.0	41.0	37.0	41.0
16-17	39.46662158228263	41.0	39.0	41.0	36.5	41.0
18-19	39.527301108076806	41.0	39.5	41.0	37.0	41.0
20-21	39.564819457715885	41.0	40.0	41.0	37.0	41.0
22-23	39.52255649250981	41.0	39.0	41.0	37.0	41.0
24-25	39.50889721477455	41.0	39.0	41.0	36.5	41.0
26-27	39.43499614962312	41.0	39.0	41.0	36.0	41.0
28-29	39.344262054117515	41.0	39.0	41.0	36.0	41.0
30-31	39.23045652898203	41.0	39.0	41.0	35.5	41.0
32-33	39.093837341533444	41.0	39.0	41.0	35.0	41.0
34-35	39.012275914518284	41.0	39.0	41.0	35.0	41.0
36-37	38.92089148533726	41.0	39.0	41.0	35.0	41.0
38-39	38.82081705905776	40.0	38.0	41.0	35.0	41.0
40-41	38.73589007759549	40.0	38.0	41.0	35.0	41.0
42-43	38.64312646779187	40.0	38.0	41.0	35.0	41.0
44-45	38.499185828127395	40.0	38.0	41.0	35.0	41.0
46-47	38.26202866343882	40.0	38.0	41.0	34.0	41.0
48-49	38.0499631214678	40.0	38.0	41.0	33.5	41.0
50-51	37.911167192398864	40.0	38.0	41.0	33.0	41.0
52-53	37.7960184569525	40.0	37.5	41.0	33.0	41.0
54-55	37.671032259513964	40.0	37.0	41.0	33.0	41.0
56-57	37.4943362891927	40.0	37.0	41.0	33.0	41.0
58-59	37.30388675350848	40.0	36.0	41.0	33.0	41.0
60-61	37.13438054343135	39.0	36.0	41.0	32.5	41.0
62-63	36.890870256878216	39.0	35.0	41.0	32.0	41.0
64-65	36.60570360080746	39.0	35.0	41.0	32.0	41.0
66-67	36.35445264657842	38.0	35.0	40.5	31.5	41.0
68-69	36.042034117541895	37.0	35.0	40.0	31.0	41.0
70-71	35.750676385263304	37.0	35.0	39.5	31.0	41.0
72-73	35.41570578531675	36.0	35.0	39.0	31.0	41.0
74-75	35.07900263527347	36.0	35.0	39.0	31.0	41.0
76-77	34.76076050082146	35.0	35.0	37.5	31.0	39.5
78-79	34.45551547966231	35.0	35.0	37.0	30.0	39.0
80-81	34.17291084190606	35.0	35.0	37.0	30.0	39.0
82-83	33.906167199569026	35.0	34.0	36.0	30.0	38.0
84-85	33.68518866846749	35.0	34.0	36.0	30.0	37.0
86-87	33.487773559598146	35.0	34.0	36.0	30.0	37.0
88-89	33.31706371835987	35.0	34.0	35.0	29.5	36.5
90-91	33.22048641899653	35.0	34.0	35.0	29.0	36.0
92-93	33.13273116721152	35.0	34.0	35.0	29.0	36.0
94-95	33.052029323093706	35.0	34.0	35.0	29.0	36.0
96-97	33.000394597904496	35.0	34.0	35.0	29.0	36.0
98-99	32.94422163830549	35.0	34.0	35.0	29.0	35.0
100	32.39984263884899	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.31071964799058804
1101	2	0.2863989067364727
1101	3	0.23577079463213835
1101	4	0.15309476481629503
1101	5	0.21643115372582145
1101	6	0.14238983277836326
1101	7	0.16608520731117693
1101	8	0.17479378908060283
1101	9	0.2043042639277175
1101	10-11	0.22469514563103843
1101	12-13	0.2065290414577703
1101	14-15	0.29419438230394945
1101	16-17	0.37042341933077694
1101	18-19	0.30436986532070875
1101	20-21	0.25926422425205686
1101	22-23	0.3160080570826267
1101	24-25	0.27509755152374993
1101	26-27	0.2729284000865775
1101	28-29	0.2991253607196285
1101	30-31	0.3007777706161434
1101	32-33	0.29229568005006
1101	34-35	0.3133702242327274
1101	36-37	0.33400904480089366
1101	38-39	0.3400912151919755
1101	40-41	0.2718358437628794
1101	42-43	0.262741823392183
1101	44-45	0.3058700998682653
1101	46-47	0.2661741711800474
1101	48-49	0.32897247144395436
1101	50-51	0.29527997429845954
1101	52-53	0.3175715805174164
1101	54-55	0.3032216946028541
1101	56-57	0.29867826912086315
1101	58-59	0.26911122502080076
1101	60-61	0.23019017447962398
1101	62-63	0.2172789869670808
1101	64-65	0.3010639038414027
1101	66-67	0.3397642930333262
