##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146489_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2527049
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.262712357378113	31.0	31.0	34.0	28.0	34.0
2	31.29590522384014	31.0	31.0	34.0	28.0	34.0
3	31.462011619086137	33.0	31.0	34.0	28.0	34.0
4	35.024147137629704	37.0	35.0	37.0	32.0	37.0
5	34.962648527986595	37.0	35.0	37.0	32.0	37.0
6	35.11626406927606	37.0	35.0	37.0	32.0	37.0
7	34.96051956254113	37.0	35.0	37.0	32.0	37.0
8	34.95701151817792	37.0	35.0	37.0	32.0	37.0
9	36.542026292327535	39.0	37.0	39.0	32.0	39.0
10-11	36.52021250874043	39.0	37.0	39.0	32.0	39.0
12-13	36.47358638475154	39.0	37.0	39.0	32.0	39.0
14-15	37.84524795522366	40.0	38.0	41.0	32.0	41.0
16-17	37.86849661403479	40.0	38.0	41.0	32.0	41.0
18-19	37.90195461188129	40.0	38.0	41.0	32.0	41.0
20-21	38.03057083578514	40.0	38.0	41.0	32.0	41.0
22-23	38.063654879663986	40.0	38.0	41.0	32.0	41.0
24-25	38.130226600275655	40.0	38.0	41.0	32.0	41.0
26-27	38.1900469678269	40.0	38.0	41.0	32.5	41.0
28-29	38.17399444965254	40.0	38.0	41.0	32.0	41.0
30-31	38.168965461294974	40.0	38.0	41.0	32.5	41.0
32-33	38.18491667553735	40.0	38.0	41.0	33.0	41.0
34-35	38.11718787407763	40.0	38.0	41.0	32.0	41.0
36-37	38.016511551616134	40.0	38.0	41.0	32.0	41.0
38-39	37.96172907608835	40.0	38.0	41.0	32.0	41.0
40-41	37.88750257711663	40.0	38.0	41.0	32.0	41.0
42-43	37.73280989011293	40.0	37.5	41.0	31.5	41.0
44-45	37.53816051845453	40.0	37.0	41.0	31.0	41.0
46-47	37.41044950058349	40.0	37.0	41.0	31.0	41.0
48-49	37.27814359753214	40.0	36.0	41.0	30.5	41.0
50-51	37.12394872438168	40.0	36.0	41.0	30.0	41.0
52-53	36.98530044332342	39.5	35.0	41.0	30.0	41.0
54-55	36.797518370241335	39.0	35.0	41.0	30.0	41.0
56-57	36.637084005889875	39.0	35.0	41.0	30.0	41.0
58-59	36.38707342042042	38.5	35.0	40.5	30.0	41.0
60-61	36.19448791851681	38.0	35.0	40.0	30.0	41.0
62-63	35.94550738826196	37.5	35.0	40.0	30.0	41.0
64-65	35.683805300174235	37.0	35.0	40.0	29.0	41.0
66-67	35.424921321272365	36.5	34.5	40.0	29.0	41.0
68-69	35.13573440799921	36.0	34.0	39.0	29.0	41.0
70-71	34.90819232234911	35.5	34.0	39.0	29.0	41.0
72-73	34.61739542842264	35.0	34.0	38.5	29.0	40.5
74-75	34.31694893925682	35.0	34.0	37.5	28.5	40.0
76-77	34.03810096282264	35.0	34.0	37.0	28.0	39.0
78-79	33.780926883491375	35.0	34.0	36.5	28.0	39.0
80-81	33.54073545863179	35.0	34.0	36.0	28.0	39.0
82-83	33.297752239865545	35.0	33.0	36.0	27.5	37.0
84-85	33.091456675355325	35.0	33.0	35.0	27.0	37.0
86-87	32.90452717774764	35.0	33.0	35.0	27.0	36.5
88-89	32.78125889129969	35.0	33.0	35.0	27.0	36.0
90-91	32.61890074153686	35.0	33.0	35.0	27.0	36.0
92-93	32.52361430269061	35.0	33.0	35.0	27.0	36.0
94-95	32.43526045597058	35.0	33.0	35.0	26.5	35.5
96-97	32.350740923504056	35.0	33.0	35.0	26.0	35.0
98-99	32.285687376857354	35.0	33.0	35.0	26.0	35.0
100	31.31206874104934	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.23496795140226823
1101	2	0.20473347989981505
1101	3	0.24022833203756733
1101	4	0.2897987032714795
1101	5	0.2174396872356823
1101	6	0.2599183792389894
1101	7	0.2761392497682351
1101	8	0.3215341900814508
1101	9	0.3411548074619972
1101	10-11	0.3953678677812533
1101	12-13	0.34962363846405253
