FastQCFastQC Report
Mon 16 Jan 2017
ERR1146489_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146489_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2527049
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38350.037.7651561
GTATCAA60600.034.1417661
CAACGCA62100.030.8006175
ATCAACG64800.029.517263
TATCAAC74150.027.4431112
AACGCAG72650.027.1666416
GTACTAG14500.025.6191651
GTACATG101750.025.2789381
TATTCGC2051.0841177E-925.217129
TACATGG100350.024.9612582
ACGCAGA75700.024.9532227
CGCAGAG76150.024.685778
CGTTAAC4400.024.565859
ACGTTAA4450.024.2898278
TCAACGC80500.023.9356164
ACATGGG102250.023.8080083
CATGGGG79550.022.6854634
GCTATTG18300.022.0853358
AGGGCTA19750.021.8915925
GCATCGT16050.021.3749188
Produced by FastQC (version 0.11.5)