1101	68-69	0.29693373725358896
1101	70-71	0.33160497621191354
1101	72-73	0.3046033833144861
1101	74-75	0.24180182344021262
1101	76-77	0.2226040261461435
1101	78-79	0.2777356201866681
1101	80-81	0.27418865667590353
1101	82-83	0.25123425672093447
1101	84-85	0.19345821703513622
1101	86-87	0.2233659633167946
1101	88-89	0.2707669562397115
1101	90-91	0.20784835483149067
1101	92-93	0.19078898492017515
1101	94-95	0.21750740955916115
1101	96-97	0.1437574512115063
1101	98-99	0.13349795187627223
1101	100	0.17398195749374423
1102	1	0.3131674379464755
1102	2	0.3411270391398773
1102	3	0.28420192976061287
1102	4	0.1862506399637951
1102	5	0.1793137333937196
1102	6	0.20942266399276122
1102	7	0.21301932332806928
1102	8	0.20167508139574863
1102	9	0.27037895243670107
1102	10-11	0.2542792212421645
1102	12-13	0.28563032325838833
1102	14-15	0.39157801249527324
1102	16-17	0.38530629374029957
1102	18-19	0.3595171478003394
1102	20-21	0.3707364938342437
1102	22-23	0.3826642260419675
1102	24-25	0.3547452476245496
1102	26-27	0.36807440477694087
1102	28-29	0.41097718152947493
1102	30-31	0.44767312791098846
1102	32-33	0.4282607589462444
1102	34-35	0.45336968695023216
1102	36-37	0.5129176387704675
1102	38-39	0.540694947372657
1102	40-41	0.5437047022056092
1102	42-43	0.5247827125156803
1102	44-45	0.5063399431119464
1102	46-47	0.5062951467415999
1102	48-49	0.5944662971449972
1102	50-51	0.5532110176439389
1102	52-53	0.6038777474577941
1102	54-55	0.5472453566831206
1102	56-57	0.5650516209453897
1102	58-59	0.5645942632317471
1102	60-61	0.5252853100076393
1102	62-63	0.537944850654327
1102	64-65	0.5457766356964058
1102	66-67	0.48214737398778595
1102	68-69	0.4492462834439408
1102	70-71	0.3943749457202941
1102	72-73	0.3524397387566722
1102	74-75	0.361506685335371
1102	76-77	0.3468703433290372
1102	78-79	0.37515878281337933
1102	80-81	0.3806138081112067
1102	82-83	0.3717335528742325
1102	84-85	0.3505071507850843
1102	86-87	0.3513865834085408
1102	88-89	0.32952341226330617
1102	90-91	0.30819456000990186
1102	92-93	0.31754860167903587
1102	94-95	0.36257564111476626
1102	96-97	0.3808431957854239
1102	98-99	0.3730992278325189
1102	100	0.4147608882630678
1103	1	0.1872893367884103
1103	2	0.20018561055978523
1103	3	0.20250869594203635
1103	4	0.13532822932587862
1103	5	0.1421752866166628
1103	6	0.14142050336849366
1103	7	0.12061826010275922
1103	8	0.12557184359361884
1103	9	0.19018928359761134
1103	10-11	0.20217855253046224
1103	12-13	0.18454600482058936
1103	14-15	0.2395524525225312
1103	16-17	0.24222427241917188
1103	18-19	0.23218420186952926
1103	20-21	0.25957442274899734
1103	22-23	0.27255959197305657
1103	24-25	0.26733051746498404
1103	26-27	0.2652881018406319
1103	28-29	0.2695034325582242
1103	30-31	0.2728812030040544
1103	32-33	0.23273791213924966
1103	34-35	0.27164906618276774
1103	36-37	0.25166750480569533
1103	38-39	0.3056442151667227
1103	40-41	0.3048048209034562
1103	42-43	0.28957981655120335
1103	44-45	0.25888407312955763
1103	46-47	0.2807940173933048
1103	48-49	0.2644879387745789
1103	50-51	0.21052742777228417
1103	52-53	0.2747914554408837
1103	54-55	0.21800020266913123
1103	56-57	0.22913881280114623
1103	58-59	0.23997064104678145
1103	60-61	0.17598126963014948
1103	62-63	0.16370920813906764
1103	64-65	0.16943060570446988
1103	66-67	0.13715763113947332