1101	14-15	0.5009590133405268
1101	16-17	0.47102700246696116
1101	18-19	0.3790951802897453
1101	20-21	0.3453970119003529
1101	22-23	0.347524952991499
1101	24-25	0.267020067748156
1101	26-27	0.22974615673058452
1101	28-29	0.39687536865396567
1101	30-31	0.34324078178376993
1101	32-33	0.38094308023053003
1101	34-35	0.33150843528677854
1101	36-37	0.33281232847242137
1101	38-39	0.32389266362429936
1101	40-41	0.239742884044432
1101	42-43	0.26790751689126324
1101	44-45	0.2393812822199024
1101	46-47	0.24022183397956098
1101	48-49	0.2408782150503015
1101	50-51	0.2773899098273347
1101	52-53	0.24175789012866034
1101	54-55	0.25056039568448085
1101	56-57	0.18854134701487624
1101	58-59	0.19069341143915608
1101	60-61	0.1976149545371868
1101	62-63	0.25668088519103094
1101	64-65	0.21812864795797537
1101	66-67	0.20917946391178788
1101	68-69	0.1632445216800562
1101	70-71	0.1873730257786761
1101	72-73	0.20073374302118907
1101	74-75	0.1779335176187402
1101	76-77	0.22029397798585393
1101	78-79	0.2147033955056301
1101	80-81	0.20418840358005497
1101	82-83	0.13826369613257583
1101	84-85	0.15826026019161077
1101	86-87	0.17528346270423611
1101	88-89	0.13171019327586464
1101	90-91	0.1763038620357591
1101	92-93	0.1613352475731915
1101	94-95	0.1793365994199121
1101	96-97	0.1433216710944123
1101	98-99	0.18410951300392497
1101	100	0.1534647288057478
1102	1	0.2811038209107153
1102	2	0.2782376663113659
1102	3	0.37892606545654317
1102	4	0.2633147548451191
1102	5	0.32280961914108275
1102	6	0.33981004360784794
1102	7	0.3339257426805773
1102	8	0.35968019346321256
1102	9	0.400101405757475
1102	10-11	0.43843219765483354
1102	12-13	0.4172757574302324
1102	14-15	0.4658684862927842
1102	16-17	0.45511393783946374
1102	18-19	0.4308478342328286
1102	20-21	0.38297680856458527
1102	22-23	0.3839857880032653
1102	24-25	0.3810091924007537
1102	26-27	0.4538061924798029
1102	28-29	0.4509504506901294
1102	30-31	0.5219334242204141
1102	32-33	0.5420140352795002
1102	34-35	0.5256947719416374
1102	36-37	0.4981985463968215
1102	38-39	0.46900285688256105
1102	40-41	0.4648243683407358
1102	42-43	0.3976781447810609
1102	44-45	0.3845941130721613
1102	46-47	0.43219355389787495
1102	48-49	0.41883150218308174
1102	50-51	0.40289454877065367
1102	52-53	0.4510961570305554
1102	54-55	0.4210748470380068
1102	56-57	0.46598456028997504
1102	58-59	0.43529860605442394
1102	60-61	0.41900468825495807
1102	62-63	0.39156718165806836
1102	64-65	0.32623589443819156
1102	66-67	0.27094255998590455
1102	68-69	0.27960441215002874
1102	70-71	0.2683083119626346
1102	72-73	0.3001905078093259
1102	74-75	0.2840563551584623
1102	76-77	0.37642320142676766
1102	78-79	0.35228147743713834
1102	80-81	0.2710344503425901
1102	82-83	0.27668441152301426
1102	84-85	0.2702834043531297
1102	86-87	0.2581960623829289
1102	88-89	0.26450160865370265
1102	90-91	0.30353959265084995
1102	92-93	0.2378551955320347
1102	94-95	0.1653603451175556
1102	96-97	0.1561446198158265
1102	98-99	0.16330551818911232
1102	100	0.13714796287839448
1103	1	0.08782171971551733
1103	2	0.12806769600567947
1103	3	0.11685452548257658
1103	4	0.07400861676070036
1103	5	0.020777141718014036
1103	6	0.22652799453079808
1103	7	0.11562758258144612
1103	8	0.15945465218369037