1103	68-69	0.17174912956340904
1103	70-71	0.142069866989452
1103	72-73	0.09252501870766139
1103	74-75	0.1258447704581016
1103	76-77	0.0786026670566713
1103	78-79	0.10314243102290277
1103	80-81	0.17688429208294565
1103	82-83	0.16360139839717647
1103	84-85	0.1569409479229833
1103	86-87	0.1673811050076992
1103	88-89	0.12483231985895316
1103	90-91	0.13786201900657602
1103	92-93	0.10850289593240348
1103	94-95	0.16613816879239351
1103	96-97	0.12685616408076328
1103	98-99	0.19157980698498278
1103	100	0.18359330187348633
1104	1	0.13994036369327745
1104	2	0.14562158620429955
1104	3	0.15444386271578026
1104	4	0.11708291317000175
1104	5	0.051296446104743154
1104	6	0.06135970413773606
1104	7	0.10307623921419662
1104	8	0.07553858654433299
1104	9	0.10520508038956677
1104	10-11	0.12084013649519676
1104	12-13	0.11904607463219463
1104	14-15	0.162933399334662
1104	16-17	0.17427938828621592
1104	18-19	0.1704355270949094
1104	20-21	0.1841881819000477
1104	22-23	0.202745285950094
1104	24-25	0.1789773678091251
1104	26-27	0.21228880142773932
1104	28-29	0.24037314423689793
1104	30-31	0.17730730193010658
1104	32-33	0.13553396515390403
1104	34-35	0.14209914512075983
1104	36-37	0.19993535558512576
1104	38-39	0.17053421928432044
1104	40-41	0.1555300096798078
1104	42-43	0.17429636364327195
1104	44-45	0.15671109025908692
1104	46-47	0.09874824855909736
1104	48-49	0.12355841895708863
1104	50-51	0.1435147645960697
1104	52-53	0.03940308959764849
1104	54-55	0.05930842441728146
1104	56-57	0.08418462919795644
1104	58-59	0.05618785640572099
1104	60-61	0.05096632970061421
1104	62-63	0.05608322760467388
1104	64-65	0.08865845130595318
1104	66-67	0.02407661498155278
1104	68-69	0.09423702101868514
1104	70-71	0.011194889100551109
1104	72-73	0.015748138571922254
1104	74-75	-0.03283744623885809
1104	76-77	-4.7724535458115724E-4
1104	78-79	0.023878474002827943
1104	80-81	-0.01938956077869136
1104	82-83	0.011588350920007429
1104	84-85	-0.007412297471013574
1104	86-87	0.042081662850876
1104	88-89	0.024556754007974746
1104	90-91	-0.001792366333155826
1104	92-93	-0.06851286678119095
1104	94-95	-0.016172412208135256
1104	96-97	-0.05683213891363437
1104	98-99	-0.08666488828274765
1104	100	0.041191624384488534
1105	1	0.1491462871774729
1105	2	0.17542520277185503
1105	3	0.1935986457475849
1105	4	0.09768620773890291
1105	5	0.06387896520107006
1105	6	0.07352662354801254
1105	7	0.1125132153004671
1105	8	0.08999503508103146
1105	9	0.10182657596885036
1105	10-11	0.11780382939427625
1105	12-13	0.12489388324186734
1105	14-15	0.17817766523030798
1105	16-17	0.17439951308064394
1105	18-19	0.165635307338988
1105	20-21	0.15698969860464018
1105	22-23	0.18057894265295715
1105	24-25	0.1605863442070543
1105	26-27	0.17599436805148372
1105	28-29	0.18063511433211232
1105	30-31	0.1863469421578685
1105	32-33	0.18321747844451153
1105	34-35	0.18897371401200047
1105	36-37	0.19178014445962077
1105	38-39	0.21882850793817
1105	40-41	0.19055057362168526
1105	42-43	0.1280884027662097
1105	44-45	0.11698583375424931
1105	46-47	0.2746453495473915
1105	48-49	0.23816601910402824
1105	50-51	0.25881591298737305
1105	52-53	0.24554127137418646
1105	54-55	0.2528231778590708
1105	56-57	0.29774451902152776
1105	58-59	0.289599528978421
1105	60-61	0.29032793214194896
1105	62-63	0.2601834525490929