1103	9	0.2355357518766965
1103	10-11	0.24372857401039738
1103	12-13	0.17966000628396017
1103	14-15	0.2341114663041779
1103	16-17	0.20792896923413906
1103	18-19	0.16406318511173623
1103	20-21	0.19091459773886044
1103	22-23	0.224966807877216
1103	24-25	0.19783149688277746
1103	26-27	0.1643058491515177
1103	28-29	0.22815970635178928
1103	30-31	0.15739988814748784
1103	32-33	0.12588842012250012
1103	34-35	0.13059459434905563
1103	36-37	0.13371321960952542
1103	38-39	0.13344375294985866
1103	40-41	0.11893870513080884
1103	42-43	0.09409743266476056
1103	44-45	0.01748561481746691
1103	46-47	0.048144703559039215
1103	48-49	-0.04282843472789466
1103	50-51	-0.04799712757044006
1103	52-53	0.04851963130438719
1103	54-55	0.1332233915736083
1103	56-57	0.0655435756218381
1103	58-59	0.09871277463317085
1103	60-61	-0.03002767320523958
1103	62-63	0.014529524994181031
1103	64-65	0.00798001518551672
1103	66-67	-0.005436062713897627
1103	68-69	-0.011128851084400537
1103	70-71	-0.019802757019640183
1103	72-73	-0.02023316060460445
1103	74-75	-0.04189444270521392
1103	76-77	-0.07085440718419278
1103	78-79	0.02606194537991513
1103	80-81	0.10042015980737773
1103	82-83	0.037999586894052584
1103	84-85	0.04372223784477569
1103	86-87	0.10451951802452442
1103	88-89	0.035553431029853755
1103	90-91	0.08451112702211816
1103	92-93	0.0562299693067132
1103	94-95	0.1448459911284985
1103	96-97	0.0634192707219654
1103	98-99	0.014024488021824766
1103	100	-0.059654067644597575
1104	1	0.11031991971994515
1104	2	0.0941768615908849
1104	3	0.14162584182836468
1104	4	0.06307449982943325
1104	5	4.71933805492597E-4
1104	6	0.07805664028538217
1104	7	0.05990633639607523
1104	8	0.20512056352853136
1104	9	0.1048260483217689
1104	10-11	0.21426240742243152
1104	12-13	0.18243023759232813
1104	14-15	0.13066571864886356
1104	16-17	0.14802889881956816
1104	18-19	0.1530482977677181
1104	20-21	0.21814460852635165
1104	22-23	0.10838701571262987
1104	24-25	0.16823794996921038
1104	26-27	0.1942073574948182
1104	28-29	0.19075542359865238
1104	30-31	0.14977632427773102
1104	32-33	0.16505728869831415
1104	34-35	0.21732642356930398
1104	36-37	0.20448188599653605
1104	38-39	0.21376650376524964
1104	40-41	0.20218293254479391
1104	42-43	0.09718893636689785
1104	44-45	0.03947390582150234
1104	46-47	0.034315042716379196
1104	48-49	0.05370409791433417
1104	50-51	0.02202728792531161
1104	52-53	-0.07296165412635958
1104	54-55	0.006948737535907412
1104	56-57	0.01728224168059711
1104	58-59	0.02824513418979535
1104	60-61	0.054088831588906316
1104	62-63	0.0428159266253374
1104	64-65	0.03096369921751574
1104	66-67	0.04269827859972253
1104	68-69	0.02206546236838136
1104	70-71	-0.05902878950890056
1104	72-73	-0.06351839095708556
1104	74-75	-0.08747109735927694
1104	76-77	-0.06498384127523593
1104	78-79	-0.0027653810901284714
1104	80-81	0.050681911945510194
1104	82-83	0.03589942692986625
1104	84-85	-0.022384317240693008
1104	86-87	-0.00806633877035523
1104	88-89	0.027376406753148785
1104	90-91	0.02725567377000715
1104	92-93	-0.05666251351374285
1104	94-95	-0.07636844608954618
1104	96-97	-0.07063734334958838
1104	98-99	-0.0587272104614911
1104	100	-0.07092358640715446
1105	1	0.1167422600351884
1105	2	0.1780819716329951
1105	3	0.11711952215729582
1105	4	0.23480347001238044
1105	5	0.22292857278554123
1105	6	0.26268721821021757
1105	7	0.2445031589492146
1105	8	0.1803227129038305
1105	9	0.27452103686779594
1105	10-11	0.4127049858870322
1105	12-13	0.4025769187136703
1105	14-15	0.5173877826110456
1105	16-17	0.44288784345987864
1105	18-19	0.34066466259642425
1105	20-21	0.3686820605923131
1105	22-23	0.32979094411627585
1105	24-25	0.39527453542013546
1105	26-27	0.3359920657341462
1105	28-29	0.27724478396162056
1105	30-31	0.33641454502808443
1105	32-33	0.35298202207697926
1105	34-35	0.28004246095485996
1105	36-37	0.2887191777605622
1105	38-39	0.35686843320144845
1105	40-41	0.30574084493621
1105	42-43	0.2676164004428827
1105	44-45	0.2546302841956489
1105	46-47	0.12245148080625512
1105	48-49	0.17847541729796035
1105	50-51	0.18438785959858706
1105	52-53	0.2471825093734381
1105	54-55	0.19337592707889684
1105	56-57	0.17162430899585956
1105	58-59	0.1800257220287662
1105	60-61	0.15199255886346208
1105	62-63	0.23357406666105618
1105	64-65	0.3100907564410491
1105	66-67	0.10982039422927414
1105	68-69	0.028766825708558486
1105	70-71	0.17451075627207757
1105	72-73	0.18536750137418778
1105	74-75	0.1148784448866138
1105	76-77	0.15338663379112916
1105	78-79	0.15757552090311577
1105	80-81	0.20474507650439477
1105	82-83	0.13952152263492934
1105	84-85	0.13992457904765843
1105	86-87	0.18481009115249236
1105	88-89	0.23548316416965065
1105	90-91	0.26303169332856413
1105	92-93	0.1600163443744549
1105	94-95	0.07966279294346634
1105	96-97	0.13372288852495728
1105	98-99	0.12627195046496098
1105	100	0.18192715896516987
1106	1	0.14335377397222615
1106	2	0.10108718604974953
1106	3	0.09775355996127644
1106	4	0.1301638241109302
1106	5	0.1079850321698359
1106	6	0.132032904988435
1106	7	0.15929744673857726
1106	8	0.18516665506787433
1106	9	0.1580738889345028
1106	10-11	0.18727276989877595
1106	12-13	0.2297479772896054
1106	14-15	0.1761301557489432
1106	16-17	0.16122212861871077
1106	18-19	0.16839899376327594
1106	20-21	0.13373099071125694
1106	22-23	0.12203666915466016
1106	24-25	0.19388798429902465
1106	26-27	0.21512568519154485
1106	28-29	0.23408513052341817
1106	30-31	0.18361889167042733
1106	32-33	0.16034455515600854
1106	34-35	0.19746615265891165
1106	36-37	0.12731614729489138
1106	38-39	0.06638139573231427
1106	40-41	0.07955731186924453
1106	42-43	0.04781956783967445
1106	44-45	0.04336267645315672
1106	46-47	0.10983139003436548
1106	48-49	-0.015113163970013943
1106	50-51	0.03901240280281826
1106	52-53	0.0711373858827713
1106	54-55	0.08102645717795554
1106	56-57	0.04011720803325858
1106	58-59	0.06348881878461299
1106	60-61	0.11845510144202365
1106	62-63	0.12729775250370068
1106	64-65	0.10126929906782323
1106	66-67	0.11054779402540049
1106	68-69	0.11439676089384676
1106	70-71	0.10190892797361784
1106	72-73	0.06510466728175146
1106	74-75	0.07558459535619022
1106	76-77	0.052811840480991634
1106	78-79	0.10885620342675395
1106	80-81	0.13523943213798617
1106	82-83	0.1768349912240481
1106	84-85	0.14632801038045784
1106	86-87	0.0868203855401859
1106	88-89	-0.017621347767686757
1106	90-91	0.10378290261107281
1106	92-93	0.1390016242789045
1106	94-95	0.018818124352790733