1105	64-65	0.21977017439266433
1105	66-67	0.23027704994697018
1105	68-69	0.2551401269452356
1105	70-71	0.26472779010609315
1105	72-73	0.17077818755716123
1105	74-75	0.1621945063491239
1105	76-77	0.17571732609793855
1105	78-79	0.18915466038375683
1105	80-81	0.1882509834955144
1105	82-83	0.2130500384421481
1105	84-85	0.16314842546074004
1105	86-87	0.14010982888321166
1105	88-89	0.16988779954746747
1105	90-91	0.14980191397399523
1105	92-93	0.11828439883316832
1105	94-95	0.17636303566864342
1105	96-97	0.14952577275954582
1105	98-99	0.12161345834890369
1105	100	0.1323868630523677
1106	1	0.11919325591554042
1106	2	0.11366666244655477
1106	3	0.1063635095922919
1106	4	0.09003144975692123
1106	5	0.03982159804815666
1106	6	0.050924280428652935
1106	7	0.04984883997248346
1106	8	0.06686910995903617
1106	9	0.11149568086046457
1106	10-11	0.05688089543469488
1106	12-13	0.0762852329480026
1106	14-15	0.10976802622184323
1106	16-17	0.05970533400493139
1106	18-19	0.1170592651438298
1106	20-21	0.06468911088520457
1106	22-23	0.12362545220797472
1106	24-25	0.07804053595771165
1106	26-27	0.04877088799751306
1106	28-29	0.11073827905569544
1106	30-31	0.09723353048227779
1106	32-33	0.0600195049520309
1106	34-35	0.0807498044137489
1106	36-37	0.0672909229481462
1106	38-39	0.04255844292464417
1106	40-41	0.052709956559858995
1106	42-43	0.0032625033166198136
1106	44-45	0.0211932918720521
1106	46-47	-0.0023570982665788165
1106	48-49	-0.055752540521332605
1106	50-51	-0.04066860663785121
1106	52-53	-0.06716723521521573
1106	54-55	-0.04132998573126656
1106	56-57	-0.04389020423950285
1106	58-59	-0.00685848508430098
1106	60-61	-0.051066439193519386
1106	62-63	-0.0613300034538895
1106	64-65	-0.04256688318326951
1106	66-67	-0.07995953393162836
1106	68-69	-0.0038803573697521188
1106	70-71	-0.1072464126109125
1106	72-73	-0.15092955496210436
1106	74-75	-0.051748182724466574
1106	76-77	-0.02479458563779957
1106	78-79	-0.08297280856962885
1106	80-81	-0.07195050240950707
1106	82-83	-0.05230333636803408
1106	84-85	-0.05420868841142834
1106	86-87	-0.09915554143582028
1106	88-89	-0.11182898388724993
1106	90-91	-0.07427489272715349
1106	92-93	-0.06020538690465571
1106	94-95	-0.13279492024978623
1106	96-97	-0.12925015244962168
1106	98-99	-0.1225765248790438
1106	100	-0.11278087278945037
1107	1	0.10437797796124215
1107	2	0.10823434090597317
1107	3	0.1213484474989528
1107	4	0.10228299440303346
1107	5	0.106102572968922
1107	6	0.07666464970874642
1107	7	0.08758189498934854
1107	8	0.10906242031913393
1107	9	0.14844967357080208
1107	10-11	0.1298281663207277
1107	12-13	0.13469587973141017
1107	14-15	0.21191701384827866
1107	16-17	0.2113772443027031
1107	18-19	0.17660755096588332
1107	20-21	0.1268010018928436
1107	22-23	0.18532946490543623
1107	24-25	0.16698315804318042
1107	26-27	0.16464690580347963
1107	28-29	0.17493205988199634
1107	30-31	0.1997516137066242
1107	32-33	0.10335208150785036
1107	34-35	0.07569885261654719
1107	36-37	0.09002661216575802
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2216	30-31	-0.40222722040441283
2216	32-33	-0.45821022369614894
2216	34-35	-0.45265538964829233
2216	36-37	-0.49295379965047914
2216	38-39	-0.4024447292838289
2216	40-41	-0.4351630493069436
2216	42-43	-0.5168005305556989
2216	44-45	-0.45208663357294654