1106	96-97	0.057760176728805845
1106	98-99	0.03268545774842835
1106	100	-0.010950715946467682
1107	1	-0.02080943311229788
1107	2	-0.06634544903232609
1107	3	-0.0059745607529286815
1107	4	-0.009443321723793474
1107	5	-0.10238501296401381
1107	6	-0.0723524726202669
1107	7	0.04622676131353387
1107	8	-0.0529696085979765
1107	9	0.028506867335273967
1107	10-11	0.041886838538481186
1107	12-13	-0.007166210146380081
1107	14-15	-0.020886757885101304
1107	16-17	-0.07318768905830808
1107	18-19	-0.04005170687264581
1107	20-21	0.030880316870941726
1107	22-23	0.03701333768173498
1107	24-25	-0.014533372235810305
1107	26-27	0.04296286110943015
1107	28-29	0.009913637189008284
1107	30-31	0.006983968330985135
1107	32-33	0.09259226597026071
1107	34-35	0.07758064562306544
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2216	4	0.04700558613124173
2216	5	0.1724126340277934
2216	6	-2.5448534366745434E-4
2216	7	0.12426322925454514
2216	8	0.14125292190786354
2216	9	-0.03973790985441639
2216	10-11	-0.04385981934880334
2216	12-13	-0.09144843709339057
2216	14-15	-0.22758597863124663
2216	16-17	-0.17903108768409481
2216	18-19	-0.1992508731665268
2216	20-21	-0.20649672571743594
2216	22-23	-0.13217086680413104
2216	24-25	-0.25214497844370953
2216	26-27	-0.25637583702896904
2216	28-29	-0.2592896674773044
2216	30-31	-0.30133901907127125
2216	32-33	-0.2922244309383686
2216	34-35	-0.3060358395081124
2216	36-37	-0.182504571854885
2216	38-39	-0.22614865015384567
2216	40-41	-0.23427519837694177
2216	42-43	-0.1360084857432966
2216	44-45	-0.1108160296145897
2216	46-47	-0.02636678466252107
2216	48-49	-0.011971318523414709
2216	50-51	-0.05576739571471023
2216	52-53	-0.08221647442375257
2216	54-55	-0.06097871109909647
2216	56-57	-0.09849752648042909
2216	58-59	-0.025651309575899006
2216	60-61	-0.033731097220538686
2216	62-63	-0.0995353423958889
2216	64-65	-0.06906773268329403
2216	66-67	-0.14473896241216977
2216	68-69	-0.14128979612846848
2216	70-71	-0.22705411269604525
2216	72-73	-0.14543273164485981
2216	74-75	-0.1317405569140817
2216	76-77	-0.14140890095209357
2216	78-79	-0.17209613376293476
2216	80-81	-0.09977092495214634
2216	82-83	-0.041037578388333884
2216	84-85	-0.011942673818325034
2216	86-87	-0.06474550528358947
2216	88-89	-0.09116284289878251
2216	90-91	-0.10242090758635669
2216	92-93	-0.10023215300752497
2216	94-95	-0.06563107666438839
2216	96-97	-0.03405116981317491
2216	98-99	-0.029535766792399443
2216	100	0.00856618993114111
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	66.0
1	27.0
2	29.0
3	28.0
4	22.0
5	38.0
6	129.0
7	700.0
8	2015.0
9	2598.0
10	2691.0
11	2996.0
12	3499.0
13	4172.0
14	5407.0
15	6175.0
16	6418.0
17	6862.0
18	7704.0
19	8612.0
20	9473.0
21	10773.0
22	11998.0
23	14660.0
24	18577.0
25	23936.0
26	26558.0
27	24286.0
28	25440.0
29	32063.0
30	36618.0
31	38818.0
32	49135.0
33	66544.0
34	102830.0
35	161389.0
36	314987.0
37	642702.0
38	657460.0
39	198409.0
40	205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.72095225877162	20.881778571801902	13.358215219605215	24.039053949821266
2	15.24222578929372	21.655316414016692	39.353795448501195	23.748662348188393
3	17.797726021574654	27.0840415852548	30.555086135966164	24.563146257204384
4	12.487029120465534	18.23668065793525	39.54806329235156	29.728226929247658
5	13.199609458636374	38.438087835396395	34.93117044866744	13.431132257299794
6	28.706598637625042	38.79677722177457	18.936711145164328	13.55991299543606
7	25.441941101957	33.37291950025663	23.64884998953295	17.53628940825342
8	25.479487074971534	34.34257291191368	21.445325315136735	18.73261469797805
9	26.319428257319032	15.431891853600757	21.451352285098842	36.797327603981365
10-11	24.336377393853624	26.652071639107078	28.40786770129899	20.60368326574031
12-13	24.95571179792589	24.868827180341878	27.67964646211813	22.495814559614104
14-15	22.66839690946659	25.843542834392576	25.573380380740485	25.914679875400353
16-17	22.176833703626606	28.46889734394058	26.44913025273604	22.905138699696774
18-19	21.939696026499238	28.51594762680238	27.748534250939404	21.795822095758975
20-21	23.42578505207891	27.50651839724499	26.849108576898885	22.218587973777215
22-23	23.83407304694139	27.292652151778114	26.337821080917763	22.53545372036273
24-25	23.228686100248282	27.492930257075088	26.83332502297221	22.44505861970442
26-27	22.915131129969673	27.87502431300789	26.349997694839942	22.859846862182494
28-29	22.561828380388093	28.057266689725097	26.65318249849421	22.7277224313926
30-31	22.958674346601068	27.60460724828932	26.69159103379729	22.745127371312325
32-33	23.039228781162617	28.075231750128772	25.923384288976735	22.96215517973188
34-35	23.297873182469388	27.799036718669612	26.610330143502797	22.292759955358203
36-37	23.21620810793268	27.494763738951605	26.4626108005503	22.82641735256541
38-39	23.089828872319366	27.424658423418414	26.74041683801071	22.74509586625151
40-41	23.039647463761163	26.94647027404951	27.05721174120772	22.95667052098161
42-43	22.82214215344206	27.20754389874444	26.729380760825812	23.240933186987693
44-45	22.755577053357275	27.7778492465335	26.724474554561084	22.742099145548146
46-47	22.839507761073683	27.450591152611526	26.412410117826767	23.297490968488024
48-49	23.189428962407288	27.1294019105706	26.737459035309495	22.943710091712614
50-51	23.01319932064736	27.18408093329641	26.453109843389065	23.349609902667158
52-53	22.785999897477772	27.415056477276977	26.39628639959561	23.402657225649637
54-55	22.768592759383	27.396432756976154	26.405745243355977	23.429229240284872
56-57	23.316710549391125	27.050107064346683	26.388718992609412	23.24446339365278
58-59	22.878540944299317	27.05156945790928	26.893877465384758	23.176012132406644
60-61	22.90409830954999	27.401817622206543	26.549496704904097	23.144587363339372
62-63	23.21558529467358	27.177876893510184	26.129436086021546	23.47710172579469
64-65	22.441133147571005	27.211383887130186	26.484472325067976	23.86301064023083
66-67	23.12063113541194	26.94908068281954	26.37210751375433	23.55818066801419
68-69	22.734734087226098	26.94608111614819	26.598996969372475	23.72018782725324
70-71	22.856856130491504	26.843436757672407	26.46889936242342	23.83080774941267
72-73	23.247004232136923	26.984791463046292	26.571731573815775	23.196472731001005
74-75	23.052774946750404	27.399121681042395	26.4874505928267	23.060652779380504
76-77	22.98292878591834	27.18718559420481	26.302935222732167	23.526950397144685