2216	46-47	-0.4609506239802599
2216	48-49	-0.4951004977681279
2216	50-51	-0.4696404307678961
2216	52-53	-0.42719554126237114
2216	54-55	-0.4775964211086148
2216	56-57	-0.4658179798733144
2216	58-59	-0.4145107251689666
2216	60-61	-0.3708578000612306
2216	62-63	-0.3961828943111243
2216	64-65	-0.4142462609605744
2216	66-67	-0.3497634690092539
2216	68-69	-0.3544067811534717
2216	70-71	-0.28847807074922827
2216	72-73	-0.24060365995539712
2216	74-75	-0.2851789828921767
2216	76-77	-0.3192699245521382
2216	78-79	-0.4007966063220607
2216	80-81	-0.33011263085769826
2216	82-83	-0.27734955453821186
2216	84-85	-0.2696337323492699
2216	86-87	-0.25900825812941264
2216	88-89	-0.2275503854882004
2216	90-91	-0.2304636924334602
2216	92-93	-0.2317846124872318
2216	94-95	-0.2415264767251415
2216	96-97	-0.29801004332436065
2216	98-99	-0.2675460268001828
2216	100	-0.35020060765243954
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	17.0
8	87.0
9	193.0
10	385.0
11	415.0
12	330.0
13	385.0
14	518.0
15	801.0
16	1555.0
17	2874.0
18	5286.0
19	8360.0
20	11918.0
21	17657.0
22	25921.0
23	32129.0
24	31705.0
25	26789.0
26	23696.0
27	23938.0
28	27934.0
29	33708.0
30	41344.0
31	52554.0
32	67855.0
33	89893.0
34	129534.0
35	207414.0
36	367007.0
37	915626.0
38	1469259.0
39	565004.0
40	1914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.846854728066276	19.617949490169085	13.78376763374397	23.751428148020672
2	15.16931087449157	21.20655393229479	40.111031132207046	23.5131040610066
3	17.40683352656998	26.72875095990931	31.59833833215145	24.266077181369266
4	12.006627141548076	18.335657262441785	39.37468062904308	30.283034966967065
5	12.315142200514344	38.4274928964299	35.78685846704431	13.470506436011442
6	28.9155448710671	39.21765228762169	18.17412842690321	13.692674414407998
7	24.952210871590527	34.149520818086785	23.45159160861624	17.44667670170645
8	25.247286930276868	35.065269982882434	21.110031868979156	18.577411217861542
9	26.29377204525997	14.798473042342675	20.879726271903053	38.0280286404943
10-11	23.834131691015738	26.43429526630698	29.39485507429961	20.336717968377673
12-13	25.252628700819262	24.634309797834895	28.987948392043418	21.125113109302422
14-15	22.35906351416771	25.50085103896304	27.420878113510184	24.719207333359066
16-17	21.053913402609844	28.344282967089434	28.081042426803403	22.52076120349732
18-19	20.990170664191208	27.01983457958712	30.825386005660604	21.164608750561065
20-21	21.960162103018018	26.227209043199267	30.711057775729767	21.101571078052945
22-23	21.840379476046667	26.15850531772443	30.415397589782643	21.585717616446257
24-25	21.585461397521897	26.358387153364504	30.691064974572217	21.365086474541386
26-27	21.442356918226217	26.551145003138142	30.4225185145528	21.583979564082842
28-29	21.297981395574503	26.718530058942335	30.490478678978477	21.493009866504682
30-31	21.78633821864983	26.40299636675474	30.283170377250197	21.52749503734524
32-33	21.66557100581427	26.642520301195393	30.030221596760075	21.661687096230253
34-35	21.806830202875467	26.588189713729577	30.263284946794244	21.34169513660071
36-37	22.02694095194391	26.36244567055413	30.080318549509855	21.53029482799211
38-39	21.852520231679453	26.475485427060253	30.073323891743502	21.598670449516792
40-41	21.76311344846167	26.408400503879182	30.183562644727534	21.644923402931614