78-79	22.948489260579688	27.290395445336195	26.28689758261108	23.474217711473035
80-81	22.87124763830455	27.220290638234278	26.691350033699624	23.21711168976155
82-83	23.25846259451872	26.564247391321043	27.288668205014314	22.888621809145924
84-85	23.452370231307974	27.148686107765585	26.208589855621785	23.190353805304657
86-87	22.6499552195233	27.4551329312162	26.584165029663975	23.31074681959653
88-89	23.052868187074758	26.80242261972031	26.516507416952283	23.62820177625265
90-91	23.15204031343458	26.720990576774252	26.61755479068405	23.50941431910712
92-93	23.462089462668995	26.772950049699723	26.626087465971228	23.138873021660054
94-95	22.56192943969917	26.80211497614585	27.084944442586057	23.551011141568925
96-97	22.843307916495544	26.810491991469444	26.732766938648272	23.61343315338674
98-99	22.664552884629657	27.236536762093632	26.55767230638756	23.54123804688915
100	23.15173187435727	26.656686476631393	26.102801762867834	24.088779886143502
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	935.0
1	679.0
2	474.5
3	557.5
4	537.5
5	432.0
6	367.5
7	358.5
8	385.5
9	402.5
10	338.5
11	278.0
12	280.0
13	305.5
14	347.0
15	417.0
16	457.5
17	543.5
18	709.0
19	878.5
20	1147.0
21	1626.0
22	2503.0
23	3404.0
24	4339.0
25	5889.0
26	8893.0
27	12114.5
28	14885.0
29	19484.0
30	25649.5
31	31594.0
32	38414.0
33	45256.0
34	51644.5
35	56512.0
36	61748.5
37	70708.5
38	74558.5
39	74770.5
40	73728.5
41	71224.0
42	71061.5
43	73898.5
44	79368.5
45	89033.5
46	98125.0
47	104974.0
48	108922.0
49	106117.0
50	102978.0
51	104284.5
52	103950.0
53	101366.0
54	98931.5
55	90027.5
56	81257.0
57	69785.0
58	57660.0
59	51899.5
60	43063.5
61	33372.0
62	26181.5
63	19751.0
64	14269.5
65	9563.0
66	6527.0
67	4637.5
68	3385.5
69	2487.0
70	2077.5
71	1677.0
72	1254.0
73	1076.0
74	875.5
75	720.0
76	632.5
77	519.5
78	408.5
79	321.5
80	250.0
81	198.0
82	172.5
83	147.0
84	109.0
85	77.5
86	67.0
87	56.5
88	40.5
89	35.5
90	33.0
91	28.5
92	21.0
93	12.5
94	9.0
95	8.5
96	5.5
97	6.5
98	5.5
99	3.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06003049406639919
2	0.009220240683896513
3	0.011396692347477236
4	0.009299384380753993
5	0.01187155452862212
6	0.011277976802191015
7	0.002849173086869309
8	0.005144340295736252
9	0.007043789020315791
10-11	0.006371067597027205
12-13	0.005322413613665584
14-15	0.009338956229182734
16-17	0.00882452219960911
18-19	0.007281220110888233
20-21	0.006430425369670314
22-23	0.0022753812846525727
24-25	0.003284463419585453
26-27	0.004333117402947074
28-29	0.005342199537879954
30-31	0.008092443003677412
32-33	0.009635745092398288
34-35	0.010644827227331169
36-37	0.017292897763359558
38-39	0.018044762883505622
40-41	0.01755011477814637
42-43	0.024138827541531644
44-45	0.027403505036902726
46-47	0.029896531487913373
48-49	0.031182616561847436
50-51	0.0324687016357815
52-53	0.030371393669058257
54-55	0.0365841738723705
56-57	0.03949270473188292
58-59	0.03648524425129865
60-61	0.03745475453780279
62-63	0.03250827348421024
64-65	0.034902370314149035
66-67	0.03699967828087227
68-69	0.039749921746669734
70-71	0.03955206250452603
72-73	0.035693807282723845
74-75	0.03852319444537878
76-77	0.03777132932523271
78-79	0.03699967828087227