42-43	21.886837593681978	26.414978218050848	29.784243143292887	21.91394104497428
44-45	21.87111576431003	26.738622882832004	29.611426155410182	21.778835197447787
46-47	22.195724364278465	26.75601687003733	28.97372266542001	22.074536100264197
48-49	22.34821895276043	26.84407946422191	28.685356566763964	22.122345016253696
50-51	22.28181341144733	27.054552607385734	28.47236853127908	22.19126544988785
52-53	22.1903755789997	27.2097472717104	28.430343169357116	22.16953397993279
54-55	22.14927250251677	27.20246170348849	28.342860256811058	22.305405537183685
56-57	22.39109046190749	27.1402333503945	28.239728554167094	22.228947633530918
58-59	22.146078176660293	27.03562695228332	28.506115013254433	22.31217985780195
60-61	22.102981237082233	27.258735383764137	28.450943404118266	22.187339975035357
62-63	22.108816831910936	27.12160675628278	28.411223106353255	22.35835330545303
64-65	21.91376032104747	27.01025740922438	28.513338934295458	22.562643335432696
66-67	22.040386389393113	27.120468290808102	28.269588213902136	22.569557105896646
68-69	22.053229530840426	27.0279501405765	28.37385389461316	22.54496643396992
70-71	22.10601968651248	26.942906914921267	28.277764174141996	22.67330922442426
72-73	22.21379680988792	26.935123010422537	28.266146944210508	22.584933235479042
74-75	22.199465396512323	27.226272653037032	28.06516536150867	22.509096588941976
76-77	22.343631258057048	26.972397224413523	27.951128109332192	22.732843408197237
78-79	22.188386681671577	27.140545083627043	27.986716423314878	22.6843518113865
80-81	22.256629712440592	27.19760134129859	27.8977797588674	22.64798918739342
82-83	22.4218453389828	26.992906660630965	28.215386464923302	22.36986153546293
84-85	22.50288659108829	27.147077641711842	27.788867641284025	22.561168125915838
86-87	22.227303743661874	27.325517058416516	27.812263608671593	22.634915589250014
88-89	22.44820770003218	27.020906212733735	27.709482479127907	22.821403608106174
90-91	22.22664116638456	27.07124463970654	27.89820330085569	22.80391089305321
92-93	22.346000459846376	27.063166257409765	27.860332896272137	22.73050038647172
94-95	22.018278895665887	27.02313608335362	28.032981792208446	22.925603228772047
96-97	22.243573449796315	26.891241746366294	27.877166547103776	22.988018256733614
98-99	22.094601950509603	27.091203873055424	27.70426795421158	23.10992622222339
100	22.17522810246237	26.83844908251125	27.774257733375556	23.212065081650824
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	749.0
1	526.0
2	733.0
3	1137.0
4	730.0
5	514.0
6	1163.5
7	3013.5
8	5785.5
9	6905.5
10	4790.0
11	2657.0
12	2450.5
13	2742.0
14	3137.5
15	3652.0
16	4319.0
17	5036.5
18	5695.0
19	6442.0
20	7407.0
21	8798.5
22	10953.0
23	13417.5
24	15798.0
25	19642.5
26	25569.5
27	31893.5
28	38412.0
29	46814.0
30	55682.5
31	64186.0
32	75687.0
33	83987.0
34	89164.0
35	95205.0
36	103481.0
37	116441.5
38	123673.0
39	124916.5
40	127549.5
41	130649.5
42	133609.0
43	139113.0
44	146017.5
45	154595.0
46	160968.0
47	163828.0
48	168186.5
49	164446.5
50	155756.5
51	151215.0
52	145799.5
53	140946.5
54	137202.5
55	126987.0
56	114188.5
57	101850.5
58	88822.5
59	76911.0
60	62867.0
61	49848.5
62	39305.0
63	28409.0
64	19315.5
65	12938.0
66	8702.0
67	6280.5
68	4414.5
69	2921.0
70	2010.5
71	1401.5
72	962.5
73	765.5
74	610.5
75	465.0
76	353.0
77	241.0
78	161.0
79	120.0
80	96.5
81	71.0
82	44.0
83	35.0
84	29.5
85	22.0
86	16.0
87	8.5
88	4.5
89	3.5
90	3.0
91	1.5
92	1.0
93	1.0
94	0.5
95	0.5
96	0.5
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006357543464325817
2	0.08931631551197584
3	4.0630916877270256E-4
4	0.0
5	0.038288664021992325
6	0.00389578791235003
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	5.9751348348926843E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.333642924986246E-4
24-25	0.0
26-27	0.0
28-29	3.5850809009356106E-5
30-31	1.3145296636763904E-4
32-33	0.001852291798816732
34-35	0.0014340323603742441
36-37	9.799221129224002E-4
38-39	8.484691465547611E-4
40-41	0.0015893858660814542
42-43	8.843199555641174E-4
44-45	6.333642924986246E-4
46-47	4.7801078679141475E-4
48-49	8.365188768849757E-4
50-51	8.484691465547611E-4
52-53	0.0015774355964116684
54-55	0.0017686399111282347
56-57	9.679718432526148E-4
58-59	0.0029397663387672004
60-61	0.00211519773155201
62-63	0.0011591761579691807
64-65	0.004804008407253718
66-67	0.0046725554408860795
68-69	0.00389578791235003
70-71	0.0018044907201375906
72-73	0.005604676475129338
74-75	0.003764334945982391
76-77	0.00405114141805724
78-79	0.0010157729219317563
80-81	2.3900539339570737E-5
82-83	2.5095566306549273E-4
84-85	1.1950269669785369E-4
86-87	2.3900539339570737E-5
88-89	2.5095566306549273E-4
90-91	0.0
92-93	0.0
94-95	1.1950269669785369E-5
96-97	3.7045835976334644E-4
98-99	5.377621351403415E-4
100	7.409167195266929E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4184006.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.85810935168165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.55542875832465	35.03296811848939
2	16.58464778924326	17.200969572274047
3	6.6650127633065	10.369048821297069
4	3.287391187550042	6.819115667428986
5	1.8317722064874853	4.74961216956996
6	1.1556513418930878	3.595793619618564
7	0.719627110467585	2.61229509709439
8	0.5009067221269133	2.0780860456839907
9	0.3643255285889883	1.7003909791059277
>10	1.2828734488252278	10.335081089293473
>50	0.033642076577500846	1.1796334281273213
>100	0.016494327544734212	1.5114489351045701
>500	0.0011928959271664287	0.41129534567998116
>1k	7.952639514386811E-4	0.649378974535403
>5k	7.95263951438681E-5	0.383457748331651
>10k+	1.590527902877362E-4	1.3714243883653177
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17602	0.4206972934551241	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15652	0.37409124174296116	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9298	0.22222721477932872	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.170161801871221E-5
2	0.0	0.0	0.0	0.0	7.170161801871221E-5
3	0.0	0.0	0.0	0.0	7.170161801871221E-5
4	0.0	0.0	0.0	0.0	7.170161801871221E-5
5	0.0	0.0	0.0	0.0	7.170161801871221E-5
6	0.0	0.0	0.0	0.0	1.4340323603742442E-4
7	0.0	0.0	0.0	0.0	1.912043147165659E-4
8	0.0	0.0	0.0	0.0	1.912043147165659E-4
9	0.0	0.0	0.0	0.0	3.346075507539903E-4
10-11	0.0	0.0	0.0	0.0	3.824086294331318E-4
12-13	0.0	0.0	0.0	2.3900539339570737E-5	5.377621351403416E-4
14-15	0.0	0.0	0.0	4.7801078679141475E-5	6.692151015079805E-4
16-17	0.0	0.0	0.0	9.560215735828295E-5	7.648172588662636E-4
18-19	0.0	0.0	0.0	9.560215735828295E-5	7.887177982058343E-4
20-21	0.0	0.0	0.0	1.3145296636763907E-4	8.604194162245465E-4