80-81	0.04180765786496423
82-83	0.03806811818844826
84-85	0.034486865905647264
86-87	0.033101851210641346
88-89	0.03068796845648818
90-91	0.0333986400738569
92-93	0.03474408292043407
94-95	0.03832533520323508
96-97	0.033101851210641346
98-99	0.03052968106277322
100	0.028135584232834424
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2527049.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.52102606528046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.22613195758167	35.150803229198296
2	14.78722616884717	15.23706129756745
3	6.582845331355196	10.174648377013826
4	3.4187160900956624	7.045430431504493
5	2.0838092319776145	5.367999487789538
6	1.329579226161067	4.110077158013987
7	0.8648872690461171	3.1191915672437966
8	0.6003447227825951	2.4744300888508524
9	0.42826702289390045	1.9858280804475041
>10	1.638012708723117	13.150668461071074
>50	0.028787681050130763	1.0026687284374802
>100	0.011260123743714495	1.1012750250146728
>500	0.0	0.0
>1k	1.3246574210850414E-4	0.07991806784708815
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	1.187155452862212E-4	0.0
22-23	0.0	0.0	0.0	5.144340295736252E-4	0.0
24-25	0.0	0.0	0.0	0.0013454428465771735	0.0
26-27	0.0	0.0	0.0	0.0027700293900118278	0.0
28-29	0.0	0.0	0.0	0.007518651201460676	0.0
30-31	0.0	0.0	0.0	0.02067629080401686	0.0
32-33	0.0	0.0	0.0	0.046872854463843004	0.0
34-35	0.0	0.0	0.0	0.08901687304045153	0.0
36-37	0.0	0.0	0.0	0.1521735431327212	0.0
38-39	0.0	0.0	0.0	0.23701558616394064	0.0
40-41	0.0	0.0	0.0	0.32557738294746164	0.0
42-43	0.0	0.0	0.0	0.4149504026237718	0.0
44-45	0.0	0.0	0.0	0.5218141793055853	0.0
46-47	0.0	0.0	0.0	0.6489387423829138	0.0
48-49	0.0	0.0	0.0	0.7822562997393403	0.0
50-51	0.0	0.0	0.0	0.9134963350532578	0.0
52-53	0.0	0.0	0.0	1.038563162012292	0.0
54-55	0.0	0.0	0.0	1.1754026138788762	0.0
56-57	0.0	0.0	0.0	1.3363611073627777	0.0
58-59	0.0	0.0	0.0	1.5090922257542294	0.0
60-61	0.0	0.0	0.0	1.6884120569090668	0.0
62-63	0.0	0.0	0.0	1.8655158645518943	0.0
64-65	0.0	0.0	0.0	2.0460624230080224	0.0
66-67	0.0	0.0	0.0	2.2487890025084596	0.0
68-69	0.0	0.0	0.0	2.4683929753637543	0.0
70-71	0.0	0.0	0.0	2.70022464938353	0.0
72-73	0.0	0.0	0.0	2.9321948248728065	0.0
74-75	0.0	0.0	0.0	3.168458545916601	0.0
76-77	0.0	0.0	0.0	3.429039167819856	0.0
78-79	0.0	0.0	0.0	3.712136171479065	0.0
80-81	0.0	0.0	0.0	4.014643166792571	0.0
82-83	0.0	0.0	0.0	4.323165083067245	0.0
84-85	0.0	3.95718484287404E-5	0.0	4.627076878999972	0.0
86-87	0.0	3.95718484287404E-5	0.0	4.947509921651697	0.0
88	0.0	3.95718484287404E-5	0.0	5.200097030172348	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3835	0.0	37.765156	1
GTATCAA	6060	0.0	34.141766	1
CAACGCA	6210	0.0	30.800617	5
ATCAACG	6480	0.0	29.51726	3
TATCAAC	7415	0.0	27.443111	2
AACGCAG	7265	0.0	27.166641	6
GTACTAG	1450	0.0	25.619165	1
GTACATG	10175	0.0	25.278938	1
TATTCGC	205	1.0841177E-9	25.21712	9
TACATGG	10035	0.0	24.961258	2
ACGCAGA	7570	0.0	24.953222	7
CGCAGAG	7615	0.0	24.68577	8
CGTTAAC	440	0.0	24.56585	9
ACGTTAA	445	0.0	24.289827	8
TCAACGC	8050	0.0	23.935616	4
ACATGGG	10225	0.0	23.808008	3
CATGGGG	7955	0.0	22.685463	4
GCTATTG	1830	0.0	22.085335	8
AGGGCTA	1975	0.0	21.891592	5
GCATCGT	1605	0.0	21.374918	8
>>END_MODULE