22-23	0.0	0.0	0.0	3.1070701141441956E-4	8.843199555641173E-4
24-25	0.0	1.1950269669785369E-5	0.0	8.12618337545405E-4	9.321210342432588E-4
26-27	0.0	2.3900539339570737E-5	0.0	0.0015296345177325272	9.799221129224002E-4
28-29	0.0	2.3900539339570737E-5	0.0	0.005174466767017064	0.0010277231916015416
30-31	0.0	2.3900539339570737E-5	0.0	0.014053517131667592	0.0010277231916015416
32-33	0.0	4.7801078679141475E-5	0.0	0.03170406543394058	0.0011472258882993954
34-35	0.0	4.7801078679141475E-5	0.0	0.05869972461798573	0.0011830766973087515
36-37	0.0	4.7801078679141475E-5	0.0	0.10040616576553667	0.0012547783153274636
38-39	0.0	4.7801078679141475E-5	0.0	0.15865178013607056	0.001362330742355532
40-41	0.0	4.7801078679141475E-5	0.0	0.22027932082315368	0.0014101318210346734
42-43	0.0	4.7801078679141475E-5	0.0	0.27976776323934527	0.0014340323603742441
44-45	0.0	4.7801078679141475E-5	0.0	0.3535487281806001	0.001457932899713815
46-47	0.0	4.7801078679141475E-5	0.0	0.44119200593880603	0.0014818334390533858
48-49	0.0	4.7801078679141475E-5	0.0	0.5346311644868578	0.0015057339783929565
50-51	0.0	4.7801078679141475E-5	0.0	0.6321931660709855	0.0016013361357512393
52-53	0.0	4.7801078679141475E-5	0.0	0.7269827050917231	0.0017088885627793077
54-55	0.0	4.7801078679141475E-5	0.0	0.8234094310572213	0.0017327891021188784
56-57	0.0	4.7801078679141475E-5	0.0	0.936112424312967	0.0017925404504678052
58-59	0.0	4.7801078679141475E-5	0.0	1.0605983834631212	0.0018403415291469466
60-61	0.0	4.7801078679141475E-5	0.0	1.194895514012169	0.0018522917988167322
62-63	0.0	4.7801078679141475E-5	0.0	1.3227036481305237	0.001912043147165659
64-65	0.0	4.7801078679141475E-5	0.0	1.4497230644506724	0.001912043147165659
66-67	0.0	4.7801078679141475E-5	0.0	1.5943213274550754	0.001912043147165659
68-69	0.0	4.7801078679141475E-5	0.0	1.7552914599070844	0.001912043147165659
70-71	0.0	4.7801078679141475E-5	0.0	1.929418839265527	0.0019717944955145858
72-73	0.0	4.7801078679141475E-5	0.0	2.105063902872032	0.002043496113533298
74-75	0.0	4.7801078679141475E-5	0.0	2.276825128835857	0.00211519773155201
76-77	0.0	4.7801078679141475E-5	0.0	2.4589950396820655	0.0021868993495707226
78-79	0.0	4.7801078679141475E-5	0.0	2.6606797408990333	0.002234700428249864
80-81	0.0	4.7801078679141475E-5	0.0	2.8795250293618126	0.00227055123725922
82-83	0.0	4.7801078679141475E-5	0.0	3.105612181244482	0.002366153394617503
84-85	0.0	4.7801078679141475E-5	0.0	3.320979941233354	0.002402004203626859
86-87	0.0	4.7801078679141475E-5	0.0	3.543147404664334	0.002509556630654927
88	0.0	4.7801078679141475E-5	0.0	3.7223655989021047	0.0026051587880132105
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9825	0.0	53.12268	1
GTATCAA	17240	0.0	41.412292	1
ATCAACG	21785	0.0	32.390366	3
CAACGCA	21820	0.0	32.381115	5
TATCAAC	22495	0.0	31.65136	2
TCAACGC	22490	0.0	31.458252	4
AACGCAG	22965	0.0	30.922638	6
ACGCAGA	25420	0.0	27.713682	7
CGCAGAG	25485	0.0	27.587677	8
GCAGAGT	27980	0.0	24.707745	9
GTACATG	12600	0.0	23.86774	1
TACATGG	12300	0.0	23.567581	2
ACATGGG	12150	0.0	22.789341	3
GAGTACT	19740	0.0	22.534174	12-13
AGAGTAC	25570	0.0	21.96369	10-11
CAGAGTA	27505	0.0	21.298489	10-11
TGATCGC	710	0.0	21.18165	9
GTACTTT	20860	0.0	21.020136	14-15
GTACTAG	1615	0.0	20.981714	1
AGTACTT	20575	0.0	20.911572	12-13
>>END_MODULE